JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Yanjing Guo,Weizhe Liu,He Wang,Xiaoxiong Wang,Sheng Qiang,Hazem M. Kalaji,Reto Jörg Strasser,Shiguo Chen
A biocontrol method plays an important role in weed management. In this study, we aimed to clarify the phytotoxicity of the mycotoxin patulin (PAT) and reveal its mode of action as a new natural photosystem II (PSII) inhibitor. Phytotoxicity test showed that PAT has herbicidal activity and causes significant leaf lesions on Ageratina adenophora. Under a half-inhibition concentration I50 (2.24 μM), the observed significant decrease in oxygen evolution rate and the increase in the J-step of the chlorophyll fluorescence rise OJIP curve indicated that PAT strongly reduces photosynthetic efficiency by blocking electron transport from the primary to secondary plastoquinone acceptors (QA to QB) of PSII. Molecular modeling of PAT docking to the A. adenophora D1 protein suggested that PAT bounds to the QB site by forming hydrogen bonds to histidine 252 in the D1 protein. It is proposed that PAT is a new natural PSII inhibitor and has the potential to be developed into a bioherbicide or used as a template scaffold for discovering novel derivatives with more potent herbicidal activity.
研究领域:
植物学
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分子生物学
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农业科学
✓ 已验证
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 80
·期 1
·133-148
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IF 4.1
Qian Yang,Yanjing Guo,He Wang,Zhi Luo,Ying Chen,Mengyun Jiang,Huan Lu,Bernal E. Valverde,Sheng Qiang,Reto Jörg Strasser,Shiguo Chen
BACKGROUND Bioherbicides are becoming more attractive as safe weed control tools towards sustainable agriculture. Natural products constitute an important source chemicals and chemical leads for discovery and development of novel pesticide target sites. Citrinin is a bioactive compound produced by fungi of the genera Penicillium and Aspergillus . However, its physiological-biochemical mechanism as a phytotoxin remains unclear. RESULTS Citrinin causes visible leaf lesions on Ageratina adenophora similar to those produced by the commercial herbicide bromoxynil. Phytotoxicity bioassay tests using 24 plant species confirmed that citrinin has a broad activity spectrum and therefore has potential as a bioherbicide. Based on chlorophyll fluorescence studies, citrinin mainly blocks PSII electron flow beyond plastoquinone Q A at the acceptor side, resulting in the inactivation of PSII reaction centers. Furthermore, molecular modeling of citrinin docking to the A. adenophora D1 protein suggests that it binds to the plastoquinone Q B site by a hydrogen bond between the O1 hydroxy oxygen atom of citrinin and the histidine 215 of the D1 protein, the same way as classical phenolic PSII herbicides do. Finally, 32 new citrinin derivatives were designed and sorted according to free energies on the basis of the molecular model of an interaction between the citrinin molecule and the D1 protein. Five of the modeled compounds had much higher ligand binding affinity within the D1 protein compared with lead compound citrinin. CONCLUSION Citrinin is a novel natural PSII inhibitor that has the potential to be developed into a bioherbicide or utilized as a lead compound for discovery of new derivatives with high herbicidal potency. © 2023 Society of Chemical Industry.
研究领域:
植物学
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农业科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2021
·
IF 7.086
Li Yang,Yu-Xi Feng,Yu-Juan Lin,Xiao-Zhang Yu
Various environmental contaminants can find their way to enter plant cells and disturb and/or damage the essential components of PSII repair cycle in chloroplast, thereby resulting in dysfunction of chloroplast. In the current research, a microcosm hydroponic experiment was set up to evaluate the comparative effects of sodium hydrosulfide (NaHS)- and proline (Pro)-mediated functional repairing of chloroplast in rice plants under SCN − stress. Our results displayed that when exposed to environmental realistic SCN − concentrations (24–300 mg L −1 ), foist significant ( p < 0.05) gene-dose repercussion on the pathways of photosynthetic reactions and energy metabolism in rice shoots, and a downturn in the level of total soluble starch, sugar, and chlorophyll. Sodium hydrosulfide application effectively mitigated ( p < 0.05) the toxic effects of SCN − in rice seedlings by stimulating the processes of phosphorylation, dephosphorylation and new-synthesis of D1 protein in the PSII repair cycle, and increased the turnover of D1 protein to recover CO 2 assimilation. Evidently, Pro treatment mainly enhanced ( p < 0.05) the expression of magnesium chelatase (MgCh) and ribulose-1,5-bisphosphate carboxylase (Rubisco) related genes under simulated SCN − stress, suggesting that the targeted repairing site in chloroplast by Pro was different from NaHS. The outcome of the present research contributes to a better understanding at molecular level for repairing of chloroplast functional disorder by NaHS and Pro at different key nodes under SCN − stress.
