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CHEMISTRY-A EUROPEAN JOURNAL · 2014 ·Vol. 20 ·期 49 ·16065-16069 · IF 5.696

Simultaneous Detection of Multiple DNA Targets by Integrating Dual-Color Graphene Quantum Dot Nanoprobes and Carbon Nanotubes

Zhaosheng Qian,Xiaoyue Shan,Lujing Chai,Jianrong Chen,Hui Feng

Graphical Sensors : Simultaneous detection of multiple DNA targets was achieved with biocompatible graphene quantum dots (GQDs) and carbon nanotubes (CNTs) through spontaneous assembly of dual-color GQD-based probes and CNTs and subsequently self-recognition between DNA probes and targets (see scheme). Simultaneous detection of multiple DNA targets was achieved based on a biocompatible graphene quantum dots (GQDs) and carbon nanotubes (CNTs) platform through spontaneous assembly between dual-color GQD-based probes and CNTs and subsequently self-recognition between DNA probes and targets.

研究领域: 生物纳米技术 · 分子诊断 · 纳米材料 · 生物医学工程

✓ 已验证 DOI PubMed 高影响因子

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ANALYTICAL CHEMISTRY · 2021 · IF 6.986

Versatile CRISPR-Cas12a-Based Biosensing Platform Modulated with Programmable Entropy-Driven Dynamic DNA Networks

Chunyan Wang,Cuiyan Han,Xiaoxue Du,Weiwei Guo

In addition to their roles as revolutionary genome engineering tools, CRISPR-Cas systems are also highly promising candidates in the construction of biosensing systems and diagnostic devices, which have attracted significant attention recently. However, the CRISPR-Cas system cannot be directly applied in the sensing of non-nucleic acid targets, and the needs of synthesizing and storing different vulnerable guide RNA for different targets also increase the application and storage costs of relevant biosensing systems, and therefore restrict their widespread applications. To tackle these barriers, in this work, a versatile CRISPR-Cas12a-based biosensing platform was developed through the introduction of an enzyme-free and robust DNA reaction network, the entropy-driven dynamic DNA network. By programming the sequences of the system, the entropy-driven catalysis-based dynamic DNA network can respond to different types of targets, such as nucleic acids or proteins, and then activate the CRISPR-Cas12a to generate amplified signals. As a proof of concept, both nucleic acid targets (a DNA target with random sequence, T, and an RNA target, microRNA-21 (miR-21)) and a non-nucleic acid target (a protein target, thrombin) were chosen as model analytes to address the feasibility of the designed sensing platform, with detection limits at the pM level for the nucleic acid analytes (7.4 pM for the DNA target T and 25.5 pM for miR-21) and 0.4 nM for thrombin. In addition, the detection of miR-21 or thrombin in human serum samples further demonstrated the applicability of the proposed biosensing platform in real sample analysis.

研究领域: 生物技术 · 分子生物学 · 分析化学 · 诊断设备

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Simultaneous Detection of Multiple DNA Targets by Integrating Dual-Color Graphene Quantum Dot Nanoprobes and Carbon Nanotubes

Versatile CRISPR-Cas12a-Based Biosensing Platform Modulated with Programmable Entropy-Driven Dynamic DNA Networks