Frontiers in Environmental Science
·
2023
·
IF 4.6
Zhang Meilin,Li Shaofeng,Sun Jian,Sun Jianping,Wang Lei,Zhao Rui
To reduce the hazard of sulfamethoxazole (SMX) to the water ecology, the removal mechanism of SMX in wastewater was revealed. This study evaluated the PVDF/DA modified membrane in the membrane bioreactor (MBR) toward the removal and degradation of SMX as a recalcitrant micropollutant. A strategy of dopamine for surface modification of polyvinylidene fluoride (PVDF) membrane was proposed, and PVDF/DA membrane was prepared. Subsequently, PVDF/DA was run in the MBR for 60 days, and the reactor could produce good removal ability for SMX after certain acclimation. When the SMX concentration increased from 0.5 to 2 mg/L, liquid chromatography was used for analysis, and the results showed that the average removal rate was above 85%. The removal effect of COD and NH4+-N and the degree of membrane fouling were also evaluated. By measuring the extracellular polymer (EPS) content, the results showed that after 30 days of operation, the protein and polysaccharide contents in the cake layer on the surface of the PVDF membrane were 1.35 times and 1.49 times that of PVDF/DA membrane, respectively, and the PVDF/DA membrane could alleviate membrane fouling, thereby improving the service life of the membrane and reducing its operating cost. Finally, degradation by-products are evaluated to achieve deeper insight into the degradation mechanisms in the MBR process.
研究领域:
环境科学
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污水处理
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材料科学
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DOI
高影响因子
Coatings
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2023
·Vol. 13
·期 11
·1845
·
IF 3.4
Zhimin Cao,Mingyi Zhu,Xuxu Dong,De Liu,Pan Cao
An innovative antifouling composite coating comprising dopamine (DA), polyethyleneimine (PEI), and silica (SiO2) was developed through a straightforward and environmentally friendly approach. Initially, silica nanoparticles comodified with DA and PEI were meticulously deposited onto 304 stainless steel surfaces pretreated with dopamine to achieve a uniformly distributed nanocomposite surface. Comprehensive analytical techniques, including Fourier transform infrared spectrometry (FTIR), X-ray photoelectron spectrometry (XPS), field emission scanning electron microscopy (SEM), contact angle measurement (CA), and 3D optical profilometry, were employed to affirm the successful preparation of the silica nanocomposite coatings and the effective grafting of MAG II. The antibacterial and antibiofilm performance of the DA/PEI/SiO2-modified surface was rigorously assessed usingVibrio natriegens(V. natriegens), yielding compelling results indicating a substantial 51.4% reduction in biofilm formation on the SS-DA/PEI/SiO2sample surfaces, coupled with an impressive 95.2% decrease inV. natriegensadhesion. This pioneering research introduces an innovative strategy for the development of antimicrobial surfaces with promising applications in medical devices, aquaculture, and related fields.Keywords:surface modification;DA/PEI/SiO2nanocoating;antimicrobial performance;stability properties
研究领域:
材料科学
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生物医学工程
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表面化学
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Wenwen Lv,Xinxin Jiao,Zhiwen Zhang,Luocheng Zhang,Jingyi Song,Hua Wu,Junsong Xiao
DHA with varying degrees of oxidation was obtained by the Schaal oven acceleration method. Then, the temporal changes in free radicals, volatile compounds, and carbonyl compounds in oxidized DHA was analyzed using HS-SPME-GC-MS, the content of which were increased with the oxidation time. RAW264.7 macrophages were used to evaluate the effects of DHA oxidation products on the oxidative stress, gene and protein expression of inflammatory factors at different oxidation times. With the increase of oxidation time, oxidized DHA stimulated cells with significantly higher levels of reactive oxygen species (ROS), nitric oxide (NO) and malondialdehyde (MDA), while superoxide dismutase (SOD) activity was significantly inhibited. It also stimulated a significant increase in the expression of tumor necrosis factor-α (TNF-α), interleukin (IL-1β) and inducible nitric oxide synthase (iNOS), which exacerbated the cellular inflammatory response. Data on DHA oxidation products were analyzed using OPLS-DA. The compound (E,E)-2,4-heptadienal was identified as a potential differential metabolite capable of distinguishing among six oxidation time points. Furthermore, the pro-inflammatory effects of oxidized DHA were accurately predicted using partial least squares regression (PLSR) and support vector regression (SVR) models. This study established a correlation between volatile oxidation products and their potential pro-inflammatory effects, which were readily detectable through high-throughput assays. It thereby provided a rapid and reliable method for assessing the predicted bioactivity of DHA at varying levels of oxidation.
