Every year, a significant amount of fruit and vegetable spoilage caused by microbial contamination leads to substantial food waste and economic losses in the agricultural and food sectors. There is an urgent need to develop highly efficient and safe multifunctional preservation materials. Curcumin (Cur) is known for its excellent antioxidant and antibacterial properties; however, its poor water solubility and low bioavailability severely limit its practical applications. In this study, we synthesized highly stable and water-dispersible metal-biomolecule network nanoparticles by self-assembly of ferrous ions (Fe 2+ ) with DNA in an aqueous solution at room temperature. Furthermore, by leveraging the strong coordination interaction between Iron(Fe) and Cur, we prepared Cur-modified nanoparticles (Fe-DNA-Cur) with dual antioxidant and antibacterial functions. Fe-DNA-Cur not only preserves the biological activity of curcumin, but also effectively addresses its inherent poor water solubility. After further compounding with chitosan (CS), the as-prepared Fe-DNA-Cur@CS composite demonstrates breakthrough antimicrobial performance, thereby providing an innovative solution for developing novel, highly effective and safe fruit/vegetable preservatives. When used as a sustainable food packaging coating for perishable fruits at room temperature, the Fe-DNA-Cur@CS coating can reduce dehydration, prevent nutrient loss, inhibit microbial growth, increase nutraceutical value, maintain strawberry quality, and extend shelf life during storage. This study demonstrates the potential of Fe-DNA-Cur@CS as a multifunctional coating material to reduce food waste and agricultural economic losses caused by microbial contamination.
Qingqing Zhang,Dandan Hu,Tianyu Zhan,Yufang Hu,Zhiyong Guo,Sui Wang
A novel Medusa electrochemiluminescence (ECL) immunosensor for quantitative detection of histone acetyltransferase (HAT p300) is designed and built well based on magnetic gold nanoparticles (Au-Fe 3 O 4 NPs), DNA nanonets and terminal deoxynucleotidyl transferase (TdT)-triggered renascent DNAs containing abundant A and T (named as AT-dsDNAs). Firstly, Au-Fe 3 O 4 NPs and substrate peptides are coated with the bare electrode surface one after the other. As the acetylation reaction happens, DNA nanonets, which are synthesized via Y-shape DNA (as building block) and acetyl antibody (Ab Ac )-grafted dsDNA (as crosslinker), are attached on the electrode surface. Next, AT-dsDNAs are formed under NaCl condition by TdT catalysis, in where deoxyadenosine triphosphate (dATP) and deoxythymidine triphosphate (dTTP) (half and half) can be added ceaselessly to countless 3′−OH of DNA nanonets. Abundant Ru(phen) 3 2+ molecules, which can be integrated well into high-level DNA nanomaterials above, serve as ECL luminophore in our biosensing. Only being HAT p300, an outstanding ECL response occurs favorably, and a detection limit of 0.0029 nM is acquired with a linear response in the range of 0.006–60 nM. The Medusa immunosensor with improved sensitivity presents a promising and possible widely use for the HATs-relevant clinical study and drug development.
危险品化学品经营许可证（带存储）