ACS Applied Nano Materials
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2025
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IF 5.5
Yanci Zhang,Yujin Fan,Jiyuan Zhang,Yanxi Hu,Lili Duan,Zhanhui Wang,Yihong Wang,Jiansheng Cui,Liang Tian
The imbalanced expression of microRNA is related to many diseases and tumors, which can be used as a noninvasive biomarker to assist in the diagnosis, prognosis, and monitoring of cancer. A target conversion strategy-based electrochemical biosensor was developed by DNAzyme-cleavage dual cyclic signal amplification and the strand displacement reaction (SDR) to produce high electrochemical signals for microRNA-155 (miRNA-155) analysis. Thereinto, VSe2/multi-walled carbon nanotubes (MWCNTs) with high conductivity were applied as the electrode surface material to provide more active sites and accelerate electron transportation. Then, the excellent immobilization efficiency and large specific surface area of the ZnO@CuS nanoprobes were applied as a recognition molecule carrier. Furthermore, hexaammineruthenium(III) chloride (RuHex), with superior electrochemical redox activity, acted as the electroactive indicator by intercalating into double-helix DNA through electrostatic interaction, which produced high current responses in the presence of the target. Therefore, this miRNA-155 biosensing platform exhibited a relatively low limit of detection of 0.11 fM, ranging from 0.5 fM to 5 nM, and demonstrated excellent repeatability and stability. Hence, this platform appeared to possess favorable human serum detection capacity and be suitable for early cancer diagnostics.
研究领域:
生物医学工程
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分子诊断学
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纳米技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Huinan Chen,Jingyao Song,Yuanyuan Li,Dongmei Deng,Yuchen Song,Xiaoli Zhu,Liqiang Luo
Tumor biomarkers are a kind of important biomolecules for aiding cancer diagnosis and assessing tumor efficacy, among which alpha-fetoprotein (AFP) is a crucial tumor biomarker for the early diagnosis of hepatocellular carcinoma. In this study, a novel cascade signal amplifying strategy was designed for the ultrasensitive detection of AFP by DNAzyme cleaving-mediated hybridization chain reaction (HCR). The primary antibody was immobilized on the Au nanoparticles modified glassy carbon electrode, which subsequently captured AFP and the secondary antibody. Then, streptavidin was combined with the biotin-modified secondary antibody and Cu 2+ -specific DNAzyme. When Cu 2+ was present, the substrate strand of the DNAzyme would cleave and produce an ssDNA end. Finally, HCR was triggered to form long double-stranded DNA on the electrode, which would greatly inhibit the charge transfer at the electrode/solution interface. Therefore, the DNAzyme cleaving-mediated HCR strategy can be applied as cascade signal amplification for the ultrasensitive electrochemical determination of tumor biomarker AFP. Under optimal conditions, the immunosensor exhibits a broad linear range of 0.001−100 ng·mL −1 (R 2 = 0.990) and a low detection limit of 15.8 fg·mL −1 . The proposed sensor displays high sensitivity and selectivity for AFP detection, which can be employed as a universal sensing platform in clinical diagnostics.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Rui Zhang,Mingrui Zhang,Qiao Ding,Ruo Yuan,Yali Yuan
In this work, a novel label-free and signal-off electrochemical biosensor based on efficient target-trigger T-structure recycling with dual strand displacement amplification (T-DSDA) was constructed for sensitive detection of miRNA-155 that related to cardiovascular diseases. Compared with traditional SDA, the proposed strategy not only accomplished simple polymerization and shearing of dsDNA without continuous consumption of temple DNA , but also ingeniously designed two analogous sequences that produced double cleavage sites, thereby obtaining extensive output DNA for further cleaving Y-structure assembled on electrode with significantly improved target conversion efficiency. Since the redox probe methylene blue (MB) attach to Y-structure via electrostatic interaction and tessellation, an obviously decreased current with a typical signal-off mode could be acquired for sensing miRNA-155 in the assistant of DNAzyme cleavage recycling. As a result, the electrochemical biosensor realized the rapid and sensitive detection of miRNA-155 with a low detection limit down to 166.7 fM. In addition, the biosensor displayed a strong anti-interference ability in bovine whole blood, and thus was expected to be applied to detect blood samples of various miRNA-155 related diseases in clinical diagnosis.
研究领域:
生物化学
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分析化学
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生物医学工程
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Yinbo Ban,Fu Zhou,Hao Wang,Fuqiang Zhang,Mengmeng Xia,Yifei Wan,Suwan Yang,Rong Liu,Xiayan Wang,Guangfeng Wang
Endowing current artificial chemical reactions (ACRs) with high specificity and intricate activation capabilities is crucial for expanding their applications in accurate bioimaging within living cells. However, most of the reported ACR-based evaluations relied on either single biomarker stimuli or dual activators without obvious biological relevance, still limiting their accuracy and fidelity. Herein, taking the metal-ion-dependent DNAzyme cleavage reaction as a model ACR, two regulators, glutathione (GSH) and telomerase (TE) activated DNAzyme cleavage reactions, were exploited for precise discrimination of cancerous cells from normal cells. DNA probe was self-assembled into the ZIF-90 nanoparticle framework to construct coordination-driven nanoprobes. This approach enhances the stability and specificity of tumor imaging by utilizing biomarkers associated with rapid tumor proliferation and those commonly overexpressed in tumors. In conclusion, the research not only paves the way for new perspectives in cell biology and pathology studies but also lays a solid foundation for the advancement of biomedical imaging and disease diagnostic technologies.
研究领域:
生物医学工程
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分子生物学
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癌症研究
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纳米技术
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细胞生物学
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疾病诊断
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）