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Colorimetric assay of l-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters

Liang-Liang Wu,Li-Ying Wang,Zheng-Jun Xie,Na Pan,Chi-Fang Peng

This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l -cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for l -cysteine was developed with a detection limit (LOD) of 2.0   nM and a linear range from 5.0   nM to 500   nM. This detection for l -cysteine was highly selectivity over other amino acids . The high sensitivity and selectivity of this method facilitated the detection of l -cysteine in biological samples. Moreover, this method developed here was low-cost and simple. These results indicated the excellent analytical application of Ag/Pt NCs as enzyme mimic .