JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Yan Wang,Fuming Xue,Shuai Yu,Yan Cheng,Min Yin,Shichao Du,Junbo Gong
Cellulose derivatives such as hydroxyethyl cellulose (HEC), methylcellulose (MC) and hydroxypropyl methycellulose (HPMC) were employed to modify the morphology and growth of DL-methionine crystals. The methodology involved a systematic study through batch cooling crystallization, slow solvent evaporation, seeded single crystal growth, solid-state characterization, and molecular simulation. The presence of the polymeric additive, at very low concentrations (1–100 ppm), has modified the crystal shape from elongated leaf-like crystals to hexagonal plates or blocks with smaller aspect ratio. All three polymers exhibited strong retardation on crystal growth along the b -axis (length) and c -axis (width) direction. The growth inhibition degree depends on the types and concentrations of the additives, which can be ranked as HPMC > MC > HEC at same concentration. To further explore the mechanism of polymeric additives on morphology modulation and growth inhibition, Hirshfeld surface analysis, molecular electrostatic potential surface calculations, and molecular dynamic simulations of additive-crystal surface system as well as aqueous solution-crystal surface system were carried out. The molecular modeling revealed surface adsorption of additives on crystal faces via electrostatic interactions, van der Waals interactions, and hydrogen bonds. Other factors like hydrophobic interactions and steric hindrance effect of polymers also have significant impact on crystal growth inhibition.
研究领域:
化学
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材料科学
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DOI
高影响因子
JOURNAL OF ANIMAL SCIENCE
·
2020
·Vol. 98
·期 10
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IF 2.092
Sun Mingfa,Zhao Jingpeng,Wang Xiaojuan,Jiao Hongchao,Lin Hai
The supplementation of dietary limiting amino acids (AA) with crystalline AA makes the use of low-protein diets an option in poultry production. The differing absorption rates of crystalline and protein-bound AA may lead to temporally imbalanced AA in the postabsorptive period. In this study, two experiments were conducted to evaluate the effect of encapsulated L-lysine-HCl (L-Lys-HCl) and DL-methionine (DL-Met) on the laying performance of hens. In exp. 1, a total of 135 forty-seven-wk-old Hy-Line Brown hens were subjected to three dietary treatments for 8 wk: basal diet supplemented with 0.14% L-Lys-HCl and 0.17% DL-Met to satisfy the NRC (1994) total Lys and Met recommendation (control) and basal diet supplemented with encapsulated L-Lys-HCl and DL-Met at the levels of 60% (60CLM, 0.084% L-Lys-HCl and 0.102% DL-Met) or 80% of control (80CLM, 0.112% L-Lys-HCl and 0.136% DL-Met), respectively. In exp. 2, 24 fifty-five-wk-old Hy-Line Brown hens were individually reared in cages and subjected to the same treatments as in exp. 1. The plasma concentrations of free AA and nitrogen metabolites were measured 2, 4, and 6 h after fed. The results showed that dietary AA treatment had no significant influence on body weight (BW), feed intake, laying rate, egg weight, egg mass, or feed efficiency. The expression levels of AA transporters CAT-1, y+LAT1, b0,+AT, B0AT, rBAT, EAAT3, and PepT1 in the duodenum, jejunum, and ileum were not influenced (P > 0.05) by dietary treatment. There was an interaction of dietary AA treatment and time (P < 0.05) and the 80CLM hens exhibited higher concentrations of Lys (P < 0.05) than the controls at 2-h time point. In contrast, plasma Met concentration was not influenced (P > 0.05), while Cys was reduced in the 60CLM hens at every time point. The 80CLM hens had higher taurine concentrations than those receiving the control diet at every postprandial time point. In conclusion, these findings demonstrate that by using encapsulated form, the supplemental levels of synthetic L-Lys-HCl and DL-Met can be effectively reduced by approximately 20% with no negative effect on laying performance. The result suggests that encapsulated Lys and Met may ameliorate the postabsorptive AA balance and contribute to the reduced dietary AA supplemental levels.
研究领域:
动物营养学
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家禽科学
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氨基酸代谢
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Siqi Chen,Wei Tang,Huayan Luo,Qingqing Peng,Li Wang,Ruifeng Xie,Zhongmin Su,Fang Chai
Given the profound significance of tracing Fe 3 + in the environment and food, a fluorescent method for monitoring Fe 3+ by gold nanoclusters (MN-AuNCs) as the sensor was proposed in this work. The MN-AuNCs were prepared via a rapid procedure within 15 min utilizing DL-methionine (Met) and N-acetyl-L-cysteine (NAC) as dual ligands. Meanwhile, the introduction of NAC significantly enhanced the fluorescence quantum yield (QY=13.6 %), 1.5 times that of Met-AuNCs. The MN-AuNCs exhibited high sensitivity and selectivity towards Fe 3+ , with a detection limit of 83 nM. The portable intelligent method based on Smartphone RGB recognition was innovatively developed for rapid on-site monitoring of Fe 3+ . Notably, the MN-AuNCs also demonstrated significant antibacterial activity, with inhibition rates of up to 95 % against Escherichia coli and Staphylococcus aureus. This sensor integrates multifunctionality, including rapid preparation, highly sensitive detection and antibacterial action, offering a novel effective tool for serving food safety and human healthcare.
