Polymer Chemistry
·
2014
·Vol. 5
·期 24
·7109-7120
·
IF 5.368
Ning Che,Saina Yang,Hongliang Kang,Ruigang Liu,Zhuang Li,Zhijing Liu,Pingping Li,Xiaozhong Qu,Yong Huang
Dextran graft poly((N-amidino)hexyl methacrylamide) (Dex-g-PAHMA) copolymers were synthesized by free radical polymerization in aqueous solution and characterized. Dex-g-PAHMA copolymers can self-assemble into micelles with the PAHMA rich core and dextran rich shell in aqueous media. The CO2 sensitivity of the micelles was investigated by dynamic light scattering (DLS), conductivity and zeta potential. The results confirmed that the Dex-g-PAHMA copolymer micelles have reversible CO2 sensitivity. The micelles can be used as doxorubicin (DOX) carriers and DOX molecules are mainly located in the core of the micelles. The MTT assay indicated that the Dex-g-PAHMA graft copolymers are non-toxic to cells in the copolymer concentration range of 5–1000 μg mL−1, whereas the relative cell viability is greatly reduced with the increase of copolymer concentration for DOX-loaded micelles. The DOX-loaded micelles can be endocytosed by MCF-7 cells and the DOX can release from micelles and diffuse into the cell nucleus. The Dex-g-PAHMA copolymers have promising applications as drug carriers for cancer therapy.
研究领域:
聚合物化学
·
材料科学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 20
·3697-3705
·
IF 4.543
Changjiao Yan,Tiehong Yang,Sikai Zhu,Hong Wu
Immediate hemorrhage control without secondary injury is pivotal for saving lives. In this study, polymerized glycidyl methacrylate derivative dextran/acrylic acid (poly(DEX-GMA/AAc)) microgel particles were prepared via emulsion polymerization method as a hemostatic agent. Microgel particles with size distribution of 500–800 nm were chosen because they showed more appropriate characteristics of swelling ratio and gelation time. The results revealed that the microgel particles had excellent swelling ratio of 68.95 g g−1 (w/w), which was 8.4 times that of counterpart clinically used microporous polysaccharide hemospheres, Arista. And poly(DEX-GMA/AAc) showed very short gelation time of 10–13 s. As a result, a gelled film could be formed rapidly after poly(DEX-GMA/AAc) absorbed water in blood when used on wounds, and then staunched bleeding. Poly(DEX-GMA/AAc) microgel particles showed better clotting ability than commercial hemostatic agent Flashclot in vitro. In addition, poly(DEX-GMA/AAc) did not cause exothermic burn when absorbing liquid, which was superior to Flashclot. No obvious toxicity was found in cytotoxicity study and skin irritancy test. Blood loss and hemostasis time were dramatically reduced by poly(DEX-GMA/AAc) microgel particles in hemorrhage models of ear vein, ear artery, liver and femoral artery in rabbits. These results indicated that the poly(DEX-GMA/AAc) microgel particles are a potential hemostatic agent with almost no cytotoxicity and good biocompatibility.
研究领域:
医学材料学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Lingling Chen,Yang Zhang,Renjian Li,Haoquan Zhang,Meng Zhang,Hongwei Zhang
Membrane fouling restricts the wide applications of membrane technology and therefore, it is essential to develop novel analytical techniques to characterize membrane fouling and to further understand the mechanism behind it. In this work, we demonstrate a capability of high-resolution large-scale 3D visualization and quantification of the foulants on/in membranes during fouling process based on light sheet fluorescence microscopy as a noninvasive reproducible optical approach. The adsorption processes of dextran (DEX) on/in two polyvinylidene fluoride membranes with similar pore structure but distinct surface hydrophilicity were clearly observed. For a hydrophilic polyvinylidene fluoride (PVDF) membrane, the diffusion and adsorption of the DEX in membrane matrix were much slower compared to that for a hydrophobic membrane. A concentrated foulant layer was observed in the superficial potion of the hydrophilic membrane matrix while the foulants were observed quickly penetrating across the overall hydrophobic PVDF membrane during a short adsorption process. Both the inner concentrated fouling layer (in membrane superficial portion) and the foulant penetration (in membrane asymmetric structure) presented correlations with membrane fouling irreversibility, which could elucidate the microscopic events of hydrophilic membrane in resisting fouling. In addition, the imaging results could be correlated with the XDLVO analysis, suggesting how the membrane-foulant and foulant-foulant interfacial interactions resulted in a time-dependent membrane fouling process. This work provides a fast, highly-sensitive and noninvasive imaging platform for in situ characterization of membrane fouling evolution and should be useful for a wide range of membrane-based process explorations.
