SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Ying Zhang,Qian Liu,Mengting Liu,Xueni Zhang,Xiaoya Li,Lihui Dai,Meng Meng,Dingbin Liu,Yongmei Yin,Rimo Xi
The microenvironment of lysosomes plays an important role in various functions within cells. Abnormal pH changes in lysosomes account for multiple diseases including tumors. Acid-responsive nanomaterials with reconfigurable structures and properties have garnered significant interests. Owing to the potential in biosensing, controlled release and nanomachinery, DNA based pH-responsiveness has been widely pursued. Herein, we designed an AuNPs-i-motifs based SERS probe to monitor the pH dynamics of lysosomal maturation in different cells. Taking advantages of biocompatibility and structural flexibility of DNA, unique optical and plasmonic properties of gold nanoparticles , excellent stability and enhanced sensitivity of background-free Raman reporter, the designed SERS probe could indicate the pH variation dynamically in lysosomes. What is more valuable is that 4-mercaptobenzonitrile is not only used as a Raman reporter to provide sensitive Raman signal for pH sensing, but also used as a sealing agent allowing for controllable aggregation, to improve the stability and accuracy of the sensor. With this versatile design, the sensor was prepared simply and feasibly and a tedious trial was avoided. It provides a feasible tool for understanding the relationship of lysosomal pH with fundamental cellular functions and mechanism of diseases.
研究领域:
生物传感
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纳米医学
·
细胞生物学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Hao Hu,Jinyi Zhang,Yu Ding,Xinfeng Zhang,Kailai Xu,Xiandeng Hou,Peng Wu
Singlet oxygen (1O2), generated via photosensitization, has been proved to oxidize chromogenic substrates with neither H2O2 oxidation nor enzyme (horseradish peroxidase, HRP) catalysis. Of the various methods for modulation of the 1O2 generation, DNA-controlled photosensitization received great attention. Therefore, integration of the formation/deformation DNA structures with DNA-controlled photosensitization will be extremely appealing in visual biosensor developments. Here, the stable melamine-thymine complex was explored in combination with DNA-controlled photosensitization for visual detection of melamine. A T-rich single stand DNA was utilized as the recognition unit. Upon the formation of the T-M-T complex, double stand DNA was formed, which was ready for the binding of SYBR Green I and activated the photosensitization. Subsequent oxidation of TMB allowed visual detection of melamine in dairy products, with spike-recoveries ranging from 94% to 106%.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
Science Advances
·
2025
·Vol. 11
·期 14
·
IF 12.5
Yinong Li,Yawei Wen,Leticia C. Beltrán,Li Zhu,Shishan Tian,Jialong Liu,Xuan Zhou,Piaoyi Chen,Edward H. Egelman,Ming Zheng,Zhiwei Lin
DNA has demonstrated the abilities to differentiate single-wall carbon nanotubes (SWCNTs) with various chiralities and manipulate their analyte sensing properties. However, the fundamental mechanisms underlying these remarkable abilities remain unclear due to the lack of high-resolution determination of DNA structures on SWCNTs. Here, we combine atomic force microscopy and single-particle cryo–electron microscopy to determine DNA structures on five different types of single-chirality SWCNTs, achieving unprecedented subnanometer resolution. This resolution enables the direct observation of left-handed helical DNA structures with pitches ranging from 1.59 to 2.20 nm, depending on the DNA sequence and nanotube chirality. These findings provide structural insights into the mechanisms by which DNA differentiates the chirality of SWCNTs, and governs the sensitivity, dynamic response range, and analyte differentiability of SWCNT sensors. We propose a non–Watson-Crick hydrogen-bonding network model, which not only accounts for the observed ordered DNA structures but also facilitates the design of DNA sequences for targeted SWCNT purification and desired SWCNT sensor performance.
