TALANTA
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2019
·
IF 4.916
Chen Liu,Jialun Han,Jingjing Zhang,Jie Du
A novel detection platform based on Exonuclease III dual-cycle signal amplification with graphene oxide as quenching agent and DNA labelling as recognition element has been developed for the fluorometric determination of insulin. Specific DNA sequences were cut by Exo III to control the fluorescence recovery of the system. Fluorescence intensity analysis was carried out by fluorescence spectrophotometer to obtain the fluorescence intensity corresponding to different concentrations of insulin. When insulin is added to the system, insulin binds to the adaptor and the fluorescence is quenched, whereas in the absence of insulin the fluorescence is restored. The sensor platform achieved multiple signal amplification affording rapid and sensitive detection of insulin with a detection range of 0.048–2.15 U/ml. The methodology is rapid, sensitive, of low cost and convenient to perform and has potential for routine screening of insulin in blood, and also has prospects for the detection of other proteins and biomolecules.
研究领域:
生物化学
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医学检测
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Yi Wu,Guobao Zhou,Zeyu Ma,Dan Zhang,Jianrong Chen,Lei Li
Deep understanding of the effect of DNA sequence and structure on the fluorescent properties of DNA-templated silver nanoclusters (DNA–Ag NCs) affords alternative strategies for developing and exploiting DNA biosensors. DNA secondary structures (e.g., DNA loop) are essential for generating highly emissive DNA–Ag NCs. In this study, a cytosine-rich DNA loop was used as a facile template for the formation of silver nanoclusters. The fluorescence of silver nanocluster encapsulated within a cytosine-rich DNA loop was turned on by switching the size of the loop from large to small. Target nucleic acid served as the trigger for the hybridization chain reaction of hairpin probe 1 (H1) containing an inactivated cytosine-rich DNA loop with a large size at the ends and hairpin probe 2 (H2). Long-nicked double helices were obtained coupled with the opening of the large-size cytosine-rich DNA loop in H1 and switching to an activated cytosine-rich DNA loop with a small size. As a result, an increase in the fluorescent signal was observed. A sensitive detection limit of 10 pM and a linear detection range from 10 pM to 400 nM were obtained for nucleic acid determination. This facile assay provides a new approach capable of switching the fluorescence emission of DNA–Ag NCs for nucleic acid biosensing.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 10
·1149-1153
·
IF 1.618
Li Na,Liu Shi Gang,He Yong Qin,Mai Xi
A resonance Rayleigh scattering (RRS) technique was utilized as a tool for isoelectric point monitoring and iron(III) cation determination. The spectral properties of some amphoteric molecules (proteins and a DNA sequence) were investigated using the RRS technique. When the pH values were kept at around their isoelectric points, especially high RRS signals could be obtained, which were much stronger than those at other pH values. By using the C30 DNA sequence as a probe, the iron(III) cation can be detected rapidly. After iron(III) was added to a C30 solution, a significantly decreased RRS signal was obtained. The sensing process can be finished within 10 min with a detection limit of 0.9 μM. Thus, a sensitive, selective, and label-free method was successfully developed for iron(III) detection.
研究领域:
分析化学
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生物化学
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光谱学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 408
·期 3
·683-693
·
IF 3.436
Chen Xing,Jiang Guimei,Wang Zhili,Hong Shanni,Zhang Yuanyuan,Guo Yahui,Cheng Hui,Wang Jine,Pei Renjun
Doxorubicin (Dox) is a DNA-targeting anthracycline antibiotic active against a wide spectrum of cancers. The interaction between Dox and double-stranded DNA (dsDNA) was used to load Dox using DNA duplexes as carriers. More importantly, the interesting DNA sequence-dependent fluorescence response of Dox could be exploited in the design of efficient Dox release systems and efficient fluorescence sensors. In this work, we demonstrated that separate introduction of G and C bases into T-rich single-stranded DNA (ssDNA) sequences afforded the best discrimination of Dox binding between dsDNA and ssDNA. For the first time, we successfully utilized this interesting DNA sequence-dependent fluorescence response of Dox as a signal transduction mechanism for the sensitive detection of biothiols in human serum. Cysteine, homocysteine, and glutathione were detected at as low as 26 nM, 37 nM, and 29 nM, respectively. The biosensors exhibited not only good selectivity, stability, and sensitivity in aqueous solutions but also a sensitive response in human serum, demonstrating their potential for diagnosis.
