BIOCHEMICAL ENGINEERING JOURNAL
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2016
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IF 2.463
Xiangmin Miao,Zhiyuan Cheng,Zongbing Li,Po Wang
This work described a label-free platform for sensitive cholesterol detection upon the precipitation of cetyltrimethyl ammonium bromide (CTAB) stabilized gold nanoparticles (AuNPs). Modifying with CTAB, the stability of AuNPs was greatly improved compared to conventional used AuNPs, and the surface of them was positively charged, which could effectively adsorb with negatively charged DNA detection probe (DP) based on electrostatic adsorption, and inducing the precipitation of them. Tremendous H 2 O 2 would produce based on the reaction of cholesterol with oxygen by using cholesterol oxidase (ChO x ) as the catalyzer, and followed the happen of Fenton reaction in the presence of Fe 2+ to generate OH , inducing the cleavage of DP into DNA fragments. As a result, the adsorption ability of AuNPs decreased, accompanying with a decrease of AuNPs precipitation. Then, based on detecting the absorption spectra change of AuNPs in supernatant along with the increase of cholesterol concentration, sensitive and simple visual-based cholesterol detection was realized with a detection limit of 335 pM. Such method showed high selectivity for the detection of cholesterol. Moreover, the high stability of such AuNPs in solution that contained salt, protein or other metal ions, making our sensing method have a potential application in bioanalysis.
研究领域:
生物化学
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医学检测
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纳米技术
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DOI
高影响因子
Small Methods
·
2021
·Vol. 5
·期 12
·2101072
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IF 14.188
Xiyue Xie,Zhenqiang Wang,Meizhen Zhou,Yuxin Xing,Yuhua Chen,Jixi Huang,Kaiyong Cai,Jixi Zhang
Electrochemical nanosensors by integrating functional nucleic acids and nanomaterials hold a great promise in the fast detection of biomarkers, yet the current systems possess limitations on the accessibility of target–probe and probe–electrode interactions and the repeatability of detection. Herein, a host–guest assembly strategy is developed to build redox nanosensors for an immobilization-free and ratiometric electrochemical detection system. Specifically, electroactive molecule ( E m ) guests are loaded in porous hosts of polydopamine nanoparticles (MPDA) to act as dual-signal redox reporters. Hybrid DNA probes of G-quadruplex and a single-stranded anchor DNA are installed as gatekeepers for sealing the mesopores. Thereby, miRNA triggered E m release by strand displacement reactions and the homogeneous transportation of the hosts/guests to the electrode facilitate the generation of reference signal/response signal at different potentials. Concomitantly applied NIR irradiation boosts the electron transfer from MPDA to the electrode and results in a tenfold increase in the reference signal. Finally, the sensing system through the differential pulse voltammetry method achieves a highly repeatable detection (relative standard deviation 3.8%) of miRNA with a lower detection limit (362 × 10 −15 m ). This attractive system paves the way for rational designs of advanced electrochemical biosensors and smart diagnosis.
研究领域:
生物医学工程
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2016
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IF 4.035
Weijun Zhou,Jiangtao Ren,Jinbo Zhu,Zhixue Zhou,Shaojun Dong
Herein we report a colorimetric biosensing strategy to discriminate single-nucleotide mutation in DNA with high selectivity using unmodified gold nanoparticles (AuNPs) as indicators. In the AuNPs-based colorimetric strategy, binary DNA probes were produced by splitting a long DNA probe in the middle for sensitive differentiation of single-base mismatch. The detection limit of this method toward target DNA was 5 nM. The developed system has superior advantages of utilization of inexpensive materials, simplicity and visualization. Moreover, binary DNA probes not only can distinguish single-base mutation in the target DNA very well, as compared to long DNA probe, but also can construct “AND” logic gate using two distinct target DNAs as inputs, which holds great potential for increasing the accuracy of disease diagnosis in clinical applications.
