JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Qianji Li,Zejia Hao,Chenchen Zhang,Shanhong Ni,Ping Jiang,Pengfei Fan,Lei Li
In this article, a dual-signal sensor for the fluorescence and colorimetric detection of glyphosate (Gly) is developed based on DNAzyme-mediated click chemistry and DNAzyme-regulated CeO2 peroxidase-like activity. DNAzyme can bind to Cu+, triggering a click chemistry reaction between 3-Azido-7-hydroxycoumarin (AHC) and 3-ethyn-1-ol (BOL), thus generating a strong fluorescence signal at 475 nm. Due to the strong coordination between Gly and Cu2+, the amount of reduced Cu+ decreases, resulting in a weakening of the fluorescence. In addition, Gly can inhibit the catalytic site of CeO2 enzyme activity, while DNAzyme, which does not participate in the click chemistry reaction, can be adsorbed by CeO2, further inhibiting the enzyme activity and reducing the oxidation color change of 3,3′,5,5′-tetramethylbenzidine (TMB). The fluorescence detection limit of this dual-mode sensing platform is 0.15 μg/mL, and the colorimetric detection limit is 0.19 μg/mL. This method has been successfully applied to the detection of Gly in tap water and soybeans, which has a promising application in pesticide residue detection.
研究领域:
分析化学
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生物传感器
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环境科学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Mingdi Xu,Zhuangqiang Gao,Qiaohua Wei,Guonan Chen,Dianping Tang
A new signal-amplification strategy based on copper(II) (Cu 2+ )-dependent DNAzyme was developed for sensitive impedimetric biosensing of Cu 2+ in aqueous solution by coupling with target-induced formation of hemin/G-quadruplex-based DNAzyme and enzymatic catalytic precipitation technique. Initially, the target analyte cleaved the Cu 2+ -specific DNAzyme to generate an initiator strand on the sensor. Thereafter, the initiator strand underwent an unbiased strand-displacement reaction between hairpin probes in turn to construct a nicked double-helix, accompanying the formation of hemin/G-quadruplex DNAzyme on the long duplex DNA . The newly formed DNAzyme could oxidize the 4-chloro-1-naphthol (4-CN) to produce an insoluble precipitation on the sensor, thus resulting in a local alteration of the conductivity. Under the optimal conditions, the resistance increased with the increasing Cu 2+ in the sample, and exhibited a wide dynamic working range from 0.1 pM to 5.0 nM with a detection limit of 60 fM. The methodology also displayed a high selectivity for Cu 2+ relative to other potentially interfering ions owing to the highly specific Cu 2+ -dependent DNAzyme, and was applicable for monitoring Cu 2+ in real river samples. Thus, our strategy has a good potential in the environment surveys.
研究领域:
生物化学
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分析化学
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环境科学
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DOI
PubMed
高影响因子
Chemosensors
·
2025
·Vol. 13
·期 4
·123
·
IF 3.7
Yanfei Zhang,Yiling Zhang,Runqi Ouyang,Zong Dai,Si-Yang Liu
MircroRNA (miRNA) exhibits abnormal expression in many cancer diseases, and the detection and analysis of miRNA are significant for the early diagnosis of diseases and research on miRNA functions. In this work, we construct a UV-triggered DNAzyme (UTD) nanosensor for the early detection of miRNA in tumor cells. As the nanodevice was delivered into cells and irradiated by UV light, the controllable imaging of miRNA in living cells was achieved. This method effectively avoids false signal issues, providing a new strategy for high-spatiotemporal-resolution imaging of miRNA in living cells.
研究领域:
分子生物学
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医学诊断
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生物技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Jiuhong Zhao,Wenjing Zhu,Yuxuan Mao,Guixia Ling,Peng Zhang
The increased uric acid (UA) level is closely related to many diseases. However, the detection of UA remains great challenges due to the pain of blood testing and the complexity of common methods. Therefore, the detection of UA is of great significance for public health. In this paper, a new method for UA detection is proposed. The swellable hydrogel microneedles (hydrogel MNs) can be used for minimally invasive extraction of skin interstitial fluid (ISF). The colorimetric detection of trace UA in ISF can be successfully achieved by virtue of DNAzyme@MOFs with enhanced peroxidase activity. This study provides a powerful tool for minimally invasive, painless, rapid and convenient detection of UA in clinical applications. This study not only avoids the use of expensive and unstable natural enzymes but also expands the applications of DNAzyme@MOFs in biomedical detection.
