ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Jing Zhu,Liutao Chen
Fluorescent dye DITO-1 has almost no fluorescence in the absence of nucleic acid . G bases in single strand DNA can induce maximum fluorescent enhancement followed by the A bases when it binds the DITO-1. However, the incorporation efficiency of the dATP was higher than dGTP in terminal transferase (TdT) polymerization. As a consequence, ploy (A) n , rather than ploy (G) n via TdT polymerization had the superior photoluminance when it binded DITO-1 fluorescent dye. Here, we developed a high selective and sensitive sensing strategy for assaying TdT and T4 polynucleotide kinase activity (T4 PNK) based on the ploy (A) n -DITO-1 fluorescent probe. An increasing amounts of TdT enzyme could promote the distinct incorporation of dATP on the DNA primer and form poly (A) n ssDNA with a difference in length. A good linear relationship between the ΔF and the concentrations of TdT in a range of 0.2–50 U/mL was obtained and the detection limit was 0.05 U/mL. Based on the experimental results for TdT, we further expanded the application of this method for detection of a series of concentrations of T4 PNK. The ΔF and the logarithm concentrations of T4 PNK in the range of 0.1–10 U/mL showed a good linear response and the detection limit of 0.02 U/mL was obtained. In addition, the detection of T4 PNK in Hela cell lysate was achieved, demonstrating that the proposed method had the potential application in complex system. The ploy (A) n -DITO-1 fluorescent probe had the excellent properties of one-step readout, robustness for target detection in complex system, and easiness operation, and showed the great potential in clinical diagnostics, inhibitor screening, and drug discovery.
研究领域:
分子生物学
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生物化学
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分析化学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2025
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IF 2.6
Han Qing,Li Zhibo,He Mengyuan,Wei Zhengwen,Song Shuhao,Wang Jiacheng,Wang Wei
This study proposes a simple method for preparation Schiff base polymers (DATP) and its metal complexes (M-DATPs, M = Cu 2+ , Fe 2+ ) as electrode materials for supercapacitors. The condensation reaction involves mixing 1,4-diaminoanthraquinone, terephthalaldehyde, and metal chlorides in trifluoroacetic acid at room temperature. M-DATPs possess larger surface area, unique morphology, and suitable conductivity in comparison with DATP. The electrochemical performance of the materials was evaluated in a 6 mol L −1 KOH electrolyte using cyclic voltammetry (CV), galvanostatic charge–discharge (GCD), and electrochemical impedance spectroscopy (EIS). And Cu-DATPs were used as the positive electrode and activated carbon (AC) as the negative electrode to assemble the supercapacitor Cu-DATPs//AC, which was investigated through the dual-electrode testing. The results indicate that M-DATPs possess excellence electrochemical performance, the specific capacitance of sample Cu-DATPs get 853.87 F g −1 at a current density of 0.5 A g −1 . And the galvanostatic charge–discharge (GCD) research illustrate that sample Cu-DATPs have good cycling stability. In addition, the energy density of Cu-DATPs//AC is 64.055 Wh kg −1 , and the power density is 288.46 W kg −1 . This study provides a novel strategy for the synthesis of conjugated Schiff base polymers and metal ion coordination to prepare electrode materials with excellent electrochemical performance.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yalan Hu,Yueru Shi,Yuefan Liu,Yixin Kuang,Suxin Zhou,Luyi Chen,Sai Ouyang,Ningbo Zhou,Juan Zheng,Gangfeng Ouyang
Aromatic esters have been widely used in daily life with non-ignorable dangers, such as plasticizer , flavor , and preservative. Their wide applications and corresponding hazards caused by overuse have promoted the rapid development of sensitively analytical method for effective regulation. However, the variety makes it challenging for broad-spectrum and simultaneous extraction of diverse aromatic esters from the highly complex cosmetic samples. To our delight, a covalent organic framework, named DaTp (1, 3, 5-triformylphloroglucinol-2, 6-diaminoanthracene), possessing high specific surface, excellently thermochemical stability, and abundant electron-rich heteroatoms , has been synthesized and fabricated as a competitive solid phase microextraction coating for extracting the trace analytes with diverse polarity, through the hydrophobic interaction, π-π conjugation and hydrogen bond . Herein, this self-made SPME fiber has been further coupled with gas chromatography-tandem mass spectrometry (GC-MS) to determine the multi-polar aromatic esters in cosmetics packaged with plastic. This developed analytical method showed wide liner ranges, low limits of detection, good repeatability and reproducibility. Finally, the aromatic esters in four cosmetic samples were quantified precisely with satisfactory recoveries (80.7%–118%).
研究领域:
分析化学
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材料科学
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环境科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Chenyang Mei,Dajie Lin,Chengchao Fan,Aili Liu,Shun Wang,Jichang Wang
A Hg 2+ electrochemical biosensor is developed by integrating thymine–Hg 2+ –thymine (T–Hg 2+ –T) base pairs for the high selectivity with surface-initiated enzymatic polymerization (SIEP) for signal amplification . The fabrication begins with the covalent conjugation of capture DNA probe labeled with thiol at its 3′terminal onto the gold electrode. The presence of Hg 2+ leads to DNA hybridization, in which complementary DNA was captured onto the biosensor surface, which subsequently catalyzed the addition of deoxynucleotides (dNTP) containing biotinlated 2′-deoxyadenosine 5′-triphosphate (biotin-dATP) by terminal deoxynucleotidyl transferase (TdT). The binding between biotin and strepavidin leads to the attachment of a large number of strepavidin functionalized silver nanoparticles (strepavidin-AgNPs), which could generate electrochemical stripping signal of silver to monitor the concentration of Hg 2+ in KCl solution. Through utilizing the T–Hg 2+ –T selectivity and SIEP amplification, this assay method can detect aqueous Hg 2+ with a wide linear range from 0.05 nM to 100 nM and a detection limit of 0.024 nM. The application of this sensor in the analysis of drinking water demonstrates that the proposed method works well for real samples.
研究领域:
分析化学
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环境科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
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IF 5.76
Xinxin Ren,Qingqing Zhang,Lingxia Qin,Kaiyue Hu,Zhiyong Guo,Sui Wang,Yufang Hu
In this study, a checkerboard arranged G4 nanostructure-supported electrochemical platform is developed well for the application to unique bio-enzymes examination. Herein, we focus on the two bio-enzymes involving histone acetyltransferase (HAT) and terminal deoxynucleotidyl transferase (TdT); the former leads to the acetyl transfer of acetyl coenzyme A to the lysine residue of the substrate peptide and the latter achieves the polymeric extension of DNA without template under a unique pool of dATP and dGTP (4: 6). A complex of antibody and short DNA is introduced onto the electrode surface based on the affinity interaction between acetyl in acetylated peptide and its antibody. and used for initiating reaction. Then, TdT-yielded rich-G sequence is formed to act as the backbone of checkerboard, and subsequently free G-DNAs can be stacked continually on the backbone under Mg 2+ . An excellent electrocatalytic signal to H 2 O 2 emerges noticeably, which is related with the activity of HAT p300 and TdT with a low detection limit of 2.3 pM and 0.38 mU/mL, respectively. Finally, this strategy is also used successfully for the inhibitor screening and complex sample analysis, which has important implications for the advancement of HAT- and TdT-related electrochemical bioassay and drug discovery.
研究领域:
生物化学
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电化学
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分子生物学
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分析化学
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药物发现
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）