SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Wenhao Guan,Zhijia Fang,Yinyan Chen,Yongbin Li,Zhilan Peng,Lijun Sun,Qi Deng,Ravi Gooneratne
As one of the most accumulative toxic heavy metals, cadmium (Cd) poses a major threat to human health. Bacterial siderophores, as small molecules with metal-absorbing ability, have great potential activity for Cd-reduction. In this study, the siderophore-producing bacterialstrain FCH-CR2 was isolated from a high-Cd contaminated soil using the CAS method. Leclercia adecarboxylata was identified through 16S rRNA sequence, homology analysis, colony morphology, physiological and biochemical tests. A siderophore, catechol type 2,3-dihydroxy-N-benzoyl- l -serine (DHBS) secreted by FCH-CR2, was purified using RP-HPLC and identified by LC-MS/MS. Intraperitoneal injection of DHBS significantly increased fecal Cd levels, and reduced Cd accumulation in organs. In density flooding theory (DFT) analysis, DHBS may bind to Cd via the hydroxyl site on the benzene ring. Besides, the isothermal titration calorimetry (ITC) assay revealed that the formation of Cd-DHBS is a spontaneous and endothermic reaction with ΔG = −21.4 kJ/mol and ΔH = 1.51 ± 0.142 kJ/mol.
研究领域:
环境科学
·
微生物学
·
化学
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DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Jing Yang,Xiaoye Feng,Haiqin Li,Pengtao Chang,Xiaojun Ren,Xiaochun Li
Chronic kidney disease (CKD) has become a public health burden, with a high mortality rate and a trend toward youthfulness. It is imperative to develop a home monitoring system for the early diagnosis and long-term monitoring of CKD serum biomarkers. However, due to the limitations of conventional centrifugation preprocessing of whole blood samples, the real-time detection platform cannot fully meet the requirement of obtaining quantitative results directly from whole blood. Herein, a novel smartphone-assisted microfluidic chip integration of a whole blood separation point-of-care testing (POCT) system was proposed to achieve accurate quantification of CKD biomarkers uric acid (UA), creatinine (CR), and albumin (Alb) based on 30 μL of whole blood (fingertip blood). The whole blood separation module allows rapid separation of plasma from whole blood samples by vertical flow. A smartphone App for automated image recognition and processing was developed, which was applied for at-home routine healthcare. The detection limits of UA, CR, and Alb were 0.1127 mmol/L, 0.2978 μmol/L, and 0.7696 mg/mL, respectively, meeting clinical requirements. Additionally, the quantitative performance of 83 clinical whole blood samples showed high consistency with the clinical analyzer (Beckman AU 5400 automatic biochemical analyzer). This system is simple to operate and user-friendly, providing an accurate, rapid, cost-effective, and reliable detection method for the early screening and real-time monitoring of CKD, which holds significant potential in enhancing home self-management of CKD.
研究领域:
生物医学工程
·
临床诊断
·
微流控技术
·
即时检测
·
慢性肾病
·
生物传感器
·
家庭医疗健康技术
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Cuicui Wang,Qing Xue,Haiqin Li,Hao Qi,Xiaochun Li
Hyperuricemia (HUA) has gradually become a public health burden as an independent risk factor for a variety of chronic diseases . Herein, a user-friendly point-of-care (POC) detection system (namely “Smart-HUA-Monitor”) based on smartphone-assisted paper-based microfluidic is proposed for colorimetric quantification of HUA urinary markers, including uric acid (UA), creatinine (CR) and pH. The detection limits of UA and CR were 0.0178 and 0.5983 mM, respectively, and the sensitivity of pH were 0.1. The method was successfully validated in artificial urine samples and 100 clinical samples. Bland-Altman plots showed a high consistency between μPAD and the testing instruments (HITACHI 7600 Automatic Analyzer, URIT-500B Urine Analyzer and AU5800B automatic biochemical analyzer) in hospital. Smart-HUA-Monitor provides an accurate quantitative, rapid, low-cost and reliable tool for the monitoring and early diagnosis of HUA urine indicators.
