JOURNAL OF BIOTECHNOLOGY
·
2025
·
IF 3.9
Lan Yao,Zhe Zhang,Guangyu Chen,Zhiyuan Sun,Xiong Chen,Haitao Yang
The enzymatic hydrolysis of lignocellulose continues to be encumbered by elevated production costs and diminished cellulase efficiency. In this work, modified DES recovered lignin was obtained by grafting acrylamide and acryloyl chloride to enhance glucose release. At a cellulase dosage of 5 FPU/g-cellulose and pH of 5.5, modified lignin promoted glucose yield of dilute-acid-pretreated wheat straw by 158% compared with control. The mechanism by which modified lignin promotes enzymatic hydrolysis was further explored. The binding constant was reduced from (3.3510±0.8361)*10 4 to (2.7600±0.6027)*10 3 L•mol -1 after modification. Modified lignin could make α-helix content enhancement so that cellulase had a compact and stable spatial structure. Lignin binds within the catalytic tunnel of cellulase and that the modified lignin interacts with cellulase with increased hydrogen bonding, resulting in a more compact cellulase structure. The modified lignin might reduce the unproductive adsorption of cellulase, and increase stability and cellulose accessibility to reduce cellulase cost.
研究领域:
生物化学
·
生物质能
·
酶工程
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 15
·4284-4292
·
IF 7.59
Ruixia Liu,Tingting Ding,Pingping Deng,Xiaofan Yan,Fuquan Xiong,Jienan Chen,Zhiping Wu
This work was carried out in order to broaden the application field of lignin and improve its additional value. The degraded deep eutectic solvent lignin-grafted poly(N-vinyl caprolactam) (DES-lignin-g-PNVCL) was synthesized by using modified DES-lignin and NVCL via activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP). Aspirin was coated with DES-lignin-g-PNVCL through self-assembly by an ethanol/water anti-solvent method to obtain lignin thermosensitive polymer nanoparticle coated aspirin (aspirin@LTNP). X-ray electron spectroscopy (XPS), elemental analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and ultraviolet visible spectroscopy (UV) were used to characterize the composition, structure and morphology of DES-lignin-g-PNVCL and aspirin@LTNP. The releasing behavior of aspirin@LTNP at different temperatures and pH values was investigated. The safety was evaluated by cytotoxicity tests. The results indicated that aspirin@LTNP was mainly accumulated by the hydrophobic effect and π–π interaction in the process of self-assembly, and its morphology was an ellipsoid stacked layer by layer. The aspirin@LTNP hydrophilic chains were increased and had externally hydrophilic and internally hydrophobic structures. The particle size decreased slightly during the self-assembly process. The red-shift occurred at the π–π interaction wavelength of the lignin aromatic ring, which indicated a physical coating process. The coating rate of aspirin@LTNP was 88.87%. Aspirin@LTNP showed an obvious temperature response; the 96 h cumulative release rate at the LCST was 73.75 ± 1.16%, while the 96 h cumulative release rate above the LCST was 28.10 ± 0.92%. The 96 h cumulative release rate was 63.21 ± 0.57% at pH = 1.5 and 49.56 ± 0.48% at pH = 7.4. The dosage of aspirin@LTNP used in the experiment was safe. This study provided a strategy for drug coating and controlled release.
