CHEMOSPHERE
·
2019
·
IF 5.108
Jing Zou,Huahua Cai,Daiyao Wang,Junyang Xiao,Zhenming Zhou,Baoling Yuan
A low-cost and environmentally-friendly spectrophotometric method for hydrogen peroxide (H 2 O 2 ) determination based on the oxidative coloration reaction of N,N′ -diethyl- p -phenylenediamine (DPD) via the Fenton reactions in aqueous water was established. The generated pink radical cation (DPD + ) showed maximum absorption at 551 nm. Importantly, under the optimal conditions (pH 3.0, 20 mM DPD, 1.5 mM Fe(II) and reaction time of 45 s), the increase in absorbance at 551 nm for DPD + generation was linear with respect to the addition of H 2 O 2 (0–12 μM). The sensitivity and the detection limit of the proposed Fenton-DPD method for H 2 O 2 determination at 551 nm were (2.55 ± 0.01) × 10 4 M −1 cm −1 and 0.05 μM, respectively. The stoichiometric factor for the reaction of H 2 O 2 with DPD was 1:1.18. The absorbance of the generated DPD + was found to be stable in different types of water within 20 min. Moreover, the proposed Fenton-DPD method could be used for the analysis of the trace H 2 O 2 in rainwater and determine the rate constants that involved H 2 O 2 with high accuracy.
研究领域:
分析化学
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环境科学
·
生物化学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Tao Li,Lin Dai,Yixin Huang,Siwen Pan,Zijun Pang,Jing Zou
Nowadays, a number of spectrophotometric methods for Cr(VI) determination have been developed. Whereas most of them are sensitive to pH, metallic ions, temperature, etc, which render them out of feasibility and may result in erroneous measurement. Therefore, to overcome these drawbacks, a new spectrophotometric method with rapid detection, high accuracy and strong anti-interference ability was established. This method is based on the oxidative coloration of N,N -diethyl- p -phenylenediamine (DPD) and Cr(VI) to generate pink-colored DPD •+ radicals which showed maximum absorbance at 551 nm. Accordingly, different reaction parameters were optimized, the calibration curves (linear range 0.85–60 µM) were established with a high sensibility of (3.05 ± 0.02) × 10 4 M −1 cm −1 . The LOD and LOQ were determined to be 0.26 µM and 0.85 µM respectively, accompanied with a stoichiometric coefficient of near 1:3 (1:2.78). The DPD method exerted a satisfactory accuracy in natural water samples and a pretty good anti-interference performance towards various common co-existing metallic ions. Moreover, this method was successfully applied in the Cr(VI)-sulfite and Cr(VI)-hydroxylamine systems. Overall, the study highlights the DPD method as a promising approach for the Cr(VI) determination and provides a new perspective for the practices of spectrophotometry .
研究领域:
分析化学
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环境科学
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DOI
高影响因子
CHEMOSPHERE
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2020
·
IF 5.778
Zijun Pang,Yajuan Cai,Weihao Xiong,Junyang Xiao,Jing Zou
A new spectrophotometric method for measuring permanganate index (chemical oxygen demand using potassium permanganate (KMnO 4 ) as oxidant, COD Mn ) in water was established. The method was based on the rapid oxidation of N,N -diethyl- p -phenylenediamine (DPD) by residual KMnO 4 in digestion solution under neutral pH condition to form the stable pink radical (DPD ●+ ). Only 20 s were enough to form the pink DPD ●+ . The generated DPD ●+ could be quantitatively measured by a visible spectrophotometer at 551 nm. Stoichiometric coefficient of the reaction between KMnO 4 and DPD was close to 1:5 (1:5.07). There was a well linear relationship (R 2 = 0.999) between the change of the absorbance of DPD ●+ at 551 nm and the concentration of COD Mn in the range of 0–4.46 mg L −1 . Limit of detection of the DPD method was as low as 0.02 mg L −1 COD Mn . The DPD method was highly accurate for measuring COD Mn in standard solutions with well recovery rates of 99.17%–102.22%, and was well tolerant to the interference of coexistent Cl − and Fe 3+ . The DPD method was successfully applied for measuring COD Mn in real water samples, including surface water, underground water and drinking water . In comparison to the traditional titration method, the proposed DPD method was more convenient to operate, required less samples and digestion reagents (i.e., KMnO 4 and H 2 SO 4 ) and could be employed for online monitor.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2011
·Vol. 13
·期 38
·17323-17332
·
IF 3.454
Shu-le Lin,Xiu-fang Wen,Zhi-qi Cai,Pi-hui Pi,Da-feng Zheng,Jiang Cheng,Li-juan Zhang,Yu Qian,Zhuo-ru Yang
Developing microstructures, such as low molecular aggregates, spherical micelles and multi-compartment micelles, is an expanding area of research in Materials Science. By applying an atom transfer radical polymerization (ATRP) process to cross-linkable fluorinated diblock copolymers and analyzing the data we are able to demonstrate the potential for developing films with different micro-structures for additional biological research. Applying the Dissipative Particle Dynamic (DPD) Method, Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) techniques to cross-linkable fluorinated diblock copolymers of (methyl methacrylate-co-hydroxyethyl methacrylate-co-butyl methacrylate)-b-2-(perfluoroalkyl)ethyl methacrylate (MMA-co-HEMA-co-BMA-b-FMA) we were able to analyze the structures and their relationships to the aggregation of various microstructure formations through the use of various solvents in the process. For the self-assembly of the cross-linkable diblock copolymer in solutions, the DPD simulation results are only in qualitative agreement with experimental data of aggregate morphologies and sizes. This suggests an improved approach to creating materials and methods necessary for studying microstructures in films used in other research areas. Our work examines whether using selective solvents can be easily extended to prepare aggregates with different morphologies, which is an effective shortcut to obtain films with different microstructures. DPD simulation can be considered as an adjunct to experiments and provides other valuable information for the experiment.
