MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Aiyun Hu,Yijia Zhao,Lun Jiang,Zhiren Gan,Ruoxuan Wang,Cheng Lu,Jian Ding
This study aimed to optimize the extraction process of polysaccharides from seed pumpkins. Following initial single-factor experiments, the extraction process using ultrasonic-assisted enzymatic method was optimized through orthogonal experiments. Further optimization of extraction conditions was conducted using response surface methodology with Box-Behnken design, ultimately identifying the optimal extraction parameters. Additionally, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, monosaccharide component analysis, polysaccharide molecular weight measurement, and NMR analysis were performed. Results indicated that ultrasonic-assisted enzymatic extraction significantly enhanced the yield of polysaccharide, reaching up to 43.65 %. The findings confirm that the combined use of enzymatic hydrolysis and ultrasonication is an effective strategy for polysaccharide extraction.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2014
·
IF 2.468
Ru Song,Rong-bian Wei,Guang-qiang Ruan,Hong-yu Luo
The objective of this study was to identify antioxidative peptides from peptic hydrolysates of half-fin anchovy (HAHp). Furthermore, the possible synergistic effects of identified peptides were evaluated. HAHp were separated using CM FF ion exchange and Sephadex G 25 chromatography. HAHp1-2, one peptide fraction with the highest 1, 1-diphenyl-2-pycrylhydrazyl (DPPH) scavenging activity, was subjected to RP-HPLC coupled with ESI under positive ionization mode. Six peptides, AGDDARPA (771.35 Da), ESAGLHE (741.33 Da), NKVKGELD (901.45 Da), TLWSGAGQTVNT (1233.63 Da), EMSAGLHE (872.37 Da) and WRKKDPLND (1171.60 Da), were identified by ESI-MS/MS analysis. Peptides AGDDARPA (HAHp1-2Ⅰ), ESAGLHE (HAHp1-2Ⅱ), NKVKGELD (HAHp1-2Ⅲ), EMSAGLHE (HAHp1-2Ⅳ) and WRKKDPLND (HAHp1-2Ⅴ) were synthesized to determine in vitro antioxidative activity. Results showed that HAHp1-2Ⅳ and HAHp1-2Ⅴ displayed higher DPPH radical scavenging activities than other three peptides and l -carnosine. Peptide HAHp1-2Ⅲ demonstrated similar reducing power ability as l -carnosine. All synthetic peptides had moderate or negligible metal chelating activities. Additionally, the enhanced DPPH radical scavenging activities or reducing power abilities were observed for the mixture of HAHp1-2Ⅲ, HAHp1-2Ⅳ or HAHp1-2Ⅴ, indicating the possible synergistic effects. These results suggested that the antioxidative peptides derived from HAHp could be used as potential antioxidants in functional foods and nutraceuticals.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
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2014
·Vol. 13
·期 8
·1333-1338
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IF 0.495
C Liu,C Chen,H Ma,E Yuan,Q Li
Purpose: To investigate the physicochemical properties and DPPH radical scavenging activity of gallic acid–lecithin complexMethods: The complex of gallic acid with lecithin was prepared by solvent method. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC)and x-ray diffractometry (XRD). Antioxidant activity was examined b 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay.Results: The UV and IR spectra of the complex showed an additive effect of gallic acid and lecithin, in which the characteristic absorption peaks of gallic acid and lecithin were retained. SEM suggests that gallic acid was dispersed into lecithin while DSC and XRD results for the complex mainly showed the presence of lecithin with the characteristic peaks for gallic acid absent. At all concentrations of the complex (0.2 - 1.0 mg/mL), the DPPH radical scavenging activity of the complex was higher than that of the reference antioxidant, butylated hydroxytoluene (BHT).Conclusion: Gallic acid and lecithin in the complex are combined by a non-covalent bond, and did not form a new compound. The complex is an effective scavenger of DPPH radicals.Keywords: Gallic acid, Lecithin, Complex, Antioxidant
研究领域:
药学
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化学
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食品科学
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DOI
高影响因子
ChemistrySelect
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2022
·Vol. 7
·期 42
·e202203493
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IF 2.307
Xiang Yu,Mengyu Liu,Zaiheng Huang,Yanzhi Tao,Hao Yang,Hongbin Gan,Guangzai Nong
Graphical Quercetin and selenite were combined to form a quercetin selenium complex at 100°C. The mass of the product was 30.3 % of the mass of quercetin and the structure was analyzed in depth. The complex had a DPPH antioxidant activity of 97.91 %, and the prepared skin cream containing 1 % of the complex had an antioxidant activity of 82.4 %. Flavonoid complexes have more biological activities than parent flavonoids, including antioxidant, anticancer, antibacterial, and many others. Selenium is an essential trace element for human body. Therefore, a quercetin-Se complex was synthesized and analyzed in this paper, getting the results as following: (1) A light- yellow product was obtained with the max yield of 30.30 %. (2) The complex molecule contains two quercetin units and a selenoxyl group, called selenious acid acyl diquercetin. (3) It has a strong antioxidant activity with a maximum DPPH scavenging rate of 97.71 %. In addition, we prepared a skin cream containing 1 % quercetin selenium complex, and it has excellent antioxidant activity with a DPPH scavenging rate of 82.4 %. Therefore, the generated selenious acid acyl diquercetin is a potential antioxidant material for skin care products.
