JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2015
·
IF 2.729
Shuang Ren,Huan Wang,Hongyi Zhang,Liqing Yu,Mingjie Li,Meng Li
Novel mesoporous carbon fibers (MCFs) were fabricated through a self-templating strategy and solution growth process. MCF modified glassy carbon electrode (MCF/GCE) was proposed for the direct electrocatalytic and simultaneous determination of four DNA bases (guanine (G), adenine (A), thymine (T) and cytosine (C)) using differential pulse voltammetry (DPV) measurement. The MCF/GCE exhibits satisfactory electrocatalytic activity by significantly enhancing the oxidation peak currents and lowering the oxidation potentials toward the four bases when compared with those on GCE. Especially, MCF greatly accelerates the electron transfer of pyrimidine base, which have high oxidation potentials and slow electron transfer kinetics. Moreover, MCF/GCE realizes the simultaneous determination of four DNA bases with low detection limits, high sensitivity and wide linear range. More important, the proposed electrode was successfully applied in the direct detection of bases in herring sperm DNA sample with satisfactory results. Besides, the MCF/GCE shows good long-term stability and reproducibility. These superior performances of MCF/GCE toward purine and pyrimidine DNA bases provide a good possibility for applying the MCF/GCE in our further DNA biosensor research.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Lijun Niu,Jianguo Li,Siqin Wang,Kyriakos Manoli,Lei Zhang,Xin Yu,Mingbao Feng
The transformation of extracellular DNA (e.g., antibiotic resistance genes) in water has attracted increasing attention over the last decades. To date, the effect of the resulting byproducts (e.g., DNA bases) on the degradation of micropollutants by oxidants remains largely unknown. In this study, four DNA bases (i.e., deoxyadenosine (dA), deoxythymidine (dT), deoxycytidine (dC), and deoxyguanosine (dG)) were investigated as potential permanganate (Mn(VII)) activators for the oxidation of tetracycline (TC) in water. The dC exhibited the best performance, e.g., the first-order rate constant of TC increased from 0.082 min −1 in the absence of dC (i.e., Mn(VII) alone) to 0.290 min −1 in the presence of 500 µM dC (i.e., Mn(VII)/dC system). The degradation of three other emerging micopollutants (i.e., bisphenol F (BPF), bisphenol S (BPS), and methotrexate (MTX)) by Mn(VII)/dC was also greater than Mn(VII) alone. Multiple lines of evidence suggested that the synergistic effect of in-situ formed MnO 2 colloids and Mn(VII) had a major role in the observed enhancement compared to radicals, Mn(VI), Mn(V) or Mn(III). The transformation products (TPs) of TC by Mn(VII) with and without dC were tentatively identified, from which the reaction pathways were proposed. Despite the high degradation of micropollutants by Mn(VII)/dC system, the majority of TPs were recalcitrant to biodegradation with multi-endpoint adverse health effects on different aquatic organisms, as indicated by in silico prediction software. Overall, this study unveiled the free DNA bases as in-situ Mn(VII) activators for removing emerging contaminants of global concern from water. However, the secondary environmental risks related to the TPs need to be considered in terms of water safety assessment.