研究领域:
生物化学
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分子诊断
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纳米技术
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DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Chen Yonghuan,Li Xiuying,Yue Xinru,Yu Weihua,Shi Yuesen,He Zilong,Wang Yuanfeng,Huang Yu,Xia Fan,Li Fengyu
Synthetic drugs fundamentally reshape the illicit drug market due to their low cost, ease of production, and rapid manufacturing processes. However, current drug detection methods, which rely on complex instruments, have limited applicability and often neglect the influence of pH fluctuations, leading to potential bias and unreliable results. Herein, we propose co-calibration DNA probes on a nanoconfined biosensor (N C B S ), covering the range of sweat pH 3–8 to achieve significantly enhanced target signal recognition. The N C B S exhibits a linear response range of 10 3 -10 8 fM with a low limit of detection (LOD) of 3.58 fM in artificial sweat. Compared to the single-aptamer N C B S , the dual-aptamer N C B S offers a broader linear response range, primarily due to the synergistic effects of changes in surface wettability and the capture of hydrion, which together reduce signal interference in proton transport. The linear response range doubles, and its detection sensitivity improves by 4–5 orders of magnitude compared to existing drug detection methods. This sensing strategy expands the application scope of aptamer-based composite probes, offering an approach for ultra-sensitive drug detection and demonstrating significant potential in sweat sensing and drug monitoring fields.
研究领域:
生物传感
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分析化学
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药物检测
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
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IF 8.097
Qian Zhao,Jiafang Piao,Weipan Peng,Yang Wang,Bo Zhang,Xiaoqun Gong,Jin Chang
Identifying the microRNA (miRNA) expression level can provide critical information for early diagnosis of cancers or monitoring the cancer therapeutic efficacy. This paper focused on a kind of gold-nanoparticle-coated polystyrene microbeads (PS@Au microspheres)-based DNA probe as miRNA capture and duplex-specific nuclease (DSN) signal amplification platform based on an RGB value readout for detection of miRNAs. In virtue of the outstanding selectivity and simple experimental operation, 5′-fluorochrome-labeled molecular beacons (MBs) were immobilized on PS@Au microspheres via their 3′-thiol, in the wake of the fluorescence quenching by nanoparticle surface energy transfer (NSET). Target miRNAs were captured by the PS@Au microspheres-based DNA probe through DNA/RNA hybridization. DSN enzyme subsequently selectively cleaved the DNA to recycle the target miRNA and release of fluorophores, thereby triggering the signal amplification with more free fluorophores. The RGB value measurement enabled a detection limit of 50 fM, almost 4 orders of magnitude lower than PS@Au microspheres-based DNA probe detection without DSN. Meanwhile, by different encoding of dyes, miRNA-21 and miRNA-10b were simultaneously detected in the same sample. Considering the ability for quantitation, high sensitivity, and convenient merits, the PS@Au microspheres-based DNA probe and DSN signal amplification platform supplied valuable information for early diagnosis of cancers.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 25
·3081-3088
·
IF 2.073
Xiao-Feng Wang,Yong-Sheng Wang,Xi-Lin Xiao,Wen-Bo Lan,Bin Zhou,Si-Han Chen,Jin-Hua Xue
We report a label-free ultrasensitive and selective strategy for Pb(II) assay by a multifunctional DNA probe (MDP)-mediated rolling-circle amplified synthesis of the G-quadruplexes. The MDP acted as a target recognition probe, a catalytic DNAzyme and a primer of rolling-circle amplification (RCA). The presence of Pb(II) can induce the conformational switching of the MDP, resulting in the cleavage of the S-DNA in the MDP to release an E-DNA. The released E-DNA then initiated a RCA reaction with a reasonably devised padlock DNA template to produce an accumulated amount of repeated sequences. The RCA product in the present work was designed as a G-rich sequence, which could fold into thousands of G-quadruplex units. The G-quadruplex formed by RCA can specifically bind to NMM to result in an amplified fluorescence signal. Through these cascade amplifications, Pb(II) ions can be detected at as low as 94.29 pM, which is much lower than those reported in related literature. We expect that this amplification strategy might be helpful in the design of a highly sensitive analytical platform for wide application in environmental and biomedical fields.