研究领域:
材料科学
·
药学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 6
·849-858
·
IF 3.1
Dandan Li,Xinxing Jiang,Yaxue Zhang,Wenxia Xue,Jihong Fu
A novel approach based on polydimethylsiloxane/graphene oxide/deep eutectic solvent (PDMS/GO/DES) sponge headspace solid phase extraction followed by GC-MS was successfully developed to determine the volatile components in cumin. The PDMS/GO/DES exhibits outstanding properties with high adsorption capacity and good chemical stability, and has shown its potentiality as an ideal adsorbent for the extraction of volatile compounds. The influence factors of the extraction process were investigated. Excellent analytical performances were achieved, including wide linearity (0.60–107.72 ng) with high correlation coefficients (R2 ≥ 0.9951), low LODs (0.23–9.23 ng) and LOQs (0.54–18.47 ng), satisfactory precision (intra-day RSDs ≤ 2.85% and inter-day RSDs ≤ 3.92%). Under the optimal extraction conditions, the volatile components in 17 cumin samples from four origins in Xinjiang were analyzed and 31 compounds were identified. PCA was used to establish the relationship between the origins and the volatile compounds for further discriminant analysis. The results showed that the PDMS/GO/DES method was a rapid, simple and sensitive technique for the analysis of volatile components in spices.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2021
·Vol. 67
·期 8
·e17252
·
IF 3.993
Hu Li,Zisheng Zhang,Guanlun Sun,Suli Liu,Liangcheng An,Xingang Li,Hong Li,Xin Gao
As an attractive alternative technology for the separation of long chain olefin and paraffin, a novel silver-based deep eutectic solvent (Ag-DES) was prepared and utilized for 1-octene/n-octane separations. Comprehensive reactive extraction separation experiments were performed to highlight the Ag-DES concentration and operating temperature discriminations using compounds with different ratio of 1-octene/n-octane. The novel Ag-DES showed optimal separation performance regarding 1-octene/n-octane and possessed the highest levels separation selectivity in the range 3.57–16.11 with excellent circulation stability in our best knowledge. Furthermore, FT-Raman measurements and quantum chemistry calculation were performed to elucidate the interaction mechanism of Ag-DES in the separation of 1-octene and n-octane, which revealed that both chemical complexation and strong physical attraction existed in the complex of Ag-DES and 1-octene rather than n-octane. A practical process was proposed for the separation of olefin and paraffin, which indicates that an advanced separation technology could largely reduce the energy consumption. This study lends important insight for the development of Ag-DES reactive extraction separation process for the energy-efficient long chain α-olefins purification from F-T synthesis products.
研究领域:
化学工程
·
能源科学
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2019
·Vol. 21
·期 5
·1075-1079
·
IF 9.405
Yong Ni,Zhihao Bi,Hui Su,Lifeng Yan
Efficient conversion of furfural to valuable chemicals is attracting attention because it is one of the bulk feedstocks in biomass refinery. Maleic acid (MA) and fumaric acid (FA) are two types of bulk monomers that can be prepared from furfural. Here, a simple method was developed to oxidize furfural to both MA and FA in a green deep eutectic solvent (DES). DES contained oxalic acid and choline chloride (ChCl); oxalic acid worked as both the main content of the solvent and acidic catalyst for the conversion. It was found that the conversion of furfural could reach 100%, while the yield of MA and FA reached 95.7% under a mild reaction temperature of 50 °C, which provided a green and efficient route for the synthesis of valuable monomers from furfural.
研究领域:
化学工程
·
绿色化学
·
有机合成
·
生物质转化
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Yuanbin Li,Wu Yi,Haowei Chen,Bao Han,Bo Ruan,Zhong Xiaoyu,Li ShanShan,Yifan Chen,Tao Peng,Jiaheng Zhang,Fang Wang
Betaine/ascorbyl glucoside (Bet/AA-2G) deep eutectic solvents (DES) composed of betaine and ascorbyl glucoside had been successfully used to improve the solubility of silymarin in this study. Under appropriate conditions, the solubility of silymarin in a 5 wt% Bet/AA-2G DES solution increased by 17.7% and 60.9% compared to a 50 wt% 1,3-butanediol solution and a 50 wt% ethanol solution, respectively. In addition, quantum chemical simulations showed that silymarin has a strong binding capacity to Bet/AA-2G DES and can remain stable after binding. The combination of Bet/AA-2G DES with silymarin enhanced the skin permeability and antioxidant capacity of silymarin. Moreover, SM-DES promoted the synthesis of type I collagen and significantly reduced the expression of MMP-1, indicating potential anti-wrinkle and firming properties. Overall, Bet/AA-2G DES showed great potential for improving the solubility and stabilizing the bioactivity of silymarin.
