ACS Omega
·
2023
·
IF 4.1
Yue Zhao,Chaoyuan Tang,Jingyi Huang,Hongyan Zhang,Jingbin Shi,Shujun Xu,Lisha Ma,Chun Peng,Qi Liu,Yang Xiong
In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefly luciferase and also a substrate for ATP-binding cassette transporters (ABC transporters), was used as the probe to detect its efflux kinetics caused by ABC transporters. First, we established a stable doxorubicin (DOX)-resistant cell line (MCF-7/DOXFluc) that overexpressed luciferase. Then, some kinds of TCMs were chosen for the MDR reversal agents to measure its effect on inhibiting the D-luciferin outflow from MCF-7/DOXFluc, and the ideal reversal agent with the least D-luciferin efflux from MCF-7/DOXFluc was selected to further investigate its effect combined with DOX on MCF-7/DOXFluc tumor-bearing mice. The results indicated that quercetin (Qu) could remarkably increase the retention of D-luciferin in MCF-7/DOXFlucin vitro and in vivo. Also, the combination of Qu and DOX could exceedingly inhibit the tumor growth, which proved the feasibility of this in vitro screening method. The study proposed a feasible method for mass screening of MDR agents from TCMs in vitro.
研究领域:
药理学
·
肿瘤学
·
传统医学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2016
·
IF 8.387
Xiaofeng Shen,Yanxia Zhang,Yong Gu,Yun Xu,Yong Liu,Bin Li,Liang Chen
In this study, a cell-free bone tissue engineering system based on a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was developed, in which two bioactive molecules , stromal cell derived factor-1 (SDF-1) and bone morphogenetic protein-2 (BMP-2), were embedded and released in a sequential and controlled manner to facilitate cell recruitment and bone formation, respectively. BMP-2 was initially loaded into SF microspheres , and these BMP-2 containing microspheres were subsequently encapsulated into the SF/nHAp scaffolds, which were successively functionalized with SDF-1 via physical adsorption. The results indicated rapid initial release of SDF-1 during the first few days, followed by slow and sustained release of BMP-2 for as long as three weeks. The composite scaffold significantly promoted the recruitment of bone marrow mesenchymal stem cells (BMSCs) and osteogenic differentiation of them in vitro . Further, the in vivo studies using D-Luciferin-labeled BMSCs indicated that implantation of this composite scaffold markedly promoted the recruitment of BMSCs to the implanted sites. Enhanced bone regeneration was identified at 12 weeks' post-implantation. Taken together, our findings suggested that the sequential and sustained release of SDF-1 and BMP-2 from the SF/nHAp scaffolds resulted in a synergistic effect on bone regeneration. Such a composite system , therefore, shows promising potential for cell-free bone tissue engineering applications .
研究领域:
生物医学工程
·
组织工程
·
再生医学
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DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xianghua Zhong,Li Luo,Jiyuan Wu,Weirun Li,Xinyang Liu,Tenghui Ye,Zhenhua Li,Peng Shi
Mesenchymal stromal cell (MSC) therapy holds great promise for treating myocardial infarction (MI). However, the inflammatory and reactive oxygen species (ROS)-rich environment in infarcted myocardium challenges MSC survival, limiting its therapeutic impact. In this study, we demonstrate that chemical modification of MSCs with anti-VCAM1 and polydopamine (PD) significantly enhances MSC survival and promotes cardiac repair. Anti-VCAM1 modification facilitates MSC adhesion to inflamed tissue, ensuring MSC retention in the injured myocardium, while PD scavenges ROS surrounding MSCs, creating a conducive environment for cell transplantation. Our data indicate that chemically engineered MSCs effectively disrupt the inflammation-ROS cycle and modulate inflammation-related immune responses, thus improving MI microenvironments. Single-cell RNA sequencing of rat hearts reveals that treatment with engineered MSCs inhibits cardiac fibrosis by suppressing HB-EGF–EGFR signaling between anti-inflammatory macrophages and activated fibrillates. Ultimately, engineered MSCs demonstrate superior therapeutic efficacy in a rat model of MI. This study presents a straightforward, safe, and efficient chemical method for enhancing MSC therapy.
