CATALYSIS COMMUNICATIONS
·
2014
·
IF 3.32
Jian-yong Zheng,Yu-qiang Wang,Wei-feng Luo,Sha-sha Zhou,Qing Zhu,Xiang-xian Ying,Zhao Wang
A new esterase-producing strain ( Bacillus amyloliquefaciens WZZ002) that exhibits high hydrolytic activity, excellent enantioselectivity, and high substrate tolerance on Boc- dl -Alanine methyl ester was isolated from soil samples. The reaction temperature, pH, and neutralizer optima of the cell-mediated biocatalysis were 35 °C, pH 8.0, and NH 3 ·H 2 O, respectively. The optimal substrate concentration was 2 M, with a biocatalyst loading of 50 g/L. Results showed that the enantiomeric excess values of substrate and product were both greater than 99%. Thus, bioprocessing with the use of the isolated strain is a promising route for the commercial production of Boc- d -Ala-OMe.
研究领域:
生物化学
·
微生物学
·
制药工程
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yingying Guo,Yuxuan Mo,Miao Wang,Huihui Cui,Yanfeng Tang,Tongming Sun
Employing DL-Alanine (DL-Ala) as the structure-directing agent, hierarchical microspheres of ZnOHF have been designed with optimized geometric features in a facile one-step hydrothermal approach. Morphological characterizations demonstrated that the obtained hierarchical microspheres were formed by numerous nanotubes with outer diameter of 10 nm and wall thickness about 3.5 nm. A series of controllable experiments proved that DL-Ala and reaction time played vital roles in the formation of nanotubes-assembled hierarchical microspheres. By taking advantage of the optimized geometric features and large specific surface areas (271.06 m 2 /g), the obtained ZnOHF nanotubes-assembled microspheres were evaluated as adsorbents for the removal of various dyes in aqueous solution. Adsorption behavior conducted by batch mode experiments indicated that the adsorption was well described by Langmuir isotherm and pseudo-second-order model. The obtained hierarchical ZnOHF microspheres exhibited effective adsorption and selectively properties towards cationic dyes. For example, the maximum adsorption capacity of ZnOHF microspheres was 140.4 mg/g for malachite green (MG) at 298 K. Analysis of thermodynamic data suggested that it was an endothermic and spontaneous physical adsorption process. The fabrication strategy may provide new insights for designing other high-performance materials, and the novel hierarchical ZnOHF microspheres are promising adsorbents for wastewater purification.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
CHIRALITY
·
2018
·Vol. 30
·期 7
·907-912
·
IF 1.833
Jianyong Zheng,Xing Lan,Lijuan Huang,Yinjun Zhang,Zhao Wang
D-alanine is widely used in medicine, food, additives, cosmetics, and other consumer items. Esterase derived from Bacillus cereus WZZ001 exhibits high hydrolytic activity and stereoselectivity. In this study, we expressed the esterase gene in Escherichia coli BL21 (DE3). We analyzed the biocatalytic resolution of N-acetyl-DL-alanine methyl ester by immobilized whole E . coli BL21 (DE3) cells, which were prepared through embedding and cross-linking. We analyzed biocatalytic resolution under the optimal conditions of pH of 7.0, temperature of 40°C and substrate concentration of at 700 mM with an enantiomeric excess of 99.99% and e.e. p of 99.50%. The immobilized recombinant B . cereus esterase E . coli BL21 (DE3) cells exhibited excellent reusability and retained 86.04% of their initial activity after 15 cycles of repeated reactions. The immobilized cells are efficient and stable biocatalysts for the preparation of N-acetyl-D-alanine methyl esters.
