TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
·
2014
·Vol. 13
·期 8
·1333-1338
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IF 0.495
C Liu,C Chen,H Ma,E Yuan,Q Li
Purpose: To investigate the physicochemical properties and DPPH radical scavenging activity of gallic acid–lecithin complexMethods: The complex of gallic acid with lecithin was prepared by solvent method. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC)and x-ray diffractometry (XRD). Antioxidant activity was examined b 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay.Results: The UV and IR spectra of the complex showed an additive effect of gallic acid and lecithin, in which the characteristic absorption peaks of gallic acid and lecithin were retained. SEM suggests that gallic acid was dispersed into lecithin while DSC and XRD results for the complex mainly showed the presence of lecithin with the characteristic peaks for gallic acid absent. At all concentrations of the complex (0.2 - 1.0 mg/mL), the DPPH radical scavenging activity of the complex was higher than that of the reference antioxidant, butylated hydroxytoluene (BHT).Conclusion: Gallic acid and lecithin in the complex are combined by a non-covalent bond, and did not form a new compound. The complex is an effective scavenger of DPPH radicals.Keywords: Gallic acid, Lecithin, Complex, Antioxidant
研究领域:
药学
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化学
·
食品科学
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DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 11
·3170-3178
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IF 2.245
Wang Liao,Chen Yisheng,Ye Zhiyang,Hellmann Bernhard,Xu Xueming,Jin Zhengyu,Ma Qinqin,Yang Na,Wu Fengfeng,Jin Yamei
The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert -butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 ( v / v / v ) to 70 mm solvent front, the plate was subjected to a standardized 1,1-diphenyl-2-picrylhydrazyl assay. In addition to synoptical eye inspection, accurate quantification was realized by modified densitometric measurements: fluorescence mode, excitation wavelength 530 nm (D 2 and W lamp) without optical filter, which offered satisfactory sensitivity (8.5–17.5 mg/kg) and acceptable linearity ( R 2 > 0.999 within 50–200 ng/zone). Moreover, the established method was validated with edible oil samples, against EU Directive 2006/52/EC. Apart from that, the unambiguous confirmation of positive results was conveniently achieved by TLC-MS interface-mediated mass spectrometry. Featuring the merits of screening conception, the proposed method not only reached the goal of accurate quantification and conclusive identification of multi-phenolic antioxidants, but also excellently balanced the simplicity, detectability, and throughput of the screening workup. Therefore, it might be an attractive alternative to conventional methods.
研究领域:
食品安全
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分析化学
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营养学
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DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2021
·
IF 2.162
Guilong Yan,Yuzhen Zhou,Yonghong Hu,Liqin Zhao,Wei Wang
Counter-current chromatography (CCC) target-guided by on-line HPLC with post-column DPPH assay was established for efficient screening and isolation of large amount of antioxidants from Eupatorium lindleyanum DC. On-line HPLC with post-column DPPH reaction was used to screen the antioxidants and optimize the biphasic solvent system of CCC, then the targeted peaks were purified using CCC. In the present study, three compounds, nepetin, cirsiliol and jaceosidin, were targeted and successively separated from n-butanol fraction of E. lindleyanum DC. by this strategy. All three compounds showed strong DPPH radical scavenging activity. These results confirmed that the strategy would be an efficient and effective method to isolate antioxidants from complex mixtures.
研究领域:
药用植物化学
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分离分析技术
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PubMed
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2019
·Vol. 56
·期 9
·4009-4015
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IF 1.85
Qin Hao,Li Bao-cai,Dai Wei-feng,Xiang Cheng,Qin Yi,Jiao Shi-yun,Zhang Mi
Volatiles have been regarded as active substances in many foods, whose chemicals can be analyzed by GC–MS qualitatively and quantitatively. However, the activities of volatiles are often studied as a whole, and it has no an effective method to determine that which molecule is active in volatiles by far. In order to identify the antioxidant molecules in volatiles, a rapid determination method was developed by GC–FID/MS combined with DPPH radical reaction in this study. Three antioxidant molecules were identified and validated among 20 components in rose tea infusion. Their activity validation and the methodological evaluation indicated this method could be used for distinguishing antioxidant molecules in volatiles rapidly and effectively.
