ACS Sustainable Chemistry & Engineering
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2024
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IF 7.1
Lei He,Yongze Liu,Haomiao Zhu,Feng Chen,Jiong Shi,Panpan Song,Fuqi Feng,Juqing Cui,Xiaofeng Ma,Jun Zhang
Nasal diseases are pervasive infections that can impair the quality of life of sufferers. In this study, a flexible modified triplochitin scleroxylon (MTS)-based Janus hydrogel (MTS@P/DLT) was obtained by disrupting the homogeneity of the system using DL-α-tocopherol (DLT) with flexible wood as the framework and poly(vinyl alcohol) (PVA) as the outer matrix. At a PVA concentration of 10 wt %, DLT formed a homogeneous layer, as revealed by electron microscopy. Mechanical property analysis showed that chemical and hydrogel modifications significantly increased the flexibility and toughness of the hydrogel. Microscopic and macroscopic assessment of the characteristics of the two sides of the MTS@P/DLT hydrogel revealed that one side of the DLT film had antiadhesion effects. Molecular dynamics simulation was used to determine the movement law of DLT in a PVA solution. The stronger intermolecular force between DLT and PVA than that between DLT and water, as well as between DLT molecules, enabled the formation of a homogeneous DLT film by controlling the concentration of PVA. In vitro and in vivo models demonstrated the potential of MTS@P/DLT to resist infection and facilitate recovery. Thus, MTS@P/DLT is promising for medical use.
研究领域:
材料科学
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医学
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生物工程
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Wenjing Li,Ling Xiao,Juan Qi,Yuanyuan Zhang,Zhu Huang,Liangliang Zhu
Inhibition of gut β-glucuronidase (GUS) protects the gastrointestinal tract by reducing local exposure to toxic aglycones from inactive glucuronides. Development of highly safe GUS inhibitors has attracted extensive attentions. The current study demonstrates that natural and synthetic vitamin E (VE) analogues all display potent inhibition toward GUS. dl-α-tocopherol acts as the most potent inhibitor with the Ki value of 0.14 μM, while the other analogues have Ki values below 15.1 μM. Docking analysis indicates that the amino residues in the active pocket of GUS form various hydrophobic interactions with both the chromanol ring and side chain of VE, as well as hydrogen bonds with the hydroxyl group. It is predicted that GUS inhibition readily occurs owing to the daily intake of common plant foods. The current study offers highly safe inhibitors for GUS, as well demonstrates a novel nonantioxidant target for VE providing new insights into the vitamin’s gastrointestinal protection.
研究领域:
生物化学
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药理学
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分子生物学
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酶学
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营养学
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PubMed
高影响因子
Frontiers in Plant Science
·
2025
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IF 4.8
Kai Zhao,Yafei You,Daiqian Deng,Qiujun Du,Zixian Guo,Lei Liu,Xiangcai Meng
Introduction Both Paeoniae Radix Alba (RAP) and Paeoniae Radix Rubra (RRP) are important botanical drugs used in Asian countries. Although they are both derived from the roots of Paeonia lactiflora Pall., they exhibit distinct pharmacological properties due to differences in germplasm and processing methods. Due to overwhelming market demand, the cultivated varieties have become the primary source to compensate for insufficient wild resources, which have led to decreased medicinal quality. This study aimed to address this quality decline and put forward a hypothesis that exogenous nitric oxide (NO) induces the reactive oxygen species (ROS)-mediated enhancement of secondary metabolism in fresh roots of P. lactiflora , thereby improving medicinal quality. Methods Fresh roots of P. lactiflora germplasm for Paeoniae Radix Rubra production (RRP-germplasm) and for Paeoniae Radix Alba production (RAP-germplasm) were treated with sodium nitroprusside (SNP) at concentrations of 0.0, 0.1, 0.5, or 2.5 mmol/L to induce ROS bursts. Results In the fresh roots of RRP-germplasm treated with 0.5 mmol/L SNP, the secondary metabolites paeoniflorin, albiflorin, oxypaeoniflorin, gallic acid, catechin, and paeonol were elevated by 19.1%, 205.4%, 115.4%, 19.9%, 201.0%, and 585.2%, respectively, and in the fresh roots of RAP-germplasm treated with 2.5 mmol/L SNP, the major secondary metabolites paeoniflorin, albiflorin, oxypaeoniflorin, gallic acid, catechin, and benzoic acid showed increases of 25.4%, 70.4%, 95.1%, 6.7%, 86.5%, and 33.6%, respectively. Moreover, experiments involving combined treatment with SNP and ROS scavengers demonstrated that ROS act as the key mediator linking exogenous NO to the secondary metabolism of P. lactiflora : scavenging ROS significantly attenuated the SNP-induced accumulation of target secondary metabolites. Discussion Combined with the above findings of SNP promoting secondary metabolite synthesis, this study confirms that exogenous NO can improve the quality of cultivated RAP and RRP via ROS-mediated secondary metabolism, and clarifies the NO-ROS-secondary metabolism regulatory axis, offering insights for other medicinal plants’ quality improvement.
