DYES AND PIGMENTS
·
2019
·
IF 4.018
Muhammad Sohail Haroone,Yuehua Hu,Ping Zhang,Ruili Ma,Xiaorang Zhang,Qari Muhammad Kaleem,Muhammad Bashir Khan,Jun Lu
Nucleic acids , the hereditary material of living beings, perform a vital role in major life activities including growth, heredity, and variation. The genetic information is coded in specific nitrogenous bases , which are the key players in genetic transformation and identification of hereditary diseases. Telomeres are specialized DNA structures present at the end of eukaryotic chromosomes, consisting of repetitive non-transcribed sequences (TTAGGG) and some binding proteins . They protect the chromosomal ends from sticking to each other, and play a key role in the maintenance of genomic integrity. Currently, a number of methods are reported for nucleic acids and telomeres detection but they are not easy going. Therefore, development of sensitive and fast DNA-biosensors to measure the nucleic acids and telomeres sequences is of great importance. This paper describes a systematic study on the preparation of simple ultrathin films (UTFs) composite of fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI), having binding properties in the minor groove of AT-rich DNA segments, blending with single-stranded DNA (ssDNA) and incorporation into layered double hydroxides (LDH) nanosheets using layer-by-layer self-assembly method. The resulting UTFs exhibits excellent sensitivity, selectivity and reversibility for long complementary ssDNA sequence on the basis of DNA hybridization and fluorescence enhancement strategy. The dynamic range was 3–20 μg/mL with a detection limit of 20 μg/mL. This new (ssDNA @DAPI/LDHs) n UTFs, could be an efficient hybrid composite with potential applications in the field of bioluminescent sensoring materials.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yu Ling,Jiao Zhou,Xiao Fang Zhang,Xiao Hu Wang,Nian Bing Li,Hong Qun Luo
As one of critical base excision repair enzymes, uracil-DNA glycosylase (UDG) can specifically repair uracil-induced DNA damage to maintain the genome integrity of organisms. Moreover, abnormal expression of UDG is related to various cancers and other serious diseases. Therefore, it is essential to accurately and sensitively monitor UDG activity. In this work, a ratiometric fluorescence method using poly-(thymine) DNA template copper nanoclusters (Cu NCs) and 4′,6-diamidino-2-phenylindole (DAPI) as the output signals is developed for simple, selective, and sensitive detection of UDG. A double-stranded DNA (dsDNA) consisting of a uracil-containing single chain and a trigger sequence is designed as a substrate dsDNA. Meanwhile, another dsDNA comprising 30 pairs of A-T bases ((AT) 30 dsDNA) is designed to be partially complementary to the trigger chain. The (AT) 30 dsDNA itself has no blunt or recessed 3′ end and cannot be cut by exonuclease III (Exo III). With the Exo III-assisted amplification, adding UDG results in the success synthesis of red-emissive Cu NCs, while the dsDNAs in the system are hydrolyzed and the fluorescence signal of DAPI is weak. In contrast, without addition of UDG, the fluorescence intensity of Cu NCs is low, while the DAPI emits significantly enhanced fluorescence signal. The proposed method presents a detection limit of 5.0 × 10 −5 U mL −1 and can be applied to the HeLa cell lysate sample with satisfactory results. Additionally, the kinetic parameter is studied and the UDG inhibition effect is evaluated. In summary, this work provides a potential platform for DNA repair enzyme-related analysis and clinical diagnosis.