研究领域:
生物化学
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分析化学
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环境科学
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DOI
高影响因子
RSC Advances
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2022
·Vol. 12
·期 21
·13448-13455
·
IF 4.036
Wancun Zhang,Pin Zhang,Ying Liang,Weyland Cheng,Lifeng Li,Huanmin Wang,Zhidan Yu,Yan Liu,Xianwei Zhang
Rapid, simple, sensitive and specific approaches for mercury(II) (Hg2+) detection are essential for toxicology assessment, environmental protection, food analysis and human health. In this study, a ratiometric hairpin DNA probe based electrochemical biosensor, which relies on hairpin DNA probes conjugated with water-soluble and carboxyl functionalized quaternary Zn–Ag–In–S quantum dot (QD) on screen-printed gold electrodes (SPGE), referred to as the HP-QDs-SPGE electrochemical biosensor in this study, was developed for Hg2+ detection. Based on the “turn-off” reaction of a hairpin DNA probe binding with a mismatched target and Hg2+ through the formation of T–Hg2+–T coordination, the HP-QDs-SPGE electrochemical biosensor can rapidly quantify trace Hg2+ with high ultrasensitivity, specificity, repeatability and reproducibility. The conformational change of the hairpin DNA probe caused a significant decrease in electrochemical intensity, which could be used for the quantification of Hg2+. The linear dynamic range and high sensitivity of the HP-QDs-SPGE electrochemical biosensor for the detection of Hg2+ was studied in vitro, with a broad linear dynamic range of 10 pM to 1 μM and detection limits of 0.11 pM. In particular, this HP-QDs-SPGE electrochemical biosensor showed excellent selectivity toward Hg2+ ions in the presence of other metal ions. More importantly, this biosensor has been successfully used to detect Hg2+ in deionized water, tap water, groundwater and urine samples with good recovery rate and small relative standard deviations. In summary, the developed HP-QDs-SPGE electrochemical biosensor exhibited promising potential for further applications in on-site analysis.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-7
·
IF 5.479
Xu Chengnan,Ying Yibin,Ping Jianfeng
The authors describe a colorimetric method for determination of kanamycin by using gold nanoparticles (AuNPs) as the element of signal-conversion and by applying hybridization chain reaction-assisted signal amplification. The assay is carried out by monitoring the absorbance change and color change adding salt to the reaction solution containing kanamycin (analyte), hairpin DNA probe, and AuNPs. Three hairpin DNA probes with sticky ends were absorbed on the AuNPs via their sticky ends. Cating with DNA prevents them from salt-induced aggregation (which leads to a color change from red to blue) in the complete absence of kanamycin. In contrast, in the presence of kanamycin, the aptamer hairpin DNA probe binds kanamycin, and the newly exposed section of DNA triggers a cascade of hybridization chain reactions with formation of numerous dsDNAs. On addition of salt, the AuNPs form blue aggregates due to the repulsion between dsDNA and AuNPs. Under optimal conditions, the ration of absorbance at 520 and 630 nm drops with the kanamycin concentration in the range from 1 to 40 μM, and the limit of detection is 0.68 μM. The assay can selectively distinguish kanamycin from other antibiotics. The method was applied to kanamycin detection in (spiked) milk samples and gave excellent recoveries.
Schematic presentation of colorimetric method for kanamycin detection using gold nanoparticles modified with hairpin DNA probes and hybridization chain reaction-assisted amplification.
研究领域:
分析化学
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Zhang Kai,Cao Jinxuan,Wu Yongxiang,Hu Futao,Li Tianhua,Wang Ying,Gan Ning
A simple and highly sensitive fluorometric method is described for the determination of the antibiotic kanamycin (Kana) in food. Dual signal amplification is accomplished by making use of double Y-shaped aptamer DNA probes acting as a capture probes and signal amplification probes. The DNA probes were immobilized on a gold bar and on a magnetic bar, respectively. On addition of Kana, the Y-shaped aptamer probe captures Kana and then is disassembled to release two single-stranded DNAs. These trigger target recycling and HCR between the two bars simultaneously. As a result, many long duplex DNA chains are formed in the supernatant. After pulling out the bars and adding the fluorescent intercalating probe SYBR Green I, strong fluorescence (with excitation/emission peaks at 497/525 nm) is induced. The use of such double Y-shaped DNA probes obviously overcomes the unspecific signal amplification by HCR which increases selectivity and sensitivity. This is due to the fact that the hairpin of HCR is separated in being present in different arms of the Y-shaped probe. Under the optimal conditions, the assay has a limit of 0.45 pg·mL −1 for Kana. It was applied to analyze spiked milk, fish and pork samples.