研究领域:
纳米生物学
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结构生物学
·
材料科学
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DOI
PubMed
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-10
·
IF 12.353
Li Xinchun,Zhai Tianyou,Gao Pengcheng,Cheng Hongli,Hou Ruizuo,Lou Xiaoding,Xia Fan
Nanochannels with functional elements have shown promise for DNA sequencing, single-molecule sensing, and ion gating. Ionic current measurement is currently a benchmark, but is focused solely on the contribution from nanochannels’ inner-wall functional elements (NIWFE); the attributes of functional elements at nanochannels’ outer surface (NOSFE) are nearly ignored, and remain elusive. Here we show that the role of NOSFE and NIWFE for ion gating can be distinguished by constructing DNA architectures using dual-current readout. The established molecular switches have continuously tunable and reversible ion-gating ability. We find that NOSFE exhibits negligible ion-gating behavior, but it can produce a synergistic effect in alliance with NIWFE. Moreover, the high-efficiency gating systems display more noticeable synergistic effect than the low-efficiency ones. We also reveal that the probe amount of NOSFE and NIWFE is almost equally distributed in our biomimetic nanochannels, which is potentially a premise for the synergistic ion-gating phenomena.
研究领域:
生物物理学
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纳米技术
·
分子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-11
·
IF 6.232
He Liyong,Shen Zhipeng,Wang Jiaqi,Zeng Jin,Wang Wenhai,Wu Huihui,Wang Qiqin,Gan Ning
The authors describe a microfluidic chip-based aptasensor platform combined with magnetic tripartite DNA structure-functionalized nanocomposites to achieve simultaneous determination of kanamycin (KANA), aflatoxin M1 (AFM1), and 17β-estradiol (E2) in milk. The two-duplex tripartite DNA nanostructure was first assembled on the surface of magnetic beads. When the aptamer on the probes recognized the specific target, the aptamer-target would be released into the supernatant. The pre-primer@circular DNA template structure initiates rolling circle amplification (RCA) by phi29 polymerase. After magnetic separation, the magnetic nanocomposites were added into a solution containing three different lengths of complementary strands to the RCA products. The number of complementary strands significantly decrease, and this can be quantitated by the microfluidic chip. Further, the employment of magnetic nanocomposites and microfluidic chip not only resolve the complex matrix interference, but also dramatically enhances the determination selectivity and sensitivity. This aptasensor allows for determination of KANA, AFM1, and E2 with limits of detection as low as 0.32 pg mL −1 , 0.95 pg mL −1 , and 6.8 pg mL −1 , respectively. This novel method exhibits the advantages of excellent stability and fast response time (< 3 min on microfluidic chip platform) for simultaneous determination of KANA, AFM1, and E2 in milk samples and ensures food safety.
Graphical abstract
研究领域:
分析化学
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食品安全
·
生物传感技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Ting Li,Shaojiao Song,Wei Lu,Yu Cao,Xin Zheng,Qiuyue Zhao,Hailong Wang
Owing to the high selectivity and binding affinity of coralyne (COR) with deoxyribonucleic acid (DNA)/ribonucleic acid, COR has been confirmed as an effective anticancer drug. However, a direct and sensitive detection assay for a DNA–COR–DNA structure with a portable instrument has not been reported because of the lack of a high-resolution sensor that can identify DNA–COR interaction at a single-molecule level. Here, specific Types I and II signals generated by a DNA–COR–DNA duplex were observed using a versatile α-hemolysin nanopore sensor. The Type I signal featuring a unique current pattern was preferentially selected via DNA sequence design and voltage control. Subsequently, the rapid and sensitive detection of COR was established with a detection limit of 0.1 nM. The pH environment, observed to influence the binding of the DNA–COR–DNA duplex, was significant for drug release controlling during targeted delivery process. Similar alkaloids were tested, and the nanopore sensor exhibited a high selectivity for COR. The results demonstrate a potential platform for investigating DNA-COR interaction and could be applied in the drug therapy and pharmacodynamic studies of COR.
