INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Jun Yuan,Shuxian Chen,Liping Wang,Tingting Xu,Xu Shi,Yi Jing,Haijiang Zhang,Yange Huang,Ying Xu,Dong Li,Xing Chen,Jianhui Chen,Qingping Xiong
The aim of this paper was to prepare purified fractions of polysaccharides from Monetaria moneta Linnaeus and further compare their characteristics and antioxidant activities. Firstly, three novel purified fractions, named MM-P1, MM-P2 and MM-P3, were successfully prepared by a DEAE-Sepharose fast-flow column. Then, their characteristics were compared using chemical testing, FT-IR, GC and HPGPC. The results suggested that MM-P3 had higher molecular weights than MM-P1 and MM-P2. MM-P1 was consisted of glucose, MM-P2 was consisted of glucose and xylose, and MM-P3 was comprised of glucose, xylose and mannose. Differed from MM-P1 and MM-P2, MM-P3 had sulfuric radical and uronic acid groups. Finally, their antioxidant activities were also compared. We found that MM-P3 exhibited better antioxidant bioactivities than MM-P1 and MM-P2. The data demonstrated that three purified fractions derived from different adsorption capacity of DEAE-Sepharose fast-flow column possessed different structural characteristics and antioxidant activity.
研究领域:
生物化学
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药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2016
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IF 4.219
Fangfang Xu,Kangsheng Liao,Yunshan Wu,Qi Pan,Lilan Wu,Hong Jiao,Dean Guo,Ben Li,Bo Liu
Extraction optimization, purification, characterization, sulfation and antitumor activity of polysaccharides from the fruit body of Borojoa sorbilis cuter were investigated in present study. The optimal Ultrahigh Pressure extraction condition was determined as: extraction once with the solid-liquid ratio of 1:10 in 30 °C and 1500 Mpa for crude polysaccharide (BP) and experimental yield was 8.28%. Four water-soluble polysaccharides named as BP1-1, BP1-2, BP1-3 and BP1-4, with molecular weight of 35.8, 32.4, 30.1 and 27.7 kDa, were purified by DEAE Sepharose and Superdex 200 chromatography. On the basis of chemical and spectroscopic analyses, BP1-1–BP1-4 were found to be neutral β- d -galactan containing a (1 → 4)-linked backbone. S-BP1s with the DSS of 1.18, was sulfated by chloro-sulfonic acid-pyridine method. Furthermore, S-BP1s exhibited significant in vitro antitumor activity against liver cancer HepG2 and lung cancer A549 cells in a dose-dependent manner. The results indicated that S-BP1s could be potentially developed as functional antitumor drug.
研究领域:
药理学
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生物化学
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分子生物学
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癌症研究
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DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF MICROBIOLOGY
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2022
·Vol. 54
·期 1
·199-211
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IF 2.214
Chen Guoqiang,Xu Zhenxiang,Wang Fan,Liu Lijuan,Wei Yuxi,Li Jinglong,Zhang Liang,Zheng Kai,Wu Lijun,Men Xiao,Zhang Haibo
The exploration of polysaccharides from microorganisms is of great importance. In this study, a new type of exopolysaccharide excreted by Fusarium merismoides A6 (FM-EPS) was isolated, and the extraction conditions were optimized using a response surface methodology (RSM). The extraction temperature at 0 °C, a precipitation time of 7.83 h, and an ethanol precipitation concentration of 77.64% were predicted and proved to be the best extraction conditions with the maximum extraction yield of 0.74 g/mL. Then, two fractions of F . merismoides A6 exopolysaccharides (FM-EPS1 and FM-EPS2) were obtained through DEAE Sepharose fast flow column chromatography. As indicated by monosaccharide composition analysis, both fractions mainly consisted of mannose, glucose, galactose, and ribose, with an average molecular weight of 5.14 × 10 4 and 6.50 × 10 4 g/mol, respectively. FT-IR and NMR spectroscopy indicated the FM-EPSs had both α- and β-glycosidic bonds. Moreover, the determination of antioxidant and antiproliferative activities in vitro proved that FM-EPSs had good antioxidant activities and antiproliferation activities. FM-EPS1 showed stronger antioxidant activities than FM-EPS2. FM-EPS2 showed antiproliferation activities on HeLa and HepG2 cells, while FM-EPS1 had no obvious antiproliferative activity. Therefore, FM-EPSs could be explored as potential antioxidant and anticancer agent applied in food, feed, nutraceutical, pharmaceutical, cosmetics, and chemical industries.
研究领域:
微生物学
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生物化学
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药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2019
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IF 3.197
Ping-Ping Wang,Qiang Huang,Chun Chen,Li-Jun You,Rui Hai Liu,Zhi-Gang Luo,Mou-Ming Zhao,Xiong Fu
A novel polysaccharide (MFP4P) was isolated from mulberry fruits ( Morus alba L . ) pulp using a combination of DEAE-Sepharose fast-flow and Sephadex G-100 chromatographic columns. Structural characterization revealed that MFP4P was composed of arabinose, rhamnose, galactose, glucose and galacturonic acid with a molecular weight of 198.2 kDa. The linkage types of MFP4P were proved to be (1 → 6)-linked α-D-Glc, (1 → 2)-linked α-L-Rha, (1 → 3)-linked α-D-Gal, (1 → 3)-linked β-L-Rha and (1 → )-linked α-L-Ara. The MFP4P showed good antioxidant activity with an oxygen radical absorbance capacity value of 121.8 ± 16.5 μM trolox equivalent/g. In addition, the MFP4P promoted pancreatic cell proliferation, increased insulin secretion, and enhanced glucose consumption. In the non-toxic range, the polysaccharide and zinc complexes (MFP4P-Zn) exhibited higher antioxidant and hypoglycemic activities than MFP4P at the same concentration. In vivo hypoglycemic study, MFP4P and MFP4P-Zn both showed decreased blood glucose level and glucose tolerance compared with the model control group.
研究领域:
药理学
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生物化学
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营养科学
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分子生物学
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产品关联
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
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2019
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IF 3.775
Yongyi Yu,Yajun Zhang,Cebin Hu,Xinyuan Zou,Yang Lin,Yangyuan Xia,Lijun You
An immunologically active polysaccharide named as UPP-2 (1035.52 kDa) was isolated from Undaria pinnatifida using traditional water extraction followed by DEAE Sepharose fast flow chromatography. UPP-2 was proven to be a low sulfated polysaccharide with relatively abundant uronic acid (13.08 ± 0.67%). UPP-2 mainly consisted of xylose (64.55%), glucose (23.81%), arabinose (5.90%) and mannose (4.26%), and its main glycosidic linkage types included →2)- α -D-Xylp-(1→, →4)- α -D-Glcp-(1→, α -D-Xylp-(1→ and →2,4)- β -D-Xylp-(1 → . Results indicated that UPP-2 significantly promoted the proliferation and pinocytic capacity of RAW264.7 cells, and upregulated the mRNA expressions of iNOS, TNF- α , IL-6 and IL-1 β at 100–600 μg/mL with a maximum of 195, 42, 768 and 539 times of those of the negative control, respectively. Moreover, UPP-2 significantly increased the secretions of nitric oxide , TNF- α and IL-6 at 100–600 μg/mL (8.0, 73.1 and 188.7 times compared to those of the negative control, respectively), as well as promoted the production of IL-1 β obviously at 600 μg/mL. Overall, UPP-2 could be served as a potential dietary supplement or functional food based on its immunostimulatory activity.
研究领域:
海洋生物学
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免疫学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）