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
Catalysts
·
2019
·Vol. 9
·期 10
·813
·
IF 3.444
Tao Wen,Doudou Yan,Jie Meng,Jian Liu,Haiyan Xu
With co-reduction method, a new nanocomposite consisting of Cu and Pt (CuPt) was prepared in household. The morphology of CuPt alloy was characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The diameter of CuPt was ca. 125 nm measured by dynamic light scattering (DLS). The atom ratio of Cu to Pt was determined to be about 1.2 by energy-dispersive X-ray (EDX). Enzyme-like activities of CuPt, including peroxidase (POD)-like and ascorbic acid oxidase (AAO)-like activities were examined with UV-Vis-NIR spectrometer. The CuPt was found to interact with α, α-diphenyl-β-picrylhydrazyl (DPPH) in the presence or absence of AA. With irradiation by light emitting diode (LED) light, the photocatalysis effect of CuPt on DPPH was investigated. With the addition of histidine, it was proved that singlet oxygen had an important role in the interaction involving CuPt. The new nanocomposite and the properties suggest various potentials of application.Keywords:CuPt;peroxidase (POD)-like;ascorbic acid oxidase (AAO)-like;DPPH
研究领域:
纳米生物学
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催化化学
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自由基生物学
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DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liqi Ai,Lei Liu,Lin Zheng,Yang Liu,Baoguo Sun,Guowan Su,Jucai Xu,Yajun Chen,Mouming Zhao
Food-derived antioxidant peptides have become the focus of research due to their high safety and low cost. However, the discovery is suffering from a low efficient and empirical approach, involving multi-step off-line separation and identification. In this work, an on-line stop-flow RPLC × SEC -MS/DPPH radical scavenging activity analysis system was developed. For optimization, the conditions: 10 m reaction loop, 200 μM DPPH radical concentration, 40℃ temperature and 0.06 % formic acid were recommended. The system was fully validated by its application in glutathione analysis. The system was further applied in analysis of complex mixed standards, and the dipeptides GC (Gly-Cys) and CW (Cys-Trp) with relatively strong DPPH radical scavenging activity were validated. Maize protein hydrolysates were used for tests and the peptide AC (Ala-Cys) of high probability with strong DPPH radical scavenging activity was identified, demonstrating a high potential of the system. This would help to facilitate the discovery of antioxidative peptides in the future.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Yingjie Bao,Xiaopu Ren,Yuxia Zhu,Yawei Zhang,Zengqi Peng,Guanghong Zhou
The effects of 7 spice extracts on lipid radical formation were first collectively studied in situ in roasted beef by electron spin resonance (ESR). And the correlations among the ESR and conventional indexes including 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity, reducing power, oxygen radical absorption capacity (ORAC), conjugated diene value (CDV) and thiobarbituric acid-reactive substances (TBARS) were also analysed. Clove was observed the highest scavenging lipid radical and DPPH radical capacity, and rosemary showed the highest peroxyl radical scavenging capacity, while tert-butylhydroquinone (TBHQ) significantly increased lipid radicals in roasted beef ( p < 0.05). ESR showed significant correlations with the DPPH and CDV assays ( p < 0.05) but no correlation with the ORAC and TBARS assays ( p > 0.05), indicating that with the addition of spice extracts, the lipid radical formation in roasted beef could be predicted by the DPPH assay but not be appropriate to evaluate the oxidation stability in roasted beef.
