Advanced Healthcare Materials
·
2023
·Vol. 12
·期 21
·2203252
·
IF 10
Xuan Nie,Wei You,Ze Zhang,Fan Gao,Xiao-Hong Zhou,Hai-Li Wang,Long-Hai Wang,Guang Chen,Chang-Hui Wang,Chun-Yan Hong,Qi Shao,Fei Wang,Lei Xia,Yang Li,Ye-Zi You
Gene therapy holds great promise as an effective treatment for many diseases of genetic origin. Gene therapy works by employing cationic polymers, liposomes, and nanoparticles to condense DNA into polyplexes via electronic interactions. Then, a therapeutic gene is introduced into target cells, thereby restoring or changing cellular function. However, gene transfection efficiency remains low in vivo due to high protein binding, poor targeting ability, and substantial endosomal entrapment. Artificial sheaths containing PEG, anions, or zwitterions can be introduced onto the surface of gene carriers to prevent interaction with proteins; however, they reduce the cellular uptake efficacy, endosomal escape, targeting ability, thereby, lowering gene transfection. Here, it is reported that linking dipicolylamine-zinc (DPA-Zn) ions onto polyplex nanoparticles can produce a strong hydration water layer around the polyplex, mimicking the function of PEGylation to reduce protein binding while targeting cancer cells, augmenting cellular uptake and endosomal escape. The polyplexes with a strong hydration water layer on the surface can achieve a high gene transfection even in a 50% serum environment. This strategy provides a new solution for preventing protein adsorption while improving cellular uptake and endosomal escape.
研究领域:
生物医学工程
·
基因治疗
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Jun-Dan An,Tian-Tian Wang,Yang-Fan Shi,Jian-Zhong Huo,Xiang-Xia Wu,Yuan-Yuan Liu,Bin Ding
In recent years, a new type of micro-porous material, namely metal organic framework material, has received more and more attention from many basic and industrial fields because these materials possess unique advantages. In this work, through the powerful sonochemical preparation method, a three-dimensional cluster-based Cd II -MOFs, {[Cd(abtz) 2 (H 2 O) 2 ]·(ClO 4 ) 2 ·H 2 O} n ( 1 , abtz = 1-(4-aminobenzyl)-1H-1,2,4-triazole) can be quickly synthesized in the facile ultrasonic method. Powder X-ray diffraction (PXRD) measurement confirms that these bulky samples 1 (synthesized on different ultrasonic powers and ultrasonic time conditions) were pure . In addition, ultrasonic chemical time and irradiation power did not change the structure of composites materials 1 . SEM and morphological changes of 1 in the ultrasonic synthesis are also determined. Moreover, 1 exhibits good stability, the structure of 1 can be maintained not just in various solvents, and in aqueous environments with pH values from 2 to 12. Photo-luminescent experiment also reveals that complex 1 has the excellent application prospect as highly sensitive sensing material for the biomarker DPA (2,6-pyridine dicarboxylic acid) and 5-HT (5-hydroxytryptamine) through the photo-luminescence “turn-on” and “turn-off” effect, respectively. Further photo-luminescent measurements also show that different ultrasonic powers and ultrasonic time can effectively induce fluorescent sensing enhancement for biomarkers DPA and 5-HT based on the water stable clustered-based cadmium(II) coordination framework.
研究领域:
材料科学
·
生物学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Jie Mingsha,Zhu Amei,Zhu Bing,Guo Ruipeng,Lan Shengkai,He Jiaxin,Lang Xingang,Bai Yanhong
The overuse of antibiotics leads to significant health risks and environmental challenges. Therefore, selective and sensitive detection of antibiotic residues is a critical issue. Here, a double-emission ratiometric fluorescence probe (Lu-Eu-DPA) was constructed for the selective detection of gentamicin sulfate (GEN), neomycin (NEO), and erythromycin (EM). The Lu-Eu-DPA coordination polymer nanoparticles composed of luminol, the coordinated nuclear metal Eu 3+ , and 2,6-pyridinedicarboxylic acid (DPA). The coordination reaction between luminol molecules and Eu 3+ ions produce the aggregation-induced emission (AIE) effect, while DPA acts as a ligand for the antenna, absorbing energy from the excitation light and then transferring the energy to Eu 3+ . Based on the AIE effect and antenna effect (AE), the fluorescence intensity of Lu-Eu-DPA at 426 nm and 617 nm was correlated with the concentration of GEN, NEO, and EM, and the detection limits of GEN, NEO, and EM were 20, 25, and 48 nmol/L, respectively. The detection capability of the proposed method in pork samples was also studied. In addition, semi-quantitative determination of GEN, NEO, and EM was conveniently and efficiently performed using a smartphone-based visualization method. These results suggested that this probe with good selectivity and specificity was a promising method for swiftly detecting multiple antibiotic residues.