研究领域:
环境科学
·
化学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2012
·
IF 4.555
Xi Ba,Liqiang Luo,Yaping Ding,Zhen Zhang,Yuliang Chu,Bijun Wang,Xiaoqian Ouyang
In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Mingyue Wang,Hao Guo,Rui Xue,Qixia Guan,Junye Zhang,Tingting Zhang,Lei Sun,Fan Yang,Wu Yang
Covalent organic frameworks (COFs) offer high surface area, ordered π conjugation system, tunable pore size and structure which are useful for developing electrochemical sensor. However, most COFs were poor conductivity and slow charge transfer rate which restrict their application in electrochemical sensor. Metal-covalent organic frameworks (MCOFs) possess the good electroconductivity and catalytic activity. In addition, the periodic arrays of π clouds of layered 2D structure facilitate charge transport. Here we propose a new strategy to construct a novel electrochemical sensor employing MCOFs (CuP-SQ COF). The fabricated sensor MWCNTs-COOH/CuP-SQ COF/Co NPs/GCE exhibits the excellent performance for the simultaneous determination of DNA base (guanine and adenine) with high sensitivity which can be attributed to the outstanding properties, unique structure and synergistic effect of composites. The wider concentration range of simultaneous quantitive analysis were 0.04 to 130 μM for guanine and 0.06 to 130 μM for adenine . The lower detection limits were 5.5 nM and 7.2 nM (S/N = 3), respectively. This work proves MCOFs can serve as a promising candidate as modified electrode material for developing electrochemical sensor.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
ELECTROANALYSIS
·
2018
·Vol. 30
·期 8
·1723-1733
·
IF 2.851
Xiaping Zhu,Yali Yuan,Yun Zhang,Jianping Li,Hongcheng Pan
Three different carbon materials, graphite, graphene and multiwalled carbon nanotubes (MWCNTs), were applied to fabricate carbon paste electrodes and used directly as working electrodes without any further modification in a simple electrochemical system for simultaneous detection of four DNA bases, guanine, adenine, thymine and cytosine. EIS and SEM were used to characterize the formed carbon paste electrodes made from different carbon nanomaterials and silicon oil, respectively. Conditions for bases detection were studied, such as ratio of carbon nanomaterials to silicon oil, types of buffer saline and pH. An unexpected result was discovered that compared with graphite and graphene, MWCNTs in carbon paste electrodes were not able to obtain admirable electrochemical behavior, the possible reason of which was preliminary discussed. Individual and simultaneous detection of four bases were successfully carried out, with acceptable linear ranges and low detection limits. Furthermore, this facile method had admirable reproducibility, stability and acceptable recovery in real urine sample (97.62 % ∼103.36 %), indicating certain practical potential.
研究领域:
生物化学
·
分析化学
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Jing Li,Xin Zhang,Wen-Yuan Fan,Mu-Cen Yao,Guo-Ping Sheng
Free DNA bases are widely present in the environments, and can be utilized by bacteria for their nucleic acids synthesis or as nutrition sources. In sunlit natural waters, these free bases probably undergo photodegradation which would change the bioavailable bases contents. Though the photodegradation of DNA has been investigated, the photodegradation behaviors of free bases may be quite different from those of DNA-confined bases in consideration of their different chemical environments. Herein, the photodegradation of four free bases (guanine, adenine , thymine and cytosine) was investigated. Results show that direct photodegradation of free bases in phosphate buffer caused by UV was slow. However, the photodegradation of these free bases were greatly enhanced in dissolved organic matter (DOM) solution. In the presence of 10–50 mg/L DOM, the photodegradation rates of free bases were increased by 1.85–14.6 times compared to the controls without DOM. DOM could result in indirect photodegradation by producing hydroxyl radical (•OH) and singlet oxygen ( 1 O 2 ) under irradiation, and this indirect photodegradation enhanced and dominated the free bases photodegradation. The •OH was involved in all four bases photodegradation, while the 1 O 2 only participated in guanine photodegradation. In phosphate buffer, the fastest photodegradation bases were pyrimidine, however, guanine became the fastest photodegradation base in DOM solution due to the selective oxidation of guanine by 1 O 2 . In summary, DOM may be a determinant for free bases photodegradation in natural waters and thereby deeply influence free bases fates in aquatic environments.
研究领域:
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Guohua Qi,Dandan Wang,Chuanping Li,Kongshuo Ma,Ying Zhang,Yongdong Jin
Molecular diagnosis and accurate damage analysis of complex genomic DNAs in tumor cells are crucial to the theranostics of cancers but still a huge challenge. Herein, by designed preparation of a uniform plasmonic sunflower-like assembly gold (Au) nanostructure that is capable of efficient DNA capture and providing high-density gap-plasmon “hot spots” for adequate surface-enhanced Raman spectroscopy (SERS) enhancement, we succeeded in sensitive and reliable label-free SERS detection of DNA damage in electrostimulus-induced apoptotic cancer cells at the DNA base level for the first time. The SERS results showed that the external electrostimulus (at 1.2 V, for 5 min) was almost harmless to normal healthy cells, but it caused pronounced double strand break and adenine base damage in cancer cell DNAs, which effectively destroyed the reproduction and transcription of DNAs and ultimately induced cell apoptosis. The developed sensing platform and method are promising for cell study of genetically related diseases.
