BIOSENSORS & BIOELECTRONICS
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2015
·
IF 6.409
Yizhen Yang,Feng Gao,Xili Cai,Xiaoning Yuan,Suyu He,Fei Gao,Hongxu Guo,Qingxiang Wang
A versatile nanocomposite containing β-cyclodextrin and graphene (CD–GR) was prepared through a simple chemical reduction method. The characterization experiments show that the nanocomposite remains the flake-like morphology of GR, but its solubility and stability in aqueous solution are greatly improved. Then the nanocomposite was modified at glassy carbon electrode (GCE) surface, and was used as a functional matrix for the covalent immobilization of probe DNA using 2,4,6-trichloro-1,3,5-triazine (TCT) as the crosslinker . Due to the synergetic effect of large surface area of GR and rich hydroxyl of CD, the probe density for the developed biosensor was determined to as high as 3.82×10 13 molecules cm − 2 . Meanwhile, the biosensor shows high hybridization efficiency and hybridization kinetic. When the biosensor was applied for the impedance-based hybridization test, a wide linear range from 1.0×10 −16 to 1.0×10 −12 M and an ultralow detection limit of 3.4×10 −17 M were achieved. The biosensor also displays excellent stability, selectivity , and reproducibility.
研究领域:
纳米技术
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生物传感器
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 4
Tian Wei,Wang Xin-Yuan,Feng Die-Wen,Li Xiang-Qian,Jin Yue-Kang,Li Hui,Liu Hao
In this study, an amine-reactive poly(pentafluorophenyl acrylate) ( PPFPA ) platform was developed for advanced surface engineering of next-generation sequencing (NGS) chips. Through post-polymerization modification, PPFPA was functionalized with dual moieties: azide groups for covalent immobilization of DBCO-modified DNA primers via click chemistry and tunable hydrophilic side chains to optimize biocompatibility and surface properties. Systematic screening revealed that hydrophobic azide carriers combined with neutral hydroxyl groups maximized the DNA immobilization efficacy, approaching the performance of commercial polyacrylamide-based polymers. The negatively charged carboxyl groups severely impede DNA primer attachment. Higher molecular weight derivatives further enhance the efficacy of DNA immobilization. In NGS validation, optimized surface modification polymers achieved robust surface density of clustered DNA and high sequencing accuracy, surpassing quality benchmarks and comparable to those of conventional analogs. This platform demonstrates significant potential for tailoring high-sensitivity surfaces for genomic applications, advancing clinical diagnostics, and personalized medicine.
研究领域:
高分子科学
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表面工程
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基因组学
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生物化学
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生物技术
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临床诊断
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个性化医疗
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产品关联
DOI
高影响因子
BIOPOLYMERS
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2013
·Vol. 101
·期 5
·496-503
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IF 2.879
Zhihong Zhang,Shunli Liu,Yu Shi,Jun Dou,Shaoming Fang
This study investigates the application of Plasma-polymerized pyrrole (ppPY) as bioactive platform for DNA immobilization and cell adhesion based on the fundamental properties of ppPY, such as chemical structure, electrochemical property, and protein adsorption. Variations in electrochemical properties of the ppPY film deposited under different plasma conditions before and after DNA immobilization were measured using electrochemical impedance spectroscopy (EIS). The equilibrium concentration of the probe DNA immobilized on the ppPY surface was deduced by detecting the variations in the surface charge transfer resistance ( R ct ) of the ppPY films after DNA immobilization with different concentrations. In addition, the detection limit of the target DNA hybridization with probe DNA, the association constant, K a , and the dissociation constant were deduced from Langmuir isotherm equations simulated using the experimental data collected by EIS. Moreover, inverted microscope was used to observe the cell adhesions onto the surface of the ppPY films prepared under different plasma conditions. Different adhesive behaviors of cells were observed, demonstrating that ppPY films could be an alternative biomaterial used as the sensitive layer for DNA sensor or cell adhesion. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 496–503, 2014.
研究领域:
生物材料
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电化学
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分子生物学
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细胞粘附
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DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 137
·期 2
·414-419
·
IF 3.913
Lina Zou,Ying Xu,Peili Luo,Shusheng Zhang,Baoxian Ye
A novel voltammetric sensor, based on DNA immobilized on the surface of an ethylenediamine/polyglutamic (En/PGA) modified glassy carbon electrode (GCE), was constructed and used for determination of dihydromyricetin (DMY). The electrochemical behaviour of DMY at this sensor was investigated in pH 3.6 NaAc-HAc buffer solutions by cyclic voltammetry (CV) and differential pulse anodic voltammetry (DPV). The oxidation of DMY is an adsorption-controlled irreversible process. The oxidation mechanism was proposed and discussed. It was found that the modified electrode exhibited a linear voltammetric response for DMY in the range of 4.0 × 10−8 mol L−1 to 2 × 10−6 mol L−1, with a detection limit of 2 × 10−8 mol L−1. The method was also applied successfully to detect DMY in an ampelopsis sample with satisfactory results.
研究领域:
分析化学
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电化学
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药物分析
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DOI
PubMed
高影响因子
RSC Advances
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2025
·Vol. 15
·期 32
·26362-26370
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IF 4.6
Han Wu,Jiayao Fang,Jiao Chen,Yaoqi Wang,Bo Zheng
Protein display technology enables high-throughput screening and plays an important role in protein discovery and engineering. Conventional in vivo display methods face challenges such as inefficient gene transformation and complex cell proliferation dynamics, while in vitro display methods are often limited to affinity-based selection and suffer from expression bias due to homogeneous reaction conditions. Here, we present a hydrogel particle-based protein display method enabled by particle-templated emulsification. This approach uses functionalized polyacrylamide hydrogel particles as isolated microreactors, incorporating DNA primers for genotype immobilization and Ni-NTA groups for capturing histidine-tagged protein phenotypes. Displayed proteins are synthesized via cell-free protein expression within isolated droplets, overcoming the limitations of in vivo cell culture and enabling compartmentalized screening, which is challenging in conventional homogeneous in vitro systems. Using particle-templated emulsification, single hydrogel particles can be rapidly encapsulated with individual DNA templates into isolated water-in-oil droplets within 30 seconds, without the need for specialized instrumentation. Up to 109 particles can be emulsified in a standard 50 ml conical tube. Compared to conventional droplet microfluidics, particle-templated emulsification achieves higher single-particle encapsulation and improved one-to-one particle–DNA pairing efficiency, reducing reagent consumption and minimizing DNA library loss caused by improper pairing. Digital PCR and cell-free protein expression are sequentially performed within droplets, with both the amplified DNA and the expressed protein immobilized on the same particle, thereby establishing a stable genotype–phenotype linkage. This method eliminates the need for cell handling, enables compartmentalized functional screening, and provides a fast, scalable, and user-friendly workflow for protein display, offering strong potential in directed evolution and protein engineering.
研究领域:
生物化学
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生物技术
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蛋白质工程
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合成生物学
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分子生物学
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微流控技术
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定向进化
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产品关联
DOI
PubMed
高影响因子
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