NUTRITION RESEARCH
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2026
·
IF 3.6
Ningli Ye,Minghui Yu,Haixin Ju,Yuanyan Jiang,Yumeng Zhang,Lei Sun,Xiaohui Wu,Jing Chen,Hong Xu,Qian Shen
Imbalance of maternal diets can impair offspring development and contribute to congenital anomalies of the kidney and urinary tract (CAKUT). Excess maternal methionine intake is known to impair fetal development. The placenta serves as a crucial interface for maternal–fetal exchange and may contribute to these adverse outcomes. We hypothesize that excess methionine intake during pregnancy in mice increases the incidence of CAKUT in offspring. Therefore, we established a mouse model of 10% (w/w) methionine intake during pregnancy from Embryonic Day 8.5 to11.5 (E8.5-E11.5) and assessed the kidney phenotype. Crucial kidney developmental pathways were analyzed by RT–qPCR and immunofluorescence at E11.5. Changes in methionine metabolism in maternal plasma and placental tissues at E11.5 were analyzed using liquid chromatography–tandem mass spectrometry, and placental RNA–seq was performed. Offspring from dams exposed to excess methionine exhibited increased incidence of CAKUT (relative risk = 4.15, 95% confidence interval: 1.40-12.34, P = .010), primarily presenting as hydronephrosis and/or duplex collecting system. E11.5 embryonic kidneys showed shorter common nephric duct and decreased activity of the Gdnf/Ret/p-Akt pathway. At E11.5, homocysteine was elevated in the placentas, together with decreased mRNA expression of key metabolic enzymes betaine-homocysteine S-methyltransferase (Bhmt) and cystathionine γ-lyase (Cth) in the placentas. Concurrently, RNA-seq of E11.5 placentas revealed alterations in immune-inflammatory and vascular-related pathways. Overall, excessive maternal methionine intake in mice increased the incidence of CAKUT in offspring, which may be associated with altered activity of the Gdnf/Ret/PI3K/p-Akt pathway in embryonic kidneys as well as elevated placental homocysteine.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 40
·19056-19064
·
IF 3.591
Xiaochun Deng,Jingwen Zhao,Yao Ding,Hongliang Tang,Fengna Xi
As an alternative to natural enzymes, peroxidase nanozymes have shown great potential in the fields of sensing, drug delivery, and tumor treatment. However, obtaining peroxidase nanozymes with high catalytic activity, simple synthesis, and low cost is still a huge challenge. Herein, we demonstrate facile synthesis of iron and nitrogen co-doped graphene quantum dots (Fe,N-GQDs), which possess high peroxidase activity. With hemin being a precursor, Fe,N-GQDs can be easily synthesized with a high production yield (74%) using a one-step hydrothermal process. The as-prepared Fe,N-GQDs exhibit uniform size (2.9 nm), good water dispersibility and bright yellow-green fluorescence (an absolute fluorescence quantum yield of 12.5%). In particular, Fe,N-GQDs exhibit very high peroxidase-mimicking activity and can rapidly oxidize peroxidase substrates 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxidized TMB (oxTMB) in the presence of H2O2. In comparison with a natural enzyme horseradish peroxidase (HRP), the Fe,N-GQD nanozyme has a lower Michaelis constant (Km) and a higher maximum reaction rate (Vmax), indicating higher substrate affinity and faster reaction rate. Selective and sensitive colorimetric detection of L-cysteine (L-Cys) is realized in terms of its ability to reduce oxTMB and to react with H2O2. The linear range for the detection is 0.5–50 μM with a limit of detection (LOD) of 140 nM. In addition, the detection of L-Cys in compound amino acid tablets is also realized.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Chengcheng Li,Xiaodong Zhang,Yuxin Guo,Farzad Seidi,Xiaotong Shi,Huining Xiao
Naturally occurring nanoscale exopolysaccharide (EPS) has attracted much attention in recent years. In this research, we obtained a new kind of naturally occurring spherical EPS nanoparticles (EPS-R503) from Lactobacillus plantarum R503. The secretion, self-assembly process, morphological structure, and surface characteristics of the as-prepared nanoparticles were comprehensively revealed with transmission electron microscopy (TEM) and atomic force microscope (AFM) for the first time. It was found that the EPS-R503 nanoparticles consist of negatively charged heteropolysaccharide composed of mannose, glucose, galactose, and glucuronide with several functional groups including −OH, −COOH, and −NH2. When different solvents were used to treat the EPS-R503 nanoparticles, the morphological structure and surface properties could be changed or manipulated. The forming mechanism of EPS-R503 was elucidated based on the aggregation processes from a fundamental point of view. Furthermore, EPS-R503 can serve as reducing and stabilizing agents for the biosynthesis of manganese dioxide nanosheets (MnO2 NSs), leading to EPS-MnO2 nanocomposite. The as-prepared nanocomposites can absorb fluorescein (FL) to form EPS-MnO2-FL, which can be used to detect glutathione (GSH) with a low limit of detection (0.16 μM) and a wide detection range from 0.05 to 4 mM. The excellent biocompatibility of EPS-MnO2-FL endows the feasibility of in vivo detection of GSH as well. Overall, the findings from this work not only benefit the exploitation of naturally occurring EPS nanomaterials but also provide a novel strategy for the green synthesis of metal-containing nanosheets for GSH detection.
