Nuclear Engineering and Technology
·
2023
·
IF 2.7
Guanghui Wang,Yaorui Li,Mingjian He,Meng Zhang,Yang Gao,Hui He,Caishan Jiao
In spent fuel reprocessing, UO 2 (DBP) 2 (U-DBP) can be deposited in stainless steel equipment. U-DBP must be removed by dissolution and the process must not cause corrosion to stainless steel. This study was conducted to find the best scheme for dissolution. U-DBP was manufactured by the titrimetric sedimentation method. The effects of different factors on the dissolution of U-DBP were investigated. For example, solid-liquid ratio, hydrazine carbonate solutions with different mass components, mixed solutions containing different concentrations of H 2 O 2 , and different carbonates. The results indicated that U-DBP does not have a regular crystal morphology. With the increase of the solid-liquid ratio and the mass fraction of hydrazine carbonate, the concentration of U(VI) at the dissolution equilibrium increases gradually. The addition of H 2 O 2 has a great promotion effect on the dissolution. However, when the concentration of H 2 O 2 is greater than 0.5 M, the dissolution solution may have an erosive effect on the stainless steel. (NH 4 ) 2 CO 3 can increase the dissolution capacity of dissolved U-DBP, but it may also accelerate the corrosion of stainless steel.
研究领域:
化学工程
·
材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2015
·
IF 4.143
Hai-Ming Zhao,Huan Du,Lei Xiang,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Gang Cao,Ming-Hung Wong
To increase understanding on the mechanisms of cultivar difference in contaminant accumulation in crops, this study was designed to compare the physiological responses to di- n -butyl phthalate (DBP) exposure between low ( Lvbao70 ) and high ( Huaguan ) DBP cultivars of Chinese flowering cabbage ( Brassica parachinensis L.). Under high DBP exposure, significant differences in various physiological responses were observed between the two cultivars, which might account for the variation in DBP accumulation. Ultrastructure observation also showed different alterations or damages in the mesophyll cell structures between both cultivars, especially for the chloroplast disintegration, starch grain quantity, and plastoglobuli accumulation. Compared with Huaguan , Lvbao70 suffered greater decreases in biomass, chlorophyll content, carbon assimilation, gas exchange parameters, photosynthetic electron transport capacity, and antioxidase activities, which would have resulted in a great reduction of photosynthetic capacity. Although Lvbao70 enhanced energy dissipation and activities of some antioxidant enzymes, they did not provide sufficient protection against oxidative damage caused by DBP. The result suggested that the lower DBP tolerance of Lvbao70 might be associated with its poor physiological performances, which was responsible for its lower DBP accumulation to protect itself from toxicity. Additionally, Lvbao70 had a significantly lower transpiration rate and stomatal conductance than Huaguan , which might be the factors regulating DBP-accumulation variation.
研究领域:
环境科学
·
植物生理学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2017
·
IF 4.9
Nai-Xian Feng,Jiao Yu,Ce-Hui Mo,Hai-Ming Zhao,Yan-Wen Li,Bing-Xiao Wu,Quan-Ying Cai,Hui Li,Dong-Mei Zhou,Ming-Hung Wong
Phthalic acid esters (PAEs) are a group of recalcitrant and hazardous organic compounds that pose a great threat to both ecosystem and human beings. A novel endophytic strain YJB3 that could utilize a wide range of PAEs as the sole carbon and energy sources for cell growth was isolated from Canna indica root tissue. It was identified as Bacillus megaterium based on morphological characteristics and 16S rDNA sequence homology analysis. The degradation capability of the strain YJB3 was investigated by incubation in mineral salt medium containing di-n-butyl-phthalate (DBP), one of important PAEs under different environmental conditions, showing 82.5% of the DBP removal in 5 days of incubation under the optimum conditions (acetate 1.2 g·L − 1 , inocula 1.8%, and temperature 34.2 °C) achieved by two-step sequential optimization technologies. The DBP metabolites including mono-butyl phthalate (MBP), phthalic acid (PA), protocatechuic acid (PCA), etc. were determined by GC–MS. The PCA catabolic genes responsible for the aromatic ring cleavage of PCA in the strain YJB3 were excavated by whole-genome sequencing. Thus, a degradation pathway of DBP by the strain YJB3 was proposed that MBP was formed, followed by PA, and then the intermediates were further utilized till complete degradation. To our knowledge, this is the first study to show the biodegradation of PAEs using endophyte. The results in the present study suggest that the strain YJB3 is greatly promising to act as a competent inoculum in removal of PAEs in both soils and crops.