研究领域:
再生医学
·
心血管病学
·
细胞治疗
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产品关联
DOI
PubMed
高影响因子
Matter
·
2024
·
IF 17.3
Pingping Fan,Kui Li,Tian Li,Panke Zhang,Shuo Huang
Alcoholic beverages, such as wine, beer, and distilled spirits, are widely produced and consumed in different nations. Different types of alcoholic beverages contain different combinations of flavor compounds. However, rapid and simultaneous analysis of a large variety of compounds in alcoholic beverages by a miniatured and portable device remains a challenge. In this paper, a Mycobacterium smegmatis porin A (MspA) nanopore modified with a phenylboronic acid (PBA) adapter is applied for rapid analysis of a variety of alcoholic beverages. By utilizing custom machine learning algorithms, various cis -diols are identified simultaneously in both distilled and fermented alcoholic beverages, generating unique barcodes for each sample type. Nanopore analysis of alcoholic beverages has also never been carried out previously. Rapid grading of wine sweetness and detection of additives, including sucrose and D-tartaric acid, are also demonstrated, showcasing the significance of this technique in the administration of wine production.
研究领域:
生物技术
·
食品科学
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2026
·
IF 8.8
Zhixin Su,Miaojia Pan,Wenyong Jiang,Hongyuan Zhang,Minhua Liu,Yuhan Zhao,Zhaoxu Wang,Saisai Lin,Hu Zhou,Tiefan Huang
MXene displays exceptional promise for water purification, owing to its versatile surface chemistry and favorable physicochemical properties. However, its separation performance is substantially hindered by intercalation-induced swelling. Here, inspired by the “brick-and-mortar” structure of nacre, nacre-like nanocomposite membranes are developed through the co-assembly of MXene nanosheets (bricks) and polyetherimide (mortar). Leveraging MXene's unique properties, polyetherimide serves three pivotal functions: it acts as "spacer", precisely defines the interlayer spacing at ∼13.84 Å; it functions as "regulator", abundant hydrophobic groups regulate the microenvironment within the nanochannels; and it serves as "stabilizer", establishes robust non-covalent interactions with the MXene basal planes, thereby reinforcing channel integrity. Consequently, the resulting membranes exhibit outstanding stability in aqueous media, achieving a swelling ratio as low as 1.56%. Remarkably, after 1080 h of static water immersion, the membrane retained a relatively stable interlayer structure and separation performance, demonstrating prolonged aqueous stability. This bioinspired strategy provides critical insights for the rational design of structurally stable two-dimensional membranes.
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Xiaofeng Lu,Dong Zhong,Qi An,Liting Kang,Na Fan,Junjie Cao,Renjie Li,Qianqian Cao,Yudong Zhou,Xiaoyu Du,Shuanghong Yan,Juan Li,Xiaolei Qu,Yuqin Wang
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) make up a large class of emerging chemical pollutants that have caused extensive contamination of global water sources. As the toxicity of PFAS becomes increasingly recognized, there is a growing demand for low-cost and rapid sensors capable of screening water samples for multiple PFAS species. However, most electrochemical and optical sensors can detect only one or two PFASs, despite the high structural diversity of these compounds in aquatic environments. Here, we report a single-molecule nanopore sensor that enables simultaneous detection of nine PFASs in a single measurement. By incorporating a β-cyclodextrin (β-CD) adapter into a mutant α-hemolysin (α-HL) nanopore, translocating PFAS molecules produce distinct current blockades that allow clear discrimination based on carbon chain lengths, hydrogen substitutions, and terminal functional groups. With assistance from a machine learning classifier, an overall identification accuracy of 95.83% is achieved. This strategy allows direct, label-free, and rapid discrimination of multiple PFASs in surface water samples at environmentally relevant concentrations as low as the microgram per liter level, without chemical labeling, separation, or enrichment. The successful demonstration of nanopore sensing in complex real-world matrices highlights its strong potential for practical, field-deployable environmental analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tingting Liu,Yao Gu,AL-Ansi Waleed,Mingcong Fan,Li Wang,Yan Li,Haifeng Qian
