SENSORS
·
2018
·Vol. 18
·期 8
·2679
·
IF 2.475
Zheng-Jun Xie,Xian-Yu Bao,Chi-Fang Peng
A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+by centrifugation. It was found that the CH3Hg+reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+detection in environmental samples.Keywords:methylmercury;gold nanoparticles;enzyme mimic;chromogenic reaction
研究领域:
环境科学
·
纳米生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 43
·16388-16396
·
IF 8.067
Wenrui Ma,Lulu Liu,Xu Zhang,Xingfei Liu,Yi Xu,Shunbo Li,Muling Zeng
Artificial membranes with asymmetric nanopores are similar to biological membranes with ion channels. The selective and rectified ion transport property of artificial membranes is of great significance for developing electrochemical sensors with highly sensitive detection performance. Herein, we propose a fast and facile approach for the sensitive detection of metal ions or charged molecules by constructing an asymmetric nanochannel structure using a cation-selective Nafion membrane and a DNA-functionalized anodic aluminum oxide (AAO) membrane. Arising from the varying charge distributions induced by the recognition of targets by DNA probes, the Nafion/AAO heterostructured nanochannels exhibit high ionic current rectification and excellent Hg2+ regulated current responses. The present sensor with heterostructured nanochannels can realize the detection of Hg2+ with a low detection limit of 1 pM, due to the unique ionic transport property of asymmetric nanochannels and the sensitive electrochemical detection method. The sensing mechanism is also confirmed by numerical simulations, demonstrating that the differences in the rectification ratios in the heterostructured nanochannels are caused by the varying surface charge densities in AAO nanochannels. This simple and facile heterostructured sensor has the advantages of simple preparation, easy modification, simple instrumentation and high sensitivity, which possesses great potential in the detection of metal ions or charged molecules.
研究领域:
生物传感器
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电化学检测
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纳米技术
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分析化学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Han Dong,Letian Zhong,Yuliang Cheng,Hang Yu,Yunfei Xie,Weirong Yao,Yahui Guo,Hideya Kawasaki
The freezing method used to prepare DNA-modified gold nanoparticles (SNA) has the advantages of being fast and simple, but the prepared SNA is easy to aggregate. In this paper, the effects of polyethylene glycol (PEG) as an organic macromolecular crowding agent on the single-strand polyadenylate DNA functionalized gold nanoparticles (polyA-SNA) and the single-strand thiolated DNA functionalized gold nanoparticles (HS-SNA) were systematically studied. The experimental results showed the molecular weight and concentration of PEG significantly affect the dispersion and DNA loading of the two kinds of SNA . An increase in DNA strand length inhibits the adsorption of thiolated DNA and polyA-DNA, and the hydrated particle size of SNA increases at first and then decreases with the length of the DNA strand. These phenomena lay on the speculative principle that PEG improved the energy barrier for gold nanoparticles (AuNPs) aggregation through the long chain of macromolecules , and the modification mode and length of surface DNA also play an auxiliary role. This work provided a systematic study and helpful results on improving the stability of SNA prepared by the freezing method.
研究领域:
生物化学
·
纳米医学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 36
·3598-3604
·
IF 3.532
Wenxiu Huang,Lizhen Zhao,Ruidi Shen,Gongke Li,Liansheng Ling
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs–DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23–4.50 mg L−1, with a detection limit of 0.14 mg L−1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
研究领域:
生物化学
·
环境科学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Guobin Mao,Qin Cai,Fubing Wang,Changliang Luo,Xinghu Ji,Zhike He
As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of H2O2 and glucose. Compared with the traditional multistep construction of ratiometric fluorescent probes, this presented approach is simpler and more effective without chemical modification and complicated separation. The CdZnTeS QDs with green fluorescence is specifically sensitive to H2O2, while the red fluorescence of Rox is invariable. H2O2 is the product from the oxidation of glucose catalyzed by glucose oxidase (GOx). Therefore, a facile method to detect H2O2 and glucose with a detection limit of 0.075 μM for H2O2 and 0.042 μM for glucose was developed. In addition, this proposed probe has been employed for the detection of glucose in human serum with a satisfactory result. Moreover, this probe has been used for visual detection, and the health and diabetics can be distinguished by the naked eye. Meanwhile, this nanoprobe is also generalizable and can be extended to the detection of many other H2O2-mediated analytes.