研究领域:
生物化学
·
分子生物学
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Micromachines
·
2017
·Vol. 8
·期 2
·36
·
IF 1.833
Jing Sun,Jiayu Zhang,Haibo Yang,Gongzhuo Wang,Yanzhao Li,Xuxin Zhang,Qidan Chen,Ming-Fei Lang
Single-cell cell cycle analysis is an emerging technique that requires detailed exploration of the image analysis process. In this study, we established a microfluidic single-cell cell cycle analysis method that can analyze cells in small numbers and in situ on a microfluidic chip. In addition, factors that influenced the analysis were carefully investigated. U87 or HeLa cells were seeded and attached to microfluidic channels before measurement. Cell nucleic DNA was imaged by 4′-6-diamidino-2-phenylindole (DAPI) staining under a fluorescent microscope and subsequently fluorescent intensities of the cell nuclei DNA were converted to depict histograms for cell cycle phases. DAPI concentration, microscopic magnification, exposure time and cell number were examined for optimal cell cycle analysis conditions. The results showed that as few as a few hundred cells could be measured by DAPI staining in the range of 0.4–0.6 μg/mL to depict histograms with typical cell cycle phase distribution. Microscopic magnification during image acquisition, however, could distort the phase distribution. Exposure time did not significantly affect the cell cycle analysis. Furthermore, cell cycle inhibitor rapamycin treatment changed the cell cycle phase distribution as expected. In conclusion, a method for microfluidic single-cell cell cycle analysis of spread cells in situ was developed. Factors such as dye concentration and microscopic magnification had more influence on cell cycle phase distribution. Further studies will focus on detail differentiation of cell cycle phases and the application of such a method for biological meanings.Keywords:microfluidics;cell cycle;spread cell;DNA content
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Tianhan Zhang,Chengyi Xie,Yong Wu,Wenqing Gao,Chenlu Wang,Keqi Tang,Jiancheng Yu
A compact in-trap single photon ionization (SPI) system, termed discontinuous atmospheric pressure interface (DAPI) ion trap mass spectrometry (ITMS), for the rapid determination of trace volatile organic compounds (VOCs). DAPI enables the maintenance of high analyte pressure in the SPI source while preserving the vacuum. Utilizing vacuum ultraviolet (VUV) lamp as the ion source, we ionize the analyte within an enclosed miniature 3D ion trap for efficient analysis. Experimental findings demonstrate that the system achieves a mass resolution of less than 0.5 atomic mass units for full width at half maxima (FWHM), providing unit resolution. For the response for benzene, the system achieves almost instantaneous response, with rise and fall times of only 2 s, which is a significant advantage compared to the membrane inlet (MI) approach necessary for on-site detection. Additionally, tandem mass spectrometry (MS/MS) with unit m/z isolation window was achieved and resolved dimethyl disulfide and phenol with similar molecular weights. Our results demonstrate that the system provides excellent performance and we anticipate its application in rapidly determining VOCs.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Cancer Cell International
·
2021
·Vol. 21
·期 1
·1-12
·
IF 5.722
Jiang Dongxiao,Ding Shufei,Mao Zhujun,You Liyan,Ruan Yeping
Background Colon cancer is a malignant gastrointestinal tumour with high incidence, mortality and metastasis rates worldwide. Aloe-emodin is a monomer compound derived from hydroxyanthraquinone. Aloe-emodin produces a wide range of antitumour effects and is produced by rhubarb, aloe and other herbs. However, the mechanism by which aloe-emodin influences colon cancer is still unclear. We hope these findings will lead to the development of a new therapeutic strategy for the treatment of colon cancer in the clinic.Methods We identified the overlapping targets of aloe-emodin and colon cancer and performed protein–protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. In addition, we selected apoptosis pathways for experimental verification with cell viability, cell proliferation, caspase-3 activity, DAPI