CHEMISTRY-A EUROPEAN JOURNAL
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2023
·Vol. 29
·期 57
·e202301968
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IF 4.3
Zhuo Zhang,Ting Liu,Yawen Li,Yang Yu,Lin Ning,Xuefan Qiu,Chencan Li,Ling Wang,Wenlong Xu
Graphical We demonstrate the temporal programming of the assembly and disassembly of EuW 10 nanospheres using a pH clock reaction of acid/urease hydrolysis. The pH-responsive C 12 DMAO endow EuW 10 nanospheres with dynamical properties, in which fluorescence changes are coded in this system. These fluorescent nanospheres provide new application in time-programmed information encryption. Self-assembly exploits noncovalent interaction to offer an effective method for the fabrication of materials. For Na 9 [EuW 10 O 36 ] ⋅ 32H 2 O (EuW 10 ), the negative charges and abundant oxygen atoms on its surface provide a handle for static self-assembly. New properties are envisioned for EuW 10 aggregates which are able to display such kinetics and time-programming characteristics, in order to satisfy more complex and intelligent application scenarios, such as DNA binding and information encryption. In this work, EuW 10 coupling with stimuli-responsive dodecyl dimethylamine oxide (C 12 DMAO) can generate versatile aggregates with pH-responsive properties. We demonstrated the temporal programming of the assembly and disassembly of EuW 10 nanospheres using a pH clock reaction of acid/urease hydrolysis. The pH clock reaction endows EuW 10 assemblies with dynamical properties, in which the charges and fluorescence changes are coded in this system. These fluorescent assemblies provide new application in time-programmed DNA capture and information encryption.
研究领域:
材料科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
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2015
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IF 4.772
Di Bao,Peng Gao,Yanbo Wang,Han Zhou,Yujin Chen,Guorui Chen,Piaoping Yang,Xitian Zhang
In this paper, Cd(OH)2 1D three-side nanobelt architectures have been fabricated through a facile two-phase reaction system. Considering their electropositive character proven by zeta potential measurement, the as-obtained Cd(OH)2 nanomaterials have been applied to capture negatively charged DNA. On the basis of the above results, the Cd(OH)2 nanomaterials have been employed for trapping negatively charged double-stranded DNA molecules (salmon sperm DNA). Assisted by the detailed observation of their DNA interaction results as well as through UV–vis spectroscopy, infrared imaging automatic target recognition (IRATR) and inductive coupled plasma atomic emission spectrometer (ICP-AES) measurements, it has been clearly shown that this 1D nanoarchitecture can effectively trap and release DNA molecules without use of any other organic reagents. In addition, a miniaturized electrochemical DNA-releasing device based on the Cd(OH)2 1D three-side nanobelt architectures has been fabricated. Using this device, it is possible to recycle the Cd(OH)2 nanostructures. In addition, the Cd(OH)2 nanomaterials maintain their nanostructures, and no Cd2+ ions were released in repeated DNA separation and transfer processes, which demonstrate their practicality for DNA trapping.
研究领域:
纳米材料科学
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生物物理学
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分子生物学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Yalong Bai,Yan Cui,George C. Paoli,Chunlei Shi,Dapeng Wang,Min Zhou,Lida Zhang,Xianming Shi
Magnetic separation has great advantages over traditional bio-separation methods and has become popular in the development of methods for the detection of bacterial pathogens, viruses, and transgenic crops. Functionalization of magnetic nanoparticles is a key factor for efficient capture of the target analytes. In this paper, we report the synthesis of amino-rich silica-coated magnetic nanoparticles using a one-pot method. This type of magnetic nanoparticle has a rough surface and a higher density of amino groups than the nanoparticles prepared by a post-modification method. Furthermore, the results of hydrochloric acid treatment indicated that the magnetic nanoparticles were stably coated. The developed amino-rich silica-coated magnetic nanoparticles were used to directly adsorb DNA. After magnetic separation and blocking, the magnetic nanoparticles and DNA complexes were used directly for the polymerase chain reaction (PCR), without onerous and time-consuming purification and elution steps. The results of real-time quantitative PCR showed that the nanoparticles with higher amino group density resulted in improved DNA capture efficiency. The results suggest that amino-rich silica-coated magnetic nanoparticles are of great potential for efficient bio-separation of DNA prior to detection by PCR.