研究领域:
环境科学
·
生物工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2019
·
IF 4.396
Yina Song,Yanan Shi,Liping Zhang,Haiyan Hu,Chunyan Zhang,Miaomiao Yin,Liuxiang Chu,Xiuju Yan,Mingyu Zhao,Xuemei Zhang,Hongjie Mu,Kaoxiang Sun
The oral absorption of exenatide, a drug for type 2 diabetes treatment, can be improved by using nanoparticles (NPs) for its delivery. To improve the mucus penetration and intestinal absorption of exenatide, we designed a block copolymer, CSKSSDYQC-dextran-poly(lactic-co-glycolic acid) (CSK-DEX-PLGA), and used it for the preparation of exenatide-loaded NPs. The functionalized exenatide-loaded NPs composed of CSK-DEX-PLGA were able to target intestinal epithelial cells and reduce the mucus-blocking effect of the intestine. Moreover, the CSK modification of DEX-PLGA was found to significantly promote the absorption efficiency of NPs in the small intestine based on in vitro ligation of the intestinal rings and an examination of different intestinal absorption sites. Compared to DEX-PLGA-NPs (DPs), the absorption of CSK-DEX-PLGA-NPs (CDPs) was increased in the villi, allowing the drug to act on gobletlike Caco-2 cells through clathrin-, caveolin-, and gap-mediated endocytosis. Furthermore, the enhanced transport ability of CDPs was observed in a study on Caco-2/HT-29-MTX cocultured cells. CDPs exhibited a prolonged hypoglycemic response with a relative bioavailability of 9.2% in diabetic rats after oral administration. In conclusion, CDPs can target small intestinal goblet cells and have a beneficial effect on the oral administration of macromolecular peptides as a nanometer-sized carrier.
研究领域:
药剂学
·
生物医学工程
·
糖尿病治疗
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Shuang Zhao,Cong Shi,Hongyang Hu,Zhengping Li,Gang Xiao,Qiaochun Yang,Peng Sun,Linyang Cheng,Wencheng Niu,Jinshun Bi,Zhao Yue
In this paper, a portable real-time sensing device was built for Concanavalin A (Con A) and glucose detection using a smartphone. The ion-sensitive field-effect transistor (ISFET) functioning at a low working point was selected as a small-size, low-power transducer, and dextran-capped silver nanoparticles (Dex-AgNPs) served as sensitive nanoprobes on the ISFET gate. Using the affinity between Con A and carbohydrates, Con A can be captured, and thus directly detected by the ISFET/Dex-AgNPs unit; then glucose can be determined indirectly by removing Con A from the ISFET/Dex-AgNPs/Con A unit via competition with dextran . The mechanism of this competition does less harm to the sensor, allows the reusability of the sensing device, and overcomes the Debye screening of the FET device in saline solutions . Powered by a button cell, the handheld device attains excellent Con A (0.16 ng mL −1 ) and glucose (10 nM) detection limit, and can practically be used for at least 20 days.
研究领域:
生物传感器
·
纳米技术
·
即时检测
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2014
·
IF 1.357
Zhang Jianping,Guo Jianfeng,Zhang Yao,Yang Jiao
The graft polymer Dex-g-PSSS was obtained through poly(sodium 4-styrene sulfonate) (PSSS) grafted on dextran(Dex) by using the cerium salt–hydroxyl group redox initiation system. The cross-linked microspheres C(Dex-g-PSSS) were synthesized by suspension polymerization with epichlorohydrin as the cross-linking agent. The chemical structure and physicochemical characteristics of C(Dex-g-PSSS) microspheres were represented by infrared spectroscopy (FTIR), optical microscope, and zeta potential analysis. The aim of the study is to constitute a colon-specific drug delivery system via molecular design, using C(Dex-g-PSSS) microspheres as the drug-carrying material and taking 5-fluorouracil (5-FU) as the model drug. The drug-carrying ability and mechanism of the cross-linked microspheres C(Dex-g-PSSS) for 5-FU were investigated. Finally, in vitro release tests for the drug-carrying microspheres were conducted. The experimental results show that in the medium with pH 2, the cross-linked microspheres C(Dex-g-PSSS) exhibit a strong adsorption ability for 5-FU because of strong electrostatic interactions and have an adsorption capacity of 154 ± 7.5 mg/g, displaying high drug-carrying efficiency. The in vitro release behavior of the drug-carrying microspheres is highly dependent on pH and dextranase. In the medium with pH 2, the drug-carrying microspheres do not release the drug and in the medium with pH 1, they release a little, whereas in the medium with pH 7.4, a sudden delivery phenomenon of the drug will occur, and in the presence of dextranase, a more sudden delivery phenomenon of the drug will occur, displaying an excellent colon-specific drug delivery behavior.