研究领域:
植物生理学
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植物化学
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药用植物生物学
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活性氧信号转导
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次生代谢调控
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PubMed
高影响因子
FOOD CHEMISTRY
·
2026
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IF 9.8
Wen-Sen He,Na Wei,Jingyu Li,Qingzhi Wang,Haonan Yang,Chen Tan,Shouhe Huang,Huafang Ding,Jianhua Zhu,Hanyu Zhu,Zhen-Yu Chen
Interplay between hydrophobic and hydrophilic antioxidants in bilayer emulsions remains unclear, especially within the unique, thicker interface of layer-by-layer assembled systems. This study investigates the antioxidant behavior and interaction mechanism of α-tocopherol (hydrophobic) and l -ascorbic acid (hydrophilic) in tree peony seed oil (TPSO) bilayer emulsions stabilized by whey protein isolate and sodium alginate. At equivalent concentrations, α-tocopherol exhibited stronger antioxidant activity than l -ascorbic acid. When combined, α-tocopherol and l -ascorbic acid yielded a synergistic effect ( Q value = 1.3–1.6). In combined treatments, the proportion of l -ascorbic acid distributed in the aqueous phase decreased compared to its individual use, while its presence at interfacial layer increased correspondingly. This distribution significantly enhanced the oxidative stability of the bilayer emulsion and effectively slowed TPSO degradation. Mechanistically, the close proximity of l -ascorbic acid and α-tocopherol at interface facilitated electron transfer, allowing l -ascorbic acid to regenerate α-tocopherol from its radical form and synergistically enhance oxidative stability.
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高影响因子
Discover Nano
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2026
·Vol. 21
·期 1
·334
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IF 6.7
Xuan Shaoyan,Yang Shenglei,Mao Yuqin,Chen Zhiyong,Zhu Yue,Zhou Ying,Huang Jie,Zhu Xiuliang,Wang Zuhua
Lung cancer remains one of the most prevalent and lethal malignancies worldwide. Oridonin (ORI) exhibits remarkable anti-lung cancer activity. Nevertheless, its clinical application in lung cancer therapy is severely hindered by poor solubility, fast metabolism, insufficient tumor penetration, and low selectivity. In this study, we developed a redox-responsive polymeric micelle (HA-cys-TOS) targeting the CD44 receptor to improve ORI bioavailability and tumor targeting. The polymer was synthesized via esterification and verified by 1 H NMR. Our data showed that ORI@HA-cys-TOS exhibited good stability, biosafety and redox-responsive sustained release. Compared to HepG2 cells, ORI@HA-cys-TOS exhibited significantly enhanced internalization ability in A549 cells, likely due to HA binding to the CD44 receptor overexpressed on A549 cells. Unexpectedly, the enhanced targeted accumulation of ORI@HA-cys-TOS micelles in vivo was also observed in both subcutaneous and in situ lung cancer tissues. In vivo and in vitro antitumor studies demonstrated that ORI@HA-cys-TOS micelles exhibited significantly greater antitumor activity compared to free ORI. Notably, no side-effects were observed during the 21 days experimental period. This study provides an experimental basis for advancing ORI toward clinical personalized lung cancer therapy by targeting CD44 and responding to the tumor redox microenvironment.
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FOOD CHEMISTRY
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2013
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IF 3.334
Xican Li
The deoxyribose degradation assay is widely used to evaluate the hydroxyl ( OH) radical-scavenging ability of food or medicines. We compared the hydroxyl radical-scavenging activity of 25 antioxidant samples prepared in ethanol solution with samples prepared after removing the ethanol (residue). The data suggested that there was an approximately 9-fold difference between assay results for the ethanol solution and residue samples. This indicated a strong alcoholic interference. To further study the mechanism, the scavenging activities of 18 organic solvents (including ethanol) were measured by the deoxyribose assay. Most pure organic solvents (especially alcohols) could effectively scavenge hydroxyl radicals. As hydroxyl radicals have extremely high reactivities, they will quickly react with surrounding solvent molecules. This shows that any organic solvent should be completely evaporated before measurement. The proposed method is regarded as a reliable hydroxyl radical-scavenging assay, suitable for all types of antioxidants.
