SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Jianhui Liu,Deyu Kong,Zhen Liu,Hanbiao Liu,Jiangle Yi,Dong Tian,Fangquan Xia,Changli Zhou
In this paper, a novel amino-functionalized mesoporous dendritic fibrous nanosilica (NH 2 -DFNS) was used as DNA amplification platforms with DNA generated electrochemical signal as target conversion strategy to establish an electrochemical aptasensor for chlorpyrifos residues. The signal generation was based on the electrochemical reaction between molybdate with phosphate groups on the aptamer molecules, which greatly simplified the signal transduction and preparation steps of aptasensor, so as to improve the sensitivity of the aptasensor. The NH 2 -DFNS was prepared by a simple microemulsion method and characterized by SEM, TEM, FT-IR, BET, as well as XPS techniques. Importantly, the results showed that such a sphere-shaped nanomaterial with three-dimensional (3D) centerradial channels and hierarchical pores supported abundant DNA to amplify the DNA generated signal in the aptasensor. Furthermore, this aptasensor for detection of chlorpyrifos showed good sensitivity with a linear range of 1.0 fM∼1.0 nM, a detection limit of 0.43 fM. In addition, this sensor also possessed excellent specificity, repeatability, and stability. Moreover, this proposed electrochemical aptasensor was successfully used for the detection of chlorpyrifos residues in several practical samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 11
·3649-3658
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IF 4.019
Qianqian Fang,Yingying Li,Xinxing Miao,Yiqiu Zhang,Jun Yan,Tainrong Yu,Jian Liu
Serious healthcare concerns have been raised on the issue of antibiotic residues after overuse, especially by accumulation in the human body through food webs. Here, we report a methodological development for sensitive detection of antibiotics with aptamer conformation cooperated enzyme-assisted SERS (ACCESS) technology. We design and integrate a set of nucleic acid oligos, realizing specific recognition of chloramphenicol (CAP) and efficient exonuclease III-assisted DNA amplification. It features a “signal-on” analysis of CAP with the limit of detection (15 fM), the lowest concentration detectable in the literature. Our method exhibits a high selectivity on the target analyte, free of interference of other potential antibiotic contaminants. The ACCESS assay promises an ultrasensitive and specific detection tool for trace amounts of antibiotic residues in samples of our daily life.
研究领域:
分析化学
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生物技术
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食品安全
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环境科学
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DOI
PubMed
高影响因子
LAB ON A CHIP
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2019
·Vol. 19
·期 16
·2663-2668
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IF 6.914
Zhenqing Li,Ruixue Ju,Shinichi Sekine,Dawei Zhang,Songlin Zhuang,Yoshinori Yamaguchi
Current continuous flow polymerase chain reaction (CF-PCR) microfluidic chips require external precision syringe pumps and off-line methods (e.g., electrophoresis and hybridization) to detect PCR products, resulting in complex operations and possible cross-contamination and consequently CF-PCR is still confined to laboratories. Herein, a portable all-in-one microfluidic device is fabricated for rapid diagnosis of pathogens based on an integrated CF-PCR and electrophoresis biochip. A new method was proposed for automatic sample injection into the chip which can substitute the costly external precision syringe pump. It not only achieves rapid DNA amplification and on-site PCR product detection, but also realizes automatic sample injection. As an application, three periodontal pathogens (e.g., Porphyromonas gingivalis, Treponema denticola and Tannerela forsythia) were successfully amplified in the device. Treponema denticola was amplified in as short as 2′31′′, and detection of PCR products was completed within 3′43′′. The minimum number of bacteria that can be amplified was 125 cfu per μl. The all-in-one device has the potential to be applied in point-of-care nucleic acid testing for diseases.
研究领域:
微流控技术
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生物医学工程
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分子诊断
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即时检测
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
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2018
·Vol. 6
·期 36
·9703-9712
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IF 5.976
Qi You,Yang Chen
Gold nanoclusters (AuNCs) as a new class of luminescent materials have attracted a great deal of interest owing to their molecule-like properties and wide potential applications. However, limited successes have been achieved in producing intensely luminescent AuNCs. This paper reports a simple and convenient preparation method of strongly luminescent, highly heat-stable AuNCs by using functional ligands and a hydrothermal reaction. Adenosine cyclic monophosphate (cAMP) and tryptophan (Trp) are used as a template molecule and energy antenna ligand of AuNCs, respectively. By means of a common hydrothermal reaction, the resulting tryptophan-stabilized AuNCs (Trp–cAMP–AuNCs) generated surprising luminescence with a quantum yield of up to 59.6%; their luminescence is highly heat-stable, and they can be applied as a luminescent label in PCR (polymerase chain reaction) thermal cycles of DNA amplification. This unusually strong luminescence is attributed to the double effects of energy transfer from the ligand and hydrothermally-induced emission enhancement. The indole groups strongly interacting with gold atoms play an important role for the thermal-stability of the luminescence. The as-prepared AuNCs are also highly water-soluble and storage-stable. The present strategy offers a new way for and insight into the fabrication of strongly luminescent metal nanoclusters.