研究领域:
药剂学
·
生物材料学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2023
·
IF 6.7
Yaqing Liu,Zongzhang Wang,Yiru Wang,Yushuo Feng,Mengjiao Xu,Xiaoqian Ma,Qianqian Shi,Huaping Deng,Fangfang Ren,Yong Chen,Hongmin Chen
Rheumatoid arthritis (RA) is a common autoimmune disease, and the inflammatory response during its development can lead to joint cartilage and bone damage up to disability. Dexamethasone (DEX) can effectively alleviate the inflammatory response in RA, but the severe adverse effects that occur after long-term administration limit its clinical development. Herein, we propose a Ca-DEX biomineralization-inducing nut (CaCO3-DEX) with controlled-release properties for mitigating the toxic side effects of DEX in RA treatment, especially the damage to cartilage and bone. CaCO3-DEX releases the drug and Ca2+ preferentially in an inflammatory environment. Both in vitro and in vivo studies demonstrate that CaCO3-DEX significantly reduces the secretion of pro-inflammatory factors and inhibits ROS production in vitro, as well as demonstrates superior pro-biomineralization and osteogenic differentiation potential. In the collagen-induced rheumatoid arthritis model (CIA model), CaCO3-DEX significantly reduces the clinical score of arthritis in mice, and imaging results show a noticeable relief of edema and bone erosion in CIA model mice treated with CaCO3-DEX, while inflammatory factors at the injury areas are significantly reduced, which provides favorable protection to cartilage and bone.
研究领域:
药理学
·
风湿病学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Research
·
2021
·
IF 0
Lijun Cai,Guopu Chen,Yuetong Wang,Cheng Zhao,Luoran Shang,Yuanjin Zhao
Microparticles with strong adherence are expected as efficient drug delivery vehicles. Herein, we presented an ingenious hydrogel microparticle recapitulating the adhesion mechanism of Boston ivy tendrils adhesive discs (AD) for durable drug delivery. The particles were achieved by replicating a silica colloidal crystal aggregates assembled in a droplet template after rapid solvent extraction. Due to their unique shape, the nanostructure, and the sticky hydrogel component, such novel microparticles exhibited prominent adhesive property to the wet tissue environment. It was demonstrated that the bioinspired microcarriers loading with dexamethasone had a good therapeutic effect for ulcerative colitis due to the strong adhesion ability for prolonging the maintenance of drug availability. These virtues make the biomimetic microparticles potentially ideal for many practical clinical applications, such as drug delivery, bioimaging, and biodiagnostics.
研究领域:
生物材料学
·
药剂学
·
生物医学工程
✓ 已验证
DOI
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2019
·
IF 3.073
Tingting Guan,Qin Yan,Lizhi Wan,Songhong Zhang,Linhong Xu,Jianli Wang,Junxian Yun
For preparing cryogel beads by the micro-flow-focusing and cryo-polymerization method, liquid–liquid flow patterns and slug characteristics for the system of Dex-MA and ethyl acetate and the system of Dex-MA-HEMA and ethyl acetate in a cross-shaped square microchannel were studied. The aqueous solutions of Dex-MA-HEMA and Dex-MA were used as the dispersed phase and ethyl acetate as the continuous phase. The formation mechanism of the slug flow was illustrated by analyzing the interaction of the interfacial tension between the dispersed and continuous phases, inertial forces and viscous forces of the dispersed phase and the continuous phase. The flow pattern maps for the two systems were proposed and five regimes were identified, i.e., the slug flow, bubbly flow, jetting flow, unstable flow and stratified flow. The slug lengths and the droplet diameters were also measured experimentally and correlated. The results showed that the formation of the slug flow was related to the interaction of the forces of the continuous phase and dispersed phase. The inertial and viscous force of the continuous phase promoted while the viscous force of the dispersed phase resisted the formation of the slug flow. The interfacial tension and the inertial force of the aqueous phase were shifting their effects which either promoted or resisted the formation of the slug flow. The flow pattern maps have a good agreement with those proposed in the literatures. The velocity of the continuous phase was more influential on the length of the slug segment. The obtained correlation of slug length and the droplet diameter could be helpful to the preparation of the cryogel beads.
