POLYHEDRON
·
2025
·
IF 2.6
Haoyun Lu,Kun Zhang,Liping Gao
The high-efficient detection of specific inorganic ions in H 2 O is extremely important, and monitoring S 2− and Ag + is of great significance because their abnormal levels will lead to many environmental and health problems. Herein we propose a long-wavelength absorbing carbocyanine dye DIDS-Li as a colorimetric probe to detect S 2− and Ag + based on color changes induced by structural changes of DIDS-Li. Sensing results suggest that the S 2− will cause the azure DIDS-Li to become colorless and the Ag + causes DIDS-Li to become bright yellow. This colorimetric probe also shows the good immunity to interference, a wide linear response range, and quantitative accuracy of S 2− and Ag + detections in natural water samples. Meanwhile, we also develop a smartphone analysis method that greatly improves the real-time detection ability of DIDS-Li for S 2− and Ag + . This work elucidates the relationship between carbocyanine dye and inorganic ions which offers a new means for efficient detection of S 2− and Ag + and the new idea for the application of carbocyanine dyes in colorimetric detection.
研究领域:
分析化学
·
环境监测
·
无机离子检测
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
NEUROSCIENCE LETTERS
·
2017
·
IF 2.18
Tingting Yang,Zhenzhou Lin,Ling Xie,Yao Wang,Suyue Pan
Vasogenic edema induced by blood brain barrier disruption and neuronal loss play an important role in the epileptogenic process. 4,4′- diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) is a commonly used anion channel inhibitor that has been reported to exert an anticonvulsant effect in rat hippocampus in vitro. The present study aimed to investigate whether DIDS could prevent epileptogenic process in rat lithium-pilocarpine model of temporal lobe epilepsy. The tight junction proteins and serum extravasation were examined in the piriform cortex 3 days after status epilepticus. The findings showed that status epilepticus induced vasogenic edema. Based on these findings, rats were intracerebroventricularly infused with saline and DIDS 1 week after surgery, DIDS reduced vasogenic edema and prevented neuronal loss following status epilepticus in the piriform cortex. Moreover, spontaneous recurrent seizures were recorded by continuous video monitoring. DIDS significantly reduced the frequency and duration of spontaneous recurrent seizures from day 28 to day 42 post status epilepticus. These findings demonstrated that DIDS attenuated vasogenic edema and neuronal apoptosis and might exert disease-modifying effect in animal model of temporal lobe epilepsy. These results explored a novel therapeutic strategy for treatment of epilepsy.
研究领域:
神经科学
·
药理学
·
癫痫研究
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DOI
PubMed
高影响因子
Communications Biology
·
2024
·Vol. 7
·期 1
·1-13
·
IF 5.2
Lai Cuixin,Yang Lina,Pathiranage Vishaka,Wang Ruizhao,Subach Fedor V.,Walker Alice R.,Piatkevich Kiryl D.
Genetically encoded fluorescent biosensors became indispensable tools for biological research, enabling real-time observation of physiological processes in live cells. Recent protein engineering efforts have resulted in the generation of a large variety of fluorescent biosensors for a wide range of biologically relevant processes, from small ions to enzymatic activity and signaling pathways. However, biosensors for imaging sulfate ions, the fourth most abundant physiological anion, in mammalian cells are still lacking. Here, we report the development and characterization of a green fluorescent biosensor for sulfate named Thyone. Thyone, derived through structure-guided design from bright green fluorescent protein mNeonGreen, exhibited a large negative fluorescence response upon subsecond association with sulfate anion with an affinity of 11 mM in mammalian cells. By integrating mutagenesis analyses with molecular dynamics simulations, we elucidated the molecular mechanism of sulfate binding and revealed key amino acid residues responsible for sulfate sensitivity. High anion selectivity and sensitivity of Thyone allowed for imaging of sulfate anion transients mediated by sulfate transporter heterologously expressed in cultured mammalian cells. We believe that Thyone will find a broad application for assaying the sulfate transport in mammalian cells via anion transporters and exchangers.
研究领域:
细胞生物学
·
分子生物学
·
生物物理学
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Jie Zhong,Hang Wei,Jian-Xiong Xie,Yu-Hui Wu,Bing Tang,Qi Zou,Peng-Ran Guo,Zhi-Liang Chen
The use of wetland plants in the context of phytoremediation is effective in the removal of antibiotics from contaminated water. However, the effectiveness and efficiency of many of these plants in the removal of antibiotics remain undetermined. In this study, the effectiveness of two plants— Phragmites austr alis and Iris pseudacorus —in the removal of tetracycline (TC) in hydroponic systems was investigated. The uptake of TC at the roots of I. pseudacorus and P. australis occurred at concentrations of 588.78 and 106.70 μg/g, respectively, after 7-day exposure. The higher uptake of TC in the root of I. pseudacorus may be attributed to its higher secretion of root exudates , which facilitate conditions conducive to the reproduction of microorganisms. These rhizosphere-linked microorganisms then drove the TC uptake, which was higher than that in the roots of P. australis . By elucidating the mechanisms underlying these uptake-linked outcomes, we found that the uptake of TC for both plants was significantly suppressed by metabolic and aquaporin inhibition, suggesting uptake and transport of TC were active (energy-dependent) and passive (aquaporin-dominated) processes, respectively. The subcellular distribution patterns of I. pseudacorus and P. australis in the roots were different, as expressed by differences in organelles , cell wall concentration levels, and transport-related dynamics. Additionally, the microbe-driven enhancement of the remediation capacities of the plants was studied comprehensively via a combined microbial-phytoremediation hydroponic system. We confirmed that the microbial agents increased the secretion of root exudates, promoting the variation of TC chemical speciation and thus enhancing the active transport of TC. These results contribute toward the improved application of wetland plants in the context of antibiotic phytoremediation .
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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