研究领域:
医学诊断
·
生物技术
·
微流控技术
·
即时检测
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lian Xia,Feng Luo,Xiaojuan Niu,Yue Tang,Yuangen Wu
Pb(II) is a prevalent heavy metal ion classified as a 2B carcinogen. Excessive intake of Pb(II) in the human body can damage the central nervous system, kidneys, liver, and immune system, leading to permanent brain damage, anemia, and cancer. Colorimetry can be applied to rapidly determine Pb(II) residues, but there are still many challenges in the accuracy and sensitivity of detection. Based on the inhibitory impact of Pb(II) on the oxidase-like activity of octahedral silver oxide (Ag 2 O), a colorimetric sensor with smartphone-assisted analysis for the Pb(II) detection was first developed. Herein, it has been found that Pb(II) can adsorb onto the surface of octahedral Ag 2 O, hindering the production of O 2 − in the reaction system. This ultimately results in the suppression of oxidase-like activity, leading to a lighter purple appearance of the colorimetric reaction solution. The sensor exhibits a high degree of sensitivity and a limit of detection (LOD) for Pb(II) was calculated as 2.2 μg L −1 . Hence, the developed colorimetric sensor with high sensitivity, excellent specificity, and high tolerance to sodium ions is hopeful to have practical applications in Pb(II) detection in environmental water samples. Moreover, the sensor will provide a novel strategy for heavy metal ion detection and other substances.
研究领域:
环境科学
·
分析化学
·
毒理学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-9
·
IF 5.479
He Shao-Bin,Chen Rui-Ting,Wu Yan-Yu,Wu Gang-Wei,Peng Hua-Ping,Liu Ai-Lin,Deng Hao-Hua,Xia Xing-Hua,Chen Wei
Platinum nanoparticles (Pt NPs) covered with a bovine serum albumin scaffold and a particle size of 1.5 nm (BSA-Pt S NPs) are shown to display enhanced multiple enzyme-mimicking activities including peroxidase, oxidase, and catalase-like activities. The peroxidase-like activity is characterized by robustness and low signal background. BSA-Pt S NPs were used to design colorimetric assays for H 2 O 2 and glucose. H 2 O 2 latter reacts with 3,3′,5,5′-tetramethylbenzidine in the presence of BSA-Pt S NPs to form a blue product with an absorption maximum at 652 nm. The assay works in the 5–250 μM H 2 O 2 concentration range. The glucose assay is based on its glucose oxidase-catalyzed oxidation to produce gluconic acid and H 2 O 2 which then is colorimetrically quantified. Response is linear in the 10–120 μM glucose concentration range, and the detection limit is 2 μM (at S/ N = 3). The method correlates well with the glucose standard method (R 2 = 0.997 in the 95% confidence interval) which confirms that glucose in human serum has been successfully detected.
Improved enzymatic assay for hydrogen peroxide and glucose by exploiting the enzyme-mimicking properties of BSA-coated platinum nanoparticles.