研究领域:
纳米技术
·
生物医学工程
·
癌症研究
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Peng Lei,Ying Zhou,Bei Li,Yang Liu,Chuan Dong,Shaomin Shuang
The unique combination of DNA bases is essential for storing genetic information. A reliable and accurate electrochemical sensor based on a gold/palladium–polypyrrole/graphene nanocomposite (Au/Pd–PPy/GR) has been prepared to individually and simultaneously detect guanine (G), adenine (A), thymine (T), and cytosine (C). The bimetallic nanomaterial (Au/Pd–PPy) is used to enhance the sensing performance of DNA bases, and the GR could further increase the electroactive area and amplify the electrochemical response. As expected, the electrochemical sensor shows excellent electrocatalytic activity with well-separated current responses at 0.704 V (G), 0.976 (A), 1.164 (T), and 1.348 V (C). The prepared sensor exhibits the linear range of 1–350 μM for G, 5–300 μM for A, 20–900 μM for T, and 10–700 μM for C. The developed device is also demonstrated as an excellently sensitive and highly selective sensor to identify the four bases in calf thymus DNA, which provides the application of electrochemical sensors in bioscience.
研究领域:
生物化学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Yating Li,Jason Tan,Yanqin Tu,Jingnan Wu,Zaifang Zhang,Yifeng Wei,Xinan Jiao,Yan Zhou
DNA base modification is a common strategy used by bacteriophages to evade host immune detection. A prominent example is dU-DNA, where thymidine is globally replaced with 2′-deoxyuridine. Despite its widespread occurrence, dU-DNA's biosynthetic pathways and functional roles remain incompletely understood. Here, different enzymes supporting dU-DNA biosynthesis in phage PBS1, Roseophage DSS3_VP1, and Yersiniophage PhiR1-37 are identified and characterized. The dU nucleotide precursor is supplied by phage dCTP deaminases (Dcds). Thymidine nucleotides are degraded by dTMP phosphatases (Dtms) in PBS1 and DSS3_VP1, and by a dTTP pyrophosphatase (Dtt) in PhiR1-37, preventing incorporation into phage DNA. The dU-DNA, isolated from Yersiniophage PhiR1-37 or synthesized by PCR, demonstrates resistance to cleavage by restriction enzymes recognizing thymidine-containing sequences, partial resistance to Lb Cas12a nuclease recognizing a TTTV PAM site, while remaining sensitive to Sp Cas9 nuclease recognizing a NGG PAM site. A phylogenetic analysis of PBS1 dCTP deaminase and closely related T4 phage dCMP deaminase suggest possible evolutionary origins from bacterial dCDP deaminases. Overall, these findings suggest independent acquisition of dU-DNA biosynthetic enzymes and pathways in the diverse phages, and support its protective function against different host-encoded nucleases.
研究领域:
分子生物学
·
病毒学
·
微生物学
·
基因工程
·
核酸代谢
·
免疫逃避
·
进化生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 136
·期 21
·4480-4485
·
IF 3.913
Shujuan Xu,Yong Shao,Kun Ma,Qinghua Cui,Guiying Liu,Fei Wu,Minjie Li
DNA single-nucleotide polymorphism (SNP) detection has attracted much attention due to mutation-related diseases. Various fluorescence methods for SNP detection have been proposed and many are already in use. However, fluorescence enhancement for signal-on SNP identification without label modification still remains a challenge. Here, we find that the abasic site (AP site) in a DNA duplex can be developed as a binding pocket favorable for the occurrence of the excited-state intramolecular proton transfer (ESIPT) of a 3-hydroxyflavone, fisetin, which is used as a proof of concept for effective SNP identification. Fisetin binding at the AP site is highly selective for target thymine or cytosine facing the AP site by observation of a drastic increase in the ESIPT emission band. In addition, the target recognition selectivity based on this ESIPT process is not affected by flanking bases of the AP site. The binding selectivity of fisetin at the AP site is also confirmed by measurements of fluorescence resonance energy transfer, emission lifetime and DNA melting. The fluorescent signal-on sensing for SNP based on this fluorophore is substantially advantageous over the previously used fluorophores such as the AP site-specific signal-off organic ligands with a similar fluorescing mechanism before and after binding to DNA with hydrogen bonding interaction. We expect that this approach will be employed to develop a practical SNP detection method by locating an AP site toward a target and employing an ESIPT probe as readout.