研究领域:
医学检测
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生物化学
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材料科学
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DOI
高影响因子
Food Science & Nutrition
·
2021
·Vol. 9
·期 7
·3873-3884
·
IF 2.863
Mingcan Qin,Xiaoyi Ma,Shihui Fan,Hangjie Wu,Wanli Yan,Xiaopeng Tian,Jing Lu,Mingsheng Lyu,Shujun Wang
In the present study, a DNAzyme was screened in vitro through the use of a DNA library and crude extracellular mixture (CEM) of Pseudomonas aeruginosa . Following eight rounds of selection, a DNAzyme termed PAE-1 was obtained, which displayed high rates of cleavage with strong specificity. A fluorescent biosensor was designed for the detection of P. aeruginosa in combination with the DNAzyme. A detection limit as low as 1.2 cfu/ml was observed. Using proteases and filtration, it was determined that the target was a protein with a molecular weight of 10 kDa–50 kDa. The DNAzyme was combined with a polystyrene board to construct a simple indicator plate sensor which produced a color that identified the target within 10 min. The results were reliable when tap water and food samples were tested. The present study provides a novel experimental strategy for the development of sensors based on a DNAzyme to rapidly detect P. aeruginosa in the field.
研究领域:
分子生物学
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微生物学
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生物传感技术
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Xiaojiao Dai,Ziling Huang,Chuanyi Liu,Zhigang Li,Qingmin Chen,Yi Xu,Xiaomei Chen,Quansheng Chen,Jie Wei
Adenosine triphosphate (ATP) levels are recognized as a critical indicator of microbial content in food safety. The development of highly efficient ATP-sensing methods is essential for assessing food freshness. Herein, we propose a simple-yet-efficient hemin/G-quadruplex (G4) DNAzyme-based aptasensor for ATP detection and oyster freshness assessment. The anti-ATP aptamer subunits specifically bind to ATP, leading to self-assembly of the hemin/G4 DNAzyme. The newly produced DNAzyme then catalyzes the oxidation of ABTS 2- to produce the colored ABTS •− , providing an absorbance response for sensitive ATP analysis. The aptasensor facilitates high-performance and accurate determination of ATP with a low detection limit of 0.065 μM. Furthermore, this sensor enables reliable ATP analysis in oyster samples and evaluation of oyster freshness. In particular, a good linear correlation was established between microbial content and ATP levels. The proposed aptasensor for the assessment of freshness is characterized by its simplicity and efficiency, potentially offering a new tool to monitor food safety.
研究领域:
生物化学
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食品安全
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分子生物学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-9
·
IF 6.232
Wu Weihao,Fan Yaofang,Tan Bing,Zhao Huimin
With the merits of low cost, simple synthesis procedure, and high affinity for metal ions, deoxyribozyme (DNAzyme) have played important roles in metal ions detection. However, the intracellular applications of DNAzyme are limited because of enzymatic degradation and inefficient cellular uptake. To address these problems, GR-5 as model DNAzyme was encapsulated into zeolitic imidazolate frameworks-8 (ZIF-8) nanoparticles by biomimetic mineralization. The positively charged ZIF-8 with high DNAzyme loading capacity retained their ability to enter cells. Compared with free DNAzyme, the biomimetic mineralization synthesis method has greatly improved the stability of pristine DNAzyme. The as-synthesized DNAzyme@ZIF-8 composite exhibited good stability resisting DNase I, and was used as a sensitive fluorescent nanoprobe for Pb 2+ determination and successfully achieved selective and sensitive determination for Pb 2+ at λ ex / λ em = 494/522 nm in real samples. The linear range for the determination of Pb 2+ is 50 to 500 nM. Moreover, the highly active DNAzyme delivered by ZIF-8 allows noninvasive imaging of Pb 2+ measurement in living cells. This strategy will extend the suitability of functional nucleic acids for in vitro and in vivo bioanalysis and bioimaging.
Graphical abstract
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Cheng Qin,Xinlan Bian,Wei Lv,Bingzhi Li
CRISPR/Cas12a-based biosensors typically output fluorescent, colorimetric, and electrochemical signals, relying on reporters that suffer from low sensitivity, high cost, and low stability. Herein, we introduced a label-free chemiluminescent (CL) reporting system based on a DNAzyme known as “Supernova”. Supernova catalyzes the translocation of phosphate from a 1,2-dioxetane substrate (specifically, CDP-Star) to its 5′-OH ends, thereby triggering a CL reaction that produces light. Upon activation by the target, Cas12a cleaves Supernova, rendering it inactive in reacting with CDP-Star. Without pre-amplification, this biosensor can detect synthetic DNA corresponding to the SARS-CoV-2 N gene and monkeypox virus (MPXV) F3L gene with detection limits (LOD) of 1.5 and 2.4 pM, respectively. Integration with pre-amplification further enables the detection of SARS-CoV-2 and MPXV genes down to 1 copy/μL in real samples. Furthermore, the application of this CL system was expanded to the detection of non-nucleic acids with the integration of ATP aptamers, resulting in improved sensitivity in ATP biosensing compared to fluorescent assays. Overall, this work represents the first development of a label-free CL Cas12a reporting system, demonstrating its enhanced performance and broad applicability in synthetic biology-enabled sensing technology.