研究领域:
生物化学
·
酶工程
·
制药工业
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产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2025
·
IF 4.2
Zihao Qiu,Gerile Naren,Rui Meng,Rui Liang
Rare-earth metal complexes have received wide attention for their excellent photo-physical properties and their light absorption capacity and energy transfer efficiency of organic ligands, which are the main factors affecting the photoluminescence of rare-earth metal co-mplexes. Therefore, in this study, four N -acyl amino acid praseodymium complexes, (Pr[CH 3 (CH 2 ) n CONHCH(CH 3 )COO] 3 , n = 4,6,8,10) with different carbon chain lengths were synthesized using H(hex-ala), H(oct-ala), H(dec-ala) and H(dod-ala) ligands and the Pr 3+ reaction method with the goal to obtain rare earth metal-organic complexes with good optical properties . The molecular structure of the praseodymium complexes was determined using CHN elemental analysis , 1 H NMR , and FT-IR spectroscopic analysis . Solubility tests in pure solvents have determined that Pr(oct-ala) 3 and Pr(dec-ala) 3 are highly soluble in polar solvents . However, Pr(dod-ala) 3 , which contains a long carbon chain and crystallizes easily, has a low solubility. The photophysical properties of the praseodymium complexes can be obtained by fluorescence spectroscopy in the visible region, which shows that the luminescence of the four praseodymium complexes occurs in the blue region, and the fluorescence lifetimes are about 0.32 μs. The effect of temperature change on the luminescence properties of the praseodymium complexes was analyzed by variable-temperature fluorescence spectroscopy . The fluorescence inte-nsity of Pr(dec-ala) 3 , which is more absorbent, is higher in the range of 25 °C–70 °C. The luminescence properties of praseodymium complexes in the near-infrared region were obtained by near-infrared emission spectroscopy , and three major emission intensities were found at 926 nm ( 1 D 2 → 3 F 2 ), 1001 nm ( 1 D 2 → 3 F 4 ), and 1376 nm ( 1 D 2 → 1 G 4 ), among which the intensity of the emission peaks of Pr(dod-ala) 3 was the strongest at 1001 nm, indicating a good match between the ligand-centered triplet state of Pr 3+ and the 1 D 2 emission state. In addition, in this study, the molecular structure of praseodymium complexes was geometrically optimized by density functional theory (DFT) method using Gaussian software, and the bond strengths and topological properties of the praseodymium complexes were obtained by Atoms in Molecules (AIM) theory analysis. The results show that there are closed-shell interactions between the ligand and Pr 3+ , in which Pr(oct-ala) 3 has the largest Pr–O bond strength among the complexes with different carbon chain lengths.
研究领域:
无机化学
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材料科学
·
光物理学
·
配位化学
·
理论化学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Zihao Qiu,Gerile Naren,Rui Meng,Rui Liang,Ziyang Chen
Four Pr-phen-acyl amino acid ternary complexes (Pr [CH 3 (CH 2 ) n CONHCH(CH 3 )COO] 3 ·phenanthroline, n = 4,6,8,10) were synthesized by a method used for optical materials that is based on solution chemical reactions. The molecular structure of the praseodymium complexes was determined using CHN elemental analysis, 13 C-Nuclear Magnetic Resonance ( 13 C NMR) spectroscopy, and Fourier Transform-Infrared (FT-IR) spectroscopy. X-ray diffraction and polarized light microscopy showed that Pr(hex‑ala) 3 ·phen, Pr(oct‑ala) 3 ·phen, and Pr(dec‑ala) 3 ·phen have good crystallinity and exhibit excellent optical anisotropy in the molten state. Ultraviolet-Visible (UV–Vis) spectroscopy revealed that the light absorption of the praseodymium complexes is in the range of 200 nm - 500 nm after the introduction of phenanthroline (phen). Fluorescence spectral analyses in the visible region, at an excitation wavelength of 417 nm, indicated that Pr(hex‑ala) 3 ·phen emits green light. In contrast, the other three praseodymium complexes with long carbon chains emit red light. Furthermore, phen significantly enhances the fluorescence intensity of the complexes and remarkably prolongs their fluorescence lifetimes. Variable-temperature fluorescence spectroscopy revealed that the praseodymium complexes maintained a high luminescence intensity throughout the heating process, ranging from 25 °C to 200 °C, which is attributed to their conjugated functionalities.
研究领域:
材料化学
·
发光材料
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配位化学
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光学材料
·
稀土化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2026
·
IF 9.5
Qiang Bi,Shiping Zhao,Yumiao Zhang,Cai Zhang,Kechun Wang,Jingfeng Tian,Yi Zhao,Juanqin Xue
The escalating threat of per and polyfluoroalkyl (PFAS) substances to human health and the ecological environment has intensified the need for advanced porous adsorbents. Herein, we report a series of mono and bifunctionalized MOF 808 materials grafted with fluoroalkyl and amino groups, systematically evaluating the contribution of the trifluoromethylalanine (TFMA) moiety toward broad spectrum PFAS uptake. Batch adsorption experiments reveal exceptional capacities of MOF 808 TFMA for perfluorooctanoic acid (PFOA) and perfluorobutanoic acid (PFBA), reaching 1558.3 and 605.4 mg g-1, respectively, with equilibration achieved within 30 minutes. Over 85% removal efficiency was maintained even at trace concentrations, alongside strong anti interference capability. The material retained 81% of its initial efficiency after five regeneration cycles using methanol–ammonia. Combined experimental characterization and density functional theory (DFT) calculations elucidate a multi synergistic site mechanism: amino enabled hydrogen bonding and electrostatic interactions dominate short chain PFAS capture, while fluorophilic and hydrophobic interactions enhance long chain PFAS adsorption. Notably, we identify for the first time an “adsorption trough” for perfluorohexanoic acid (PFHxA), attributed to a pore size–molecular dimension critical matching effect (ΔS° < 0), providing a theoretical early warning for avoiding synergistic failure in adsorbent design. The bifunctional MOF 808 TFMA demonstrates outstanding PFAS removal performance in real wastewater, offering a foundational strategy for the rational design of next generation PFAS adsorbents.
