CHINESE CHEMICAL LETTERS
·
2021
·
IF 6.779
Yuan Liu,Gaojian Yang,Taotao Li,Yan Deng,Zhu Chen,Nongyue He
An improved ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX) was applied to select aptamers against carbaryl. After nine selection rounds, a highly enriched ssDNA pool was obtained. The Apta3 was demonstrated as the optimal aptamer. In order to facilitate the modification of aptamer, the Apta3 was further truncated with the dissociation constant ( K d ) of 0.364 ± 0.055 μmol/L and a fluorescent aptasensor was developed. The linear range for carbaryl was from 100 nmol/L to 1500 nmol/L, with the limit of detection was as low as 15.23 nmol/L. Besides, the biosensor was validated for the carbaryl spiked real samples, and the recoveries were between 97.7% and 107.3%.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Weiwei Qiu,Feng Gao,Natsumi Yano,Yusuke Kataoka,Makoto Handa,Weiqiang Yang,Hidekazu Tanaka,Qingxiang Wang
Electrochemical aptasensors involved in chemical labeling are often single-use and sensitivity-limited because the probes are commonly single-point labeled and irreversible. In this work, the specific coordination between Zr4+ and phosphate group (−PO43–) was employed to construct a new aptasensor that is highly sensitive and reusable, using Ochratoxin A (OTA) as the test model. The OTA binding aptamer (OBA) was hybridized with the thiolated supporting sequence (TSS) immobilized on the surface of a gold electrode. The UiO-66 with a formula of [Zr6O4(OH)4(BDC)6], one of the class of Zr metal–organic frameworks (MOFs), was then particularly grafted on the terminal of OBA through the specific coordination between Zr4+ and 5′-PO43–, i.e., the Zr–O–P coordination bond. Similarly, as much as the 5′-PO43– and 3′-methylene blue dual-labeled sequences (DLS) were further assembled on UiO-66 due to the large surface area of MOF and rich active sites of Zr4+. Owing to the specific coordination for signal amplification, the developed aptasensor shows greatly enhanced sensitivity. A wide detection range from 0.1 fM to 2.0 μM and an ultralow detection limit of 0.079 fM (S/N = 3) for OTA were obtained. Additionally, the TSS can rehybridize with a new OBA to regenerate the aptasensor but without complicated pretreatments, enabling a aptasensor that is readily reusable for OTA detection. The aptasensor was successfully applied for OTA detection in the red wine samples, demonstrating a promising prospect for food safety monitoring.
研究领域:
生物化学
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食品安全检测
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纳米材料科学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Shijia Wu,Nuo Duan,Weixiao Zhang,Sen Zhao,Zhouping Wang
Patulin (PAT) is a kind of mycotoxin that has serious harmful impacts on both food quality and human health. A high-affinity ssDNA aptamer that specifically binds to patulin was generated using systemic evolution of ligands by exponential enrichment (SELEX) assisted by graphene oxide (GO). After 15 rounds of positive and negative selection, a highly enriched ssDNA pool was sequenced and the representative sequences were subjected to binding assays to evaluate their affinity and specificity. Of the eight aptamer candidates tested, the sequence PAT-11 bound to patulin with high affinity and excellent selectivity with a dissociation constant ( K d ) of 21.83 ± 5.022 nM. The selected aptamer, PAT-11, was subsequently used as a recognition element to develop a detection method for patulin based on an enzyme-chromogenic substrate system. The colorimetric aptasensor exhibited a linear range from 50 to 2500 pg mL −1 , and the limit of detection was found to be 48 pg mL −1 . The results indicated that GO-SELEX technology was appropriate for the screening of aptamers against small-molecule toxins, offering a promising application for aptamer-based biosensors.