研究领域:
生物传感技术
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分子诊断学
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合成生物学
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DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 7
·1003-1011
·
IF 2.7
Yongwei Fang,Chuang Wang,Yuli Wang,Jiandong Hu,Miao Lai,Pengfei Liu,Hong Zhang
Plant viral diseases can seriously affect the yield and quality of crops. In this work, a convenient and highly sensitive biosensor for the visual detection of plant viral disease is proposed by the PCR-induced generation of DNAzyme. In the absence of nucleic acid for a target plant virus, the primers prohibited the production of G-quadruplex by forming a hairpin structure. However, PCR amplification occurred and generated a number of specific PCR products with free G-quadruplex sequences at both ends in the presence of the target cDNA. A catalytically active G-quadruplex DNAzyme was formed with the help of K+ and hemin, resulting in the formation of colored products visible to the naked eye and a strong absorbance by the addition of ABTS2− and H2O2. The absorbance and the logarithm of target cDNA concentrations showed a good linear relationship in the range of 10 fM–1.0 nM, with a linear regression equation of A = 0.1402 lg c + 0.3761 (c: fM) and a detection limit of 0.19 fM. This method was successfully applied to the analysis of emerging tobacco mosaic virus (TMV) infections in tobacco leaf samples collected in the field due to its flexibility and convenience, indicating a potential application for the early detection of plant viral disease.
研究领域:
植物病理学
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分子生物学
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DOI
PubMed
高影响因子
ACS Nano
·
2019
·
IF 13.903
Jing Wang,Huimin Wang,Hong Wang,Shizhen He,Ruomeng Li,Zhao Deng,Xiaoqing Liu,Fuan Wang
The development of self-assembled DNA nanomedicine requires a facile and accurate DNA degradation strategy for precisely programmable drug release. Conventional DNA catabolic strategies are restrained with the fragile and unclear enzymatic reactions that might lead to inefficient and uncontrollable digestion of DNA scaffolds and thus might bring undesirable side effects to the sophisticated biosystems. Herein we reported a versatile self-sufficient DNAzyme-driven drug delivery system consisting of the rolling circle polymerized DNAzyme–substrate scaffolds and the encapsulated pH-responsive ZnO nanoparticles (NPs). The full DNAzyme nanosponges (NSs) were also encoded with multivalent tandem aptamer sequences to facilitate their efficient delivery into cancer cells, where the acidic endo/lysosomal microenvironment stimulates the dissolution of ZnO into Zn2+ ions as DNAzyme cofactors and therapeutic reactive oxygen species generators. The supplement Zn2+ cofactors mediated the nonviolent DNAzyme-catalyzed cleavage of DNA scaffolds for precise and efficient drug administrations with synergistically enhanced therapeutic performance. The facile design of DNAzyme, together with their cost-effective and intrinsic robust features, is anticipated to provide extensive insights for the development of DNA-based therapeutic platforms by activating the specific intracellular biocatalytic reactions. As an intelligent and nonviolent self-driven drug delivery platform, the present DNAzyme NS system could be engineered with more therapeutic sequences and agents and was anticipated to show exceptional promise and versatility for applications in biomedicine and bioengineering.
研究领域:
纳米技术
·
药物递送系统
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癌症治疗
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分子生物学
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生物医学工程
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jiuhong Zhao,Jinnan Zhao,Xiaoyu Zhang,Guixia Ling,Peng Zhang
The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the in vivo DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.
研究领域:
生物化学
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医学检测
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纳米材料科学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 110
·108662-108667
·
IF 3.289
Jin-Xiu Cao,Yong-Sheng Wang,Jin-Hua Xue,Yan-Qin Huang,Ming-Hui Li,Si-Han Chen,Bin Zhou,Xian Tang,Xiao-Feng Wang,Yu-Feng Zhu
We report on a novel and highly sensitive strategy for UO22+ detection. The designed single-labeled hairpin signal probe (SSP) was employed as a reporter, and a multipurpose RNA-cleaving DNAzyme as a target recognition element, catalytic DNAzyme and the primer of substrate fragment recycling amplification. The presence of UO22+ results in the substrate strand in DNAzyme being cleaved at the RNA site, releasing short substrate fragments. The fragment that is complementary partially with SSP could open the hairpin of SSP to form a double-stranded DNA with recessed 3′-termini, which could be selectively digested by exonuclease III. After the double-stranded DNA is fully consumed, the substrate fragment is released. This one could hybridize with another SSP to initiate the next round of digestion by exonuclease III. The recycling of substrate fragments can be repeated multiple times, resulting in the accumulation of the more 6-carboxyfluorescein in the solution along with an amplified fluorescence signal. By this strategy, we achieved hitherto an unreported UO22+ detection limit of 2.4 pM. Therefore, this novel exonuclease III-amplified strategy has great potential for highly sensitive and selective quantification of UO22+ in biomedical research and environmental science fields.
研究领域:
生物化学
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环境科学
·
分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）