MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Zhishan Fang,Shuyi Song,Shuo Zhang,Yuxuan Li,Mingshan Zheng,Xi-Ling Li,Ri Jin,Jun Zhe Min
A method for analyzing DL-thiol compounds in beer is established by NCS-OTPP. • The content of DL-thiol compounds are used to distinguish light and wheat beers. • The fitting curve reflect metabolic status of thiol compounds after drinking beer. • Provides novel methods for evaluating the quality of fermented foods. Thiol compounds are pivotal determinants of beer flavor and quality. However, conventional analytical approaches only enable the quantification of total racemic thiols, failing to achieve enantiomeric separation and targeted detection, which severely restricts the in-depth exploration of thiol-related flavor characteristics and biological activities. Herein, we developed and validated a UHPLC-HRMS strategy based on a self-synthesized chiral mass spectrometry probe, ( R )-(5-(3-isothiocyanatopyrrolidin-1-yl)-5-oxopentyl) triphenylphosphonium (NCS-OTPP), for the simultaneous enantioseparation and quantitative analysis of four chiral thiols (glutathione (GSH), homocysteine (Hcy), cysteine (Cys), N -acetylcysteine (NAC)) and γ-glutamylcysteine (γ-GluCys) in beer. Benefiting from the integrated advantages of permanent charge enhancement, specific chiral recognition, and thiol-targeted derivatization of NCS-OTPP, all diastereomeric derivatives were well baseline-separated on a conventional C18 reversed-phase column with resolution ranging from 1.52 to 1.71. The established method exhibits excellent analytical performance, including limits of detection of 2.50–14.40 fmol, intra-and inter-day RSDs of 1.45%–10.47%, and matrix-spiked recoveries of 98.55%–105.87%. Furthermore, PCA analysis based on the enantiomeric profiles of ten commercial beer samples (five wheat beers and five light beers) achieved precise classification of beer types, with L-Cys and D-Hcy confirmed as the core differential characteristic markers. In vivo metabolic monitoring revealed that urinary thiol enantiomers from healthy volunteers after beer ingestion presented a dynamic trend of “rapid surge-gradual decline” within 30–60 min. To demonstrate the rapid absorption, hepatic antioxidant metabolism and renal clearance characteristics of exogenous thiol compounds. Overall, this innovative chiral derivatization-MS platform provides a reliable technical support for beer flavor evaluation, quality traceability, and food bioactivity research, and offers a novel and feasible workflow for in-depth in vivo analysis of chiral thiol metabolites. Download: Download high-res image (132KB) Download: Download full-size image
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Nature Communications
·
2026
·
IF 18.1
Wang Xu,Li Shuangyu,Shi Wenzhuang,Xue Beiru,Wang Xu,Liu Miao,Lu Binjuan,Xu Zhouye,Gao Ye,Liu Jiachang,Ji Xingyue,Li Jia-Tang,Wang Yipeng
Antimicrobial peptides (AMPs) are essential components of the innate immune system in animals, playing crucial roles in defending against microbial infections. Hydrogen sulfide (H 2 S), a vital gasotransmitter, is involved in regulating a wide array of physiological functions. However, the role of H₂S in the anti-infective actions of AMPs remains poorly understood. Here, we demonstrate the crucial role of H 2 S in mediating the anti-infective functions of AMPs across diverse animal species. LL-37/CRAMP, the human cathelicidin family AMP and its mouse homolog, induce H 2 S production predominantly through 3-mercaptopyruvate sulfurtransferase (3MST) in Escherichia coli and Clostridium perfringens . The generated H₂S further participates in reactive oxygen species (ROS) accumulation, leading to bacterial death by oxidative damage. Additionally, LL-37 activates the Akt signaling pathway, upregulating cystathionine γ-lyase (CSE) expression to generate H 2 S in macrophages. This process plays a potential role in facilitating the immunomodulatory activity of LL-37/CRAMP and we confirmed that H 2 S is involved in their in vivo anti-infective activity of LL-37. Moreover, besides LL-37/CRAMP, we reveal that AMPs from various animal species also induce H 2 S production in bacteria and macrophages. Overall, our findings highlight a previously unrecognized role of H 2 S in AMP anti-infective function, which is ubiquitous across AMPs from different animals.