研究领域:
生物化学
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医学诊断
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2014
·
IF 4.517
Lichun Zheng,Xiuhui Liu,Min Zhou,Yongjun Ma,Guofan Wu,Xiaoquan Lu
We presented a new strategy for ultrasensitive detection of DNA sequences based on the novel detection probe which was labeled with Ag + using metallothionein (MT) as a bridge. The assay relied on a sandwich-type DNA hybridization in which the DNA targets were first hybridized to the captured oligonucleotide probes immobilized on Fe 3 O 4 @Au composite magnetic nanoparticles (MNPs), and then the Ag + -modified detection probes were used to monitor the presence of the specific DNA targets. After being anchored on the hybrids, Ag + was released down through acidic treatment and sensitively determined by a coupling flow injection–chemiluminescent reaction system (Ag + –Mn 2+ –K 2 S 2 O 8 –H 3 PO 4 –luminol) (FI–CL). The experiment results showed that the CL intensities increased linearly with the concentrations of DNA targets in the range from 10 to 500 pmol L −1 with a detection limit of 3.3 pmol L −1 . The high sensitivity in this work may be ascribed to the high molar ratio of Ag + –MT, the sensitive determination of Ag + by the coupling FI–CL reaction system and the perfect magnetic separation based on Fe 3 O 4 @Au composite MNPs. Moreover, the proposed strategy exhibited excellent selectivity against the mismatched DNA sequences and could be applied to real samples analysis.
研究领域:
生物化学
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
·
2018
·
IF 2.701
Cai-Xia Shi,Qing Liu,Zeng-Ping Chen,Ru-Qin Yu
Exonuclease III assisted target recycling amplification strategy can be used to enhance the sensitivity of mass spectrometry for the detection of short DNA sequences. However, the distribution pattern of DNA fragments produced by exonuclease III assisted target recycling amplification is generally different for samples with different concentrations of the target DNA sequence, which hinders the extraction of both qualitative and quantitative information of the target DNA from mass spectral measurements using traditional univariate or multivariate models. In this contribution, an advanced model was derived based on a reasonable assumption for the qualitative and quantitative analysis of the mass spectral measurements of DNA fragments produced by exonuclease III-assisted target recycling amplification. Experimental results demonstrated that the integration of exonuclease III assisted target recycling amplification, mass spectrometry and the advanced model could achieve sensitive and accurate quantitative results for a target short DNA sequence in complex biological medium with a detection limit of 50 pM and a mean recovery rate within the range of 89.5%–106.7%. More interestingly, the proposed model could unambiguously identify single nucleotide polymorphisms based on the distribution patterns of residual DNA fragments. Therefore, with the aid of the proposed model, mass spectrometry based on exonuclease III assisted recycling amplification has great potential for the reliable, sensitive, selective, and relatively low-cost detection and quantification of short DNA sequences in clinical diagnosis and biomedical research.
研究领域:
分子生物学
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临床诊断
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生物医学研究
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DOI
高影响因子
LUMINESCENCE
·
2012
·Vol. 28
·期 6
·860-864
·
IF 1.731
Tingting Chen,Leixiaomeng Fu,Lily Zu
Time-resolved fluorescence as well as steady-state absorption and fluorescence were detected in order to study the interactions between tetramethylrhodamine (TAMRA) and DNA when TAMRA was covalently labeled on single- and double-stranded oligonucleotides. Fluorescence intensity quenching and lifetime changes were characterized and correlated with different DNA sequences. The results demonstrated that the photoinduced electron transfer interaction between guanosine residues and TAMRA introduced a short lifetime fluorescence component when guanosine residues were at the TAMRA-attached terminal of the DNA sequences. The discrepancy of two-state and three-state models in previous studies was due to the DNA sequence selection and sensitivity of techniques used to detect the short lifetime component. The results will help the design of fluorescence-based experiments related to a dye labeled probe. Copyright © 2012 John Wiley & Sons, Ltd.
研究领域:
化学
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分子生物学
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生物物理
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2014
·
IF 2.129
Xiangying Sun,Bin Liu,Chuanxiao Yang,Congcong Li
In this work we designed a fluorescent self-assemblied multilayers, with thrombin aptamers and ssDNA as aptamer fixed onto the outermost layer, respectively. This multilayers can effectively sense biomolecules by interfacial florescence resonance energy transfer from multilayers to graphene oxide. High fluorescence quenching efficiency of graphene oxide and self-assemblied membrane’ concentration results in good sensitivity for biosensing. A new interfacial sensing method with extremely high sensitivity for thrombin and DNA sequence was established, and the detection limit for thrombin and DNA was 16.2 pM and 72.6 fM, respectively.
研究领域:
生物传感器
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分子生物学
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DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 26
·5234-5241
·
IF 1.915
Liu Yang,Yanfu Li
A new electrochemical biosensor based on the hybridization chain reaction and layered molybdenum selenide (MoSe2) stacked nanorod for dual-signal amplification is developed for the highly sensitive detection of the DNA sequences of Human Immunodeficiency Virus type 1 (HIV-1). The MoSe2 nanorod was prepared using a facile hydrothermal method. The glassy carbon electrode was first modified with MoSe2 nanorods and then Au nanoparticles (AuNPs) were electrodeposited on the electrode. The DNA probe was then immobilized on the electrode by an Au–S bond. As the initiator strands, auxiliary DNA can propagate a chain reaction of hybridization events between the two hairpin probes (Aux 1, Aux 2), and long nicked DNA polymers can be formed on the modified electrode. The biotin-labeled dsDNA polymers can then introduce numerous avidin-labeled horseradish peroxidases on the electrode, resulting in a significantly amplified electrochemical signal through the electrocatalysis of horseradish peroxidase. The target DNA is detected on the basis of the enzymatic oxidization of Fe2+ by H2O2. The electrochemical signals have a good linear relationship with the logarithm of target DNA concentration in the range from 10 fM to 0.1 pM, reaching a detection limit of the target DNA as low as 4.0 fM. This method shows good specificity for target DNA detection with the capability to discriminate single-base-pair mismatched DNA mutations. Moreover, the developed strategy was applied successfully in a real sample, which demonstrated the potential applications in clinical diagnostics.