The scheme represents a sensitive fluorometric aptamer-based method to detect kanamycin (Kana). It is making use of a double stirring bar-assisted dual amplification strategy with zero background. Abbreviations: apt: aptamer, AuNPs: gold nanoparticles, HCR: hybridization chain reaction.
研究领域:
分析化学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ailing Su,Yuan Liu,Xiumian Cao,Weiqing Xu,Chongyang Liang,Shuping Xu
A new gene detection platform based on surface-enhanced Raman spectroscopy (SERS) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system is developed, which is applied for virus gene detections. CRISPR/Cas12a activated by CRISPR ribonucleic acid (crRNA) can recognize and lock the complementary target double-stranded deoxyribonucleic acid (dsDNA), followed by the cis -cleavage on the dsDNA and the trans -cleavage on the nearby linker single-stranded DNA (ssDNA). Target-induced trans -ssDNA cleavage leads to a failure in an aggregation behavior of the designed SERS probe pair that was bridged by the linker ssDNA, which causes the SERS intensity reduced relative to the control trial that gives much stronger SERS due to the severe aggregation state of SERS probes caused by intact linker ssDNA. This sensing method achieves the highly sensitive detections of human papillomavirus (HPV) genes in serum and pseudovirus within 40 min. The detection sensitivity of this CRISPR/Cas-SERS platform can reach pM. Owing to the flexible designs of the DNA probe and crRNA, this CRISPR/Cas-SERS sensing system is a universal gene detection tool, which can be applied for the in-vitro diagnosis field and point-of-care testing.
研究领域:
分子生物学
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医学诊断
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生物传感技术
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 45
·25611-25616
·
IF 2.936
Dan Han,Chunying Wei
In this paper, we construct a fluorescence nucleic acid probe based on DNA-templated silver nanoclusters (DNA-Ag NCs) for the detection of the p53 gene. The fluorescence biosensing of the “turn-on” model is successfully implemented as a result of the target-triggered configurational change in the hairpin DNA probe and the synthesis of fluorescent Ag NCs. With this biosensor, the limit of detection (LOD) for the p53 gene is 3.57 nM and the linear range is 250–2500 nM in phosphate buffer solution, while a LOD of 6.06 nM in the linear range of 250–2500 nM is obtained in 1% diluted fetal calf serum. So this probe, with its advantages of specificity, practical application, easy operation and low cost, will have favourable development prospects in biological sensing and imaging.
研究领域:
生物化学
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分子生物学
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纳米生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Weiyi Lu,Bin Yang,Zihua Guo,Zhen Tian,Wenhui He,Tiancheng Yang
Phenothiazine drugs are widely used to treat psychotic diseases in humans. The phenothiazine ligand enables unique interaction between the drugs and DNA double helix. We discovered that it was impossible to select aptamer to selectively bind with phenothiazine drug. In this study, an alternative fluorescence sensor array was first developed to accurately discriminate four typical phenothiazine drugs based on machine learning. As the eight sensor elements, versatile DNA probes were designed based on different oligonucleotides , two unique fluorescence dyes , and diverse buffer solutions. The fingerprint data can offer the fluorescence response patterns against each phenothiazine drug. Linear discriminant analysis (LDA) can discriminate single one or mixture samples of phenothiazine drugs with 100% accuracy. Besides, one neural network model showed a good linear relationship between predicted and actual concentrations to quantitatively assay specific phenothiazine drugs. The proposed fluorescence sensor array presents a new way to accurately discriminate phenothiazine drugs in pharmacy or biology field.
研究领域:
生物化学
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药学
·
分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）