研究领域:
分析化学
·
食品安全
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Zhen-Peng Cui,Le-Xi Zhang,Heng Xu,Yan-Yan Yin,Bo Tang,Li-Jian Bie
Metal-organic frameworks (MOFs) have been proved to be outstanding candidates for humidity sensors, however, pure MOFs are still limited in hysteresis, integration, and intelligence. In this investigation, inspired by a single-site-ion strategy for catalysis, UiO-66-NH 2 is first grafted by 2, 6-DPA (2, 6-dipicolinic acid) molecules (named as U-DPA) and then combined by Zn (II) ions (noted as U-DPA-Zn). Taking advantage of electrolyte and MOF, U-DPA-Zn exhibits significantly enhanced humidity sensing performance than UiO-66-NH 2 and U-DPA. U-DPA-Zn possesses the highest sensitivity (S r = 8.07 × 10 3 ), the best linearity (R 2 = 0.994), the shortest response-recovery time (τ res = 2 s, τ rec = 105 s), the smallest hysteresis (ΔH = 1.81%), as well as good repeatability in a wide relative humidity (RH) range (11%-97%). Thanks to its excellent properties, U-DPA-Zn shows great potential in the field of monitoring human respiration (breath detection) and water evaporation on human skin (finger detection). Additionally, the humidity sensing mechanism is discussed based on complex impedance analysis, revealing the single Zn (II) make giant contribution to MOFs. This work provides an optional material for humidity sensors and further a new strategy to improve the humidity sensitivity of pure MOFs.
研究领域:
材料科学
·
传感技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 7
·1453-1465
·
IF 3.286
Niazi Sobia,Khan Imran Mahmood,Yan Lv,Khan Muhammad Issa,Mohsin Ali,Duan Nuo,Wu Shijia,Wang Zhouping
A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B 1 (FB 1 ) was developed using multi-color, Ln 3+ -doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF 4 : Ce, Tb and NH 2 -Eu/DPA@SiO 2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe 3 O 4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb 3+ and Eu 3+ , respectively, were used to measure FB 1 ( Y = 19,177.1 + (− 12,054.4) x , R 2 = 0.9917) and OTA ( Y = 4138.8 + (− 11,182.6) x , R 2 = 0.9924), respectively. The limits of detection (LODs) for FB 1 and OTA were 0.019 pg mL −1 and 0.015 pg mL −1 , respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB 1 while detection range varied from 0.0001–0.5 ng mL −1 . This aptasensor was effectively applied to quantity FB 1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.
Graphical abstract
研究领域:
食品安全
·
分析化学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 34
·50843
·
IF 3.125
Xuefei Shao,Junnan He,Xuemei Zhao,Yubin Zheng,Nan Zheng
Cationic polyesters have been widely utilized as efficient gene delivery carriers. Their ability in binding genes was majorly based on the electrostatic effect between the positive charges of polymers and the negatives charges of genes. It has been well known that large numbers of positive charges on the polymers would lead to undesired toxicity although strong gene binding capability. It was of great interest to developed a polymer with reduced positive charges while enhanced gene condensation ability. In this work, a library of polyesters functionalized by zinc-coordinated-dimethylpyridinium amine (DPA-Zn) have been successfully prepared by the polycondensation method starting from dimethyl 1,3-acetonedicarboxylate and 10 diols, followed by the post-modification using dimethylpyridinium amine and zinc nitrate. The post-modification efficiency was systemically evaluated and the optimized functionalization efficiency could reach around 50%. The gene condensation ability of the targeting polymers was also evaluated using gel retardation assay and dynamic light scatting. The results indicated that DPA-Zn functionalized polyesters could bind gene into nanocomplexes with the sizes around 200 nm.