研究领域:
材料科学
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高分子化学
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生物材料
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DOI
高影响因子
CHEMOSPHERE
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2018
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IF 4.427
Xin Liu,Huahua Cai,Jing Zou,Zijun Pang,Baoling Yuan,Zhenming Zhou,Qingfeng Cheng
A sensitive spectrophotometric method (the N,N -diethyl- p -phenylenediamine (DPD) method) was established for the determination of trace permanganate concentration (0–10 μM) in water. The DPD method was based on the oxidative coloration reaction where permanganate could oxidize DPD to form the red colored DPD radical (DPD • + ) with a second-order rate constant of 2.96 × 10 4 M −1 s −1 at pH 6 (50 mM phosphate buffer). The generated DPD • + could be quantitatively measured at 551 nm using an UV–Vis spectrophotometer. There was a good linear relationship (R 2 = 0.999) between the absorbance of DPD • + and permanganate concentration. The DPD method was highly sensitive, and the absorbance of generated DPD • + at 551 nm was as high as 5.70 × 10 4 cm −1 per M (mol L −1 ) of permanganate. The reaction of permanganate with DPD in the pH range of 4.0–8.0 had a stoichiometric coefficient of 1:2.71. The residual absorbance of DPD •+ in ultrapure water and natural waters was fairly stable for 30 min. Limits of detection of the proposed DPD method in ultrapure water and natural waters were calculated to be as low as 0.010 μM and 0.017 μM, respectively. Moreover, trace permanganate concentrations of 0.04 and 0.10 μM were found in natural waters and wastewater by the proposed DPD method. Additionally, the DPD method could be applied to measure the second-order rate constants of the reaction of permanganate with phenol at pH 7.0 (28.2 M −1 s −1 ).
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Yuanhui Tang,Mingxuan Li,Yuqian Yue,Huanhuan Wu,Zhijun Zhang,Chunhui Zhang,Lixin Yu,Haihui Wang,Qiang Zhang,Xiaolin Wang,Yakai Lin
The rapid and complex dynamics of interfacial polymerization (IP) have long hindered real-time monitoring, limiting fundamental insights into the synthesis–structure–performance relationship of polyamide thin-film composite (PA TFC) membranes. In this work, the IP process, where aqueous and organic monomers react to form a thin PA membrane layer, was successfully simulated using the Dissipative Particle Dynamics (DPD) method at a large scale, with the hexane-trimesoyl chloride/water-piperazine (hexane-TMC/water-PIP) system serving as a representative model. Coarse-grained models and interaction parameters for all molecular species were first developed, and a DPD-based protocol for simulating the IP reaction was subsequently established, enabling a systematic investigation of membrane formation at the mesoscale level. By adopting the concentrations employed in experimental studies, the simulation results showed that, in the early stage of IP, high-molecular-weight polymer chains form rapidly; as steric hindrance accumulates, shorter chains are increasingly generated, stabilizing the PA molecular weight. Then, the effect of monomer concentration on membrane formation dynamics and the resulting PA layer morphology was systematically investigated. The results showed that simultaneous increases in both monomer phases promote the formation of shorter PA chain segments, leading to increased membrane thickness and reduced pore size. In contrast, increasing the concentration of a single monomer phase favors the formation of longer PA chains, resulting in negligible changes in membrane thickness while slightly reducing pore size. Finally, a support-free IP process was employed to prepare PA membranes, and the corresponding membrane structures and thickness evolution were measured to validate the simulation results. This work aims to provide a large-scale, DPD-based model capable of simulating the IP process in different practical systems to form TFC membranes, thereby enabling a deeper understanding of membrane formation kinetics.