研究领域:
生物化学
·
医学检测
·
纳米材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-11
·
IF 6.232
Li Fang,Hu Yuting,Zhao Anqi,Xi Yachao,Li Zimu,He Jianbao
β -cyclodextrin-functionalized porous
Pd@Au nanostructures ( β -CD-Pd@Au) with intrinsic
and enhanced peroxidase-like activity were successfully synthesized by a two-step
method. The synthesized β -CD-Pd@Au can
efficiently catalyze the oxidation of various substrates, such as
3,3′,5,5′-tetramethylbenzidine (TMB), mixture of 4-amino antipyrine (4-AAP) and
3,5-dichloro-2-hydroxy acid sodium (DHBS) (4-AAP/DHBS), and mixture of 4-AAP and
N-Ethyl-N-(3-sulfopropyl)-3-methyl-aniline sodium salt (TOPS) (4-AAP/TOPS), by
H 2 O 2 to generate visual blue,
purple, and pink color, respectively. The UV-vis absorbance peak of the three β -CD-Pd@Au catalyzed the chromogenic reaction
system located at 650 nm, 510 nm, and 550 nm, respectively. The β -CD-Pd@Au-catalyzed
TMB-H 2 O 2 chromogenic reaction
exhibited higher absorbance intensity, catalytic efficiency, and color stability in
comparison to 4-AAP/DHBS-H 2 O 2 and
4-AAP/TOPS-H 2 O 2 chromogenic
reactions. The catalytic activity of β -CD-Pd@Au
was enhanced about 4-fold compared to that of Pd@Au in terms of
K cat for
H 2 O 2 . Using TMB as chromogenic
substrate, a colorimetric assay was fabricated for the determination of
H 2 O 2 with a detection limit of
2.78 μM (absorbance at 650 nm). The colorimetric determination of glucose with a
detection limit of 9.28 μM was further achieved by coupling with glucose oxidase
enzymatic reaction, indicating the versatility of the β -CD-Pd@Au-based detection strategy. A paper-based detection method
coupled with smartphone for fast visual and instrument-free detection of glucose was
further developed. Finally, the developed colorimetric assay and paper-based
detection method were successfully applied to the determination of glucose in human
serum sample.
Graphical abstract
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Fang Li,Xu Wang,Jiachang Liu,Yuting Hu,Jianbo He
In this work, a double-layered microfluidic paper-based device (μPAD) with multiple colorimetric indicators was presented for the sensitive simultaneous detection of four kinds of small biomolecules, including glucose, uric acid, lactate, and choline in mixture. The double-layered μPAD were composed of the top detection layer and the bottom auxiliary layer to construct 3D microfluidic channels. The detection zones on the top detection layer were modified with various colorimetric reagents, different kinds of oxidase (glucose oxidase, uricase, lactate oxidase or choline oxidase) and horseradish peroxidase (HRP). The immobilized chromogenic reagents could be oxidized by the generated H 2 O 2 from the specific enzymatic reactions between the oxidases and their corresponding substrates to form different colored products with co-immobilized HRP as catalyst. As a result, four linear calibration curves were obtained for the determination of glucose (0.01–10.0 mmol/L), uric acid (0.01–5.0 mmol/L), lactate (0.04–10.0 mmol/L), and choline (0.04–24.0 mmol/L), respectively. The proposed double-layered μPAD with multiple colorimetric indicators exhibited good colorimetric performance, effectively improved sensitivity, and extended linear range, which was further applied to the simultaneously determination of glucose, uric acid, lactate, and choline in serum samples.
研究领域:
分析化学
·
生物传感器
·
微流控技术
·
生物医学工程
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 10
·2647-2655
·
IF 3.307
Wang Xu,Li Fang,Cai Ziqi,Liu Kaifan,Li Jing,Zhang Boyang,He Jianbo
In this work, a multilayer-modified paper-based colorimetric sensing platform with improved color uniformity and intensity was developed for the sensitive and selective determination of uric acid and glucose with smartphone as signal readout. In detail, chitosan, different kinds of chromogenic reagents, and horseradish peroxidase (HRP) combined with a specific oxidase, e.g., uricase or glucose oxidase (GOD), were immoblized onto the paper substrate to form a multilayer-modified test paper. Hydrogen peroxide produced by the oxidases (uricase or GOD) reacts with the substrates (uric acid or glucose), and could oxidize the co-immoblized chromogenic reagents to form colored products with HRP as catalyst. A simple strategy by placing the test paper on top of a light-emitting diode lamp was adopted to efficiently prevent influence from the external light. The color images were recorded by the smartphone camera, and then the gray values of the color images were calculated for quantitative analysis. The developed method provided a wide linear response from 0.01 to 1.0 mM for uric acid detection and from 0.02 to 4.0 mM for glucose detection, with a limit of detection (LOD) as low as 0.003 and 0.014 mM, respectively, which was much lower than for previously reported paper-based colorimetric assays. The proposed assays were successfully applied to uric acid and glucose detection in real serum samples. Furthermore, the enhanced analytical performance of the proposed method allowed the non-invasive detection of glucose levels in tear samples, which holds great potential for point-of-care analysis.