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Jiakang Han,Zihao Qiu,Gerile Naren,Rui Meng,Zikang Hu
In recent years, rare earth complexes containing amino acids have attracted much attention in numerous fields, such as light, electricity, catalysis, biological activity, drugs, etc. The rare earth element terbium is considered an important fluorescent metal due to its special electronic structure. It has excellent photophysical properties, such as high color purity, large Stokes shift, long excited state lifetime, and profuse energy levels. It has been used in the fields of fluorescent probes, medical radiation, and chemical sensors. Considering their wide applications, a series of acyl amino acid terbium complexes were synthesized using N-acyl amino acids as ligands, and their structures and photophysical properties were studied. The analysis of the theoretical and experimental results demonstrates that the energy transfer efficiency from ligands to the terbium ion emission levels increases with increasing alkyl carbon chain length, leading to differences in the luminescent intensity of the complexes. The energy transfer efficiency from ligands to rare earth ions was the result of a combination of the relative positions between the resonance energy levels of the central rare earth ion and the lowest triplet state energies of ligands as well as the intersystem crossing probability of the ligands. Moreover, the density functional theory (DFT) was used to determine the geometric structures and obtain 1 H NMR and FT-IR spectra of the terbium complexes. Their structures were further examined by comparing the calculated with the experimental spectra. The results show that the ligands coordinate to the central Tb 3+ through the bidentate chelation mode and that the theoretically determined structure was reliable.
研究领域:
化学
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材料科学
·
光物理学
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产品关联
DOI
高影响因子
Chemosensors
·
2025
·Vol. 13
·期 8
·291
·
IF 3.7
Xianghao Meng,Ronghua Zhang,Xinfeng Dong,Zhongxing Wang,Li Yu
Biothiols, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), are crucial for physiological regulation and their imbalance poses severe health risks. Herein, we developed a pH-responsive liquid crystal (LC)-based sensing platform for detection of biothiols by doping 4-n-pentylbiphenyl-4-carboxylic acid (PBA) into 4-n-pentyl-4-cyanobiphenyl (5CB). Urease catalyzed urea hydrolysis to produce OH−, triggering the deprotonation of PBA, thereby inducing a vertical alignment of LC molecules at the interface corresponding to dark optical appearances. Heavy metal ions (e.g., Hg2+) could inhibit urease activity, under which condition LC presents bright optical images and LC molecules maintain a state of tilted arrangement. However, biothiols competitively bind to Hg2+, the activity of urease is maintained which enables the occurrence of urea hydrolysis. This case triggers LC molecules to align in a vertical orientation, resulting in bright optical images. This pH-driven reorientation of LCs provides a visual readout (bright-to-dark transition) correlated with biothiol concentration. The detection limits of Cys/Hcy and GSH for the PBA-doped LC platform are 0.1 μM and 0.5 μM, respectively. Overall, this study provides a simple, label-free and low-cost strategy that has a broad application prospect for the detection of biothiols.
研究领域:
分析化学
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生物传感
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材料科学
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环境监测
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生物医学工程
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产品关联
DOI
高影响因子
Science Advances
·
2025
·Vol. 11
·期 32
·
IF 12.5
Chao Liang,Jingyang Zhao,Pingdong Wei,Kang Wei,Hanqing Jiang
Comprehensive liver function monitoring is vital for liver damage diagnoses. However, multiplexed testing with clinical accuracy often requires paralleled liquid manipulation to avoid reagent interference and nonspecific adsorption, making it restricted to centralized facilities and specialists. Here, we report an equipment-free, quantitative, and multiplexed point-of-care testing (POCT), the energy-embodied POCT (EE-POCT). The driving energy (pressured gas) and liquid reagents are embodied in separate rigid-flexible chambers through a mechanical pressure sensing mechanism to achieve a paralleled, interference-free, and multiple-reagent operation with no need for external equipment. The EE-POCT achieves quantitative results for nine liver-related indicators from 180 microliters of whole blood within 4 minutes. Validated with 103 clinical specimens, it shows high concordance with a clinical gold standard analyzer (Pearson coefficients >0.9). The EE-POCT platform not only advances liver health diagnostics but also sets a precedent for developing versatile, accessible medical technologies that can significantly affect global health outcomes.