Natural α-amylase inhibitors (α-AIs) serve as food processing additives, capable of mitigating postprandial blood glucose levels, but heat resistance limits their application in high-temperature processing. This study delved into the correlation between protein structural characteristics and heat-resistance of colored highland barley (CHB) α-AIs and evaluated the inhibitory activity during chemical modification and in vitro digestion. Results demonstrated that CHB α-AIs were glycoproteins, the inhibitory activity retention rate of black highland barley α-AI salted-out with 0–60 % (NH 4 ) 2 SO 4 (BK1 α-AI) was 56.23 % ± 0.64 %. The protein structure analysis revealed that the preservation of three-dimensional structure was attributed to hydrogen bonds and hydrophobic interactions, and disulfide bonds played a crucial role in maintaining protein folding and activity. Succinylation increased the content of disulfide bonds after heating, and the inhibitory activity retention rate of α-AI noodles increased from 37.72 % ± 2.49 % to 42.79 % ± 0.39 %. These findings provide a theoretical foundation for the application of α-AI in thermally processed foods.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2026
·Vol. 12
·期 1
·
IF 12.5
Yunqi Xiao,Shanyu Zhang,Xinmeng Gao,Tian Li,Hanhan Zhang,Panke Zhang,Shuo Huang
Oligosaccharides, polymers of covalently linked monosaccharides, are essential biomolecules mediating diverse biological and physiological processes. Despite their critical roles, the structural complexity of many oligosaccharides poses a notable challenge for comprehensive characterization, limiting our understanding of their biological functions. Nanopores as emerging single-molecule sensors offer promise for oligosaccharide structural analysis. Here, we demonstrate direct discrimination of diverse mono- and oligosaccharides using a hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore integrated with a single phenylboronic acid (PBA) adapter (MspA-90PBA). The high-resolution event signatures generated by MspA-90PBA enable structural analysis of complex oligosaccharides via hydrolysis into smaller fragments, which can be analyzed for either structure profiling or determination. Using a minimized reference database, we achieve structural determination of raffinose, stachyose, and verbascose through nanopore characterization of its hydrolyzed fragments followed by fragment assembly. This work establishes a framework for nanopore-based oligosaccharide analysis, combining single-molecule sensing with a fragment assembly strategy to address the long-standing challenge of glycan structural elucidation.
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Zhixin Su,Na Tao,Siren Li,Wenyong Jiang,Miaojia Pan,Hongyuan Zhang,Zhaoxu Wang,Hu Zhou,Tiefan Huang
MXene nanomaterials have emerged as promising materials for membrane design due to their distinctive chemical functionalities and tunable lamellar architecture. However, weak interlayer interactions between adjacent nanosheets often lead to membrane swelling and even redispersion in solvents, thereby compromising their performance in liquid separation. Although conventional crosslinking methods can alleviate these issues, they often cause an irreversible alteration of the interlayer spacing, which hinders precise molecular sieving. To address these challenges, we developed an in-situ disulfide crosslinking strategy for fabricating MXene membranes, in which chemical modification and crosslinking occur after membrane formation. As a result, the interlayer spacing of the crosslinked membrane remains virtually identical to that of the pristine membrane. Thanks to the crosslinked structure, the membranes maintain their structural integrity even under sonication. This well-defined microstructure enables size-selective molecular sieving capability. Moreover, leveraging the reversibility of disulfide bonds, the membrane exhibits excellent recyclability, achieving a nanosheet recovery rate of 88.4 %. This in-situ crosslinking strategy not only provides a sustainable pathway for advancing MXene-based membranes in water purification but also shows great potential for application with other two-dimensional materials to prepare high-performance membranes.