研究领域:
高分子科学
·
膜科学与技术
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化学工程
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材料科学
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计算化学
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分离科学
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产品关联
DOI
高影响因子
DRUG METABOLISM AND DISPOSITION
·
2026
·
IF 3.6
Qianqian Zhang,Jingyi Hu,Yangbo Liu,Dongzhao Xie,Linyue Bai,Zhuang Hu,Yanjun Tang,Siqi Feng
The therapeutic efficacy of 5-fluorouracil (5-FU), a cornerstone of gastric cancer chemotherapy, is predominantly limited by its catabolic inactivation in tumors. Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme responsible for 5-FU inactivation, and its tumor-specific overexpression constitutes a primary mechanism of 5-FU resistance. Here, we report a novel strategy to increase the sensitivity of 5-FU by targeting the post-translational regulation of DPD. We demonstrate that the NEDD8-activating enzyme (NAE) inhibitor MLN4924 significantly enhancesthe antitumor activity of 5-FU in both cellular and animal models of gastric cancer without augmenting systemic toxicity. Mechanistically, MLN4924 treatment inhibits the neddylation of DPD, which is dependent on the NAE1/UBC12 axis. This inhibition triggers the ubiquitination and subsequent proteasomal degradation of DPD, thereby reducing intracellular 5-FU catabolism and augmenting its cytotoxic effects. Our findings identify neddylation as a previously unrecognized regulatory mechanism governing DPD protein stability and activity. This work identifies the neddylation-DPD axis as a novel therapeutic target and provides a strong rationale for combining NAE inhibition with 5-FU-based chemotherapy in gastric cancer.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Yu Tian,Zhijie Zhu,Qing Li,Jing Zhang,Cai-Feng Wang,Guan Wu,Shen Shen Li,Ji Jun Xiao,Su Chen
Methods that allow the self-assembly of colloidal nano/micro-particles into controllable structures are highly important in many applications, such as photonics, biosensors and electronic chemicals. However, the complicated procedures, low assembly efficiency and multiscale integration of self-assembly of colloidal particles have become great challenges. Here, we first develop a hydrophobic-force-induced self-assembly (HFSA) method to fabricate homogeneous and binary colloidal microdroplets by the feed of volatile fluorocarbon oil. Specially, both mono-color and dual-color colloidal photonic crystals (CPCs) can be architected via HFSA in an efficient (within 200 s), coffee-ring free and visualized fashion. Additionally, such effective HFSA process is further confirmed by dissipative particle dynamics (DPD) simulations. Moreover, a variety of multi-structural colored CPCs are available by using hydrophobic reagents, combining with the visualized HFSA process. This work presents an easy-to-perform platform for designing orderly structural materials, which will significantly highlight the development of colloidal assembly methodology.
研究领域:
材料科学
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光子学
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胶体化学
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自组装技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Peipei Huang,Qing Chang,Guodong Jiang,Xu Wang,Haipeng Zhu,Qianqian Liu
In this article, a novel metal–organic framework, namely MIL-101(Fe II ), was firstly synthesized via a facile method. In the presence of H 2 O 2 , MIL-101(Fe II ) possesses excellent peroxidase-like activity toward the classical chromogenic substrate, N, N -Diethyl-p-phenylenediamine sulfate salt (DPD). The substitution of Fe 2+ enhances the construction of Fe(II)-oxo nodes and accelerates electrons transfer between DPD and H 2 O 2 , thereby improving the peroxidase-mimicking catalytic activity of MIL-101(Fe II ) nanoenzyme. Additionally, DPD molecules could be adsorbed readily onto the surface of the nanoparticles due to the π–π interaction. The study of Michaelis constant indicates that the MIL-101(Fe II ) exhibits a higher affinity towards DPD (0.16 mM) in contrast to horseradish peroxidase (0.78 mM). In view of the impressive catalytic performance of MIL-101(Fe II ), two reliable monitoring platforms for the rapid detection of H 2 O 2 and glucose were established with extremely low detection limits of 18.04 nM and 0.87 μM in the ranges of 40–5000 nM and 1.2–300 μM, respectively. The study of the catalytic mechanism indicates that DPD oxidation is attributed to the hydroxyl radical (·OH) produced from the decomposition of H 2 O 2 catalyzed by MIL-101(Fe II ). Furthermore, the developed sensor indicates high selectivity and stability and can be effectively appropriate for the detection of H 2 O 2 and glucose in real samples. This work not only provides a novel nanozyme with superior catalytic performance for biological analysis, but also broadens the application field of MIL-101(Fe II ) material.