研究领域:
环境微生物学
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生物修复
·
基因组学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Nai-Xian Feng,Yu-Xi Feng,Qi-Feng Liang,Xin Chen,Lei Xiang,Hai-Ming Zhao,Bai-Lin Liu,Gang Cao,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming Hung Wong
Phthalate acid esters (PAEs) are environmentally ubiquitous and have aroused a worldwide concern due to their threats to environment and human health. Di-n-butyl phthalate (DBP) is one of the most frequently observed PAEs in the environment. In this study, a novel bacterium identified as Pseudomonas sp. YJB6 that isolated from PAEs-contaminated soil was determined to have strong DBP-degrading activity. A complete degradation of DBP in 200 mg/L was achieved within 3 days when YJB6 was cultivated at 31.4 °C with an initial inoculation size of 0.6 (OD600) in basic mineral salts liquid medium (MSM), pH 7.6. The degradation curves of DBP (50–2000 mg/L) fitted well the first-order kinetics model, with a half-life ( t 1/2 ) ranging from 0.86 to 1.88 d. The main degradation intermediates were identified as butyl-ethyl phthalate (BEP), mono-butyl phthalate (MBP), phthalic acid (PA) and benzoic acid (BA), indicating a new complex and complete biodegradation pathway presented by YJB6. DBP might be metabolized through de-esterification, β-oxidation, and hydrolysis, followed by entering the Krebs cycle of YJB6 as a final step. Strain YJB6 was successfully immobilized with sodium alginate (SA), polyvinyl alcohol (PVA), and SA-PVA. The immobilization significantly improved the stability and adaptability of the cells thus resulting in high volumetric DBP-degrading rates compared to that of the freely suspended cells. In addition, these immobilized cells can be reused for many cycles with well conserved in DBP-degrading activity. The ideal DBP degrading ability of the free and immobilized YJB6 cells suggests that strain YJB6, especially the SA-PVA+ YJB6 promises great potential to remove hazardous PAEs.
研究领域:
环境微生物学
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生物修复
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化学工程
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Jing Feng,Chengliang Zhou,Qian Yang,Zhi Dang,Lijuan Zhang
Inhibition of tailings oxidation could availably control the generation of acid mine wastewater from its source. Organosilanes serving as a high-efficiency inhibitor of the oxidation of pyrite, bring some problems including safety hazards caused by large amounts of organic solvents, difficult high-temperature curing, poor long-term properties, and so on. In our work, the PropS-SH/Ce (dbp) 3 (PS/Ce (dbp) 3 ) passivator with excellent passivation performance and self-healing properties was prepared by choosing 3-mercaptopropyltrimethoxysilane (PropS-SH) and dibutyl phosphate (Ce (dbp) 3 ) as the main passivating agent and the repair agent, respectively. We reduced the ratio of ethanol to water by adjusting the pH of the organosilane condensation and also achieved room-temperature curing by extending the curing time. Electrochemical and chemical leaching experiments results showed that the most appropriate addition of Ce (dbp) 3 was 0.2 wt% for enhancing the passivation performance of the passivated coating. In a 6-month chemical leaching experiment, the PS/Ce (dbp) 3 -0.2 passivation coating cured at room temperature showed a better passivation effect and maintained 90.55% and 78.54% of total Fe and SO 4 2− passivation efficiencies. The passivation and self-healing mechanisms were investigated by FT-IR, XPS , 29 Si NMR, and other characterization methods, which were as follows: silane formed a cross-linked mesh structure by Si–O–Si bonding, in which Ce (dbp) 3 was physically filled. And the Si–OH on the surface of the passivation film formed Fe–O–Si bonds with the hydroxyl groups on the surface of the pyrite, thus attaching to the surface of the pyrite and isolating the oxidation medium. When the passivation coating was locally damaged, the oxidation reaction caused a change in pH, which accelerated the dissolution of Ce (dbp) 3 in the passivation layer. Ce 3+ underwent a valence change and formed a CeO 2 precipitate, while dbp − could form a complex with Fe 2+ on the pyrite surface, both of which worked together to repair the broken passivation coating and prevent the oxidation reaction.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY
·
2021
·
IF 4.221
Tingting Zhou,Yueyan He,Yujie Qin,Bing Wang,Hongmao Zhang,Shumao Ding
The large conjugated π bond in the molecular structure of carbon nanotubes (CNTs) interacts with the benzene ring structure in di (n-butyl) phthalates (DBP) through a π - π bond . Compounds of CNTs and DBP form easily, becoming another environmental pollutant of concern. We explore whether CNTs entering animals slow down the degradation of the DBP adsorbed in the CNT cavity, thereby prolonging the "hormonal activity" of DBP. In our study, male BALb/c mice were used as experimental subjects divided into four groups: the control group; the multi-walled carbon nanotubes (MWCNTs) exposure group (10mg/kg/d); the DBP exposure group (2.15 mg/kg/d); and the compound exposure group (MWCNTs + DBP). After 30 days of exposure, the mice were sacrificed and their spleens used for immunotoxicology study. The results showed that the exposure groups exhibited splenomegaly and suffered severe oxidative damage to the spleen. In the compound exposure group: levels of IgA and IgG in the serum of the mice changed, and were significantly different from levels in both the MWCNTs and DBP exposure groups (p <0.05); the pathological sections of the spleen showed that the boundary between the white pulp area (WP) and the red pulp area (RP) was blurred, that the cell arrangement was loose, and that more red blood cells were retained in the spleen. Proteomics mass spectrometry analysis showed that compared with the control group, 70 proteins were up-regulated and 27 proteins were down-regulated in the MWCNTs group, 36 proteins were up-regulated and 23 proteins were down-regulated in the DBP group, 87 proteins were up-regulated and 21 proteins were down-regulated in the compound exposure group. The results of GO enrichment analysis and KEGG enrichment analysis of the differentially expressed proteins showed that the compound exposure harmed the spleen antigen recognition, processing, and presentation, inhibited the activation and proliferation of B cells and T cells, and hindered the adaptive immune responses. Our results showed that MWCNTs and DBP compounds can damage the spleen, and impair the innate and adaptive immune functions of the body.
研究领域:
环境毒理学
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免疫学
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蛋白质组学
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分子生物学
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DOI
PubMed
高影响因子
REPRODUCTIVE TOXICOLOGY
·
2025
·
IF 2.8
Huan Li,Hongyan Wang,Xiaolei Xu,Li Qu,Xiuling Sun,Jing Zhang
This study aimed to investigate the protective mechanism of Ginsenoside Rg3 (Rg3) against Di-n-butyl phthalate (DBP) induced spermatogenic damage, focusing on the Src/PI3K/Akt pathway. In vivo experiments demonstrated that Rg3 restored DBP-induced dysregulation of gap junction (GJ) protein connexin 43 (Cx43), improved testicular structure, enhanced sperm parameters (count and motility), and upregulated phosphorylation of Src, PI3K, and Akt (p-Src, p-PI3K, p-Akt) in mice. In vitro studies, using the metabolite of DBP, monobutyl phthalate (MBP), and pathway inhibitors (PP2 for Src and LY294002 for PI3K), further confirmed these effects. Rg3 increased antioxidant enzyme activity, reduced oxidative stress marker MDA, and enhanced the expression of p-Src, p-PI3K, p-Akt, and Cx43 in MBP-treated TM4 Sertoli cells. Inhibitor experiments confirmed that the Src/PI3K pathway mediates Rg3’s protective action. These findings suggest that Rg3 alleviates DBP/MBP-induced male reproductive dysfunction by enhancing antioxidant capacity and reactivating the Src/PI3K/Akt signaling pathway, which helps restore blood-testis barrier (BTB) integrity through Cx43 stabilization. Thus, Rg3 may serve as a potential therapeutic agent for environmental toxicant-related male infertility.