研究领域:
生物物理学
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分子生物学
·
药理学
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DOI
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Huihui Chen,Yuhan He,Qijie Wang,Xinyao Yi,Jianxiu Wang
Ultraviolet (UV) irradiation can destroy hydrogen bonds, leading to DNA deformation or destruction. Triplex DNA is produced by combining Watson–Crick and Hoogsteen base pairing under certain conditions. The UV-induced damage of target DNA may influence the triplex DNA formation; thus, a simple and sensitive surface plasmon resonance (SPR) assay of the DNA damage process was proposed. The experimental conditions for forming the triplex DNA were explored, and the stability comparison between duplex and triplex DNA was performed. The triplex DNA possessed higher stability under acidic pH and sufficient Mg2+, and the sequence length could remarkably influence its stability. By derivatization of the triplex structure with two biotin tags, the incorporation of streptavidin resulted in amplified SPR signals. However, UV-induced damage of biotinylated target DNA attenuated the triplex DNA formation, and smaller SPR signals were attained. The SPR signals were inversely and linearly dependent on the UV irradiation doses that represented the photodamage levels in the range of 0.117–1.75 kJ/m2, and the detection limit was estimated to be 0.039 kJ/m2. Through the competitive assay, UV-induced damage of unbiotinylated target DNA can also be detected. The proposed method serves as a viable means for the detection of UV-induced DNA damage based on the triplex DNA structure.
研究领域:
生物化学
·
分子生物学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Lina Wang,Xiangbiao Zeng,Li Zhang,Ying Yu,Bixia Lin,Yumin Wang,Manli Guo,Yujuan Cao
Herein, a novel filed-free electrochemical sensor based on inverted Y-type DNA conformation was developed for the ultrasensitive determination of tetracycline (TC). In this sensor, a ferrocene was immobilized on a DNA strand near the interface and showing a signal as reference. Meanwhile, a methylene blue (MB) was modified on a DNA vertical away from the interface, presenting a little signal. When the target TC attracted the aptamer, the DNA modifying MB was released, and then exchanged another DNA near the interface via toe-hold strand displacement effect. This caused an obvious enhancement of the MB signal, and achieved a sensitive response to the TC amount. In comparison to other DNA sensors based on field-confined assembling mode, this sensor exploited the transform of self-structure to make signal response, which avoids the steric hinerance effect and achieving field-free conversion. Meanwhile, the immobile ferrocene provided a reference proportion, reducing the influence of nonspecific signal on measurement accuracy. The followed assays demonstrated when the base-pairs on the straight side were 1.5-fold to that on the hypotenuse, the strand transferring efficiency and signal response were optimal. It exhibited a linearity of 1.0 × 10 -1 –1.0 × 10 3 nM for TC with a detection limit of 28.4 pM. In practical assay, the measured result was matched to that of the contrast method. Meanwhile, the recovery rates ranged from 98.0% to 103.0% with the RSD of 1.4%∼2.5%, respectively. Indicating an expectant application prospect of the proposed sensor in antibiotic monitoring.
研究领域:
生物化学
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分析化学
·
环境监测
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jianhao Hu,Zhipeng Shen,Lei Tan,Jun Yuan,Ning Gan
In this work, we demonstrate a double stirring bars-based signal-amplified strategy for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T). Methylene blue (MB) or ferrocene (Fc) labeled functionalized branched-chain DNA hybrid structure (FBCHS) was immobilized on magnetic microspheres and used as encoded signal tags. The encoded signal tags were hybridized with the aptamer-embedded tetrahedral DNA nanostructures (TDNs), which acted as capture probes, on one gold stirring bar (bar-A). The magnetic signal tags on bar-A could capture V.P and S.T, and their complex was later released into the solution. The composites were then digested by exonuclease I that was modified on another gold stirring bar (bar-B). The released free bacteria participated in the next cycle, from bar-A to bar-B, and generated more magnetic tags for signal amplification. After the reaction, the tags were magnetically enriched on a screen-printed carbon electrode (SPCE), generating two distinct voltametric peaks derived from MB and Fc. Their current intensities could reflect the concentration of V.P and S.T in the samples. Under optimal conditions, the double stirring bars-based signal amplification strategy was able to increase the detection sensitivity with a lower detection limit of 4 and 7 CFU/mL for V.P and S.T, respectively, within a detection range of 10–10 8 CFU/mL. Importantly, the exonuclease I on the stirring bar (bar-B) for signal amplification was able to be reused up to 50 times and avoided its loss if being directly added in solution, which produced cost savings in terms of used enzymes.