研究领域:
纳米技术
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Cell Death & Disease
·
2024
·Vol. 15
·期 12
·1-14
·
IF 8.1
Chen Li,Zou Yahui,Sun Renhong,Huang Mei,Zhu Xiaotong,Tang Xiao,Yang Xiaobao,Li Dake,Fan Gaofeng,Wang Yu
Poly (ADP-ribose) polymerase 1 (PARP1) catalyzes poly (ADP) ribosylation reaction, one of the essential post-translational modifications of proteins in eukaryotic cells. Given that PARP1 inhibition can lead to synthetic lethality in cells with compromised homologous recombination, this enzyme has been identified as a potent target for anti-cancer therapeutics. However, the clinical application of existing PARP1 inhibitors is restrained by side effects associated with DNA trapping and off-target effects, highlighting the need for improved therapeutic strategies. By integrating protein degradation technology, we synthesized a PROTAC molecule 180055 based on the Rucaparib junction and VHL ligand, which efficiently and selectively degraded PARP1 and inhibited PARP1 enzyme activity without a noticeable DNA trapping effect. Furthermore, 180055 kills tumor cells carrying BRCA mutations with a minor impact on the growth of normal cells both in vitro and in vivo. This suggests that 180055 is a PARP1-degrading compound with excellent pharmacological efficacy and extremely high biological safety that deserves further exploration and validation in clinical trials.
研究领域:
肿瘤学
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分子生物学
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药理学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Jiayao Yang,Jie Fei,Xiaohuan Liu,Hongpeng Wang,Jun Huang
The advancement of biomedicine and molecular diagnostics depends on effective nucleic acid separation methods. Nevertheless, creating high-performing and environmentally friendly nucleic acid adsorbent materials is still challenging. In order to achieve high-quality DNA capture, this work creatively created a rare earth metal hybridized magnetic chitosan nanoparticle (Fe 3 O₄@CS@La(OH)₃) by combining the biocompatibility of chitosan with the effective coordination ability of La 3+ . The results showed that Fe₃O₄@CS@La(OH)₃ had a high specific surface area and superparamagnetism, with a high adsorption capacity of DNA up to 370.37 mg/g, and 80 % of DNA could be captured within 5 min, and the capture rate was up to 96.7 % within 2 h. This was primarily ascribed to the strong coordination and electrostatic interaction between La 3+ and nucleic acids. Furthermore, the adsorption efficiency continued to exceed 90 % after five cycles, indicating exceptional reproducibility. This study involved the novel combination of the rare earth metal lanthanum with magnetic chitosan. The utilization of its unique coordination chemistry significantly enhanced the capture efficiency of nucleic acids, presenting potential applications in precision medicine and disease detection.
研究领域:
生物医学工程
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纳米技术
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分子诊断学
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Shiyu Li,Kui He,Rong Liao,Chunyan Chen,Xiaoming Chen,Changqun Cai
An interference-free and label-free sensing platform was developed for the highly sensitive detection of microRNA-21 (miRNA-21) in vitro by magnetic silicon microsphere (MNP)-reduced graphene oxide (rGO)-based sandwich probe. In this method, DNA capture probes (P 1 ) were connected with MNPs at the 5′ end and hybridized with completely complementary target miRNA. Subsequently, rGO was retained and induced the fluorescence quenching in the supernatant. Through the magnetic separation, the supernatant environment was simplified and the interference to analytical signal was eliminated. When DNA capture probe-modified magnetic silicon microspheres (MNP-P 1 ) were adsorbed through rGO in the absence of a target and formed a sandwich structure, the formed nanostructure was easily removed from the solution by a magnetic field and the fluorescence intensity was maximally recovered. This proposed strategy, which both overcame the expensive and cumbersome fluorescent labeling , and eliminated interference to analytical signal for guaranteeing high signal-to-background ratio, exhibited high sensitivity with a detection limit as low as 0.098 nM and special selectivity toward miRNA-21. The method was potentially applicable for not only detection of miRNA-21 but also various biomarker analyses just by changing capture probes.
研究领域:
生物传感器
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纳米技术
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分子诊断
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
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