研究领域:
毒理学
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分析化学
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食品科学
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PubMed
高影响因子
RSC Advances
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2015
·Vol. 6
·期 2
·1484-1490
·
IF 3.84
Hui-Fang Cui,Ya-Jun Li,Jia Wang,Xiao-Jia Li,Qiong-Lin Wang,Yan-Feng Bai
Pro-gastrin-releasing peptide (31–98) (ProGRP31–98) is a highly reliable, sensitive, and specific tumor marker for small cell lung cancer (SCLC). DNA aptamers for ProGRP31–98 were selected in this study from an 89-mer DNA library with a random region of 48 nucleotides (nt) flanked with two primer hybridization sites, by using the systematic evolution of ligands by the exponential enrichment (SELEX) method. The DNA sequences binding to ProGRP31–98 immobilized magnetic beads were selected by running 12 SELEX cycles and 3 negative selection cycles. The selected DNA sequences were separated by cell based DNA cloning, and then sequence analyzed. The DNA sequences obtained were screened and investigated for binding specificity to ProGRP31–98 by using electrochemiluminescence (ECL) measurement with [Ru(bpy)2dppz]2+, a molecular light-switch, as the ECL probe. One DNA sequence (89 nt) and its random region (48 nt) were identified as specific aptamers for ProGRP31–98. Based on the secondary structures of the obtained DNA aptamers, two other truncated DNA aptamers, 40 nt and 15 nt long, respectively, were identified. The obtained aptamers have strong affinities to ProGRP31–98, with a Kd value of 16 nM, and can detect ProGRP31–98 with a detection limit of 17 nM using the label-free ECL measurement.
研究领域:
分子生物学
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肿瘤学
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分析化学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Tingting Wang,Huanshun Yin,Yuting Zhang,Linkui Wang,Yue Du,Yuping Zhuge,Shiyun Ai
A simple and sensitive electrochemical method was developed for ampicillin detection based on the protective effect of aptamer-antibiotic conjugate towards endonuclease DpnII activity. Without ampicillin, DNA aptamer firstly hybridizes with the capture probe to form double strand DNA (dsDNA) structure. Then, dsDNA is cleaved by DpnII restriction endonuclease to form two dsDNA fragments. In which, one fragment is released from electrode surface and the other fragment is kept on electrode surface. Then, the dsDNA fragment kept on electrode surface is further digested by Exo III, which leads to the release of the dsDNA fragment from electrode surface. Thus, the electrochemical signal increases due to the decrease of the interface electron transfer resistance causing by the release of dsDNA from electrode surface. However, the formation of dsDNA is blocked when forming aptamer-ampicillin conjugate, which makes the obstruction of the digestion of DpnII and Exo III towards capture probe. Thus, a weak electrochemical signal is achieved due to the increase of the interface electron transfer resistance causing by the dsDNA on the electrode surface. Based on the relationship between ampicillin concentration and the decrease of the electrochemical signal, antibiotic is detected with low detection limit of 32 pM under optimal conditions, which is lower than the mandated maximum residue limit of European Union (9.93 nM). The developed method also presents good detection selectivity . Moreover, the applicability is confirmed by detecting antibiotic in milk and water samples with satisfactory results.
研究领域:
分析化学
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生物化学
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分子生物学
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PubMed
高影响因子
ANALYTICAL SCIENCES
·
2024
·
IF 1.8
Zhang Xuxin,Li Yihao,Xia Shaojie,Yang Zhenyuan,Zhang Baiyun,Wang Yonghong
Kanamycin (KAN) is widely used as a growth hormone analog and an antibacterial agent. However, abuse of this substance has resulted in the accumulation of excessive residue levels in foods of animal origin, which presents a significant risk to human health. A chemiluminescent aptasensor was constructed for the rapid quantitative detection of KAN by combining the properties of Co 3 O 4 nanoparticles (Co 3 O 4 NPs) nanozyme activity and DNA aptamer with high specificity. The DNA aptamer/Co 3 O 4 NPs nanozyme regulated the chemiluminescence signal by exploiting the chemiluminescent properties of luminol oxidation by H 2 O 2 . Specific binding of KAN to the aptamer led to the formation of a steric hindrance block in the solution, which inhibited the activity of nanozyme and reduced signal intensity. The degree of signal reduction is related to the concentration of KAN. Under optimal conditions, there was good linearity between KAN concentration and chemiluminescence signal intensity in the range of 0.5–8.0 μΜ, with a detection limit of 0.26 μΜ. The detection system performed well in the presence of competing antibiotics and was virtually unaffected. The method was also suitable for the detection of KAN in milk samples with sample recoveries of 97.8%–99.1%. The chemiluminescence sensor has the advantages of low cost, specificity, and sensitivity, and does not require an external light source or modification of the nucleic acid aptamer which makes it a promising candidate for applications in the field of food detection. Graphical abstract Schematic diagram of the chemiluminescence for KAN detection based on Co 3 O 4 NPs/KAN-apt.