研究领域:
化学
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生物学
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药学
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DOI
高影响因子
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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2014
·Vol. 33
·期 1
·159-168
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IF 0.684
Wang Xueqin,Xing Ronge,Liu Song,Yu Huahua,Li Kecheng,Chen Zuoyuan,Li Pengcheng
Mackerel ( Pneumatophorus japonic u s ) proteins were hydrolyzed by five proteases: trypsin, papain, neutrase, acid protease, and flavourzyme. The hydrolysate treated by neutrase exhibited the highest antioxidant activity. Response surface methodology (RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate (MPH) with the highest DPPH radical scavenging activity. The MPH was fractioned using a series of ultrafiltration membranes and five fractions, namely, MPH-I (>10 kDa), MPH-II (10–2.5 kDa), MPH-III (1–2.5 kDa), MPH-IV (0.4–1 kDa), and MPH-V (below 0.4 kDa), were obtained. DPPH radical scavenging activity, reducing power, hydroxyl radical scavenging activity, and the lipid peroxidation inhibition capability of these fractions were evaluated. The fractions in molecular weights <2.5 kDa (MPH-III, MPH-IV, and MPH-V), which occupied 93.4% of the total fractions, showed the strongest antioxidant activity; and the antioxidant activities of the three fractions are similar to each other. Using SP Sephadex C-25 and Sephadex G-25 columns, eight fractions were obtained from the MPH (<2.5 kDa). The isolated peptide I (1 664 kDa) displayed the highest DPPH radical scavenging activity. Therefore, MPH is a potential source of antioxidant peptides.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2021
·Vol. 45
·期 12
·e16074
·
IF 2.19
Xia Li,Guozhu Zhang,Jing Li,Tiemin Jiang,Huiying Chen,Peijun Li,Yuan Guan
In this paper, water extracted polysaccharides from Passiflora edulis peel (WPEP) was degraded by ascorbic acid-hydrogen peroxide (Vc-H 2 O 2 ). The degradation conditions were optimized by response surface methodology (RSM). The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate of degraded WPEP (DWPEP) was 45.38% under optimal conditions (Vc-H 2 O 2 concentration 9.3 mM, temperature 52°C, time 1.4 hr). And the molecular weight (Mw) of DWPEP (38 kDa) was 32.20% (WPEP was 118 kDa). WPEP and DWPEP were mainly composed of galacturonic acid. Their backbone structures, functional groups and β-glycoside linkage patterns are similar, however, still with some differences. Antioxidant tests showed that WPEP and DWPEP had strong DPPH radical scavenging ability (93.03% and 81.70%), moderate hydroxyl radical scavenging ability (53.99% and 26.59%), and low reducing power (0.698 and 0.52). In conclusion, these differences in structures and properties may be an important factor affecting the antioxidant activity of polysaccharides. Novelty impact statement In this paper, the Vc-H 2 O 2 system was used to degrade the polysaccharides extracted from Passiflora edulis peel. The molecular weight, intrinsic viscosity, and rigid conformation changed obviously. The degraded polysaccharides are valuable to expand their application and to improve the practical efficacy.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 7
·6247
·
IF 5.6
Shifan Zhao,Hualei Xue,Yijiong Tao,Kai Chen,Xiao Li,Mi Wang
A novel water-soluble acidic heteropolysaccharide, called PPL-1, was purified fromPueraria lobata. PPL-1 had an average molecular weight of 35 Kad, and it was composed of glucose, arabinose, galactose and galacturonic acid (6.3:0.8:0.8:2.1). In accordance with methylation and nuclear magnetic resonance analyses, PPL-1 primarily consisted of (1→2)-linked α-Araf, (1→4)-linked α-Glcp, (1→)-linked β-Glcp, (1→6)-linked α-Glcp, (1→3,6)-linked α-Galp, (1→)-linked β-GalpA and (1→4)-linked α-GalpA. In terms of bioactivities, PPL-1 exhibited remarkable scavenging ability towards DPPH (1,1-Diphenyl-2-picrylhydrazyl) radicals and moderate activity by enhancing the proliferation rate of RAW 264.7 cells by approximately 30% along with the secretion of NO. This work demonstrates that PPL-1 can be a potential source of immunoenhancers and antioxidants.Keywords:Pueraria lobata;polysaccharide;structure;bioactivity
研究领域:
生物化学
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药理学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APICULTURAL RESEARCH
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2023
·
IF 1.9
Olaoye Solomon Balogun,Anthonia Olufunke Oluduro,Zhiqiang Liu