研究领域:
生物材料学
·
基因递送
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 48
·期 4
·1642-1649
·
IF 3.3
Xurong Zuo,Pengfei Liu,Qinhao Sun,Jiahui Huang,Yuwei Zhang,Xuecheng Zhu,Runqi Chen,Xiangyu Meng
Because anthrax spores are highly lethal to humans and animals as well as potential biological warfare agents, there is a great need for rapid, sensitive, and selective quantification of dipicolinic acid (DPA) as a biomarker for anthrax spores. Herein, we prepared a novel lanthanide-doped nanoprobe by coordination of Tb3+ ions with tannic acid (TA)-coated zeolitic imidazolate frameworks-8 (ZIF-8) (ZIF-8@Tb–TA). The proposed assay was derived from the unsaturated coordination of Tb3+ ions. In the presence of DPA, Tb3+ ions could be sensitized to emit inherent luminescence due to the energy transfer after coordination with DPA. The results illustrated that ZIF-8@Tb–TA could be used as a sensitive nanoprobe for detecting DPA with a broad linear range (0 μM to 12.0 μM), and the limit of detection (LOD) was 12.3 nM. Moreover, the specific recognition ability of DPA by ZIF-8@Tb–TA was much better than that of other interfering molecules. This study proposed a new strategy to establish lanthanide-functionalized nanoprobes for highly selective and sensitive detection of DPA.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhizhou Liu,Tong Wang,Liang He,Xueyan Nan,Xuming Sun,Pengli Bai
Bacillus anthracis, an extremely dangerous bacterium which can cause pulmonary anthrax and be used as biochemical weapon agent, can be detected by the detection of its major biomarker 2, 6-pyridine dicarboxylic acid (DPA). Herein, we reported the solvothermal synthesis of a non-luminescent NH 2 -BDC-Eu-MOF using Eu 3+ and 2-aminoterephthalic acid (NH 2 -BDC). The emission of amino ligand and Eu 3+ ions are absent in ethanol while the addition of DPA could recover both emission at 460 and 624 nm respectively. Thus this MOF can act as a dual emission ‘‘turn-on’’ probe for DPA detection. The detection limits (LOD) of this DPA probe are about 0.8 μM in ethanol and 0.36 μM in water, which are much lower than an infectious dosage of bacillus anthracis spores (60 μM) for human being. In order to monitor DPA levels more conveniently, we developed a one-to-two decoder logic gate operation, which can determinate DPA via color discrimination.
研究领域:
生物化学
·
材料科学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
ChemistrySelect
·
2026
·Vol. 11
·期 36
·e74577
·
IF 2.3
Haixia Hu,Yiying Shi,Zhongyu Duan,Wenjun Zhang
Zirconium-based metal–organic framework (MOF) UiO-66 is a commonly used host framework material in the research field of fluorescent probes. However, the pure UiO-66 material lacks functional sites for targeted recognition and thus cannot achieve specific fluorescence detection. In view of this, terephthalic acid (TPA) and 2,6-pyridinedicarboxylic acid (DPA) were used as mixed ligands to coordinate with Zr 4+ for constructing a MOF in this paper. Subsequently, 4-methyl-7-hydroxycoumarin (4-Mu) and Eu 3+ were introduced for functional modification, and UiO-66(DPA)@4-Mu/Eu 3+ fluorescent material with dual emission centers was finally synthesized. It was investigated that UiO-66(DPA)@4-Mu/Eu 3+ can serve as a dual-emission ratiometric fluorescent probe for the efficient detection of doxycycline (DOX) content in aqueous solution. Under the excitation wavelength of 365 nm, the fluorescence color of the probe system gradually changed from blue to red with the increase of DOX concentration. Further analysis revealed that the fluorescence intensity ratio I 618 /I 430 of the system was positively correlated with DOX concentration, enabling highly sensitive detection of DOX with a limit of detection as low as 2.64 µM. Graphical Zr 4+ coordinates with terephthalic acid and 2,6-pyridinedicarboxylic acid as mixed ligands. 4-Methyl-7-hydroxycoumarin is introduced together with Eu 3+ to realize functionalization. The obtained UiO-66(DPA)@4-Mu/Eu 3+ material exhibits dual-emission property and can efficiently detect doxycycline in aqueous solution, which provides a new strategy for the functional design of metal–organic frameworks (MOFs).