研究领域:
分析化学
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食品科学
·
生物传感技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Chen Yiting,Gong Chunhui,Chen Kaiwei,Wang Ziwei,He Manli,Wang Peng,Chen Kai,Jiao Yan,Yang Yi
Strontium-90 ( 90 Sr) is a major radioactive component that has attracted great attention, but its detection remains challenging since there are no specific energy rays indicative of its presence. Herein, a biosensor that is capable of rapidly detecting Sr 2+ ions is demonstrated. Simple colorimetric method for sensitive detection of Sr 2+ with the help of single-stranded DNA was developed by preparing MnO 2 nanorods as oxidase mimic catalysis 3,3′,5,5′-tetramethylbenzidine (TMB). Under weakly acidic conditions, MnO 2 exhibited a strong oxidase-mimicking activity to oxidize colorless TMB into blue oxidation products (oxTMB) with discernible absorbance signals. Nevertheless, the introduction of a guanine-rich DNA aptamer inhibited MnO 2 -mediated TMB oxidation and reduced oxTMB formation, resulting in blue fading and diminished absorbance. Upon the addition of strontium ions to the system, the aptamers formed a stable G-quadruplex structure with strontium ions, thereby restoring the oxidase-mimicking activity of MnO 2 . Under the best experimental conditions, the absorbance exhibits a linear relationship with the Sr 2+ concentration within the range 0.01–200 μM, with a limit of detection of 0.0028 µM. When the concentration of Sr 2+ from 10 −8 to 10 −6 mol L −1 , a distinct color change gradient could be observed in paper-based sensor. We successfully applied this approach to determine Sr 2+ in natural water samples, obtaining recoveries ranging from 97.6 to 103% with a relative standard deviation of less than 5%. By providing technical solutions for detection, our work contributed to the effective monitoring of transportation of radioactive Sr in the environment. Graphical abstract
研究领域:
生物化学
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环境科学
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分析化学
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DOI
PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 6
·1986-1995
·
IF 4.616
Yu Luo,Ziying Jin,Jine Wang,Pi Ding,Renjun Pei
Aptamers, which are called chemical antibodies for their high affinity and specificity to targets, have great potential as analytical tools to detect pesticides. In this work, a DNA aptamer for thiamethoxam was isolated by an improved SELEX (systematic evolution of ligands by exponential enrichment) strategy, in which the ssDNA library was fixed on streptavidin-agarose beads through a short biotin labeled complementary strand. After 13 rounds of selection, the random ssDNA pool was successfully enriched. Three sequences were chosen as aptamer candidates through sequencing and analysis and were transformed into fluorescent probes to evaluate their interactions with thiamethoxam. A fluorescent turn-on aptasensor for thiamethoxam based on the best aptamer (FAM-Thi13) and a short quenching strand were further designed and showed a quantitative linear range from 10 to 1000 nM with a detection limit of 1.23 nM for thiamethoxam. Molecular docking and molecular dynamics were used to investigate the binding site of the main probe of the aptasensor (FAM-Thi13) and thiamethoxam. Satisfactory results were also obtained in quantifying thiamethoxam in environmental water samples by the developed fluorescent aptasensor.
研究领域:
分析化学
·
环境科学
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分子生物学
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DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2019
·Vol. 230
·期 6
·1-9
·
IF 1.774
Pu Hongbin,Huang Zhibin,Sun Da-Wen,Xie Xiaohui,Zhou Weibiao
Pollution of environmental endocrine disruptors has caused increasing concern globally. In the current study, a simple colorimetric method with high sensitivity and good selectivity for 17β-estradiol (E2) detection was developed, which employed gold nanoparticles (AuNPs) as colorimetric probe for specific recognition of shortening DNA aptamer. Visible color change from bright red to violet was observed for aggregation of AuNPs without the protection of DNA aptamer. After optimization, the method exhibited great performance for E2 detection with good linearity between E2 concentrations from 0.2 to 5 nM and the absorbance ratio at 620 and 523 nm, with the limit of detection of 0.1 nM. The method was also successfully applied to E2 determination in different spiked water samples including fishpond water, lake water, and tap water, in which good recoveries from 93.1 to 108.9% and acceptable relative standard deviations from 3.4 to 8.9% were obtained. This technique showed great potential for on-site fast determination of E2 in environmental water samples.
