JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2023
·Vol. 170
·期 8
·084510
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IF 3.9
Jinyi He,Tianhui Li,Yingying Liu,Ao Wang,Wenxing Jiang,Chengwei Deng,Junbo Hou,Xiaodong Zhuang,Junliang Zhang,Changchun Ke
Poly[2,2-(m-phenylene)−5,5-bibenzimidazole] (m-PBI), the most studied PBI, is close to practical application, however, the high cost of m-PBI restricts its application for HT-PEMFCs. The cost can be reduced by 85% if the low cost & high impurity industrial DAB can be used to synthesize m-PBI after pre-treatment. Herein, we study the influence of impurities in industrial 3,3'-diaminobenzidine (DAB) on the synthesis of polybenzimidazole (PBI), and the possibility of the synthesis of m-PBI based on purified low cost & high impurity industrial DAB. It is shown that impurities would reduce the molecular weight and thermal stability of PBI, and mechanical strength and oxidation stability of the according PBI and H3PO4/PBI membranes. Using industrial DAB (94.40%) without any treatment as a reactant, the molecular weight of the obtained PBI is too low to prepare a membrane. A simple method of purifying low cost & high impurity industrial DAB is proposed, by dissolution and recrystallization using the absorption effect of carbon powder. It is proven that the performances of PBI synthesized using purified DAB are near to the PBI synthesized using high-purity DAB. The peak power density of HT-PEMFC based on PBI from purified industrial DAB reached 426 mW cm−2 at 180 °C.
研究领域:
材料科学
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化学工程
·
能源技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Guang-Li Wang,Xue-Lian Hu,Xiu-Ming Wu,Zai-Jun Li
In this paper, a novel and sensitive assembled quantum dots (QDs)–bienzyme (glucose oxidase (GOD) and horseradish peroxidise (HRP)) hybrid system was designed for the direct determination of glucose. Using o-phenylenediamine (OPD) or 3,3′-diaminobenzidine (DAB) as substrate of HRP, whose corresponding oxidation product (oxOPD or oxDAB) effectively quenched the fluorescence of thioglycollic acid (TGA)-capped CdS QDs. The fluorescence quenching of the CdS QDs was proportional to the concentration of glucose and a detection limit of 0.1 μmol/L (or 20 nmol/L) was achieved under the optimum conditions when using OPD (or DAB) as the substrate of HRP. The sensing system was also applied to the determination of glucose in human serum with satisfactory results without any need for complicated sample pretreatments. Other sugars such as fructose, sucrose and lactose hardly interfered on the detection of glucose. The developed method manifested several advantages of high sensitivity, short analysis time, low cost and easy of operation. Moreover, it had great potential in the direct detection of real samples in clinical diagnostics of various diseases.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Yanrong Wang,Shigui Qiu,Shengwen Tan,Huaizhu Wang,Xuemei Wang,Chenxi Zheng,Lubin Ni,Guowang Diao,Caixing Wang,Zhong Jin
To synergistically combine the features of high specific capacity of n-type organic materials with the high voltage of p-type organic materials, bipolar phenolthiazine-based cathodes with p-type/n-type structures are gaining increased attention for aqueous zinc-ion batteries (AZIBs). Nevertheless, their small molecular structure often leads to dissolution during cycling. To address the challenge, a bipolar poly(phenothiazine iodide-3,3′-diaminobenzidine), abbreviated as P(PTD-DAB), was synthesized as a cathode material. Through comprehensive spectroscopic analyses, we elucidated a cooperative p-type/n-type dual-ion storage mechanism. Specifically, the N atom in the phenothiazine core stores Zn2+ or H+ cations via n-type reactions, while the N atoms connecting phenothiazinium tetraiodide and 3,3′-diaminobenzidine monomers, along with S atoms, act as p-type active sites for storing OTF– anions. The material delivers an average operating voltage of 1 V and achieves a high specific capacity of 211.8 mAh g–1 at a low current density of 0.1 A g–1. Additionally, it shows 90% capacity over 3600 cycles at a high current density of 3 A g–1 while sustaining robust stability at a high mass loading of active cathode (10 mg cm–2). Furthermore, flexible belt-shaped cells maintain 88% capacity retention over 550 cycles at 3 A g–1 even when subjected to repeated bending conditions. This work represents a great advancement in developing bipolar polymers for high-performance AZIBs and offers valuable insights into their design principle and functionality.