研究领域:
生物化学
·
医学诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Qianlong Wang,Lancong Liu,Xiaoyi Chen,Tiantian Wang,Hua Zhou,Hui Huang,Linsen Qing,Pei Luo
Urine is the most appropriate body fluid for analysis because it is easily and less-invasively obtained than blood; thus, urinary miRNAs can better represent the local stage of the disease and might grow up to be a new class of noninvasive biomarkers of postmyocardial infarction (MI). Monofunctionalized Au nanoparticles (AuNPs) with only one selective DNA at a specific location are more promising in nanotechnology. This study developed a urinary miRNA ultratrace detection strategy based on single-target DNA-functionalized AuNPs for the noninvasive prognosis of post-MI. The AuNPs were designed with only single-stranded biotinylated DNA complementary to the target miRNA through a ratio-optimized stoichiometric method for the first time. Combined with the duplex specific nuclease-assisted target recycling amplification, the single-target DNA-functionalized AuNPs for the first time were used in inductively coupled plasma–mass spectrometry for the determination of urinary miRNA with high sensitivity. After optimizing the reaction conditions, a linear detection range between 1 fM and 10 pM for miR-155 and a detection limit of 0.47 fM were obtained. Finally, the target miR-155 in urine samples collected from MI rats was quantified and the level of miR-155 in MI groups was 30 times higher than in the control groups. The results suggest that urinary miR-155 could be a novel biomarker for the noninvasive diagnosis of MI.
研究领域:
分子生物学
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纳米医学
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临床诊断学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 33
·18728-18733
·
IF 3.049
Jinjin Yin,Jiuchao Wang,Xiyue Yang,Tao Wu,Huashan Wang,Xiaoming Zhou
In this work, a facile and sensitive colorimetric sensor for Hg2+ ions based on poly (adenine)-mediated DNA-functionalized gold nanoparticles (Au NPs) is reported. One DNA sequence consisting of poly-A and T-rich DNA was designed rationally. Poly-A was used as an anchoring block to bind tightly to Au NPs, and T-rich DNA was utilized for specific recognition of Hg2+ ions. With the assistance of poly-A, T-rich DNA was easily introduced onto the surface of Au NPs and kept an upright orientation. In the presence of Hg2+ ions, T base binding with Hg2+ ions results in the formation of “T–Hg2+–T” among the Au NPs, which caused aggregation of the Au NPs and a subsequent change in the color of the solution, from wine red to grayish blue. On this occasion, the limit of detection (LOD) was 3.75 nM Hg2+ ions with a linear range from 5 nM to 200 nM, as measured by UV-Vis spectroscopy. Moreover, successful application of this method for the detection of Hg2+ ions in real samples was demonstrated.
研究领域:
分析化学
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环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jianyang Lu,Minghui Wang,Yiwei Han,Ying Deng,Yujing Zeng,Chao Li,Jie Yang,Genxi Li
The functionalization of covalent organic frameworks (COFs) with biomacromolecules can extend their functions, which is the premise of their application in biomedical research. However, strategies to functionalize COFs with biomacromolecules, which can ensure the stability in complex medium and minimize the undesired effects, are still lacking. In this work, we have proposed a strategy to functionalize COFs with DNA by covalently linking DNA to the functional group on the COF surface through Cu(I)-catalyzed azide/alkyne cycloaddition (CuAAC) reaction. The as-prepared DNA-functionalized COFs (DNA-COFs) can exhibit good hybridization ability and cargo loading ability; thus, we have designed a DNA-COF-based nanoprobe and then fabricated an electrochemical biosensor for the detection of exosomes. In this design, the functionalization with DNA enables COFs to recognize and capture exosomes, and the encapsulation of a large number of methylene blue (MB) in COFs facilitates signal amplification, which can enhance the sensitivity of the biosensor. Moreover, by simply replacing the oligonucleotide sequences, the strategy proposed here can generally be used to build different DNA-COFs with diverse functions for broader biomedical applications.
研究领域:
生物医学工程
·
材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Bao-Kang Jin,Jiajia Wang,Chun Kan
In this paper, a proportional electrochemical biosensor based on cadmium telluride/ polypyrrole (CdTe/PPy) nanocomposite is developed for the detection of MUC1 with high selectivity and sensitivity. The result indicates that CdTe/PPy nanocomposite is modified on the surface of glassy carbon electrode (GCE), which supplies a large surface area for immobilizing cap-DNA, ensuring its high selectivity and sensitivity. Whereafter, CdTe linked sig-DNA (MUC1 aptamer) is introduced, allowing MUC1 aptamer to hybridize with cap- DNA. CdTe as signal amplification elements in order to generate the differential pulse voltammetry (DPV) signal. Conceivably, the target MUC1 detection was based upon the current signal change due to the quantities change of the signal amplification elements CdTe. In the presence of MUC1, the MUC1 aptamer specifically binds to MUC1, resulting in the release of CdTe-sig-DNA from the electrode surface and the decrease of peak current. Under the optimized experimental conditions, the electrochemical biosensor present a highly selective, sensitive, stability and reproducibility for the MUC1 from the range of 0.1 nM to 100 nM with detection limit of 0.05 nM (S/N = 3). Therefore, the electrochemical biosensor has a potential application in medical disease diagnosis application detection.