研究领域:
药学
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化学
·
化妆品科学
·
材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Yiwen Liu,Ruirui Liu,Qingliang Yang,Gensheng Yang
As a natural small molecule drug derived from turmeric, curcumin is known for its good biosafety and a range of beneficial effects, including anti-inflammatory, anti-spasmodic, antioxidant, antibacterial, anti-tumor, and neuroprotective effects. Even though, its insolubility in water and instability severely limit its bioavailability and clinical applications. The present study developed a deep eutectic solvent (DES) in oil microemulsion (DES/O-ME) system loaded with Cur for intranasal administration, aiming to enhance both the solubility and permeability of Cur to significantly increase its bioavailability. The project first constructed and screened the optimal choline chloride-based DES. Subsequently, the DES/O-ME system was prepared and optimized to achieve uniform particle size and stability using a pseudo-ternary phase diagram and electrical conductivity measurements. The resulting DES/O-ME system was thoroughly evaluated for its solubilization efficacy, permeability, mucosal cytotoxicity, and stability. Additionally, the therapeutic efficacy of the Cur-DES/O-ME was assessed in vivo using a murine model of allergic asthma. This comprehensive evaluation highlights the potential of the DES/O-ME system as a promising intranasal drug delivery platform to overcome the limitations of curcumin’s bioavailability and clinical application.
研究领域:
药学
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药物递送系统
·
纳米技术
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药理学
·
呼吸系统疾病
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Junlei Wang,Zhimin Zhao,Hanmeng Yuan,Hongshuai Gao,Yi Nie
Keratin, a naturally occurring biomaterial, has gained substantial recognition for its application in various domains. Given its abundant presence in human hair, the pursuit of efficient methods for dissolving and extracting keratin is motivated by the dual objectives of maximizing the utility of human hair and promoting ecological sustainability. In this work, a series of deep eutectic solvents (DES) were designed for extraction of keratin from human hair under mild experimental conditions. The effects of DES structure, molar ratios of HBA to HBD, dissolution temperature, and time on the dissolving ability of DES for human hair were investigated in depth. The results showed that ChCl/MEA can effectively dissolve human hair under mild conditions (80 °C, 4 h) with a solubility of 84% and a mass percentage of 20%. FT-IR, XRD, SDS-PAGE, and TGA results showed that the structure of the regenerated keratin was slightly destroyed but still exhibited stable thermal property. Analysis of the reduced density gradient (RDG) and surface electrostatic potential (ESP) verified that the hydrogen bond driven by electrostatic attraction is formed in DESs. Independent gradient model (IGMH) analysis confirmed that the weak interaction between human hair and DES is mainly caused by van der Waals interactions. Additionally, ChCl/MEA displayed an excellent performance in extracting keratin from human hair even after recycling for three cycles.
研究领域:
材料科学
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化学工程
·
生物技术
·
可持续化学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Pei-Ru Zhou,Yu Chai,Jia-Tong Chen,Xiao-Ping Zheng,Yan-Zhen Zheng
The valorization of agricultural waste is critical for sustainable development, yet challenges persist in obtaining light-colored lignin with preserved structural integrity. In this study, an innovative one-step pretreatment strategy integrating deep eutectic solvents (DESs) with H 2 O 2 was developed to extract light-colored lignin directly from tea stem waste. The DES-H 2 O 2 system enabled the selective dissociation of lignin–carbohydrate complexes while preserving the fundamental aromatic structure of lignin. By varying the H 2 O 2 dosage, significant improvements in lignin decolorization were achieved, as evidenced by a marked reduction in the color difference relative to milled wood lignin (MWL). Comprehensive characterization using FTIR, GPC, Zeta potential, 31 P NMR, 2D-HSQC NMR, and XPS revealed that H 2 O 2 -induced oxidation not only facilitated the conversion of non-conjugated carbonyls into conjugated structures but also induced molecular depolymerization, reduced particle size and hydrogen-bond, and enhanced surface electronegativity. These modifications contributed to decreased chromophore efficiency and suppressed aggregation, ultimately yielding lignin with improved optical properties. Furthermore, incorporation of the light-colored lignin into a commercially available base cream demonstrated its potential as a sustainable UV-shielding agent, achieving a sun protection factor (SPF) exceeding 50 at optimal lignin concentrations. This work provides a promising green pathway for the high-value utilization of agricultural waste and lays the groundwork for the development of environmentally friendly, high-performance natural UV-protection products.