研究领域:
分析化学
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纳米技术
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生物催化
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生物传感器
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Kang Wang,Zhi Li,Yiming Huang,YaotianTao,Xiao Liang,Xiaoqin Chu,Ning He,Shuangying Gui,Zhenbao Li
In this study, alkanes and sucrose esters are employed to investigate the influence of additives on lyotropic liquid crystal architecture. After molecular dynamic simulations and experiment characterization, we showed how the additives control the structure of LLCs. By controlling the polarity of additives, the phase behavior of LLCs can be engineered to form the required structure. Dissipative particle dynamics (DPD) is introduced for simulating the self-assembly of phytantriol (PT), providing intuitionistic images and structure information, which shows that additives with low-polarity complicate the internal structure of liquid crystal systems. Then the ternary phase diagrams of additives, PT, and water are constructed to systematically study the effects of additives on the phase behavior of LLCs. Consistent with DPD simulation results, there is a certain regularity in the effects of additives on the structure of liquid crystals. The difference in the structure of LLCs is due to the variability in the critical packing parameter (CPP) obtained by changing the polarity of additives. Our findings demonstrate that additives polarity is a key factor in LLCs structure, and may pave a promising avenue for novel LLCs development and translation, determining the self-assembly process and the resulting phase of LLCs.
研究领域:
材料科学
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化学工程
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胶体与表面化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiasheng Lu,Yumeng Sui,Xin Liu,Jiawang Wang,Jiatong Li,Baohua Kong,Qian Chen,Weiwei Yang
The present study aimed to investigate the impact of 4,5-dihydroxy-2,3-pentanedione (DPD; 0, 300, 600, and 900 nmol/L), a crucial precursor in the LuxS/ AI-2 quorum sensing system, on flavour formation by Lactiplantibacillus plantarum HRB1 both in vitro and dry-sausage model. Firstly, L. plantarum HRB1 was selected from 18 strains of lactic acid bacteria due to its superior AI-2 and protease activity. In vitro , DPD significantly increased the activity of the AI-2 and the protease activity of L. plantarum HRB1. In the fermented dry-sausage, free amino acid content was significantly higher in the treatment with 600 nmol/L DPD compared to the other treatments. Notably, the dry sausage treated with 600 nmol/L DPD exhibited the highest concentrations of aldehydes (558.50 μg/kg), ketones (192.41 μg/kg), alcohols (1175.56 μg/kg), acids (202.27 μg/kg), esters (1504.48 μg/kg), and terpenes (1319.82 μg/kg). Sensory analysis revealed that the dry sausage treated with 600 nmol/L DPD had the most pronounced fermented and fatty odour. These results suggest that 600 nmol/L DPD enhances the flavour formation of L. plantarum HRB1. This research provides a theoretical foundation for utilizing the LuxS /AI-2 QS to optimize microbial fermentation processes and improve fermented food quality, especially flavour.
研究领域:
微生物学
·
食品科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Zilong Liu,Ping Liu,Yanxiao Hei,Di Shi,Fengzhi Guo,Xue Li,Wenxiu Leng,Qichao Lv,Weichao Sun
Surfactant emulsions are crucial for stabilizing and enhancing the dispersibility of immiscible substances, enabling efficient mixing and delivery of active ingredients in various industries such as oil recovery, pharmaceuticals, food, and cosmetics. However, a comprehensive understanding of the synergistic mechanism governing surfactant-formed emulsions and their thermal stability remains elusive. Motivated by these knowledge gaps, the self-assembly and interfacial behavior of binary surfactants as well as their influence on the thermal stability of emulsions were systematically investigated. Utilizing a combination of experimental and theoretical approaches, we observed extraordinary synergistic effects in the anionic/zwitterionic and anionic/nonionic surfactant systems, leading to a significant reduction in surface tension and critical micelle concentration, quantitatively assessed by activity parameters and intermolecular interaction parameters. The microscopic mechanism responsible for the observed synergistic effects was investigated by dissipative particle dynamics (DPD) simulations, which could be attributed to steric effects, electrostatic interactions, and hydrophobic interactions. The self-assembly behavior of binary surfactants into micelles was further analyzed to substantiate these findings. Furthermore, the synergistic effects of emulsification performance and thermal stability in binary surfactant systems were quantified by water separation rate and emulsification composite index. Coarse-grained models of oil/water/surfactants were developed to demonstrate the augmented stability of interfacial films to the presence of binary surfactants. The obtained results shed lights on the inherent synergistic mechanism within binary surfactant mixtures, demonstrating their potential in enhancing emulsion thermal stability and paving the way for diverse industrial applications.
研究领域:
化学工程
·
材料科学
·
胶体与表面化学
·
物理化学
·
工业化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）