ᅟ
研究领域:
分析化学
·
即时检测
·
生物传感器
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jia Xu,Habib Khan,Li Yang
While colorimetric-based assays are very convenient to determine biomarkers in point-of-care testing (POCT), they often suffer from pretreatment procedures for separation of plasma or serum from whole blood samples. Here, we report a simple colorimetric paper-based analytical device (c-PAD) that is capable of performing sample-to-answer analysis by directly dropping the whole blood sample on paper. This is accomplished by utilizing sodium alginate hydrogel, which exhibits a nanometer-scale porous structure to effectively prevent the passage of large red blood cells and hemoglobin molecules, to encapsulate enzymes and chromogenic reagents. As the small targets in the blood sample enter the sensing region to trigger a chromogenic reaction, the resulting color signal is recorded by a smartphone. The interference from the red blood to the color signal can be completely avoided without the requirement of any separation process. The analytical performance of the method is evaluated by assaying glucose in real blood samples. The results show that rapid and accurate analysis can be achieved with the limit of detection as low as 0.12 mM. In addition, simultaneous detection of different targets (glucose, cholesterol, and triglycerides) in whole blood can be achieved by fabricating c-PAD with multiple sensing regions. Owing to its several essential advantages including an extremely simple procedure for fabrication, sample-to-answer analysis without tedious pretreatment, and capability to perform high-throughput analysis, the proposed c-PAD will be of great value in POCT applications of whole blood samples.
研究领域:
生物医学工程
·
分析化学
·
即时诊断
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Jie Li,Youcai Lu,Shiyu Ma,Zhongjun Li,Qingchao Liu
Li–O 2 battery has attracted wide attention because it can satisfy the need for a new energy storage system with high energy density. However, poor oxygen reduction (ORR)/oxygen evolution (OER) kinetics involving Li 2 O 2 formation/decomposition is a major obstacle to its development. By designing suitable cathode with desired structure and surface electronic states, the formation/morphology of Li 2 O 2 and the reaction kinetics of charge process can be efficiently optimized simultaneously. In this work, a novel sulfur (S) and chlorine (Cl) co-doped porous carbon (CSC) were synthesized by one-step pyrolysis strategy, and the amount of heterogeneous atoms could be flexibly controlled by different calcination temperatures . Experimental combined with theoretical calculations show that the content of C Cl bond plays an important role in the adsorption of intermediate LiO 2 , thus regulating the morphology of Li 2 O 2 and ultimately affecting the release of battery energy. The C S bond mainly acts on the dynamics of the ORR and OER processes, which has an important influence on improving the round-trip efficiency of the battery. Benefiting from the synergistic effect of heteroatoms , the electrochemical performances of the Li–O 2 battery, including round-trip efficiency, discharge capacity and cycling stability are greatly improved.
研究领域:
材料科学
·
能源存储与转换
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Junjun Wang,Yue Tang,Jia Zheng,Zhengmin Xie,Jianli Zhou,Yuangen Wu
Herein, a colorimetric biosensor for histamine detection in meat is first established based on the enhancement of DNAzyme with peroxidase-mimic activity. Histamine can boost the generation of G-quadruplex sequences, and make them more easily bond with hemin to produce many DNAzyme molecules. In addition, histamine increases the affinity of DNAzyme to the substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the obtained DNAzyme can catalyze H 2 O 2 and dissolved oxygen to produce many reactive oxygen species (ROS), which cause the TMB molecule to lose two electrons and generate yellow products, exhibiting a clear absorption peak at 450 nm. The colorimetric biosensor has excellent sensitivity, and the detection limit is as low as 38 μg·L −1 for histamine. Moreover, the biosensor has high selectivity and anti-interference ability, and exhibits a good recovery rate in actual meats. The above results show that the strategy has potential for application in the detection of trace histamine in meats.
研究领域:
生物化学
·
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）