staining, cell cycle and western blotting analyses to evaluate the apoptotic effect of aloe-emodin on colon cancer cells.Results The MTT assay and cell colony formation assay showed that aloe-emodin inhibited cell proliferation. DAPI staining confirmed that aloe-emodin induced apoptosis. Aloe-emodin upregulated the protein level of Bax and decreased the expression of Bcl-2, which activates caspase-3 and caspase-9. Furthermore, the protein expression level of cytochrome C increased in a time-dependent manner in the cytoplasm but decreased in a time-dependent manner in the mitochondria.Conclusion These results indicate that aloe-emodin may induce the apoptosis of human colon cancer cells through mitochondria-related pathways.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Ao Li,Peng Zuo,Bang-Ce Ye
Salmonella, a typical foodborne pathogen, always seriously threatens the health and even life of both humans and animals. However, highly sensitive and fast quantitative methods for its detection are remaining to be challenged. Herein, we presented an efficient method with dual signal amplification strategy by combining immune hybridization chain reaction (HCR) with surface enhanced Raman scattering (SERS) to high sensitively detect Salmonella typhimurium in food. After sample preparation, S. typhimurium were specifically captured by immunomagnetic beads (IMBs), then aptamers and hairpin-probes were added to trigger HCR to form nicked dsDNA, finally 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was incubated with HCR products and then the whole system was mixed with AgNP colloid to detect the SERS intensity at 1610 cm −1 . As a result, a good linear relationship was achieved between SERS intensities and corresponding concentrations of S. typhimurium ranging from 10 to 10 5 CFU/mL, with a limit of detection (LOD) of 6 CFU/mL in 3.5 h. The proposed method has been successfully applied to capture and detect the S. typhimurium in spiked milk samples, and the results were consistent with those of the traditional plate counting method. The method, with combination of HCR and SERS, achieves double amplification of the detection signal and significantly improves the detection sensitivity of S. typhimurium . And it also shows good application potential for the highly sensitive detection of other contaminants in food.
研究领域:
食品科学
·
生物传感器
·
病原体检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
·
2017
·
IF 3.505
Jun Tian,Zhaoqun Lu,Yanzhen Wang,Man Zhang,Xueyan Wang,Xudong Tang,Xue Peng,Hong Zeng
The antifungal activity of Nerol (NEL) against Candida albicans , a pathogenic fungus, has a minimum inhibitory concentration (MIC) of 4.4 mM that causes noteworthy candidacidal activity through an apoptosis-like mechanism. Calcium (Ca 2+ ) levels and reactive oxygen species (ROS) production, which are the major causes of apoptosis, were determined in C. albicans cells treated with NEL and were found to increase, which related to mitochondrial dysfunction and disruption. A series of characteristic changes of apoptosis caused by NEL, including mitochondrial membrane depolarization, cytochrome c (cyt c) release, and metacaspase activation were examined using a flow cytometer and Western blot . The results showed that an increase in intracellular Ca 2+ and ROS led to dramatically decreased mitochondrial membrane potential (MMP); cyt c was also released from the mitochondria to the cytosol. Other early apoptotic features were also observed with the metacaspase activation. Finally, the morphological changes of the cells were observed, including phosphatidylserine (PS) externalization, nuclear condensation, and DNA fragmentation through Annexin V-FITC and PI double staining, TUNEL assay , and DAPI staining. The results supported the hypothesis that NEL was involved in the apoptosis of C. albicans cells not only at the early stages, but also at the late stages. In summary, NEL can trigger mitochondrial dysfunction and disruption via elevation of Ca 2+ and ROS leading to apoptosis in C. albicans . This research on the mechanism of cell death triggered by NEL against C. albicans has important significance for providing a novel treatment of C. albicans infections.