研究领域:
分析化学
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纳米技术
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食品科学
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环境监测
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生物医学工程
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微生物学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Yusha Huang,Longcheng Gong,Chengli Xie,Wenling Qin,Min Wang,Lianzhe Hu,Zhining Xia
Herein, a biomimetic approach is developed to improve the phosphatase-like catalytic activity of a metal–organic framework (MOF) nanozyme. Specifically, biomimetic Zn sites, featuring a coordination environment similar to that of natural phosphatase, were covalently anchored into UiO-66-NH 2 nanozyme through a step-by-step modification process. Compared with pristine UiO-66-NH 2 , the resultant 66-IS-Zn exhibited a 130-fold increase in K cat . Mechanism studies suggest that the anchoring of Zn sites can only enhance the catalytic performance of MOFs that possess intrinsic phosphatase-like activity, and the enhancement of catalytic activity strongly relies on the coordination environment of Zn sites, which involves the co-coordination of N and O with Zn. Besides the excellent phosphatase-like activity, 66-IS-Zn also exhibited enhanced intrinsic fluorescence emission and high phosphodiesterase-like catalytic activity. Consequently, 66-IS-Zn was used as the sensing unit to construct a three-channel sensor array for distinguishing organophosphorus pesticides (OPs). This sensor array can successfully distinguish six OPs and allow the quantification of profenofos and phoxim. Moreover, the binary mixtures of OPs and cherry tomato samples were precisely discriminated without any overlapping, indicating the reliability and practicability of the sensor array.
研究领域:
生物化学
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材料科学
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分析化学
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Minghui Jiang,Xiao Liu,Yurui Xu,Shiyu Li,Xiangju Liu,Xiaokai Niu,Lei Lu,Xinru Sun,Zhitian Xie,Ziming Wang,Su-ping Cui
Natural biobased materials, which can be obtained from crustacean exoskeletons, angelica sinensis, vanilla beans, amino acids, etc., have significant potential as eco-friendly corrosion inhibitors. In this study, several eco-friendly materials (chitosan (CTS), ferulic acid (FA), cysteine (Cys), and methionine (Met)) were proposed as corrosion inhibitors to improve the corrosion resistance of steel. Their inhibition effects and mechanisms in simulated concrete pore (SCP) solution were evaluated by electrochemical, surface measurements, quantum chemical (QC) calculations, and molecular dynamics (MD) simulations. The results showed that these inhibitors effectively improved the corrosion resistance of steel, especially for CTS (with a maximum inhibition efficiency of 92.6%). The adsorption of these inhibitors followed Langmuir and Freundlich adsorption isotherms, revealing the physical–chemical mixed adsorption modes. Surface characterization techniques revealed that the protective layers formed by these inhibitors could effectively inhibit the corrosion of the steel. QC calculations and MD simulations further confirmed the experimental results. Performance of cement mortar measurements revealed that these four inhibitors exhibited no effect on the workability of fresh mortar and improved the strength of mortar specimens, with a maximum increase in the compressive strength of 11.18%. This study is of great significance to improving the durability of reinforced concrete in the future.
研究领域:
材料科学
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电化学
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理论计算
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土木工程
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DOI
高影响因子
Advanced Therapeutics
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2025
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IF 2.6
Xinwei Zhang,Ziping Wang,Yu Bai,Yulun Hu,Qing Chen,Yueping Liu,Mingming Chang
Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory processes. RA, which is typically associated with the accumulation of hyperactive immune cells (HICs), particularly M1 proinflammatory macrophages, is accompanied by elevated levels of reactive oxygen species (ROS) and decreased pH in the synovial membranes of the joints. In this work, a nano-delivery system (MTX-Mn 3 O 4 @PDA) is designed that can deliver photothermal materials, ROS scavengers, and synovial fibroblast inhibitors at the joint site for the treatment of RA. After injecting MTX-Mn 3 O 4 @PDA into the inflamed site of RA, photothermal therapy is initiated by near-infrared (NIR) laser irradiation, which resulted in the death of activated inflammatory cells at the lesion site. While polydopamine (PDA) slowly dissociates under stimulation by a low pH microenvironment. Subsequently, excess ROS interacts with the Mn 3 O 4 nanozyme and the undissociated PDA nanozyme, promoting ROS clearance, while Methotrexate (MTX) inhibits the proliferation of synovial fibroblasts. Intra-articular injection of MTX-Mn 3 O 4 @PDA (7 mg kg −1 ) into collagen-induced arthritis mice demonstrated efficacy in reducing toe swelling and significantly alleviating synovial inflammation, bone erosion, and cartilage degeneration. This nano-delivery system has potential clinical applications for treating RA.