研究领域:
化学
·
生物化学
·
分子生物学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2020
·Vol. 30
·期 49
·2002239
·
IF 16.836
Wuyang Yu,Qian Cheng,Jingjie Ye,Mingkang Zhang,Cheng Zhang,Fan Gao,Xinglan Ding,Jun Feng,Xian-Zheng Zhang
To advance clinical translation of antitumor nanomedicines, the development of a universally applicable, facile, and safe methodology for nanomedicine construction to overcome major hurdles across tumor-specific nano-delivery in an all-in-one manner would be highly desirable. This study puts forward a HAylation strategy based on positively charged nanocore (NC + ) and hyaluronic acid (HA) that is naturally present in the human body. HAylation leads to prolonged blood duration and active tumor targeting. It is demonstrated that HAylation facilitates not only selective uptake of nanomedicines by tumor cells but also deep tumor infiltration via the special transcytosis pathway. This characteristic is ascribed to the site-specific exposure of NC + in tumor cells due to fast HA degradation exclusively in response to HAase and an acidic pH in the lysosome after tumor accumulation. HAylation enables the free construction of a NC + nano-core with versatile functions. Here, a NC + core is fabricated by directly assembling a photodynamic therapy (PDT) agent and an inhibitor of the DNA base excision repair (BER) pathway. The inhibition of BER-mediated DNA repair has proven powerful to amplify antitumor efficacy since DNA damage is one major cell-killing mechanism for many anticancer therapeutics. HAylation holds promise for the simplification of the industrial production of nanomedicines and arms nanomedicines to thoroughly overcome fundamental nano-delivery hurdles.
研究领域:
纳米医学
·
癌症治疗
·
药物递送系统
·
分子生物学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Chen-Yu Xue,Ya-Hong Liu,Yue Yu,Ying Liu,Ying-Lin Zhou,Xin-Xiang Zhang
The apurinic/apyrimidinic (AP) site is an important intermediate in the DNA base excision repair (BER) pathway, having the potential of being a biomarker for DNA damage. AP sites could lead to the stalling of polymerases, the misincorporation of bases and DNA strand breaks, which might affect physiological function of cells. However, the abundance of AP sites in genomic DNA is very low (less than 2 AP sites/10 6 nts), which requires a sensitive and accurate method to meet its detection requirements. Here, we described an ultrasensitive quantification method based on a hydrazine- s -triazine reagent (i-Pr 2 N) labeling for AP sites combining with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The limit of detection reached an ultralow level (40 amol), realizing the most sensitive MS-based quantification for the AP site. To guarantee the accuracy of the quantitative results, the labeling reaction was carried out directly on DNA strands instead of labeling after DNA enzymatic digestion to reduce artifacts that might be produced during the enzymatic process of DNA strands. And selective detection was realized by MS to avoid introducing the false-positive signals from other aldehyde species, which could also react with i-Pr 2 N. Genomic DNA samples from different mammalian cell lines were successfully analyzed using this method. There were 0.4–0.8 AP sites per 10 6 nucleotides, and the values would increase 16.1 and 2.75 times when cells were treated with genotoxic substances methyl methanesulfonate and 5-fluorouracil, respectively. This method has good potential in the analysis of a small number of cell samples and clinical samples, is expected to be useful for evaluating the damage level of DNA bases, the genotoxicity of compounds and the drug resistance of cancer cells, and provides a new tool for cell function research and clinical precise treatment.
研究领域:
分子生物学
·
分析化学
·
毒理学
·
药理学
·
临床研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）