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RSC Advances
·
2022
·Vol. 12
·期 42
·27199-27205
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IF 4.036
Xuejing Wang,Yinyan Yuan,YiXiao Sun,Xue Liu,Mingze Ma,Renyin Zhang,Feng Shi
The development of luminescent materials greatly affects the development of fluorescence imaging technology. The preparation of carbon dots (CDs) with high photoluminescence quantum yield (PLQY) in the solid-state is challenging due to excessive resonance energy transfer (RET) and direct π–π interactions. In this study, we synthesized carbon dots that exhibit green fluorescence (GCDs) with absolute PLQYs up to 35.65% in one step by a microwave-assisted method. In the solid-state, the absolute PLQY reached 19.25%. Then, the GCDs were mixed with soluble starch in appropriate proportions, which improved the adsorption and dispersion of the GCDs and greatly reduced the cost of the fingerprint powder, and increased the absolute PLQY of the fingerprint powder to 41.75%. Finally, we prepared GCDs for preliminary fabrication of luminescent films, and the GCD-starch powder was successfully applied to high-quality latent fingerprint (LFP) imaging. The related properties of GCDs and the LFP detection performance of fingerprint detection powders prepared by GCDs were studied in detail. The results showed that the LFP system developed with GCDs-starch powder visualized LFPs with high definition and contrast under different conditions, and GCDs had potential for application in light-emitting devices. This study developed a new type of solid-state luminescent CDs and demonstrated that these GCDs have great application potential for LFP detection. This study may also provide inspiration for other applications based on efficient solid-state fluorescence.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
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IF 4.831
Ruihong Yao,Zhijian Li,Panpan Huo,Congcong Gong,Gang Liu,Chunhong Zheng,Shouzhi Pu
In this study, we constructed zinc based metal–organic framework functionalized by L-histidine (His@ZiF-8). The His@ZiF-8 had high porosity, large surface area, abundant carboxyl and amino group, which has been found to greatly enhance the aggregation-induced emission (AIE) of tetracycline (TC). After the His@ZiF-8 enrichment with TC, the TC exhibited strong fluorescence emission peak at 565 nm based on AIE. Under the optimal conditions, the fluorescence intensity of TC exhibited a good linear relationship with TC concentration within 0.1–80 μM with a low limit of detection of 28.6 nM. Antibiotic analogs such as neomycin, chloramphenico, ampicillin, kanamycin, and erythromycin have no obvious interference. In addition, the developed method was successfully used to the detection of TC in milk, river water, and honey.
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Bio-Design and Manufacturing
·
2026
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IF 7.9
Wang Yan,Wu Jin,Li Shicheng,Fan Rencai,Li Mingjun,Liu Xin,Yuan Cheng-song,Chang Yuanyuan,Ma Zequn,Guo Chunxian,Li Chang Ming,Chen Bo
Anoikis presents an alternative scheme for suppressing tumor metastasis. While limited by off-target effects, rapid degradation, and reactive oxygen species (ROS) accumulation, anoikis resistance remains a persistent challenge for current tumor therapy. The use of external and endogenous stimuli to trigger ROS bursts, as well as chirality-enhanced tumor microenvironment (TME) penetration, offers a valid solution. We developed a stimuli-responsive nanoreactor by coupling chiral histidine-modified manganese ferrite (MnFe 2 O 4 -PEG) nanoparticles with chiral cysteine-modified black phosphorus (BP) nanosheets via an ROS-sensitive thioketal (TK) bond to form a MnFe 2 O 4 -BP chiral magnetic nanoreactor (CT-MFBP). L-MFBP exhibited superior cellular uptake and intracellular retention due to its enhanced affinity for cell membranes. After entering the TME, the thioketal bond was cleaved by alternating magnetic field (AMF)-induced ROS, leading to the decomposition of the nanoreactor into chiral BP components. Subsequently, ROS accumulation was further amplified using BP-mediated photodynamic therapy (PDT). In addition, the nanoreactor demonstrated catalase (CAT)-like activity, converting endogenous H 2 O 2 into O 2 , thereby effectively alleviating the tumor hypoxic microenvironment and enhancing PDT efficacy. Furthermore, these events activated the mitogen-activated protein kinase (MAPK)-caspase 3 signaling pathway by upregulating p38 protein and Bcl-2-modifying factor (BMF) expression, which together promoted anoikis in tumor cells—a finding further validated by genomic analysis. Both in vitro and in vivo results confirmed that L-MFBP provides a robust regulatory strategy to induce anoikis and suppress tumor metastasis, offering a promising approach for chiral magnetic nanomedicine in clinical antitumor applications.