研究领域:
材料科学
·
纳米科技
·
膜分离技术
·
水处理
·
高分子化学
·
界面化学
·
环境工程
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jiulong Yin,Lei Qi,Na Li,Meng Li,Chao Jiang,Yujian Yao,Xuan Zhang
Tight ultrafiltration (TUF) membranes with rational structures exhibited considerable promise in the selective dye/salt fractionation during textile wastewater desalination. However, conventional polymeric ultrafiltration membranes suffer from severe fouling and limited dye/salt selectivity. Herein, we employed a controllable interfacial polymerization (IP) strategy using a brominated monomer, 2,6-dibromo-3,5-dihydroxybenzoic acid (DBHBA), as an aqueous monomer to react with trimesoyl chloride (TMC) for the fabrication of a series of polyester-based TUF membranes. The incorporation of bromine substituents into DBHBA induced electron-withdrawing and steric effects, which reduced its reactivity with TMC, thereby enabling it to construct a loose and hydrophilic polyester active layer with precisely tunable pore structures. The optimized DBHBA TUF membrane exhibited an exceptional water permeance of 92.9 L m −2 h −1 bar −1 , a high dye rejection (>98 %), and a low Na 2 SO 4 rejection (<3 %), achieving a separation factor of 32.8 for selective dye/salt fractionation. More importantly, the smooth and hydrophilic membrane surface endowed superior anti-fouling capability with a flux recovery ratio of >94.4 % in cyclic fouling–cleaning tests. These results demonstrate that regulating the IP process using functionalized monomers is an effective strategy for designing advanced TUF membranes featuring excellent dye/salt selectivity and robust fouling resistance, offering a promising route for sustainable textile wastewater desalination.
研究领域:
膜科学
·
环境工程
·
分离技术
·
高分子化学
·
水处理
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Mao Mu,Nigel Graham,Wenzheng Yu,Kening Sun,Xiyan Xu,Ting Liu
The unique layered structure and abundant 2D nanochannels of MXene membranes have demonstrated outstanding permeability, indicating their great potential in the field of water treatment . However, their unstable interlayer spacing and irregular layer structure have limited their ability to separate small molecular pollutants effectively. In this study, we successfully prepared composite MXene membranes with high rejection and low adsorption properties by exploiting the differences in the surface functional groups and chain lengths of dopamine (DA) and polyethylene glycol (PEG) through an optimal cross-linking approach. These composite membranes exhibited excellent stability in terms of interlayer spacing and mitigated the adverse effects of stacking defects. Compared to MXene membranes, the composite membranes achieved a 1.76-fold increase in dye rejection efficiency, reaching 98.7% at most, with an adsorption ratio of only 1.8%. Moreover, outstanding performances were also exhibited in pressure and concentration load tests. Furthermore, in the context of forward osmosis applications, the composite membranes demonstrated excellent Na + rejection of 99.9%. More importantly, these membranes showed no performance deterioration throughout 50 filtration cycles, with only a slight increase in permeance of 19.3% over a 38-day testing period. Thus, this work presents a novel solution for fabricating MXene composite membranes with low swelling, minimal defects, and high stability, enhancing its potential in the field of water and wastewater treatment .
研究领域:
材料科学
·
水处理工程
·
环境科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 7
·903-909
·
IF 2.896
Yiming Liu,Yanan Sun,Minli Yang
A new kind of convenient, low-cost double-potential ratiometric ECL sensing platform for the quantification of Cu2+ was developed with carbon nitride nanosheets (g-C3N4 NSs) and graphene quantum dots (GQDs) as ECL luminophores. g-C3N4 NSs mixed with multi-walled carbon nanotubes (MWCNTs) was immobilized on a glass carbon electrode (GCE) and produced strong cathodic ECL at a potential of −1.2 V (vs. Ag/AgCl), while GQDs in the solution gave anodic ECL at +2.5 V. MWCNTs were used here to amplify the ECL signal of g-C3N4 NSs. The addition of Cu2+ causes the cathodic ECL signal from g-C3N4 to decline, while the anodic ECL signal from GQDs remains unchanged. With the anodic ECL signal as an internal reference, a double-potential ratiometric ECL sensing platform for Cu2+ was established. The ratio of the cathodic signal intensity to anodic signal intensity showed a linear response to the Cu2+ concentration over a range from 5.0 × 10−10 to 1.0 × 10−6 mol L−1 and the detection limit was 0.37 nmol L−1 (3σ/S). Such a construction strategy alleviates the interference from the environment and therefore improves the detection accuracy of Cu2+. Compared with other methods for Cu2+ detection, this method is simpler and more sensitive.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）