研究领域:
营养学
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生物化学
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免疫学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Daliang Yu,Yingying Zha,Zheng Zhong,Yiming Ruan,Ziwei Li,Lili Sun,Sen Hou
Dichlorodihydrofluorescein diacetate (DCFH-DA) has been widely used in detection of reactive oxygen species (ROS). Unfortunately, the detection process is easily affected by light irradiation during measurement, which cannot be overlooked especially for samples with a small amount of ROS. In the study, we revealed several factors that influence the ROS detection process and developed a new ROS detection method by DCFH-DA with higher stability and sensitivity. After light irradiation, the fluorescence signal of DCFH-DA solution increased significantly with pH value higher than 7.4. The increase of fluorescence signal was attributed to a self-oxidization process of DCFH-DA, which transferred dichlorodihydrofluorescein (DCFH) to 2′,7′-dichlorofluorescein (DCF) with the presence of DCF. At the beginning stage of light irradiation, i.e., when the amount of DCF was negligible compared to DCFH (DCFH-DA), the ratio of fluorescence density between samples keeps unchanged after light irradiation. On this basis, we developed a stable and sensitive ROS detection method via light irradiation. The method was successfully applied to detect ROS in HeLa cells which was induced by hydrogen peroxide (H 2 O 2 ) and plastic nanoparticles. A new insight into the proportional increase of fluorescence signal by light irradiation was revealed.
研究领域:
生物化学
·
细胞生物学
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产品关联
DOI
高影响因子
CARBON
·
2020
·
IF 8.821
Meng Zhou,Qiming Yan,Qi Fu,Heqing Fu
The combination of microwave absorption performance and self-healing function is of great practical significance. In this paper, first, ZnO@MWCNTs nanoparticle is prepared by loading Zinc oxide (ZnO) on the surface of the multiwalled carbon nanotubes (MWCNTs) as microwave absorbers. Then, the thermoreversible self-healing DA-PDMS is prepared by using polydimethylsiloxane (PDMS) as raw material based on Diels-Alder (DA) reaction. Finally, a kind of novel multifunctional ZnO@MWCNTs/DA-PDMS nanocomposite with self-healing and microwave absorption properties is synthesized with the addition of ZnO@MWCNTs nanoparticle. When the cracked ZnO@MWCNTs/DA-PDMS nanocomposite is maintained at 120 °C for 10 min, it can recover more than 90% of the original mechanical strength . Even after three healing cycles, the ZnO@MWCNTs/DA-PDMS nanocomposite also displays excellent healing efficiency (about 90%). On the other hand, when the content of ZnO@MWCNTs nanoparticle is 10 wt%, ZnO@MWCNTs/DA-PDMS nanocomposite exhibits an effective absorption bandwidth from 8.2 to 12.4 GHz (full X-band) at a thickness of 3 mm and the minimum reflection loss of −20.1 dB. Even after the 3rd healing cycle, the minimum reflection loss of the healed samples can still maintain −20.0 dB at a thickness of 3 mm. ZnO@MWCNTs/DA-PDMS nanocomposite will have good development prospect in the field of intelligence and microwave absorption materials .
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2024
·
IF 10.8
Jianglin Hu,Jiayin Dai,Nan Sheng
Following its introduction as an alternative to perfluorooctanoic acid, hexafluoropropylene oxide dimer acid (HFPO-DA) has been extensively detected in various environmental matrices. Despite this prevalence, limited information is available regarding its hepatotoxicity biomarkers. In this study, toxicokinetic simulations indicated that under repeated treatment, HFPO-DA in mice serum reached a steady state by the 4th day. To assess its subacute hepatic effects and identify potential biomarkers, mice were administered HFPO-DA orally at doses of 0, 0.1, 0.5, 2.5, 12.5, or 62.5 mg/kg/d for 7 d. Results revealed that the lowest observed adverse effect levels were 0.5 mg/kg/d for hepatomegaly and 2.5 mg/kg/d for hepatic injury. Serum metabolomics analysis identified 34, 58, and 118 differential metabolites in the 0.1, 0.5, and 2.5 mg/kg/d groups, respectively, compared to the control group. Based on weighted gene coexpression network analysis, eight potential hepatotoxicity-related metabolites were identified; among them, kynurenic acid (KA) in mouse serum exhibited the highest correlation with liver injury. Furthermore, liver-targeted metabolomics analysis demonstrated that HFPO-DA exposure induced metabolic migration of the kynurenine pathway from KA to nicotinamide adenine dinucleotide, resulting in the activation of endoplasmic reticulum stress and the nuclear factor kappa-B signaling pathway. Notably, pretreatment with KA significantly attenuated liver injury induced by HFPO-DA exposure in mice, highlighting the pivotal roles of KA in the hepatotoxicity of HFPO-DA.
