Nanoscale
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2019
·Vol. 11
·期 11
·5048-5057
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IF 6.97
Siqi Zhang,Kai-Bin Li,Wei Shi,Jie Zhang,De-Man Han,Jing-Juan Xu
The integration of multi-level DNA logic gates for biological diagnosis is far from being fully realized. In particular, the simplification of logical analysis to implement advanced logic diagnoses is still a critical challenge for DNA computing and bioelectronics. Here, we developed a magnetic bead/DNA system to construct a library of logic gates, enabling the sensing of multiplex target miRNAs. In this assay, the miRNA-catalyzed hairpin assembly (CHA) was successfully applied to construct two/three-input concatenated logic circuits with excellent specificity extended to design a highly sensitive multiplex detection system. Significantly, the CHA-based multiplex detection system can distinguish individual target miRNAs (such as miR-21, miR-155, and miR let-7a) under a logic function control, which presents great applications in the development of rapid and intelligent detection. Another novel feature is that the multiplex detection system can be reset by heating the output system and the magnetic separation of the computing modules. Overall, the proposed logic diagnostics with high amplification efficiency is simple, fast, low-cost, and resettable, and holds great promise in the development of biocomputing, multiparameter sensing, and intelligent disease diagnostics.
研究领域:
生物信息学
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生物医学工程
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分子生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Materials Horizons
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2018
·Vol. 6
·期 2
·375-384
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IF 13.183
Daoqing Fan,Erkang Wang,Shaojun Dong
DNA computing has shown impressive developments and exhibited amazing power over the past few decades. However, most current DNA logic devices can only produce single-modal output that is monochromic or even invisible, which greatly limits their reliability and practicality, integration capability and computing complexity. Emission-tunable upconversion nanoparticles (UCNPs) are ideal multicolor outputs for DNA computing, however UC luminescent (UCL) DNA logic systems have rarely been reported. Here, for the first time, we report “DNA-Unlocked Inner-Filter-Effect” (DU-IFE) between oxidized 3,3′,5,5′-tetramethylbenzidine (OxTMB) and upconversion nanoparticles (UCNPs) that subtly matches a “lock–key” strategy. Based on the DU-IFE, we construct the first ever UCL DNA logic library. Superior to previous DNA logic systems, this logic library can generate dual-modal (visual and UCL) multicolor label-free outputs, in which RGB (red, green, blue) colorful UCL ones are also visual under harmless NIR-laser. By selectively unlocking the IFE “lock” with different DNA “keys”, diverse basic, advanced and concatenated logic circuits, a logic-controlled molecular RGB-signal-light, triple-parallel-arrays are successfully constructed. All of the above logic operations demonstrate the strengthened reliability and practicality, powerful integration capability and improved computing complexity of this UCL logic library. Moreover, we fabricate an inspiring and useful biosensor with RGB colorful results based on the DU-IFE (a DNA caliper), providing a vivid prototype for potential biological applications.
研究领域:
生物化学
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分子生物学
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纳米技术
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生物医学工程
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DOI
高影响因子
TALANTA
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2018
·
IF 4.244
Qiang Zou,Xin Li,Tao Xue,Jia Zheng,Qi Su
A conceptually new class of DNA logic gates for Hg 2+ and Pb 2+ ion simultaneous detection has been constructed based on the activity of DNAzyme dependent on metal ions . For sensitive and specific detection of Hg 2+ and Pb 2+ ions, we demonstrate a surface-enhanced Raman scattering (SERS)-active platform by employing the oligonucleotide-functionalized and gold coated polystyrene microspheres (PSMPs). In this work, Hg 2+ and Pb 2+ were used as the input, and the SERS signals were the output. Hg 2+ ions were captured by a mismatched T-Hg-T bridge. Pb 2+ ions were bound to 8–17DNAzyme to break the cleavage substrate. The limit of detection (LOD) of Hg 2+ is 0.1 nmol L −1 and Pb 2+ is 1.0 nmol L −1 , which is far below the limit of 10.0 ppb (31 nmol L −1 ) and 10.0 ppm (3.02 × 10 4 nmol L −1 ) for drinking water stipulated by the World Health Organization. Additionally, this system has the potential to be ideal candidates for intelligent DNA calculator design applications. Furthermore, the designed strategy could be extended to other multiplex metal ions that can be combined with DNA molecules, and can be applied to many other fields such as environmental detection, toxin detection and liquid analysis.
研究领域:
分析化学
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环境科学
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生物化学
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纳米技术
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DOI
PubMed
高影响因子
Chemical Science
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2019
·Vol. 10
·期 30
·7290-7298
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IF 9.556
Daoqing Fan,Juan Wang,Erkang Wang,Shaojun Dong
Although DNA computing has exhibited a magical power across diverse areas, current DNA logic gates with different functions are always separately operated and can only produce hard-to-visualize output. The fussy/obligatory gates' redesign/reconstruction and the non-intuitive output cause the wastage of time and costs, low efficiency and practicality. Herein, inspired by the ancient Roman mythical God Janus, for the first time, we propose the concept of “DNA Janus Logic Pair” (DJLP) to classify the DNA logic gates with contrary functions into “Positive + Negative” gates (DJLP = Pos + Neg). Based on the biocatalytic property of G-quadruplex DNAzyme (G4zyme) and the luminescence quenching ability of oxidized 3,3′,5,5′-tetramethylbenzidine (OxTMB) towards the upconversion (UC) particles, we fabricated a universal amphichromatic platform that kills two birds with one stone for operating a versatile DJLP library. Different from the previous DNA logic systems, the “Pos + Neg” gates of each DJLP in this study were concomitantly achieved via the same one-time DNA reaction, which avoided the gates' redesign/reoperation and reduced the operating costs/time of the DNA gates by at least half. Besides, both the amphichromatic outputs (Visual-blue and UC luminescent-green) can be visualized under harmless-NIR, thus bringing greatly enhanced practicality to the method. Moreover, we constructed various concatenated logic circuits via logically modulating the G4zyme's biocatalytic property with glutathione, thus enabling the largely improved computing complexity. Furthermore, taking the circuit “YES-INH-1-2 decoder” as the “computing core”, we designed an “antioxidant indicator” with ratiometric logical responses that could recognize the presence of antioxidants smartly (output changed from “10” to “01”), which provided a typical prototype for potential intelligent bio-applications.
研究领域:
生物信息学
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分子生物学
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化学生物学
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纳米技术
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DOI
PubMed
高影响因子
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