研究领域:
生物化学
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食品科学
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药学
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PubMed
高影响因子
Aquaculture Reports
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2025
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IF 3.7
Guoyong Huang,Weibin Huang,Guangcai Fu,Yansheng Liu,Hengyang Song,Wenshan Cai,Yubin Liu,Yuxuan Han,Yilin Yao,Beiping Tan,Xiaohui Dong
This study assessed the efficacy of dietary vitamin E in ameliorating growth and liver function in Jinhu grouper compromised by the inclusion of oxidized fish oil (OFO; peroxide value: 121.68 mmol/kg) in the diet, compared with a fresh fish oil control (FFO; peroxide value: 1.25 mmol/kg). The eight-week feeding trial utilized five experimental diets that were isonitrogenous and isolipidic: FFO, OFO (0 mg/kg VE), or OFO supplemented with 200 (VE1), 400 (VE2), or 600 (VE3) mg/kg vitamin E. Fish receiving oxidized fish oil showed slower growth, indicated by reduced weight gain rate (WGR) and increased feed conversion ratio (FCR). Intake of oxidized lipids altered lipid metabolism, lowering serum total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) but elevating low-density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA) levels. Hepatic antioxidant capacity was compromised, as revealed by the concurrent suppression in both the activities and gene expression levels of key antioxidant enzymes (SOD, CAT, and GPx). Concurrently, oxidized fish oil induced liver inflammation, significantly upregulating pro-inflammatory cytokine genes (notably il-1β , il-6 , il-8 , tnf-α ), and caused histopathological damage including hepatocyte nuclear displacement and lipid droplet accumulation. Vitamin E supplementation ameliorated these effects. The VE2 group (400 mg/kg) maximally restored growth performanc, reduced serum MDA and LDL-C levels, enhanced hepatic antioxidant enzyme activities and gene expression, suppressed inflammatory cytokine expression, and attenuated histopathological lesions. In conclusion, dietary vitamin E supplementation at 400 mg/kg effectively counteracts growth impairment and hepatotoxicity induced by oxidized fish oil in Jinhu grouper.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Mengting Cui,Qingpeng Gao,Jinling Bai,Mengting Fu,Zhiyu Liu,Zhixin Ouyang,Liyun Kong,Le Ma
Conventional extraction techniques for fat-soluble nutrient analysis in foods may present certain limitations, including variable or limited efficiency, high solvent consumption, lengthy procedures, and potential environmental and health concerns. This study evaluates deep eutectic solvents (DESs) as a green and efficient alternative for extracting vitamin E and carotenoids from vegetables and fruits. Conductor-like screening model for real solvents (COSMO-RS) simulation and experimental screening identified the menthol-thymol (1:2) DES as optimal due to the balanced distribution of polar and nonpolar sites within its components. A novel dispersive liquid-liquid microextraction method based on this DES (DES-DLLME) was developed and integrated with HPLC for the simultaneous quantitation of vitamin E and carotenoids. The optimized method demonstrated excellent analytical performance with low detection limits (0.01-0.18 µg/mL), satisfactory recovery rates (80.04-111.95%), and high reproducibility (relative standard deviation, RSD < 16.83%). When applied to a range of Chinese food samples, the obtained nutrient profiles were largely consistent with USDA reference data, with observed variations likely due to factors such as geographic origin, cultivation practices, and post-harvest processing. This DES-based approach offers an efficient, reproducible, and environmentally friendly method for determining fat-soluble vitamins and carotenoids in complex food matrices, offering significant value for food quality assessment and nutritional research.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Shuai Xu,Dan Huang,Chongyang Liu,Yuan Gao,Qi Li,Xiuzhu Yu
In order to solve the problems of nutrient loss and flavor defect of the first-grade refined rapeseed oil, this study innovatively proposed an optimal strategy to enhance its nutrition and aroma. The microwaved-, roasted- and infrared-treated rapeseed cakes were mixed with primary rapeseed oil and then ultrasonically heated to obtain MCO, RCO and ICO, which were evaluated in terms of oxidation indexes, lipid concomitants and volatile aroma components. The results found that MCO showed the best comprehensive performances when the heating temperature was 120 °C: squalene, tocopherol and total phenol contents increased by 12–18 %, 20–30 % and 10–15 %, respectively, and key aroma substances (furfural) enhanced nutty notes. Correlation analysis showed that squalene ( r = 0.82) and polyphenols ( r = 0.79) had significant negative correlation with key flavor substances by inhibiting oxidation. This study established a process for synergistically enhancing rapeseed oil nutrition and flavor, providing a theoretical basis and technical path for high-value-added product development.
