Dandan Fang,Mian Pan,Huan Yi,Hong Dai,Zhensheng Hong,Xiangqin Zheng,Yanyu Lin
Herein, an enhanced lectrochemiluminescence (ECL) immunosensor was designed based on an efficient ECL bioprobe and first realized the quantitative analysis of human epididymis protein 4 (HE4). To obtain the efficient ECL bioprobe, a self-assembled mesocrystalline hybrid (TiO 2 MCs@rGO) was first prepared for loading luminol and 2-(Dibutylamino)ethanol (DBAE), which was explored as a new coreacant of luminol for the first time and triggered more stable and efficient ECL intensity than conventional luminol-H 2 O 2 system. Additionally, the high surface area and outstanding conductivity of TiO 2 MCs@rGO would effectively amplify the ECL intensity. Besides, the synthesized macromolecular complex (PEDOT/PSS/NiCo 2 O 4 NS) integrated the excellent conductivity of PEDOT/PSS and highly porous of NiCo 2 O 4 nanosheets was served as an efficient sensing matrix for further improvement of the analysis sensitivity. The developed ECL immunosensor exhibited excellent performance in the analysis of HE4 with a wide linear range of 1.0 × 10 −5 ˜1000 ng/mL and low detection limit of 3.3 × 10 −6 ng/mL, especially superior stability. The strategy provided promising application for early ovarian cancer monitor and diagnosis, and provoked a new idea for other tumor markers detection.
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
Yi Xiao,Suhua Chen,Simeng Zhang,Guofang Wang,Haomin Yi,Gui-Zhong Xin,Xiaoping Yang
Straightforward and accurate measurement of medical biomarkers is of essential importance in clinical diagnostics and treatments. However, the major challenge is the diversity in dynamic range of different biomarkers ranging from pg mL −1 to μg mL −1 in various body fluids and tissues among patients. Here, we develop a mesoporous silica (MS)-mediated controllable electrochemiluminescence (ECL) quenching of immunosensor that allows accurate immunoassays with simplicity, sensitivity and tunable sensing range. MS is employed to enhance the sensitivity and tune ECL quenching to broaden the detection range just by altering luminophore (Ru(bpy) 3 2+ ) and coreactant (DBAE) concentration without additional modifications. The immunoassay is followed: homogeneous sandwich immunoreaction, magnetic separation, and ECL quenching detection. As a proof-of-concept, simple and sensitive detection of IgG is achieved ranging from pg mL −1 to μg mL −1 , and applications of the strategy are extended by the combination of ECL immunosensor with commercial ELISA kit. This study will not only be expected to serve as a new avenue for the assay of physiological and clinical implications of immunological biomarkers, but also benefit a wide range of applications that require a tunable detection range and ultrahigh sensitivity.
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