研究领域:
环境科学
·
分析化学
·
纳米技术
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产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Baozhu Zhang,Chunying Wei
A novel turn-on fluorescent biosensor has been constructed using C-PS2.M-DNA-templated silver nanoclusters (Ag NCs) with an average diameter of about 1 nm. The proposed approach presents a low-toxic, simple, sensitive, and selective detection for Pb 2+ . The fluorescence intensity of C-PS2.M-DNA-Ag NCs enhances significantly in the presence of Pb 2+ , which is attributed to the special interaction between Pb 2+ and its aptamer DNA PS2.M. Pb 2+ induces the aptamer to form G-quadruplex and makes two darkish DNA/Ag NCs located at the 3′ and 5′ terminus close, resulting in the fluorescence light-up. Moreover, Pb 2+ can be detected as low as 3.0 nM within a good linear range from 5 to 50 nM ( R = 0.9862). Furthermore, the application for detection of Pb 2+ in real water samples further demonstrates the reliability of the sensor. Thus, this sensor system shows a potential application for monitoring Pb 2+ in environmental samples.
研究领域:
生物化学
·
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Gu Shi,Chong Yan,Junhua Chen
Sensitive and reliable detection of antibiotics is of great significance for environmental and food safety due to its high risk in trace concentrations. Herein, we developed a fluorescence sensing system for chloramphenicol (CAP) detection based on dumbbell DNA-mediated signal amplification . Two hairpin dimers (2H1 and 2H2) were employed as the building blocks to construct the sensing scaffolds. The CAP-aptamer binding in another hairpin H0 can liberate the trigger DNA , which then activates the cyclic assembly reaction between 2H1 and 2H2. The separation of FAM and BHQ in the formed product of cascaded DNA ladder yields a high fluorescence signal for CAP monitoring. Compared with the monomer hairpin assembly between H1 and H2, the dimer hairpin assembly between 2H1 and 2H2 exhibits enhanced signal amplification efficiency and reduced reaction time. The developed CAP sensor showed a wide linear range from 10 fM to 10 nM with a detection limit of 2 fM. Importantly, this sensing platform has been successfully applied to the determination of CAP in fish, milk, and water samples with satisfactory recovery and accuracy. With the advantages of high sensitivity, mix-and-read pattern, and robustness, our proposed CAP sensor can be used as a simple and routine tool for the detection of trace amounts of antibiotic residues.
研究领域:
生物化学
·
食品安全
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Qiu Yan Zhu,Fu Rui Zhang,Yan Du,Xin Xing Zhang,Jiao Yang Lu,Qing Feng Yao,Wei Tao Huang,Xue Zhi Ding,Li Qiu Xia
Inspired by information processing and communication of life based on complex molecular interactions, some artificial (bio)chemical systems have been developed for applications in molecular information processing or chemo/biosensing and imaging. However, little attention has been paid to simultaneously and comprehensively utilize the information computing, encoding, and molecular recognition capabilities of molecular-level systems (such as DNA-based systems) for multifunctional applications. Herein, a graphene-based steganographically aptasensing system was constructed for multifunctional application, which relies on specific molecular recognition and information encoding abilities of DNA aptamers (Aeromonas hydrophila and Edwardsiella tarda-binding aptamers as models) and the selective adsorption and fluorescence quenching capacities of graphene oxide (GO). Although graphene–DNA systems have been widely used in biosensors and diagnostics, our proposed graphene-based aptasensing system can not only be utilized for fluorescence sensing and in vivo imaging of fish pathogens (A. hydrophila and E. tarda), but can also function as a molecular-level logic computing system where the combination of matters (specific molecules or materials) as inputs produces the resulting product (matter level) or fluorescence (energy level) changes as two outputs. More importantly and interestingly, our graphene-based steganographically aptasensing system can also serve as a generally doubly cryptographic and steganographic system for sending different secret messages by using pathogen-binding DNA aptamers as information carriers, GO as a cover, and a pair of keys, that is, target pathogen as a public key, the encryption key used to encode or decode a message in DNA as a private key. Our study not only provides a novel nanobiosensing assay for rapid and effective sensing and in vivo imaging of fish pathogens, but also demonstrates a prototype of (bio)molecular steganography as an important and interesting extension direction of molecular information technology, which is helpful in probably promoting the development of multifunctional molecular-level devices or machines.
研究领域:
生物传感
·
分子信息学
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）