研究领域:
食品科学
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营养学
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风味化学
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油脂加工技术
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食品工程
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EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2025
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IF 1.8
Yufeng Zhao,Dan Huang,Xiangyu Wang,Baijun Chu,Yuxiu Wen,Qi Li,Xiuzhu Yu
The variations in tocopherols and phytosterols during the oxidation of walnut oils (Wen-185, Xiangling, and Dama oils) were analyzed, and their antioxidant effects were compared. Under both ambient temperature and accelerated oxidation conditions, the degradation order of tocopherols in walnut oils was α-tocopherol > γ-tocopherol > δ-tocopherol. Phytosterols exhibited less degradation than tocopherols under both oxidation conditions, and tocopherols were more strongly associated with oil oxidation than phytosterols. When δ-tocopherol degradation reached 15%–20%, the peroxide value approached 20 meq/kg, highlighting its potential as an indicator of oil oxidation. Further studies on the antioxidant effectiveness of tocopherols revealed that the optimal effect was achieved as 400 mg/kg α-tocopherol was added at Day 16 under ambient temperature. These findings suggested that δ-tocopherol can serve as an indicator of walnut oil oxidation, and appropriately timed and dosed α-tocopherol supplementation can significantly delay oxidation in walnut oils. Practical Applications : This study compared the changes and degradation of tocopherols and phytosterols in different walnut oils. The results suggested that tocopherol degraded faster than phytosterol, with a closer correlation to oil oxidation, and α-tocopherol underwent degradation preferentially. Furthermore, the antioxidant effectiveness of tocopherols was analyzed. The results showed that proper quantity and timing of α-tocopherol addition can enhance antioxidant effect, and δ-tocopherol can assess the oxidation of walnut oils as an concomitant indicator. This study has expanded the application of endogenous concomitants in the antioxidant properties of walnut oils. It has laid a theoretical foundation for the antioxidant properties of walnut oils.
研究领域:
食品化学
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油脂科学
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抗氧化研究
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食用油
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氧化稳定性
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaowei Peng,Chenhao Jiang,Xunyao Zhang,Huijing Chen,Jianquan kan
Huajiao seed oil exhibits remarkable nutritional value owing to its abundant content of unsaturated fatty acids. However, its susceptibility to oxidative degradation continues to be a critical concern. Therefore, this study investigated the basic quality of Huajiao seed oil at varying oxidation degrees and dynamically tracked changes in its volatile compounds and lipid profiles using flavoromic and lipidomic approaches. During oxidation, seed oil color gradually shifted from orange-yellow to light yellow. Additionally, the α-tocopherol, squalene, and carotenoid levels progressively declined. After 15 days of oxidation, the linoleic and α-linolenic acid content markedly decreased, whereas the oleic acid, ( E )-2-hexenal, and ( E,E )-2,4-decadienal contents substantially increased. Flavoromic analysis revealed that aldehydes were the primary volatile compounds generated during oxidation. Lipidomic data demonstrated that saccharolipid levels considerably increased during oxidation, whereas glycerolipid levels markedly decreased. Overall, this study enhances the understanding of the oxidation process in Huajiao seed oil, which is of great significance for effectively inhibiting or delaying its oxidation.
研究领域:
食品科学
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分析化学
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脂质组学
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风味化学
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氧化稳定性
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植物生物化学
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高影响因子
AAPS PHARMSCITECH
·
2019
·Vol. 20
·期 2
·1-15
·
IF 2.608
Zhang Shudong,Wan Qiang,Xing Yidan,Ding Jiafeng,Yang Shizhuang,Sun Weiwei,Lu Mengmeng,Pan Baoliang
Drugs with pH-dependent solubility that have poor water solubility can be identified in the drug discovery pipeline. Some of them have poor oral absorption, which can result in insufficient efficacy. Micro-environmental pH-modifying solid dispersion (micro pHm SD) is a promising approach to overcome the poor oral absorption of these drugs. In the present study, toltrazuril (TOL), a weakly acidic drug with poor aqueous and pH-dependent solubility, was used as a model drug. Using micro pHm SD, a novel oral oil-based suspension of TOL SD (TSDS) was developed, and the stability of this formulation was evaluated based on particle size, settling volume ratio, redispersibility, thermal stability, and drug content. The optimized soybean oil-based TSDS (S-TSDS) had high physicochemical stability and good histocompatibility with common inflammatory reactions. The results of the in vitro dissolution analysis showed that S-TSDS rapidly and markedly released the drug and provided higher efficacy and longer persistence against coccidiosis (above 90.9%) in rabbits. This technique could increase the oral absorption and bioavailability of new drug candidates.
研究领域:
药剂学
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药物递送系统
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药学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）