研究领域:
可持续化学
·
绿色技术
·
生物炼制
·
高分子科学
·
化妆品科学
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产品关联
DOI
高影响因子
Journal of Bioresources and Bioproducts
·
2021
·
IF 0
Jingwen Shao,Yong Ni,Lifeng Yan
Furfural is an alternative feedstock and has been used for the production of maleic acid (MA) and fumaric acid (FA) by an oxidation process. Deep eutectic solvents (DESs) were used as the green solvents while sodium chlorate was used as an oxidant and vanadium pentoxide was used as the catalyst at 70–90 °C under atmospheric pressure. It was found that several acidic DESs are valid, such as acetic acid/choline chloride (AA/ChCl) and propionic acid/choline chloride (PA/ChCl), and AA/ChCl DES was selected as the solvent for the conversion. The optimal DES is AA/ChCl, and the effect of the amount of oxidant, time, and temperature on the yield of the MA and FA has been systematically studied, and the conversion of furfural can reach 100% while the yield of the MA and FA reached 66.5% under reaction temperature of 80 °C for 12 h, which provides a new green route to synthesis valuable monomers from furfural.
研究领域:
化学工程
·
绿色化学
·
有机合成
·
催化
✓ 已验证
DOI
Small
·
2016
·Vol. 13
·期 3
·1602494
·
IF 8.315
Yu Zhang,Yifei Lu,Feng Wang,Sai An,Yujie Zhang,Tao Sun,Jianhua Zhu,Chen Jiang
Inflammation has been reported as one significant hallmark of breast cancer in relation to tumor development, metastasis, and invasion. The bradykinin receptor 1 (B1R) is highly expressed on inflammatory breast tumor cells thus providing a promising targeting site for tumor recognition and sufficient receptor mediated endocytosis. In this study, the authors evaluate the targeting efficiency of l -form and d -form [des-Arg 10 ]kallidin both in vitro and in vivo. To further improve the drug delivery efficiency, the authors establish a dandelion like nanoparticle by combining the polymeric drug conjugates and aptamer complex together. The doxorubicin conjugated polymer is complexed with adenosine-5′-triphosphate (ATP) sensitive hybridized aptamer in self-assembly process by intercalating into the double strand scaffolds. The acid labile conjugating bond and ATP sensitive aptamer endow the nanoparticle with dual responsiveness to intracellular milieu, thus triggering a quick drug release in tumor cells. Remarkable therapeutic effects and tuned in vivo pharmacokinetics profiles are shown by the aptamer complexed drug conjugates nanoparticle with B1R active targeting modification. Therefore the strategies of B1R targeting and ATP/pH dual-responsiveness nanoparticle help achieve enhanced drug accumulation within tumor cells and efficient chemotherapy for breast cancer.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yin Yan,Changliu He,Lihe Zhang,Huifeng Dong,Xu Zhang
In this paper, ternary DES (choline chloride, glycerol, Lewis acid) was used to pretreat lignocellulose, and the DES solution with dissolved lignin was utilized as the medium of hydrogel to prepare DES-based polyacrylic acid/polyvinyl alcohol (PAA/PVA) double network hydrogels with great mechanical properties, self-adhesion, and high electrochemical sensitivity. The entanglement of PAA with PVA chains, the covalent linkage between Al 3+ and PAA chains and the metal phenol network (MPN) formed by Al 3+ and lignin improved the mechanical properties of the hydrogels, enabling the prepared hydrogels to achieve a tensile strain of 400 % and an elongation at break of 150 kPa. Secondly, the introduction of DES solution endowed the hydrogel with excellent electrical sensing ability and anti-freezing property, so that the hydrogel still maintains good flexibility and ionic conductivity at −20 °C. It was also found that the above hydrogel can achieve a high gauge factor of 4.19 as a flexible sensor, which provides scientific ideas for the application of the pretreated DES solution in the field of flexible wearable.
研究领域:
材料科学
·
化学工程
·
生物医学工程
·
高分子科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）