研究领域:
生物传感技术
·
分子诊断学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Huili Shao,Han Lin,Zhiyong Guo,Jing Lu,Yaru Jia,Meng Ye,Fengmei Su,Lingmei Niu,Weijun Kang,Sui Wang,Yufang Hu,Youju Huang
MicroRNAs are widely used as tumor markers for cancer diagnosis and prognosis. Herein, a multiple signal amplification sandwich-type SERS biosensor for femtomolar detection of miRNA is reported. The signal unit consisted of giant Au vesicles, DNA sequences and deposited silver nanoparticles . The giant Au vesicles provided large-volume hot spots because of sharp tips and abundant hotspot gaps, thus enhancing the electromagnetic intensity for the SERS performance. Further silver stain would easily lead to second-stage amplification of Raman signal. In addition, more SERS signal molecules R6G adsorbed on the signal unit with the aid of HCR and the controlled nanogaps between adjacent AgNPs, brought about the third-stage amplification. The capture unit, prepared by immobilizing the capture probe (CP) on the Fe 3 O 4 @AuNPs, could easily capture target miRNA and greatly simplify the separation step to improve reproducibility. The higher concentration of target miRNA definitely formed more sandwich-type structures with combination of capture unit and signal unit, resulting in multiple amplification of SERS signals. The proposed multiple signal amplification sandwich-type SERS biosensor could detect miRNA-141 at the femtomolar level with a low detection limit of 0.03 fM. Meanwhile, it exhibited high selectivity and accuracy, even for practical analysis in human serum. Therefore, the designed multiple signal amplification sandwich-type SERS biosensor would be a very promising alternative tool for the detection of miRNA and analogs in the field of biomedical diagnosis.
研究领域:
生物医学诊断
·
分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2014
·
IF 2.871
Chen Zhang,Shichao Xu,Xueping Zhang,Dandan Huang,Runlan Li,Shihuai Zhao,Bing Wang
In this article, we developed an electrochemical method for detection of specific DNA sequences of bladder cancer cells based on CdTe quantum dots (QDs) modified glassy carbon electrode (GCE). Methylene blue (MB) was intercalated into the hybridized double stranded DNA (dsDNA) and used as electrochemical indictor. CdTe QDs amplified the electrochemical signals due to their larger surface area, which can immobilize more single stranded DNA (ssDNA) probes on electrode surface. The morphology and optical properties of CdTe QDs were characterized by transmission electron microscope (TEM), X-ray powder diffraction (XRD), field emission scanning electron microscopy (FESEM), fluorescence spectra (FS), UV–vis spectrum and Fourier transform infrared spectrum (FTIR). Moreover, target DNA was detected by differential pulse voltammetry (DPV) quantitatively. The linear range was obtained from 1 × 10 −12 M to 1 × 10 −8 M, and the limit of detection was 6.435 × 10 −13 M (3δ). The control experiments were also carried out by hybridization with various mismatched DNA sequences. These results displayed this fabricated electrochemical DNA biosensor was of good selectivity, sensitivity, stability and easy operation.
研究领域:
生物化学
·
电化学
·
癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 1
·73-79
·
IF 1.474
Chomba Haji,Pan Ting,Zhuo Xinyue,Zhao Lan,Wang Yulin,Huang Zhao,Martin Haikael,Chen Dongchu,Nie Libo
Simple, cost effective, high sensitive and selective detection strategies for disease related DNA sequences in clinical diagnostics and research purposes are still on demand. Detection of DNA specific sequences of particular biomedical importance, based on electrochemical signaling,
has been reported as a promising analytical approach for medical diagnostics due to its simplicity, cost effective, sensitivity, selectivity and rapidity. Herein, a simple and cost effective DNA biosensor based on poly T30 Copper Nanoparticle Signaling and biobarcode technique is presented
for the first time. In this design, complementary sequence places the poly T30 modified bio-barcode probe (P2-SiO2-T30) on the sensor interface. Upon copper reduction reaction, copper nanoparticles (CuNPs) are clustered along the poly T30 modified bio-barcode probe (P2-SiO2-T30-CuNPs).
During electrochemical measurements copper nanoparticles (CuNPs) are oxidized to give current signal. This detection strategy has a detection limit of 10 pM.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
医学诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）