研究领域:
毒理学
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环境科学
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代谢学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jingxuan Zhao,Hongxu Liu,Yuchao Qi,Ruijia Wang,Ziwei Lv,Yang Yu,Shulin Sun
With the rapid development of industry, the problem of water pollution has become increasingly serious. Though there are many separation materials available to solve this problem, how to separate multiple wastewater at the same time remains a challenge. Hydrogel coating is suitable for use in the field of oil–water separation, and chitosan (CS) is effective in removing anionic dyes. Therefore, the preparation of separation membranes with hydrogel coating containing CS and dopamine (DA) can effectively separate oil–water emulsion and anionic dye wastewater. In this paper, DA@CNTs/CS hydrogel coating was prepared by sequentially coating Cu 2+ , DA@CNTs, Cu 2+ and CS on the surface of polyvinylidene fluoride (PVDF) membrane by deposition of self-assembly, which enhanced the hydrophilicity of the membrane and made it highly underwater oleophobic and antifouling. The alkali treated PVDF membrane contained a large amount of –OH, Cu 2+ , which formed coordination bonds with –OH, CS, and DA and the hydrogel coating could be stably loaded on the membrane. In addition, the introduction of rigid CNTs effectively improved the performance of the modified membrane under extreme conditions such as high pH and high salt concentration. PVDF-DA@CNTs/CS membrane had an ideal pure water flux (PWF) of 1470 (L·m −2 ·h −1 ) and the separation efficiency of 99.7 % for anionic dye and 99.4 % for oil–water emulsion. The contaminant on the membrane surface can be quickly removed by washing with DI water. The overall performance of the membrane is also maintained at a very high level of long-term filtration of wastewater. In summary, this study provides a new approach to complex wastewater treatment technology.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
Journal of Coatings Technology and Research
·
2023
·Vol. 20
·期 3
·979-994
·
IF 2.3
Liu De,Wang Huming,Dong Xuxu,Liu Xiaodan,Dosta Sergi,Zhang Chao,Cao Pan
Marine fouling can cause a series of hazards in the marine industrial field, and the traditional antifouling methods do not fulfill the green antifouling requirement. Herein, a novel DA/PEI/SiO 2 /antibacterial peptide antifouling coating was prepared by a multi-modification approach. Initially, the nanocoatings were prepared by depositing DA, PEI, and SiO 2 on the dopamine (DA)-modified 304 stainless steel (SS) surface, and finally, the DA/PEI/SiO 2 /AMPs composite coatings were prepared by grafting antimicrobial peptides (AMPs). The surfaces of SS before and after modification were characterized by Fourier transform infrared spectrometer (FTIR), X-ray photoelectron spectrometer (XPS), field emission scanning electron microscopy (SEM), contact angle measurement, and 3D optical profilometer. SSN-1 cells were used to evaluate the cytocompatibility of the modified surface. The results revealed that the cells cultured on the modified surface still maintained a good adhesion morphology, demonstrating the superior cytocompatibility of the composite coating. The anti-biofilm and antimicrobial properties of the modified samples were evaluated using Vibrio natriegens . The antibacterial efficiency of SS-DA/PEI/SiO 2 surfaces before and after AMPs modification reached 78.39 and 95.90%, and anti-biofilm efficiency of AMPs modified surface achieved 72.87% corresponding to 44.19% of SS-DA/PEI/SiO 2 . The successful grafting of AMPs improved the antibacterial and anti-biofilm properties of the modified sample surfaces. Electrochemical and stability tests indicated that the modified sample surfaces exhibited excellent corrosion resistance and antifouling stability properties. This research could provide a novel green anti-fouling and anti-corrosion strategy for the marine industry and other related fields.
研究领域:
材料科学
·
表面工程
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生物医学工程
·
海洋技术
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Zhiwen Tang,Timothy M. Vogel,Qing Wang,Changlong Wei,Mukhtiar Ali,Xin Song
In this study, the biodegradability of trifluoroacetate (TFA), perfluorooctanoic acid (PFOA), and perfluoro-2-methyl-3-oxahexanoic acid (HFPO-DA) by a native microbial community was evaluated over a 10-month incubation period. The observed microbial defluorination ratios and removal efficiency were 3.46 ( ± 2.73) % and 8.03 ( ± 3.03) %, 8.44 ( ± 1.88) % and 13.52 ( ± 4.96) %, 3.02 ( ± 0.62) % and 5.45 ( ± 2.99) % for TFA, PFOA and HFPO-DA, respectively. The biodegradation intermediate products, TFA and pentafluoropropionic acid (PFA), of PFOA and HFPO-DA were detected in their biodegradation treatment groups. Furthermore, the concentrations of the PFOA metabolites, perfluorohexanoic acid (PFHxA) and perfluoroheptanoic acid (PFHpA), in the aqueous solutions after incubation were quantified to be 0.21 and 4.14 µg/L. TFA, PFOA and HFPO-DA significantly reduced the microbial diversity and changed the structure of the community. The co-occurrence network analysis showed that low abundance species, such as Flexilinea flocculi , Bacteriovorax stolpii , and g_Sphingomonas , are positively correlated with the generation of fluoride ion, implying their potential collaborative functions contributing to the observed biodefluorination. The findings in this study can provide insights for the biodegradation of perfluoroalkyl carboxylic acids and their emerging alternatives by indigenous microorganisms in the environment.