研究领域:
生物医学工程
·
诊断技术
·
微流控技术
·
即时检测
·
临床化学
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产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Jie Ni,Jie Wei,Yeqi Yu,Ming-Hui Fan,Wanting Liu,Kwun Nam Hui,Yan Yan,Yan Liu,Yanqiang Huang,Jie Zeng
Hydroxylamine (NH2OH) is an important nitrogenous feedstock in the production of numerous valuable chemicals. The selective electroreduction of nitrate (NO3–) into NH2OH paves a promising avenue for the synthesis of NH2OH. Bi-based catalysts are regarded as the most promising candidates owing to the weak adsorption for NH2OH, preventing the further reduction of NH2OH. It is worth noting that Bi-based catalysts exhibited a dramatic activity gap in the acid and neutral/alkaline electrolytes toward the synthesis of NH2OH. Unfortunately, the mechanistic origin of pH-dependent NH2OH production has rarely been investigated, lacking an insightful interpretation on the atomic and molecular scale. Herein, taking Bi nanosheets (NSs) as a model catalyst, we investigated the underlying origin of the pH-dependent selectivity in NO3– electroreduction toward NH2OH production. The Faradaic efficiency (FE) for NH2OH of Bi NSs dramatically decreased from 86.6 to 12.0%, with the pH value ranging from 0 to 3. Combining the in situ Fourier transform infrared spectroscopy and theoretical calculations, we revealed that the dominant 4-coordinated hydrogen-bonded water in acid media favored the involvement of *H in the hydrogenation of *NO to *NHO, thereby facilitating the synthesis of NH2OH. Moreover, 10 kinds of amino acids were successfully synthesized by Bi NSs, demonstrating the wide universality of Bi nanosheets for the synthesis of amino acids.
研究领域:
电化学
·
催化
·
材料科学
·
表面科学
·
光谱学
·
理论化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiaorong Hao,Zhehao Sun,Chen Hu,Ziyi Wang,Kangning Wang,Shaokun Chong,Zongyou Yin,Zhengqing Liu,Wei Huang
Electrolyte additive engineering is crucial for advancing aqueous zinc-ion batteries (AZIBs), yet stereochemical effects remain underexplored. This study systematically investigates the impact of L- and D-configured amino acids as electrolyte additives, uncovering unprecedented chiral-dependent effects on Zn 2+ solvation and anode interfacial chemistry. Taking valine (Val) as a model additive, we reveal that L-Val forms a dual‑oxygen-coordinated solvation sheath with Zn 2+ , effectively reducing H 2 O activity and suppressing side reactions. In contrast, D-Val forms a mono‑oxygen-coordinated complex with limited solvation structure modification. Mechanistic insights show that L-Val preferentially adsorbs onto the Zn anode via its amino (−NH 2 ) functional group, while outward carboxyl (-COOH) groups self-assemble into a protective layer. This architecture promotes dendrite-free Zn deposition and reversibility, achieving symmetric cell cycling stability of 5200 h (vs. 3600 h for D-Val), Zn||Cu cell Coulombic efficiency of 99.7 % over 1500 cycles, and pouch cell capacity retention of 93.2 % after 1100 cycles. These findings highlight chiral molecular engineering as a transformative strategy for electrolyte design, offering generalizable principles applicable to multivalent-ion battery systems.
研究领域:
储能
·
电化学
·
材料科学
·
电池技术
·
分子工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Hanyu Yang,Hui Chen,Ling Jiang,Jie Hu,Xiupei Yang,Qian Zhang
The development of sensitive and selective detection methods for captopril (CAP), a widely used antihypertensive drug with potential environmental and health risks, remains an important challenge. In this work, we present a novel ratiometric fluorescence sensor based on folic acid (FA)-assisted β-cyclodextrin-coated copper nanoclusters (FA-Cu NCs@β-CD) for rapid CAP detection. The fluorescence of the Cu NCs capped with 2-mercaptobenzimidazole-5-sulfonic acid sodium salt (MBZS) was weak, but its intensity increased significantly upon encapsulation by β-CD, which is consistent with the mechanism of aggregation-induced emission (AIE). The addition of blue-emitting FA yielded a dual-emission fluorescent probe. CAP detection was achieved through its specific affinity for copper, which disrupted the structure of the Cu NCs, leading to quenching of the orange emission (605 nm) while maintaining the stability of the blue fluorescence (460 nm) of FA. The method exhibited a wide linear detection range (0.25–40 μg/mL) and a low limit of detection (61 ng/mL). The system demonstrated excellent selectivity against interfering substances and was successfully applied to detect CAP in river water and human urine samples, with good recoveries (97.5–107.0 %). Notably, the distinct color change from pink to blue after adding CAP allowed for smartphone-based visual quantification, demonstrating significant potential for onsite monitoring. This work provides a simple, cost-effective, and reliable approach for CAP detection that combines the advantages of ratiometric fluorescence with portable visualization.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物医学工程
·
药物分析
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）