研究领域:
医学
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生物材料学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2024
·Vol. 47
·期 18
·e202400418
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IF 2.8
Zhiyu Zhang,Cheng Yang,Di Zhao,Yuanqing Zhao,Lixin Li,Zhonghua Li,Zhenqiang Zhang,Kai Hu
Abnormal levels of catecholamine (CA) neurotransmitters and their metabolites in biological fluids can lead to various neurological disorders. Herein, a boric acid-functionalized hypercrosslinked polymer was prepared and utilized as a sorbent for the dispersive solid-phase extraction of CAs and their metabolites in rat serum. By combination with a high-performance liquid chromatography-fluorescence detector, the extraction parameters for the seven target analytes were optimized. Under the optimal extraction condition, the methodology for the quantitative analysis of CAs and their metabolites in rat serum samples was established. The limits of detection and limits of quantification were found to be in the ranges of 0.010–0.015 and 0.033–0.050 ng/mL, respectively. The results demonstrated satisfactory recoveries, with values ranging from 88.02% to 113.27%, accompanied by relative standard deviations within the range of 2.69%–9.59%. In addition, the method showed good anti-interference ability (matrix effect ranged from 2.64% to 18.07%). The developed method was validated for the determination of CAs and their metabolites in normal and Alzheimer's disease model rats’ serum, which proved the promising application of the method for CAs neurotransmitter analysis in biological samples.
研究领域:
分析化学
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神经化学
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生物医学工程
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DOI
PubMed
高影响因子
TALANTA
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2025
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IF 6.1
Yingfei Hui,Hao Guo,Mingyue Wang,Liping Peng,Borong Ren,Yanyan Ma,Wu Yang
Glutathione (GSH) levels have been linked to aging and the pathogenesis of various diseases, highlighting the necessity for the development of sensitive analytical methods for GSH to facilitate disease diagnosis and treatment. In this study, we synthesized a novel core-shell material, UiO@TBTA, by in-situ growing TFPB-TAPA COF on UiO-66-NH 2 through a Schiff base reaction. The resulting composite capitalize on the advantages of both materials, demonstrating excellent stability, large specific surface area, and abundant active functional groups while preserving superior crystallinity. Notably, this strategy effectively reduces the occurrence of aggregation-caused quenching (ACQ) in COFs. Due to the inner filter effect and hydrogen bonding interactions between UiO@TBTA and GSH, a specific ratiometric fluorescence detection of GSH was achieved in the range of 0.1–7 μM, with a limit of detection (LOD) of 0.0685 μM. In addition, due to the sensitive color change of the sensing material from orange to black caused by GSH, a proportional colorimetric sensing strategy has also been proposed, enabling the detection of GSH within the range of 1–200 μM. What's more, two intelligent artificial neural networks models were constructed with the help of machine learning that can quickly, accurately, and sensitively determine the concentration of GSH based on fluorescence images and color photographs respectively. Our work represents the first study utilizing MOF@COF composite for the multimodal detection of GSH, thus providing a novel strategy for the multimodal detection of the target analyte. Prospectively, the construction of the fluorescence/colorimetric dual-mode intelligent ratiometric sensing platform using deep machine learning holds great promise for real-time monitoring.