研究领域:
纳米技术
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材料科学
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化学
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生物技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Jingzhi Zhou,Xichen Shi,Chaoqin He,Xiuwen Zheng,Ruo Yuan,Xia Yang
Background Entropy-Driven Circuits (EDC), distinguished by their spontaneous operation and absence of enzymatic reactions, represent a superior strategy for integration with CRISPR/Cas systems, as they obviate the potential for interference among various enzymes during the process of DNA amplification and CRISPR/Cas system integration. Due to the wide band gap of TiO 2 , its response to visible light is limited, and owing to its high crystallinity and exceptionally stable crystal lattice, the charge transfer (CT) process in TiO 2 is suboptimal. Results In this study, lychee-like Fe–TiO 2 was firstly prepared to serve as Raman enhanced substrate, facilitating exciton capture and separation to exhibit an excellent Surface-enhanced Raman spectroscopy (SERS) performance. It is proven that the incorporation of Fe results in a significantly narrower band gap for TiO 2 , facilitating exciton resonance. The amount of Fe in TiO 2 was optimized to fabricate a SERS biosensor for detection of miRNA-21 based on the Cas13a assisted entropy-driven system. The detection limit of miRNA was 43.88 fmol/L. Significance This work proposes a sensing strategy that integrates the CRISPR/Cas system with EDC, leveraging a semiconductor substrate exhibiting superior SERS performance to provide a stable Raman signal, thereby enabling highly sensitive detection of miRNA-21, which has a potential application in disease early warning and treatment.
研究领域:
生物传感器
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纳米技术
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生物化学
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材料科学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Liang-Liang Wang,Wen-Qian Chen,Yu-Ru Wang,Lu-Peng Zeng,Ting-Ting Chen,Guan-Yu Chen,Jing-Hua Chen
Exosomal microRNAs (miRNAs) have been explored as an extremely promising biomarker of liquid biopsy for the diagnosis, treatment and prognosis of diseases such as cancer, in which sensitive and selective detection is significant. Herein, we describe the construction and testing of an electrochemical biosensor for the sensitive detection of exosomal miRNAs. It is based on synthetizing numerous long single-stranded DNAs (ssDNAs), which are produced by dual amplification reactions of target-triggered cyclic strand displacement reaction (TCSDR) and primer exchange DNA amplification reaction (PEDAR). In the first signal amplification step, target miRNAs are captured by the hairpin DNA strands (capture probes, Cp) that are immobilized on electrode. After strand unfolding with target capture, primer probes (Pp) enable to hybridize with Cp. And then target miRNAs were displaced for starting the TCSDR process that enable the introduction of numerous primers in Pp. In the second signal amplification step, the primers associated with PEDAR produce copious amounts of elongated ssDNAs. These ssDNAs absorb abundant quantities of methylene blue (MB) that enables the highly sensitive and label-free detection of exosomal miRNAs. This dual amplification process is characterized by a low limit of detection (LOD) of 3.04 aM. In addition, the electrochemical biosensor exhibits good selectivity for miR-21 detection, and shows benefits of simple operation, low cost, portability. Overall, the electrochemical biosensor provides a promising platform for the early diagnosis and screening of tumor biomarkers and the development of devices for point-of-care testing (POCT).
研究领域:
生物标志物检测
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电化学
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癌症诊断
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即时检测
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Guotai Yang,Xiangyu Meng,Yutong Wang,Mingyao Yan,Zoraida P. Aguilar,Hengyi Xu
A novel 2-step lectin-magnetic separation (LMS) method combined with gold nanoparticle (AuNPs) -based colorimetric system for specifically and quantitatively detecting S. aureus in blood samples was established. In this study, wheat germ agglutinin lectin from Triticum vulgaris (wheat) (WGA) was applied for the first time as a recognition agent in a 2-step magnetic separation method using magnetic nanoparticles (MNPs) for effective and low-cost bacteria enrichment. A quantitative detection strategy based on the long dsDNA amplicons stabilized with AuNPs against salt-induced aggregation was investigated. The 2-step LMS method resulted in a high enrichment efficiency even at low concentrations (10 0 –10 2 CFU/mL) of S. aureus in pure culture and blood samples. The AuNPs-based colorimetric system was applied for quantitative detection of S. aureus with a limit of detection (LOD) at 3.5 × 10 0 CFU/mL. The complete process starting with the blood samples treatment, 2-step LMS, and AuNPs-based colorimetric detection took only 7 h. The low cost and sensitive 2-step LMS combined with AuNPs-based colorimetric detection holds considerable potential for early sepsis diagnosis.
研究领域:
生物技术
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纳米技术
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微生物学
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临床诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）