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Solar RRL
·
2023
·Vol. 7
·期 22
·2300547
·
IF 7.9
Zhiping Qian,Rui Zhang,Haijun Hu,Yan Xiao,Hui Li,Xiaodong Sun,Tianyi Ma
Zr–metal–organic frameworks (MOFs) have received much interest for their ultrahigh stability and are considered an up-and-coming class of catalysts for photocatalytic water splitting. However, their activity still needs to be improved. In this work, a series of defective UiO-66-NH 2 - x @CdS nanoparticles (NPs) ( x = 0, 50, 100, 150, 200, denotes the molar equivalent of the defect modulator) heterostructure photocatalysts is constructed for water splitting using defect engineering followed by a postmodification strategy. Defective structures are introduced to improve the photocatalytic activity of heterojunctions in the following ways: 1) modulating the energy band structure of UiO-66-NH 2 - x , 2) providing chelate binding sites for modifying the bridging molecules and thus building strong interactions between UiO-66-NH 2 - x and CdS NPs, and 3) serving as trapping sites to separate the photogenerated electron–hole pairs. Hence, this constructed series of UiO-66-NH 2 - x CdS NPs exhibit ultrahigh photocatalytic water splitting for H 2 production, especially UiO-66-NH 2 -150@CdS NPs with moderately defective levels showing catalytic activity up to 2303 μmol g −1 h −1 , which is 2.36 times higher than pure CdS NPs. Furthermore, the heterojunction catalysts with different defect levels exhibit a volcano-type trend, demonstrating the feasibility of defect engineering. This work provides novel insights for developing advanced defect-based MOF-constructed composite photocatalysts.
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ANALYTICA CHIMICA ACTA
·
2021
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IF 6.558
Shuting Chen,Weijia Wang,Shaohua Xu,Caili Fu,Shuyi Ji,Fang Luo,Cuiying Lin,Bin Qiu,Zhenyu Lin
In this work, an auto-identify sensor was constructed for rapid and high-precision detection of L-histidine. The proposed strategy is based on the auto-identify algorithm and the aggregation of alkynyl and azide functionalized gold nanoparticles induced by the Cu + catalyzed azides and alkynes cycloaddition (CuAAC) reaction. Specially, the color of scattering light spots for the aggregated gold nanoparticle (AuNPs) caused by CuAAC reaction was quite different from that of the monomers . However, L-histidine can bind to Cu 2+ and inhibits the production of Cu + , hence preventing the aggregation of AuNPs. Therefore, there is a distinct change of color as the addition of L-histidine under dark-field microscopy. Then, L-histidine can be quantitatively detected by combining the color change with the Meanshift algorithm accurately and automatically. Such proposed method has been successfully applied for the detection of L-histidine in serum sample with satisfying result.
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Journal of Analysis and Testing
·
2021
·Vol. 5
·期 1
·84-92
·
IF 0
Chen Shuting,Chen Chaoqun,Wang Jian,Luo Fang,Guo Longhua,Qiu Bin,Lin Zhenyu
In this work, a novel fluorescent biosensor has been constructed for rapid detection of Cu(II) via the interaction between the fluorophore groups on the surface of nitrogen-doped-carbon-dots (N-CDs) and ·OH produced from the catalytic reaction between Cu (II) and cysteine (Cys). Specifically, Cu (II) can catalyze the oxidation of Cys to form cystine (Cys–Cys) and hydrogen peroxide (H 2 O 2 ), and Cu (II) can also catalyze the decomposition of H 2 O 2 to produce hydroxyl radicals (·OH) by the Fenton-like reaction. ·OH can oxidize and destroy the surface structure of N-CDs, resulting in the fluorescence quenching of the N-CDs. Under the optimal experimental conditions, the linear range of Cu (II) is determined to be 0.05–25 μmol L −1 , and the limit of detection is 23 nmol L −1 with the limit of quantitation of 77 nmol L −1 . Besides, some characterizations are provided to verify the proposed principle. The method has been successfully applied for the detection of Cu (II) in human serum and environmental water with high sensitivity and higher selectivity.
