In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using petroleum ether/ethyl acetate/ acetone/glacial acetic acid (29:1:0.5:2d*, v/v/v/v) as the developing solvent and bromine thymol blue solution as the chromogenic agent. The content of DEHA in freeze-dried Antarctic krill was found to be ca. 0.63 ± 0.05 mg/g. The structure of DEHA in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC-MS) and infrared chromatography, which verified the presence of this compound in the krill. The HPTLC method exhibited excellent accuracy, with a recovery of 97.1–101.6% and good precision with a relative standard deviation of 2.47–4.90%. The DEHA in Antarctic krill oil was extracted by n-hexane and detected using the same method described above, which verified that DEHA was also present in krill oil at a concentration of ca. 2.16 ± 0.08 mg/g. The presence of DEHA in kill oil is very concerning because of its demonstrated harmful ecotoxicity, and since Antarctic krill is the key link in the food chain in the Antarctic coastal marine ecosystem. The adverse effects of DEHA on Antarctic krill and the source of DEHA will be explored in future research.
The health risks of exposure to plasticizers have received widespread attention, however, little is known about the effects of fast food packaging plasticizers on steroid hormone synthesis. In the present study, the types and migration of plasticizers in some commonly used fast-food packaging materials were detected by GC–MS, and the interference effects of these plasticizers and their metabolites on steroid hormone synthesis in the human body were evaluated by the H295R steroidogenesis assay. The GC–MS results showed that the main plasticizer compounds that migrated from fast food packaging into food were di(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) adipate (DEHA). Exposure to these chemicals (100–1000 μM) can significantly reduce the viability of H295R cells in a dose-response manner, and these plasticizers and their metabolites that migrated into oily foods at high temperatures (0.25–25 μM) could significantly increase the E2 level and reduce the T level in H295R cells. According to the qRT-PCR data, 0.25 to 25 μM mono(2-ethylhexyl) phthalate (MEHP) significantly upregulated the expression levels of 17β-HSD1 and CYP19A1, and downregulated those of CYP17A1, CYP11A1 and StAR. The Western blot results were consistent with those of qRT-PCR. In summary, these results indicated that even exposure to low concentrations (≤1 mg/l or 2.5 μM) of these chemicals and their metabolites can cause significant endocrine-disrupting effects.
危险品化学品经营许可证（带存储）