研究领域:
食品科学
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化学
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营养学
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DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 12
·4760
·
IF 4.6
Feifei Wang,Jie Tan,Ruixiang Jiang,Feifei Li,Renqing Zheng,Linjun Yu,Lianzhong Luo,Yongbiao Zheng
In recent years, wild morel mushroom species have begun to be widely cultivated in China due to their high edible and medicinal values. To parse the medicinal ingredients, we employed the technique of liquid-submerged fermentation to investigate the secondary metabolites ofMorehella importuna. Two new natural isobenzofuranone derivatives (1–2) and one new orsellinaldehyde derivative (3), together with seven known compounds, including oneo-orsellinaldehyde (4), phenylacetic acid (5), benzoic acid (6), 4-hydroxy-phenylacetic acid (7), 3,5-dihydroxybenzoic acid (8),N,N′-pentane-1,5-diyldiacetamide (9), and 1H-pyrrole-2-carboxylic acid (10), were obtained from the fermented broth ofM. importuna. Their structures were determined according to the data of NMR, HR Q-TOF MS, IR, UV, optical activity, and single-crystal X-ray crystallography. TLC-bioautography displayed that these compounds possess significant antioxidant activity with the half DPPH free radical scavenging concentration of 1.79 (1), 4.10 (2), 4.28 (4), 2.45 (5), 4.40 (7), 1.73 (8), and 6.00 (10) mM. The experimental results would shed light on the medicinal value ofM. importunafor its abundant antioxidants.Keywords:Morehella importuna;isobenzofuranone;orsellinaldehyde;antioxidant;microbial fermentation
研究领域:
真菌学
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药理学
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生物化学
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DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
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2020
·
IF 6.513
Subramanian Siva,Changzhu Li,Haiying Cui,Venkatasamy Meenatchi,Lin Lin
Essential oils derived from medicinal plants are prosperous sources of active components having high biological potential. Cuminaldehye and isoeugenol, are hydrophobic essential oil components (EOC), are showing drastic limitations in their applications by low water solubility and the respective volatility. Methyl- β -cyclodextrin inclusion complexes (M β CD-ICs) were prepared in aqueous solution and in solid state with the EOC via the ultrasonication method, an energy saving, high efficiency and eco-friend technique, aim to extend their aqueous solubility and biological properties. UV–Vis absorption, fluorescence, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), proton nuclear magnetic resonance ( 1 H NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, powder X-ray diffraction (PXRD) and computational simulations confirmed the formation of EOC/M β CD-ICs. Result of solubility studies proved the enhanced solubilization of EOC in the presence of M β CD in aqueous and double reciprocal profiles substantiated the guest/host stoichiometry of 1:1. TGA and DSC studies indicated the improved stability of EOC in M β CD-ICs. The efficiency of ICs in terms of the antioxidant activity was verified and the IC displayed higher antioxidant activity compared to that of free EOC, as determined by free radical scavenging assay. Finally, the antibacterial effect of EOC/M β CD-ICs against gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacteria was demonstrated. Overall results not only revealed the potential of M β CD on the bioavailability, solubility and stability, but also that the intensification caused by the IC may be greater that the antioxidant and antibacterial effects of the selected EOC for this study.
研究领域:
药物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2013
·
IF 2.906
Jia Sun,Jin Yu,Pei-Cheng Zhang,Feng Tang,Yong-De Yue,Ya-Nan Yang,Zi-Ming Feng,Xue-Feng Guo
Caulis Bambusae in Taenia is a medicinal preparation from Bambusa tuldoides Munro consisting of skinless slices of the stem (bamboo shavings) and used as a traditional health food in tea, wine, and soup in Asia. Three novel lignans, (−)-7′-epi-lyoniresinol 4,9′-di-O-β-d-glucopyranoside (7), (−)-lyoniresinol 4,9′-di-O-β-d-glucopyranoside (8), bambulignan A (10), and seven known lignan compounds (1–6 and 9) were isolated from Caulis Bambusae in Taenia. The structures of the lignans were determined by detailed spectroscopic analysis (HRESIMS, HSQC, HMBC, NOE). All the isolated lignans were tested for antioxidant activities by DPPH and FARP assays. The results showed that the compounds (+)-lyoniresinol 9′-O-β-d-glucopyranoside (1) and (−)-7′-epi-lyoniresinol 9′-O-β-d- glucopyranoside (9) have strong free radical scavenging activity and reducing power.