研究领域:
生物化学
·
纳米技术
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yue Huang,Jiehua Ma,Yuting Yan,Chao Li
Silicon, as the second richest element in the earth, is of great importance to the current nanoscience. Functionalization of silica nanomaterials with DNA molecules can significantly broaden their biological application. However, current assembly methods usually involve complex and time-consuming steps or detrimental reagents, limiting their real application. In this work, we report a metal ion-assistant DNA assembly method to simply, rapidly and robustly immobilize short DNA molecules on the surface of silica nanoparticles (SiNPs) and use it to perform different tasks. By fully screening the metal ions, metal ions such as Zn 2+ , Al 3+ , Y 3+ and Zr 4+ , are found to be effective on the mediation of the interaction between DNA and SiNPs. The DNA density can be finely tuned by adjusting the added concentration of DNA or metal ions, and the adsorbed DNA is highly stable in the metal ion-containing buffer. Through simply adjusting the kind of metal ions and DNA, different biological applications such as biosensing, purification, and gene regulation can be achieved. This work not only offers fundamental insights into the biointerface science of SiNPs with DNA, but holds great potential in the area of biomolecule purification, nucleic acid delivery, biosensing, and bioimaging.
研究领域:
纳米技术
·
生物技术
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Qing Kang,Tong Lin,Jingbo Jiao,Yidi Liu,Yurong Guo,Chenjing Ma,Xinjun Du,Shuo Wang
Salmonella typhimurium ( S. typhimurium ) contamination has become the main source of foodborne diseases and has substantial effects on human well-being. Herein, we present a nucleic acid-responsive colorimetric biosensor for ultrasensitive detection of S. typhimurium , integrating a multifunctional Fe 3 O 4 @Zr-MOF/GOx nanoprobe with exponential amplification reaction (EXPAR). The system integrates glucose oxidase (GOx) encapsulated within a Zr-based metal-organic framework (Zr-MOF) modified with magnetic Fe 3 O 4 nanoparticles. In the presence of S. typhimurium , the EXPAR reaction is triggered, and the controlled release of GOx is achieved by DNA strand displacement. The detection mechanism leverages GOx-catalyzed glucose oxidation to generate H 2 O 2 , which oxidizes 3,3’,5,5’-tetramethylbenzidine (TMB) for colorimetric signal readout. The biosensor demonstrates high specificity for S. typhimurium , with a detection limit of 2.26 CFU mL⁻¹ , and practical applicability in spiked milk samples, achieving recoveries of 89.6–111.5 %. The strategy employing stimuli-responsive DNA/MOF composites demonstrated significant potential in food quality monitoring and analysis.
研究领域:
微生物学
·
食品安全
·
生物传感器
·
分子诊断
·
纳米生物技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Chunying Li,Chunying Wei
We have developed a label-free fluorescent probe using DNA-templated silver nanoclusters (Ag NCs). The DNA-Ag NCs probe was a simple, non-toxic, and sensitive method and could be used to detect biothiols and the activity of acetylcholinesterase (AChE). The fluorescent intensity of DNA-Ag NCs was quenched significantly in the prescence of biothiols, which might be attributed to the formed nonfluorescent complex between Ag NCs and biothiols, the aggregation of Ag NCs, and the efficient energy transfer from DNA-Ag NCs to thiol group. The cysteine (Cys) and glutathione (GSH) could be detected as low as 0.134 μM and 0.172 μM, respectively, within a good linear range from 0–5.0 μM ( R 2 = 0.991) for Cys, and 0.5–4.5 μM ( R 2 = 0.983) for GSH. In addition, the proposed method could also detect selectively the activity of AChE in the range from 0–4 U/L, with a detection limit of 0.071 U/L. We believed that the proposed approach laid a foundation for the researchers in bioimaging, pharmacology, diagnostics, and other fields.
研究领域:
生物化学
·
分析化学
·
生物技术
·
药理学
·
诊断学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）