研究领域:
微生物学
·
药理学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jingbo Sun,Hongchen Li,Min Ouyang,Jing Cheng,Dong Xu,Xiaofeng Tan,Qinlu Lin
Cytoarchitectural staining is of great importance in disease diagnosis and cell biology research. This study developed user-friendly multifunctional red-emissive carbon dots (R-CDs) for rapid cell nucleus staining via targeting nuclear proteins. R-CDs, simply prepared by electrochemical treatment of 1,2,4-benzenetriamine, exhibit strong emission at 635 nm when excited at 507 nm. The R-CDs can rapidly stain the nucleus of human SH-SY5Y, HepG2, and HUH-7 cells with a high signal-to-noise ratio owing to fluorescence enhancement after entering the nucleus. Compared to conventional cytosolic dyes such as Hoechst and DAPI, R-CDs are cheaper, more highly dispersed in water, and more stable (requiring no stringent storage conditions). The R-CDs show stable optical properties with insignificant photobleaching over 7 days and salt resistance up to 2 M of NaCl. More importantly, R-CDs, possessing a positive charge, allow rapid staining of live cells (3 min) and dead cells (10 s) in saline. According to kinetic variation, R-CDs can distinguish live cells from dead cells. Staining exhibits high efficiency in onion epidermal cells, Aspergillus niger, Caenorhabditis elegans, and human spermatozoa. The mechanism for efficient staining is based on their fast accumulation in the nucleus due to their small size and positive charge and strong interaction with nuclear proteins at amino acid residues of histidine and arginine, resulting in fluorescence enhancement by dozens of times. The developed R-CDs do not bind to DNA and would not cause genetic damage and will find various safe applications in biological and medical fields.
研究领域:
细胞生物学
·
疾病诊断
·
材料科学
·
光学性质
·
生物医学应用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iScience
·
2025
·
IF 4.1
Yani Hui,Bing Li,Shan He,Haiyan Jiang,Yanlan Xie,Mingzhu Lv,Chuan Zhang,Xiaoxue Xu,Yuan Yong,Hanfeng Yang
Computed tomography (CT)-guided percutaneous microwave ablation (MWA) serves as a standard approach for hepatocellular carcinoma (HCC) treatment, yet faces challenges of higher recurrence rates when treating tumors in special anatomical locations. This study evaluated tungsten disulfide (WS 2 ) nanosheets combined with CT-guided MWA for treating rabbit VX2 liver tumors. WS 2 nanosheets were synthesized and demonstrated excellent microwave thermal effects and CT imaging capability in vitro. In animal studies, the combination therapy significantly enhanced tumor necrosis and apoptosis rates compared to MWA alone, while also activating systemic antitumor immunity as evidenced by increased Cytotoxic Tlymphocyte (CD8 + T-cell) infiltration. The findings suggest WS 2 nanosheets improve MWA efficacy through enhanced energy absorption and immune activation, offering a promising combinatorial strategy for hepatocellular carcinoma treatment.
研究领域:
肿瘤学
·
纳米医学
·
介入放射学
·
癌症治疗
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2025
·
IF 8.2
Sichan Li,Shunzi Shao,Zhaokun Zhang,Zhuo Wang,Xiaolei Han,Xiaoqian Jia,Hongxin Zhang,Yuan Gao,Xiaobo Zhang
Metabolic dysfunction-associated steatotic liver disease (MASLD) has become a global epidemic, leading to an unmet medical need for pharmacological interventions. This study aims to investigate the protective effect and mechanism of a synthetic wogonin derivate V8 against hepatic steatosis. Herein, we report that V8 attenuates lipid accumulation and prevents mitochondrial dysfunction in hepatocytes. Growth differentiation factor 15 (GDF15), a well-known cytokine with powerful metabolic action, mediates the effect of V8 on lipid metabolic disorders. Mechanistically, due to its lysosomotropic property, V8 promotes nuclear translocation of transcription factor EB (TFEB) and subsequent GDF15 gene transcription through activating adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK). Further analysis demonstrates GDF15 mainly improves mitochondrial homeostasis under stressful condition. Consistently, Gdf15 ablation in mice results in aggravated hepatic lipid deposition and liver injury upon high-fat diet feeding. Moreover, V8 alleviates diet-induced hepatic steatosis in a GDF15-dependent manner, and improves toxins-induced hepatic pathological phenotypes accompanying with upregulation of GDF15. Collectively, these data demonstrate the beneficial metabolic effect of V8 and highlight its utility in treating hepatic steatosis and its associated metabolic complications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Anesthesiology