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TALANTA
·
2019
·
IF 4.916
Cui Xu,Ruifang Guan,Duxia Cao,Kaerdun Liu,Qifeng Chen,Yunqiao Ding,Yun Yan
Aggregation dependence is a universal feature for fluorophores, ranging from classical planar fluorescent dyes displaying aggregation-caused quenching (ACQ) and propeller-shaped AIEgens that exhibit aggregation-induced emission (AIE). In this work, we report compounds emitters (CA-PDA) that display aggregation-independent fluorescence owing to the formation of robust intramolecular, rather than intermolecular hydrogen bonding . We achieved CA-PDA by synthesis of an amide from citric acid (CA) and propane diamine (PDA). Quantum chemistry calculations suggest the CA-PDA compounds have closed structures facilitated by intramolecular hydrogen bonds , which forces the lone pair electrons on oxygen and nitrogen to within 2.9 Å. Then the strong electron clustering generates a pseudo conjugated effect and leads to blue emission. This emission exhibits recoverable photobleaching, as UV irradiation only temporally breaks the intramolecular hydrogen bonds, which may form again upon relaxation. CA-PDA can be used in cell imaging as well as in probes for Hg 2+ and sulfur-containing amino acids. We expect that the strategy of a bioinspired intramolecular hydrogen bonds-facilitated compounds emitters will provide a new avenue toward advanced fluorescent materials.
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ANALYST
·
2020
·Vol. 145
·期 5
·1878-1884
·
IF 3.978
Jian Zhang,Yanqing Miao,Zhao Cheng,Lingling Liang,Xiaoya Ma,Chunye Liu
A fluorescent probe for the colorimetric detection of cysteine (Cys) was synthesized by combining resorufin with 7-nitrobenzofurazan. The resultant probe was characterized by FT-IR spectroscopy, fluorescence detection, mass spectrometry and NMR spectroscopy. After reacting with Cys, resorufin is released and the color changes from light yellow to red on paper-based analytical devices. The results were recorded using a common cell phone and subsequently analyzed using Photoshop software. The color intensity of the RGB channel increased linearly in the 0.04 to 70.04 μM Cys concentration range when the probe concentration was 2.6 mM (R = 0.9965), and the limit of detection was 16 nM. The effects of detection conditions have been investigated and are discussed. The interference deviations of 13 substances to Cys were in the range of −2% to +2%. The Cys concentration was determined as 270.8 ± 19.3 μM in human plasma, and the recovery was from 99.7% to 100.4%. The work demonstrates the potential of the method to detect Cys in real samples with low cost and high sensitivity.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Guo-Cheng Han,Xiaorui Su,Jiating Hou,Annaleizle Ferranco,Xiao-Zhen Feng,Ruosheng Zeng,Zhencheng Chen,Heinz-Bernhard Kraatz
The abnormal content of hemoglobin in human body can lead to several diseases such as anemia, thalassemia, leukemia, heart disease. Therefore, it is very important for sensitive detection of hemoglobin in clinical analysis. An electrochemical approach is presented for detecting hemoglobin using disposable electrochemical sensors, which were based on the ability of the redox active ferrocene group of Fc[CO-Cys(Trt)-OMe] 2 and Fc[CO-Glu-Cys-Gly-OH]. The two electroactive materials were adsorbed onto gold nanoparticles that were previously electrochemically deposited onto screen-printed electrodes. The as-prepared sensors were characterized by scanning electron microscopy and energy dispersive spectroscopy, and their electrochemical properties were characterized by cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. Results indicated the Fc[CO-Glu-Cys-Gly-OH] prepared sensor showed enhanced current responses for hemoglobin catalysis than the Fc[CO-Cys(Trt)-OMe] 2 prepared sensor. Optimal operating conditions were determined for hemoglobin detection by differential pulse voltammetry. The concentration of hemoglobin showed a good linear relationship with the current difference in the range of 0.1–1000 μg/mL and with 0.03 μg/mL limit of detection. The development of this electrochemical sensor was used for hemoglobin detection in human serum with a R.S.D. lower than 4.7% and recovery between 95.5% and 103.2%, which was in agreement with the data obtained from typical clinical analysis.
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BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jianping Li,Xionghui Ma,Mengxi Li,Yun Zhang
A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1–5 U/mL, with a detection limit of 0.028 U/mL (3δ/K), while the linear of the monosaccharide imprinted sensor was 1–60 U/mL and the detection limit was 0.17 U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor.
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