研究领域:
材料科学
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电化学
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有机化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 11
·1516-1521
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IF 2.378
Fujuan Luan,Zuhong Yu,Ling Yin,Xia Leng,Yuxue Shi,Jie Wang,Haibin Shi,Weichang Chen
Matrix metalloproteinase-2 (MMP-2) plays an important role in tumorigenesis and metastasis of gastric cancer. Therefore, specific and sensitive detection of MMP-2 is of great significance for clinical diagnosis and therapeutic evaluation of gastric cancer. Herein, we developed a new approach for accurate detection of MMP-2 activity both in gastric cancer cells and clinical tissues using a fluorescent probe Dab-PLGVRGY-FITC. The capability of the probe for detecting MMP-2 was systematically evaluated in gastric cancer MGC-803 cells and clinical gastric cancer tissues. Around 40-fold fluorescence enhancement at a wavelength of 520 nm was determined for the assays of the probe with recombinant MMP-2. The detection limit of MMP-2 was as low as 42 ng mL−1. Strong fluorescence signals were detected in MGC-803 cells and mouse gastric cancer tissues. Fluorescence signal enhancements of 11 clinical gastric cancer tissue lysates were 5.8 ∼ 9 times (7.53 ± 0.77 on average) higher than those from gastritis patients. This probe could be potentially used for early diagnosis of gastric cancer in clinics.
研究领域:
肿瘤学
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生物医学工程
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临床诊断
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Lin Fan,Ting Huang,Doudou Lou,Zengzhou Peng,Yongqi He,Xinyu Zhang,Ning Gu,Yu Zhang
The immunohistochemical method serves as one of the most practical tools in clinical cancer detection and thus has great application value to overcome the existing limits of the conventional method and further improve the detecting efficiency and sensitivity. This study employed 3,3′-diaminobenzidine (DAB), a conventional color indicator for immunohistochemistry, as a novel high-sensitive scattering reagent to provide a multidimensional image signal varying with the overexpression rate of tumor markers. Based on the scattering properties of DAB aggregates, an efficient and robust artificial intelligence-aided immunohistochemical method based on dark-field imaging has been established, with improvement in both the imaging quality and interpretation efficiency in comparison with the conventional manual-operated immunohistochemical method. Referencing the diagnosis from three independent pathologists, this method succeeded in detecting HER2 overexpressed breast tumors with a sensitivity of 95.2% and a specificity of 100.0%; meanwhile, it was found to be applicable for non-small-cell lung tumors and malignant lymphoma as well. As demonstrated, this study provided an effective and reliable means for making diagnostic suggestions, which exhibited great potential in multiple tumor pathological detection at low cost.
研究领域:
病理学
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医学影像学
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生物医学工程
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2015
·Vol. 8
·期 16
·8553-8558
·
IF 7.394
Lin Fan,Yanyan Tian,Rong Yin,Doudou Lou,Xizhi Zhang,Meng Wang,Ming Ma,Shouhua Luo,Suyi Li,Ning Gu,Yu Zhang
Conventional immunohistochemistry is limited to subjective judgment based on human experience and thus it is clinically required to develop a quantitative immunohistochemical detection. 3,3′-Diaminobenzidin (DAB) aggregates, a type of staining product formed by conventional immunohistochemistry, were found to have a special optical property of dark-field imaging for the first time, and the mechanism was explored. On this basis, a novel immunohistochemical method based on dark-field imaging for detecting HER2 overexpressed in breast cancer was established, and the quantitative analysis standard and relevant software for measuring the scattering intensity was developed. In order to achieve a more sensitive detection, the HRP (horseradish peroxidase)-labeled secondary antibodies conjugated gold nanoparticles were constructed as nanoprobes to load more HRP enzymes, resulting in an enhanced DAB deposition as a dark-field label. Simultaneously, gold nanoparticles also act as a synergistically enhanced agent due to their mimicry of enzyme catalysis and dark-field scattering properties.
研究领域:
医学影像
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癌症研究
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）