研究领域:
植物学
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环境科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
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2022
·Vol. 11
·期 20
·2797
·
IF 4.658
Mengyun Jiang,Qian Yang,He Wang,Zhi Luo,Yanjing Guo,Jiale Shi,Xiaoxiong Wang,Sheng Qiang,Reto Jörg Strasser,Shiguo Chen
Biological herbicides have received much attention due to their abundant resources, low development cost, unique targets and environmental friendliness. This study reveals some interesting effects of mycotoxin cytochalasin A (CA) on photosystem II (PSII). Our results suggested that CA causes leaf lesions onAgeratina adenophoradue to its multiple effects on PSII. At a half-inhibitory concentration of 58.5 μΜ (I50, 58.5 μΜ), the rate of O2evolution of PSII was significantly inhibited by CA. This indicates that CA possesses excellent phytotoxicity and exhibits potential herbicidal activity. Based on the increase in the J-step of the chlorophyll fluorescence rise OJIP curve and the analysis of some JIP-test parameters, similar to the classical herbicide diuron, CA interrupted PSII electron transfer beyond QAat the acceptor side, leading to damage to the PSII antenna structure and inactivation of reaction centers. Molecular docking model of CA and D1 protein ofA. adenophorafurther suggests that CA directly targets the QBsite of D1 protein. The potential hydrogen bonds are formed between CA and residues D1-His215, D1-Ala263 and D1-Ser264, respectively. The binding of CA to residue D1-Ala263 is novel. Thus, CA is a new natural PSII inhibitor. These results clarify the mode of action of CA in photosynthesis, providing valuable information and potential implications for the design of novel bioherbicides.Keywords:natural product;bioherbicide;photosynthetic inhibitor;chlorophyllafluorescence;D1 protein;molecular docking
研究领域:
植物科学
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生物化学
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分子生物学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
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2016
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IF 3.698
Liqiao Jin,Xingkai Che,Zishan Zhang,Yuting Li,Huiyuan Gao,Shijie Zhao
Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) that is widely distributed in the environment and seriously affects the growth and development of plants. To clarify the mechanisms of the direct effects of phenanthrene on the plant photosynthetic apparatus, we measured short-term phenanthrene-treated cucumber leaves. Phenanthrene inhibited Rubisco carboxylation activity, decreasing photosynthesis rates (Pn). And phenanthrene inhibited photosystem II (PSII) activity, thereby blocking photosynthetic electron transport. The inhibition of the light and dark reactions decreased the photosynthetic electron transport rate (ETR) and increased the excitation pressure (1−qP). Under high light, the maximum photochemical efficiency of photosystem II (F v /F m ) in phenanthrene-treated cucumber leaves decreased significantly, but photosystem I (PSI) activity (Δ I/I o ) did not. Phenanthrene also caused a J-point rise in the OJIP curve under high light, which indicated that the acceptor side of PSII Q A to Q B electron transfer was restricted. This was primarily due to the net degradation of D1 protein, which is caused by the accumulation of reactive oxygen species (ROS) in phenanthrene-treated cucumber leaves under high light. This study demonstrated that phenanthrene could directly inhibit photosynthetic electron transport and Rubisco carboxylation activity to decrease net Pn. Under high light, phenanthrene caused the accumulation of ROS, resulting in net increases in D1 protein degradation and consequently causing PSII photoinhibition .
研究领域:
植物生理学
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环境科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）