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 33
·4267-4273
·
IF 2.378
Miao Xu,Wei Huang,Dingkun Lu,Chunyu Huang,Jingjing Deng,Tianshu Zhou
In this work, a novel dual-sensing probe was developed for detecting an anthrax biomarker (dipicolinic acid, DPA) based on the competitive coordination of Tb3+ between DPA and Alizarin Red (AR). UV-vis titration experiments demonstrated that AR and Tb3+ ions formed a 3 : 1 coordination pair in a boric acid–borate buffer solution. However, the subsequent addition of DPA to the complex solution destroyed the coordination of AR–Tb3+ completely, resulting in the formation of DPA–Tb3+. This constructed a new mechanism of ratiometric colorimetric and fluorescence dual-mode sensing for DPA. By integrating a smartphone with an easy-to-access color-scanning APP, this dual-sensing method could be further applied as a reliable scanometric assay for the rapid and on-site visible detection of DPA directly. With the method established by us, the sensitive and selective detection of DPA released from bacterial spores could be realized easily.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
iScience
·
2025
·
IF 4.1
Yingming Wang,Lingyan Xu,Lu Liang,Zhentao Qin,Zhuochen Cai,Kaifu Zhang,Chongqi Liu,Lixiang Lian,Wei Zheng,Yanyan Lei,Qinzeng Hu,Binbin Zhang,Tao Wang,Wanqi Jie
Organic scintillators occupy a significant niche in the realm of fast neutron detection. Nevertheless, the cultivation of large-sized and high-quality organic single crystals has persistently posed a formidable challenge. 9,10-diphenylanthracene (DPA) was chosen as a prospective material for fast neutron detection. The two-dimensional nucleation growth mechanism of DPA was revealed by detailed analysis of the microstructure of the crystal surface. By systematically optimising the growth parameters and processes, the nucleation and growth of DPA were effectively controlled, and centimetre-sized single crystal (27×20×7 mm 3 ) was successfully grown. The thermally activated fluorescence emission mechanism of DPA crystal was revealed using power-dependent and temperature-dependent photoluminescence spectroscopy. The fluorescence intensity increased with increasing temperature, suggesting its potential for neutron/gamma pulse shape screening (PSD) at high temperatures. High-quality crystal was prepared, achieving a FOM of 3.56, underscoring the potential of DPA as a superior material for fast neutron detection applications.
研究领域:
材料科学
·
核物理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 3
·1-11
·
IF 21.8
Lin Xiaodong,Cao Zhengyang,Zeng Shiyu,Zhu Haoyu,Zhai Kairui,Yin Binfeng,Zhang Cheng,Peng Tao,Cheng Teng,Zhang Biao
In this work, we developed a dual-mode portable device that integrated a 3D-printed microfluidic chip for detection of dipicolinic acid (DPA) on chip. The system uses a ratiometric fluorescence nanoprobe formed by embedding carbon dots (CDs) into an Eu 3 ⁺ metal–organic framework (Eu-MOF). Upon reaction with DPA in the microchannel, red fluorescence was enhanced and blue fluorescence suppressed, enabling sensitive ratiometric detection of DPA on chip with a detection limit (LOD) of 0.04 µM. Interestingly, the composite Eu MOF /CDs/DPA also exhibits peroxidase-like activity, catalyzing the oxidation of TMB into a blue-colored product (oxTMB), which allows for colorimetric detection with an LOD of 10.14 µM. To improve usability and reduce environmental or instrumental variability, incorporating a microfluidic chip into a semi-portable device and utilizing a smartphone, making the system portable and miniaturized for easy operation. In the smartphone-assisted mode, the LODs were 0.33 µM (ratiometric fluorescence) and 12.27 µM (colorimetry), determined by RGB signal analysis, respectively. Moreover, satisfactory recoveries (85–104.6%) were achieved in the spiked real samples. Overall, this platform offers a straightforward, cost-effective, and versatile approach for DPA detection, with promising applications in food safety, environmental monitoring, and clinical diagnostics.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）