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高影响因子
ANALYST
·
2020
·Vol. 145
·期 5
·1878-1884
·
IF 3.978
Jian Zhang,Yanqing Miao,Zhao Cheng,Lingling Liang,Xiaoya Ma,Chunye Liu
A fluorescent probe for the colorimetric detection of cysteine (Cys) was synthesized by combining resorufin with 7-nitrobenzofurazan. The resultant probe was characterized by FT-IR spectroscopy, fluorescence detection, mass spectrometry and NMR spectroscopy. After reacting with Cys, resorufin is released and the color changes from light yellow to red on paper-based analytical devices. The results were recorded using a common cell phone and subsequently analyzed using Photoshop software. The color intensity of the RGB channel increased linearly in the 0.04 to 70.04 μM Cys concentration range when the probe concentration was 2.6 mM (R = 0.9965), and the limit of detection was 16 nM. The effects of detection conditions have been investigated and are discussed. The interference deviations of 13 substances to Cys were in the range of −2% to +2%. The Cys concentration was determined as 270.8 ± 19.3 μM in human plasma, and the recovery was from 99.7% to 100.4%. The work demonstrates the potential of the method to detect Cys in real samples with low cost and high sensitivity.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-9
·
IF 5.479
Sun Jiangjiang,Wang Qin,Yang Jiajun,Zhang Jianjian,Li Zheng,Li Hua,Yang Xiao-Feng
The authors describe a carbon dot-based fluorescent probe for biothiols. Green emissive carbon dots (g-CDs; with λ ex /λ em maxima of 407/505 nm) were synthesized by a one-step solvothermal method starting from 3-diethylaminophenol. They were then covalently functionalized with 2,4-dinitrobenzenesulfonyl chloride to afford 2,4-Dinitrobenzenesulfonate-functionalized CDs (g-CD-DNBS) as a nanoprobe for biothiols. The fluorescence of the g-CD-DNBS is quite weak. Upon addition of biothiols, the DNBS group of the probe is removed by thiol groups. This results in gradual restoration of the green fluorescence. The nanoprobe exhibits high selectivity for biothiols over other amino acids and biological molecules. The detection limits for cysteine, homocysteine and glutathione are 69, 74 and 69 nM (S/ N = 3), respectively. The probe was applied to image biothiols in SMMC-7721 cells.