Volatile oils of propolis obtained from Ondo (Ondo town) and Osun (Ile-Ife) States in Rain forest region of southwest Nigeria were extracted using the hydrodistillation method. The oils were analysed on GC/MS and investigated for DPPH scavenging activities, antibacterial potentials, and inhibitory activities on lipase and alpha glucosidase enzymes. Spectrophotometric methods were used for the DPPH scavenging and enzyme’ inhibitions assays while the agar well diffusion method was employed for the antibacterial test. GC/MS analysis of essential oils obtained from Ondo (BPO) and Ile-Ife (BPI) indicated 80 (97.82%) and 66 (99.31%) compounds respectively. Upon classification, both oils were rich in monoterpenes, sequisterpenes and non-isoprenoid hydrocarbons. Principal chemical components of BPO included trans-α-bergamotene (9.08%), α-copaene (5.83%), cadina-1,4-diene (4.04%), 2-methyl-5-propionylfuran (3.43%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (3.52%), benzaldehyde (2.98%), sulcatone (2.95%), γ-muurolene (2.51%), caryophyllene (2.40%), α-bisabolol (2.09%), α-eudesmol (2.07) and α-curcumene (2.06%) while those of BPI were trans-α-bergamotene (10.14%), citral (5.44%), α-copaene (5.25%), cadina-1,4-diene (5.09%), melonal (3.69%), sulcatone (3.56%), farnesol (2.71%), 3,5,9-trimethyl-deca-2,4,8-trien-1-ol (2.66%), α-curcumene (2.64%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.20%), and α-muurolene (2.20%). Both oils inhibited α-glucosidase and had DPPH scavenging activities better than the standards, however, anti-lipase activity was observed for BPI only. The oils also demonstrated broad spectrum of antibacterial activities.The volatile components of Nigerian bee propolis are being investigated for the first time, and the plethora of biological activities resulting from their diverse chemical composition showed that bee propolis could be a potential source for drug discovery.
研究领域:
药理学
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植物化学
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微生物学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiandong Shen,Bijiang Zhong,Lijun Fu,Bingyan Liu,Wenshui Xia,Qixing Jiang
Aquatic animals are vital source for peptides, while information on antioxidant peptides of Chinese softshell turtle is scarce. The objective of this study was to optimize the production of turtle protein hydrolysate (PTH) by proteinase hydrolysis and evaluate its functional property and antioxidant activity. Results revealed that TPH from turtle protein was hydrolysed using protamex. Under the optimum condition (enzyme-substrate ratio of 3500 U/g, pH 7.0, temperature of 50 °C, and 4 h), TPH had the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, with the degree of hydrolysis (DH) of 21.27 ± 1.32%. Obtained TPH contained a high percentage of essential amino acids and its molecular weight of<3000 Da accounted for 99.61%. The solubility of TPH was more than 80% when pH was below 4.0 or above 5.0, and there was no significant difference in its solubility when temperature was 40–100 °C. Meanwhile, TPH exhibited higher emulsifying activity index and the emulsion stability index at pH 7.0 and 10.0. Antioxidant activity studies showed that DPPH scavenging activity, reducing power and ferrous chelating activity of TPH were attained depending on the concentration of hydrolysate. All results indicated that the functional and antioxidant properties of TPH could be beneficial for its application in functional food.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2015
·Vol. 21
·期 5
·433-439
·
IF 0
Zhu Hongji,Wang Shipeng,Tian Li,Zhang Hua
Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes(Papain, Pepsin, Trypsin and Neutrase, respectively)were investigated. The Trypsin hydrolysate obtained by hydrolysis exhibited the highest 1,1-dipheny-l-2-picrylhydrazyl(DPPH)radical scavenging activity(DRSA)compared with other hydrolysates. Response surface method ology(RSM), based on Box-Behnken design, was used to study the influence of hydrolysis conditions on the DRSA. The optimal hydrolysis conditions were as follows: pH 7.38, temperature 48.2°C and enzyme/substrate(E/S)ratio 2 840 U/g. Under these conditions, the maximum DRSA was(22.85 ± 0.57)%,. The experimental values agreed with the value (23.09%,)predicted by the model within a 95%, confidence interval. By using gel filtration chromatography and reversed-phase high performance liquid chromatography(RP-HPLC), antioxidant peptide(D2-P)was isolated from flatfish skin protein hydrolysates(FSPH)and could exhibit a(54.28 ± 1.37)%, scavenging activity on DPPH radical at the concentration of 5 mg/mL. This is the first report of a scientific basis for the preparation of antioxidant peptides from flatfish skin. The results suggested that the antioxidant peptides can be exploited into functional foods or used as a novel source of nutraceuticals.