·
2025
·
IF 2.6
Peng Pan,Sixv Ding,Wang Yang,Yibo Wang,Hua Qian,Ayang Zhao,Wengang Ding
Non steroidal anti-inflammatory drugs (NSAIDs) are widely used for analgesic and anti-inflammatory purposes but are often hampered by side effects such as gastrointestinal issues. Flurbiprofen, a potent NSAID, has significant therapeutic potential but may cause adverse effects due to its non-selective cyclooxygenase (COX) inhibition. This study aimed to enhance its efficacy and reduce side effects by developing flurbiprofen-loaded ZIF-8 nanoparticles (FP@ZIF-8). ZIF-8, with its high porosity, pH sensitivity, and biocompatibility, serves as an excellent carrier for targeted drug delivery. FP@ZIF-8 nanoparticles demonstrated successful flurbiprofen encapsulation with a drug loading rate of up to 53% and showed pH-responsive drug release. In vitro studies revealed that FP@ZIF-8 nanoparticles not only promoted the proliferation of human umbilical vein endothelial cells (HUVECs) but also significantly reduced lipopolysaccharide (LPS) -induced reactive oxygen species (ROS) production in RAW264.7 macrophages. In vivo experiments further indicated that FP@ZIF-8 effectively alleviated inflammation-induced pain in mouse models and downregulated key inflammatory cytokines. The biosafety assessment confirmed the excellent biocompatibility and safety of FP@ZIF-8 nanoparticles. These findings suggest that FP@ZIF-8 is a promising drug delivery system for treating inflammatory pain conditions.
研究领域:
纳米医学
·
药学
·
炎症性疾病
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Abdullrahman M. Al-Bishari,Yan Wang,Anni Wu,Haobo Sun,Bilal A. Al-Shaaobi,Amal M. Jama,Keqian Zhu,Xiaowei Lou,Mohammed S. Abdelmajeed,Ahmed S. Mahany,Lifeng Xiong,Ningyao Sun,Mingliang Yu,Zhennan Deng,Lei Lu,Jinsong Liu,Peng Gao
Dental implants face challenges such as poor osseointegration, delayed angiogenesis, and chronic inflammation. Endogenous gaseous signaling molecules hydrogen sulfide (H 2 S) and nitric oxide (NO) have emerged as key gasotransmitters that regulate angiogenesis, osteogenic differentiation and immune responses, making them attractive candidates for microenvironment-targeted therapies. Here, we engineer a dual gasotransmitter-releasing coating by co-electrospinning hydrogen sulfide (H 2 S) and nitric oxide (NO) donors, JK-2 and S-Nitroso- N -acetyl-DL-penicillamine (SNAP), into gelatin/polycaprolactone (Gel/PCL) nanofibers (NFs). These NFs were applied to laser-microgrooved titanium (Ti) surfaces, further modified with polyepigallocatechin gallate (pEGCG) to ensure stable attachment. The resulting coating exhibited sustained, pH-responsive release of H 2 S and NO, which acted synergistically to enhance cellular responses. In vitro , the dual gasotransmitter-releasing NFs coating enhanced endothelial cell migration, tube formation, and upregulated angiogenic markers (VEGF, TGF-β1, eNOS). Additionally, MC3T3 preosteoblasts showed increased alkaline phosphatase (ALP) activity, mineralization, and upregulation of osteogenic genes (Runx2, COL1, OPN, OPG). RNA-sequencing identified enriched pathways related to histone methylation and oxidative phosphorylation, crucial for osteogenesis and cellular metabolism. Macrophages cultured with these NFs demonstrated suppressed M1 pro-inflammatory markers and enhanced M2 polarization, indicating a shift toward a more regenerative immune response. In vivo , the NFs coating significantly improved neovascularization, bone volume, collagen deposition, and peri-implant tissue thickness. This synergistic release of H 2 S and NO provides a promising strategy to enhance osseointegration and bone regeneration by modulating angiogenesis and immune responses.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）