研究领域:
药用植物学
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天然产物化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2026
·
IF 2.5
Qiwen Shi,Haiping Xiang,Ying Fan,Chunyan Wu,Shengqiang Tong
Dual high-resolution profiling integrated α-glucosidase and DPPH assays. • Twelve bioactive constituents were identified from Lygodium japonicum roots. • Two new compounds were isolated and structurally characterized. • Multi-technique studies revealed their interactions with α-glucosidase. This study aimed to isolate and characterize α-glucosidase inhibitory and antioxidant compounds from the roots of Lygodium japonicum and to investigate their interactions with α-glucosidase. Dual high-resolution α-glucosidase inhibition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging profiling was employed, which led to the identification of 12 constituents associated with α-glucosidase inhibitory and/or antioxidant activities from L. japonicum roots, including two new compounds, 6-hydroxy-7-(hydroxymethyl)-2-isopropyl-1,4-naphthoquinone ( 7 ) and 1,1′-dimer of 2,3-dihydroxy-4-methoxydibenzofuran ( 10 ). Several isolated compounds showed notable bioactivities, with compounds 7 , 8 , and 10 exhibiting measurable α-glucosidase inhibitory activity (IC 50 < 11 μM) and compounds 1 , 2 , 4 , 5/6 , and 10 displaying robust DPPH radical scavenging activity (IC 50 < 20 μM). Enzyme inhibition kinetics, fluorescence spectroscopy, circular dichroism spectroscopy, and molecular docking were employed to characterize three of the most potent α-glucosidase inhibitors and to reveal their potential binding interactions with the enzyme. Overall, this study highlights the utility of dual high-resolution bioactivity profiling for identifying chemically defined constituents that contribute to the in vitro α-glucosidase inhibitory and antioxidant activities of L. japonicum roots. Download: Download high-res image (160KB) Download: Download full-size image
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PubMed
高影响因子
Forests
·
2024
·Vol. 15
·期 2
·236
·
IF 2.4
Liping Yu,Wang Cheng,Meifen Tian,Zhigang Wu,Xiaoli Wei,Xing Cheng,Mingwei Yang,Xuan Ma
The medicinal value ofP. zhennanhas been documented in traditional Chinese medicine books. The aim of this paper was to study the antioxidant activity of alcoholic extracts ofP. zhennanleavesusing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-phenyl-4,4,5,5-tetramethylimidazolineoxyl-1-oxyl-3-oxide (PTIO) radical scavenging and ferric ion reducing antioxidant power (FRAP) assays. The active components of the leaves were identified via headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC-MS). The results showed that the scavenging rate of DPPH was 94.67%with an EC50 value of 0.674 mg/mL at a concentration of 2 mg/mL. The maximum scavenging rate was 47.40% at a Trolox equivalent of 0.33 mg TE/mL for PTIO radicals. The FRAP reached 84.80% at 0.20 mg/mL concentration. The results confirmed the strong antioxidant activity of the extracts. Furthermore, 44 compounds, mostly terpenoids, obtained from the alcoholic extracts ofP. zhennanleaves were analyzed using HS-SPME-GC-MS and 15 of these compounds had a relative content exceeding 1%. The strong antioxidant activity of the alcoholic extracts ofP. zhennanleaves could be attributed to the presence of copaene (33.97%), β-caryophyllene (4.42%), δ-cadinene (11.04%), γ-muurolene (4.78%), cis-calamenene (2.02%), linalool (1.04%), α-pinene (1.46%), borneol acetate (1.5%), and γ-terpinene (0.66%). This study demonstrates the potential medicinal value of alcoholic extracts ofP. zhennanleaves.
研究领域:
植物学
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药理学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）