研究领域:
食品科学
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生物化学
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产品关联
DOI
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xingzhu Tang,Lingling Wang,Yajie Zhang,Chaofan Sun
Antioxidant activity, as a topic of current interest, is discussed together with the excited state intramolecular proton transfer (ESIPT) process for three flavonoid derivatives, based on density functional theory (DFT)and time-dependent DFT (TD-DFT) methods, as well as DPPH free radical scavenging assay. The potential energy curves and transition states demonstrate that the three molecules can undergo only single proton transfer in the excited state, and all of them are ultrafast ESIPT processes. The absorption spectra of all the molecules show effective protection against UV radiation with low fluorescence intensity, especially Baicalein (Bai), which demonstrates their great potential for sunscreen applications. The density of states, HOMO energy values, global and local indices reveal that the antioxidant activity of the molecules after ESIPT process is enhanced, with Bai having the highest antioxidant activity, which is significantly attributed to the number and position of phenolic hydroxyl groups. Moreover, by comparing the DPPH free radical scavenging activity under the dark and UV radiation conditions, the radical scavenging activity (RSA) value in the UV radiation is remarkably higher than that in the dark condition, in which Bai achieves RSA value of 93.4%. Overall, the antioxidant activity of all three ESIPT-based flavonoid derivatives, especially Bai, is significantly elevated in the keto* form, which reinforces the significant relationship between antioxidant activity and ESIPT process, and provides new application prospects for molecules with ESIPT properties.
研究领域:
化学
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生物学
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产品关联
DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
·
2014
·
IF 0
Pei-Pei WANG,Guo-Wei ZHAO,Wen XIA,En-Ji HAN,Lan XIANG
This study was conducted to investigate the chemical constituents of Piper wallichii (Miq.) Hand.-Mazz. and evaluate their biological activity. Compounds were isolated by various column chromatographic methods, and their structures were elucidated on the basis of physical characteristics and spectral data. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging activity and acetylcholinesterase (AChE)-inhibitory activity of the compounds were evaluated. Five compounds were obtained and identified as 8- C -β-D-glucopyranosylkaempferol-3- O -β-D-glucopyranoside ( 1 ), 1, 2-dihydro-6,8-dimethoxy-7-hydroxy-1-(3, 5-dimethoxy-4-hydroxyphenyl)- N 1 , N 2 -bis-[2-(4-hydroxyphenyl)ethyl]-2, 3-naphthalene dicarboxamide ( 2 ), goniothalactam ( 3 ), aristololactam A IIIa ( 4 ) and piperlonguminine ( 5 ). Compound 1 was a new flavonol C -glycoside, 2 was a rare lignanamide, which was isolated from the family Piperaceae for the first time, and compound 3 was isolated from this plant for the first time. Among them, 2 showed potent DPPH-scavenging activity, with IC 50 of 31.38 ± 0.97 μmol·L −1 ; Compounds 2 , 3 , and 4 showed AChE inhibitory activity at 100 μmol·L −1 , with inhibition rates of 28.57% ± 1.47%, 18.48% ± 2.41% and 17.4% ± 3.03%, respectively.
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
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