研究领域:
生物技术
·
食品科学
·
分子生物学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Zhenjie Qiao,Yongcun Yan,Sai Bi
The monitoring of blood glucose levels is essential to the diagnosis and management of diseases, especially diabetes. Thus, it is of great importance to develop a simple, accurate and sensitive method for glucose detection. Herein, the three-dimensional DNA flowers-templated Ag nanoclusters (DFs/AgNCs) with enhanced fluorescent brightness and good stability are in situ synthesized via rolling circle amplification (RCA), which are further successfully applied for the construction of fluorescent/electrochemical dual-mode glucose biosensor . In this assay, the AgNCs encapsulated in DFs can be etched by H 2 O 2 that is generated from the process of glucose oxidase (GOx)-catalyzed glucose oxidation , resulting in the release of Ag + to facilitate the signal-off fluorescent detection of glucose. Based on the coordination interaction of cytosine-Ag + -cytosine (C-Ag + -C), the liberated Ag + further induces the conformation switch of ferrocene (Fc)-labeled DNA capture probe (Fc-DNA) with C-rich sequence on Au electrode, leading to the signal-on electrochemical detection of glucose. The proposed DFs/AgNCs-based fluorescent/electrochemical dual-mode biosensor has achieved the sensitive and selective detection of glucose in human serums with good accuracy and reliability, which not only provides a rapid, reliable and accurate method for glucose detection but also opens a new avenue to the development of DNA nanotechnology-based biosensing strategies.
研究领域:
生物传感技术
·
纳米医学
·
分析化学
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产品关联
DOI
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 45
·21856-21866
·
IF 6.97
Hongmei Geng,Chunyang Zhou,Chunlei Guo
In this paper, we propose a strategy involving coupling DNA structural nanoswitches with toehold mediated strand displacement for constructing novel DNA-based digital comparator (DC) logic systems, which are a basic part of traditional electronic computers and can compare whether two or more input numbers are equal. However, when the number of DC inputs is increased to a certain level, the speed and quality of the computing circuit can be affected because of the limitations of conventional electronic computers when it comes to handling large-scale quantities of data. To solve this problem, in this work, we introduce a multi-input to multi-output DNA switch-based platform that can enable complex DC logical comparison. These multifunctional DNA-based switches, each including two hairpin-shaped molecular beacons and a G4/NMM complex, were used as platforms for the step-by-step realization of 2-3 DC, 3-3 DC, and 4-3 DC logic operations. Also, experiments were designed to further verify the excellent selectivity, achieving single-base mismatch operations with the digital comparator. Based on our design, comparators (“>”, “<” and “=”) can be realized. Our prototype can inspire new designs and have intelligent digital comparator and in-field applications.
研究领域:
生物化学
·
分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Chuang Zhang,Xinhua Zhang,Jian Zhang,Mian Peng,Xiaowen Dou
Circulating tumor cells (CTCs), though rare and highly heterogeneous, are closely associated with tumor initiation, progression, and prognosis. Their low abundance, structural fragility, phenotypic diversity, and size variability make efficient enrichment particularly challenging. To address these limitations, we developed a novel CTC isolation platform that combines tetrahedral DNA nanostructure-based trivalent aptamer magnetic nanospheres (TDF-Cocktail-MNP) with a magnetic microfluidic separation device. This system enhances the capture of viable, phenotypically diverse CTCs and supports reliable downstream analysis. The TDF structure presents three aptamers: EpCAM targets epithelial CTCs, Vimentin captures mesenchymal types, and EGFR binds to cells overexpressing EGFR—a key marker in triple-negative breast cancer. This multi-target approach enables efficient isolation of heterogeneous CTC populations from whole blood samples. Captured CTCs are enriched using the IsoFlux System, which reduces mechanical stress and preserves cell viability by avoiding repeated magnetic manipulation. After nuclease-mediated removal of the DNA scaffold, cells are classified and quantified through immunocytochemistry. Validated with both spiked and clinical breast cancer samples, this platform shows strong potential for liquid biopsy applications, offering robust CTC detection and valuable insights for personalized cancer diagnostics and treatment.
研究领域:
肿瘤学
·
分子生物学
·
纳米医学
·
转化医学
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生物材料
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液体活检
·
微流控技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）