研究领域:
环境微生物学
·
生物修复
·
化学生态学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ligang Yu,Ying Pang,Guang Shen,Baoqing Bai,Yukun Yang,Maomao Zeng
This work aimed to investigate the effects of frozen storage on volatile compounds of white meats (chicken and duck) and red meats (pork, beef, and mutton). The samples were stored at −18 °C for 0, 2, 4, 10, 18 weeks, and volatile compounds were analyzed by headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry. Results indicated that the total amounts of volatile compounds increased with frozen storage duration of meats. The correlations were observed between frozen storage duration and levels of 2-ethyl-1-hexanol, tetradecane, nonanal, decanal, octanal, tridecanal, benzaldehyde, pentadecane, propanoic acid,2-methyl-,3-hydroxy-2,2,4-trimethylpentyl ester, heptadecane, and hexanal ( r = 0.7456–0.9873). Levels of octanal and propanoic acid,2-methyl-,3-hydroxy-2,2,4-trimethylpentyl ester in white meat and benzaldehyde in red meat versus frozen storage duration fitted very well with zero-order reactions. Therefore, it was concluded that changes in volatile compounds derived from lipid oxidation may be used as indicators of quality deterioration during frozen storage of meat.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yuying Xu,Minting Liang,Jintao Huang,Yapei Fan,Haiyue Long,Qunjie Chen,Zhe Ren,Chaoxi Wu,Yifei Wang
Single helical β -glucan is a one-dimensional host that can form a hybrid helix with DNAs/RNAs as delivery systems. However, unmodified β -glucan has a gelling tendency and a single helical conformation is challenging to obtain. Therefore, in this study, we developed a β -glucan formyl derivative with stable single helical conformation and no gelling tendency. Circular dichroism studies found that the formyl- β -glucan could form a hybrid helix with DNA CpG-poly(dA). The hybrid helix delivery system showed improved activation on antigen-presenting cells, thereby upregulating the mRNA and protein levels of inflammatory factors, and had an immune-enhancing effect on ovalbumin (OVA) immunized mice. These results indicate that formyl- β -glucan can be developed as a non-cationic supramolecular DNA delivery platform with low toxicity and high efficiency.
研究领域:
生物技术
·
免疫学
·
药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Neural Regeneration Research
·
2021
·
IF 5.135
Ling-Yu Zhang,Qian-Qian Jin,Christian Hölscher,Lin Li
Patients with Parkinson’s disease (PD) have impaired insulin signaling in the brain. Incretin hormones, including glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), can re-sensitize insulin signaling. In a recent phase II clinical trial, the first GLP-1 mimic, exendin-4, has shown reliable curative effect in patients with PD. DA-CH5 is a novel GLP-1/GIP receptor unimolecular co-agonist with a novel peptide sequence added to cross the blood-brain barrier. Here we showed that both exendin-4 and DA-CH5 protected against 6-hydroxydopamine (6-OHDA) cytotoxicity, inhibited apoptosis, improved mitogenesis and induced autophagy flux in SH-SY5Y cells via activation of the insulin receptor substrate-1 (IRS-1)/alpha serine/threonine-protein kinase (Akt)/cAMP response element-binding protein (CREB) pathway. We also found that DA-CH5 (10 nmol/kg) daily intraperitoneal administration for 30 days post-lesion alleviated motor dysfunction in rats and prevented stereotactic unilateral administration of 6-OHDA induced dopaminergic neurons loss in the substantia nigra pars compacta. However, DA-CH5 showed curative effects in reducing the levels of α-synuclein and the levels of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β). It was also more effective than exendin-4 in inhibiting apoptotic process and protecting mitochondrial functions. In addition, insulin resistance was largely alleviated and the expression of autophagy-related proteins was up-regulated in PD model rats after DA-CH5 treatment. These results in this study indicate DA-CH5 plays a therapeutic role in the 6-OHDA-unilaterally lesioned PD rat model and is superior to GLP-1 analogue exendin-4. The study was approved by the Animal Ethics Committee of Shanxi Medical University of China.
研究领域:
神经科学
·
药理学
·
分子生物学
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）