Schematic presentation of the mechanism of 2,4-dinitrobenzenesulfonate-functionalized carbon dots (g-CD-DNBS) for the detection of biothiols. g-CDs: green emissive carbon dots.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Yuxin Guo,Xiaodong Zhang,Fu-Gen Wu
Reduced glutathione (GSH) is a crucial endogenous antioxidant that plays a central role in maintaining cell activity by involving in cellular defense against reactive oxygen species (ROS). Therefore, the detection of intracellular GSH is essential to monitor the cellular state. Herein, graphene oxide–MnO 2 –fluorescein (GO–MnO 2 –FL) nanocomposite was synthesized and used for GSH detection. GSH could reduce MnO 2 to Mn 2+ and block the energy transfer between FL and GO–MnO 2 , leading to the significant fluorescence enhancement of FL. The fluorescence-based GSH sensing has a limit of detection of 1.53 μM and a wide detection range of 10 μM–2 mM. Moreover, the GO–MnO 2 –FL nanocomposite was found to selectively image the cancer cells since the GSH content of cancer cells is higher than that of normal cells. In vivo experiments also confirmed the normal/cancer cell differentiation capability and excellent tumor-targeting ability of the GO–MnO 2 –FL nanocomposite. These properties make the GO–MnO 2 –FL nanocomposite an excellent fluorescent probe for GSH detection both in vitro and in vivo , and we believe it may hold great potential for GSH-mediated cancer diagnosis.
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高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Yuanlin Huang,Tianran Lin,Li Hou,Fanggui Ye,Shulin Zhao
Based on Fenton reaction without adding nanomaterials , a simple colorimetric sensor for detecting thioglycolic acid (TGA) was developed. By reducing Fe 3+ ions to Fe 2+ ions, TGA efficiently stimulates Fenton reaction through accelerating Fe 3+ /Fe 2+ redox cycle. Owing to this, the accelerated decomposition of H 2 O 2 generates more OH radicals, which cause a significant blue change in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Compared with Fe 3+ /H 2 O 2 /TMB system, this sensor can be operated over a wider pH range from 4.0 to 8.0. The calibration curve of TGA is achieved in the concentration range of 0.046–7.37 μg mL −1 under optimized conditions, and the detection limit of TGA is 0.036 μg mL −1 (S/N = 3). This sensor has been successfully applied to the detection of TGA in cold wave lotion with recovery rates between 99.1 and 101.6%, which has broad application prospects in cosmetic analysis and environmental monitoring.
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CARBON
·
2016
·
IF 6.198
Fang Gao,Shiyao Ma,Jun Li,Kang Dai,Xincai Xiao,Dan Zhao,Weifan Gong
A green pyrolysis approach to prepare ultra-small nitrogen and sulfur-co-doped carbon dots (N,S-C-dots, QY reaches 55.7%) with precursors citric acid (CA) as carbon source and N-acetyl- l -cysteine as N and S dopant has been explored. The optical properties and the origin of photoluminescence (PL) have been investigated at length. The mixed excitation independence character (200 ≤ λ ex ≤ 380 nm) and the excitation dependence feature ( λ ex > 380 nm) along with the biexponential behavior of lifetime of prepared C-dots all indicate the existence of two different emissive sites: the surface state caused by the introducing of N and S on C-dots surfaces and the molecular state induced by the fluorophores covalently bonded onto C-dots surfaces. More importantly, for the first time, through comprehensively comparing the PL performances of various types of N,S-C-dots, N-C-dots and S-C-dots synthesized under same condition, we report the efficient chemical structure motif of reactant, which could form small fluorophore molecules with high PL during the synthesis and thus exhibits favorable impact on PL performances of resulting C-dots. Detailed structural and property studies suggest that the chemical structure motif of reactant ( HN C C SH) determines the optical properties of CA-derived C-dots, which would be valuable in selecting efficient reactants for preparing highly fluorescent CA-derived C-dots.
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THIN SOLID FILMS
·
2014
·
IF 1.867
Xiangying Sun,Bin Liu,Qiong Wu,Fang Li
In the present work, the properties of triptycene orthoquinone derivatives were studied. As a kind of good electron-transfer platform, triptycene derivatives with different electron donors or electron acceptors behave distinctively with their luminescent properties. The intensity ratio of fluorescence peaks can be controlled by the number of methoxy groups (electron donor) and orthoquinone groups (electron acceptor) simultaneously. We have assembled 6,7,12,13-4-methoxyl-2, 3-2-orthoquinone triptycene onto self-assembled monolayers (SAMs) to create a probe for detecting biological thiols. The SAMs exhibited higher selectivity toward homocysteine than to other thiol-containing compounds with a fast response and a stable signal over a wide liner range from 2.0 μmol/L to 1.0 mmol/L with the detection limit of 0.52 μmol/L.
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危险品化学品经营许可证（带存储）