研究领域:
生物工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS LETTERS
·
2016
·
IF 2.437
Peng Zou,Hairong Liu,Yongsheng Li,Jiying Huang,Yao Dai
The proliferation of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro is a key challenge for cartilage tissue engineering and their clinical applications. In this study, novel dextran grafting PHBV (PHBV-DEX) fibrous scaffolds were fabricated via surface modification. Compared with the electrospun PHBV fibrous scaffolds, dextran modified ones exhibited significantly enhanced surface hydrophilicity and bioactivity. The water contact angle (WCA) of those scaffolds was reduced from 117.3±4.30° to 56.03±1.13° after dextran modification. PHBV-DEX scaffold significantly promoted the growth of BMSCs compared with unmodified one, suggesting that it can be potentially applied in tissue engineering.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xingfan Li,Rong Ji,Linyan Duan,Zhizhong Hao,Yujing Su,Hao Wang,Fangxia Guan,Shanshan Ma
The adverse microenvironment, including neuroinflammation, hinders the recovery of spinal cord injury (SCI). Regulating microglial polarization to alleviate neuroinflammation at the injury site is an effective strategy for SCI recovery. MG53 protein exerts obvious repair ability on multiple tissues damage, but with short half-life. In this study, we composited an innovative MG53/GMs/HA-Dex neural scaffold using gelatin microspheres (GMs), hyaluronic acid (HA), and dextran (Dex) loaded with MG53 protein. This novel neural scaffold could respond to MMP-2/9 protein and stably release MG53 protein with good physicochemical properties and biocompatibility. In addition, it significantly improved the motor function of SCI mice, suppressed M1 polarization of microglia and neuroinflammation, and promoted neurogenesis and axon regeneration. Further mechanistic experiments demonstrated that MG53/GMs/HA-Dex hydrogel inhibited the JAK2/STAT3 signaling pathway. Thus, this MG53/GMs/HA-Dex neural scaffold promotes the functional recovery of SCI mice by alleviating neuroinflammation, which provides a new intervention strategy for the neural regeneration and functional repair of SCI.
研究领域:
神经科学
·
生物材料
·
再生医学
·
炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Rui Tan,Ziji OuBu,Qun Zhou,Juan Mu,Chenxi Yi,Xiaojun Luo
The presence of dexamethasone (DEX) residues and the illegal addition of DEX to food have garnered widespread public concern over recent decades. Based on the bicycled hairpin assembly (BHA) mechanism and the catalytic oxidase effect of urchin-shaped Au–Ag alloy loaded with Pt nanoparticles (Au@Ag@Pt), an accurate and ultrasensitive surface-enhanced Raman scattering (SERS)/colorimetric dual-mode method for DEX detection is pioneered. When DEX is introduced into the sensor system, the BHA cycle begins. The Fe3O4 nanosphere loaded with Au nanoparticles (Fe3O4@Au) surface facilitates the anchoring of a large amount of Au@Ag@Pt, resulting in synergistic signal amplification and facile magnetic separation from the complex system. Finally, the collected product effectively catalyzes the conversion of 3,3′,5,5′-tetramethylbenzidine (TMB) to the oxidized product, producing a strong SERS response, an ultraviolet–visible absorption signal, and color deepening. Our dual-mode platform achieves ultrasensitive detection of DEX, achieving a detection limit (LOD) of 6.61 × 10–17 M within the broad range of 10–16 to 10–9 M for SERS and a LOD of 8.81 × 10–13 and 6.98 × 10–13 M within the range of 10–12–10–5 M for the colorimetric method. Additionally, this platform shows high selectivity and satisfactory recovery rates for DEX in milk and pork samples, with recoveries of 92.3%–106.5% (RSDs <8.35%) for SERS and 95.2%–107.6% (RSDs <8.81%) for colorimetric analysis. This study demonstrates that by combining the BHA amplification process with multifunctional Au@Ag@Pt and Fe3O4@Au substrates, the DEX content in actual food samples can be accurately determined using a dual-mode approach that combines the high sensitivity of SERS with the simplicity of colorimetric analysis.
研究领域:
分析化学
·
食品安全
·
纳米生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）