研究领域:
分析化学
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生物医学
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材料科学
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DOI
PubMed
高影响因子
Plasmonics
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2025
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IF 4.3
Chen Kun,Ma Chaoqun,Chen Guoqing,Yang Taiqun,Gao Hui,Li Lei,Hu Anqi,Cao Jun,Zheng Chenkai,Ma Longyao,Chen Zehao
The detection of pefloxacin (PEF) in milk is of significant importance for food safety. Traditional methods for detecting PEF often rely on techniques such as high-performance liquid chromatography (HPLC), which are complex and time-consuming. This article introduces a surface-enhanced Raman scattering (SERS) substrate based on a composite of covalent organic frameworks (COFs) and silver nanoparticles (AgNPs) for the rapid detection of PEF in milk. After testing PEF in both water and milk, the detection limits were calculated to be 6.31 μg/L for water and 82 μg/L for milk, with correlation coefficients of 0.991 and 0.998, respectively. Furthermore, the average recovery rate of PEF in milk ranged from 96.25% to 106.17%, with a relative standard deviation (RSD) of 4.32% to 8.88%. This work offers a simple and rapid method for detecting PEF in milk. Graphical
研究领域:
食品安全
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分析化学
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药物残留检测
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DOI
高影响因子
Biomass Conversion and Biorefinery
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2025
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IF 4.1
Zhao Xiaoben,He Tao,Wang Shuainan,He Song
In this research, nitrogen-doped activated carbons (NACs) featuring a high specific surface area (3396 m 2 /g) and a certain amount of nitrogen doping (0.79–1.69%) were prepared by a combination of chemical activation and self-activation methods, and their CO 2 adsorption characteristics were comprehensively assessed. The experimental results showed that nitrogen doping not only significantly enhanced the specific surface area of the materials, but also significantly enhanced the CO 2 adsorption. The CO 2 adsorption capacity of the optimal sample, CMAC-0.5–3-700, reached 4.11 mmol/g and 2.32 mmol/g at 0 °C and 25 °C, respectively. In addition, the selectivity of NACs for CO 2 /N 2 was significantly improved, with a selectivity factor of up to 50.16, and the competitive adsorption experiments also demonstrated that NACs could still effectively adsorb CO 2 under the condition of CO 2 /N 2 = 15/85. Density-functional theory (DFT) calculations further revealed the interaction mechanism between NACs and CO 2 molecules, the enhanced CO 2 selectivity is attributed to electrostatic and π-electron interactions between nitrogen atoms and CO 2 . The findings indicate the significant potential of nitrogen-doped activated carbons (NACs) with high specific surface area and substantial nitrogen doping for post-combustion CO 2 capture.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Wenli Zhu,Miao Hu,Guanghua Su,Weifang Jiang,Hancong Du,Kaxi Chen,Jinfang Nie,Lang Zhang,Xuehui Tang,Yun Zhang
This work initially describes a new colorimetric nanosensor for the visual detection of alkaline phosphatase (ALP) based on analyte-mediated in-situ production of silver nanoparticles (AgNPs) that lights up the Tyndall effect (TE). In the presence of ALP, 4-aminophenol phosphate (APP) is hydrolyzed to generate 4-aminophenol (AP), which in turn reduces Ag + ions to form AgNPs. The TE signal can be observed with the naked eye in the resulting colloidal mixture under the irradiation of a handheld laser pointer pen (acting as a light source). The TE intensity is proportional to the analyte concentration in the sample. The quantitative measurement of ALP is further realized by using a smartphone for reading out the TE signal. The results obtained under optimal experimental conditions show that the proposed nanosensor is capable of quantifying ALP within a concentration range of 0.0156–8 U/L. Its detection limit for the analyte is estimated to be approximately 0.002 U/L according to the 3σ rule. Moreover, the recovery of ALP in real human serum samples ranges from 94.38 to 96.67 %, demonstrating its satisfactory analytical accuracy and practicality. To our knowledge, this may be the first report of a nanoprobe-free TE-enhanced colorimetric nanosensor for the instrument-free analysis of enzymatic activity.
研究领域:
生物传感
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分析化学
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医学诊断
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-13
·
IF 5.3
Zhou Mengyan,Zhang Zhihui,Zheng Qi,Yu Mingdi,Si Mengxu,Wu Sirui,Zhang Yanan,Ding Shushu,Fu Ding-Yi
A novel ratiometric fluorescent nanohybrid probe was constructed for sensitive and selective determination of folic acid (FA) based on blue D-penicillamine-based carbon dots (CDs) and red glutathione S-transferase-Au nanoclusters (GST-AuNCs). Upon the excitation of 380 nm, the obtained CDs-NCs possessed two distinct emission peaks at 465 and 665 nm. The fluorescence intensity at 465 nm was incrementally enhanced with the addition of FA attributed to the hydrogen bonds formation, while the fluorescence intensity at 665 nm was quenched caused by the electronic interaction and the inner filter effects. The fluorescence intensity ratio ( I 465 /I 665 ) exhibited good linear correlation with FA concentrations in the range 10∼90 μM, and the limit of detection (LOD) was 0.63 μM. Notably, the corresponding fluorescent color changed from red to blue, which could be distinguished by naked eyes. On account of the excellent biocompatibility, the CDs-NCs were further successfully used for bioimaging and intracellular FA detection. Furthermore, the real sample analyses confirmed that the proposed nanoprobe could be expanded as a versatile platform for FA detection in practical applications. Graphical abstract
研究领域:
分析化学
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生物医学工程
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纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）