研究领域:
药理学
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毒理学
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生殖生物学
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细胞信号传导
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环境卫生
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 10
·919
·
IF 5.4
Wenhui Li,Xuecheng Zhang,Haojie Zhang,Cheng Zhang,Yingjie Chen,Cong Li,Yonghong Hu,Xiaoping Yu,Biao Zhang,Xiaodong Lin
Plasticizers are a type of toxic substance that may remain in food, posing significant health risks including carcinogenic, teratogenic, mutagenic, and other adverse effects. In this study, a novel strategy was employed by combining Pt@Au nanozymes with high catalytic properties to created two catalytic signal probes, designated as Pt@Au@Ab1 and Pt@Au@Ab2, specifically designed for the detection of dimethyl phthalate (DMP) and dibutyl phthalate (DBP). These catalytic signal probes served as the foundation for the development of a colorimetric immunoassay, enabling the simultaneous detection of both DMP and DBP. The colorimetric immunoassay is capable of detecting DMP in the range of 0.5–100 μg/L with a limit of detection as low as 0.1 μg/L and DBP in the range of 1–32 μg/L with a low limit of detection of 0.5 μg/L. The developed immunoassay can be used for the determination of the DMP and DBP in baijiu and plastic bottled drinks. The recovery rate is in the range of 96.4% and 100.5% and the coefficient of variation is between 1.0% and 7.2%. This innovative colorimetric immunoassay offers a robust tool for the simultaneous quantification of DMP and DBP in real samples.Keywords:colorimetric immunoassay;dimethyl phthalate (DMP);dibutyl phthalate (DBP);Pt@Au nanozyme;two-component simultaneous detection
研究领域:
环境科学
·
分析化学
·
食品安全检测
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产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Haiyue Chen,Yingjia Wang,Hongwu Liang
Dibutyl phthalate (DBP) is a commonly used plasticizer that is frequently detected in water samples due to its widespread use. Titanium dioxide nanoparticles (n-TiO 2 ) have been found to enhance the harmful effects of organic contaminants by increasing their bioavailability in aquatic environments. However, the combined toxic effects of DBP and n-TiO 2 on aquatic organisms remain unclear. This study aimed to investigate the neurotoxicity of DBP and n-TiO 2 synergistic exposure during the early life stage of zebrafish. The results of the study revealed that co-exposure of DBP and n-TiO 2 led to an increase in deformities and a significant reduction in the active duration of zebrafish larvae. Furthermore, the co-exposure of DBP and n-TiO 2 resulted in elevated levels of oxidative stress and altered gene expression related to neurodevelopment and apoptosis. Notably, n-TiO 2 exacerbated the oxidative damage and apoptosis induced by DBP alone exposure. Additionally, co-exposure of the 1.0 mg/L DBP and n-TiO 2 significantly affected the expression of genes associated with neurodevelopment. Moreover, disturbances in amino acid metabolism and interference with lipid metabolism were observed as a result of DBP and n-TiO 2 co-exposure. In general, n-TiO 2 aggravated the neurotoxicity of DBP in the early life stage of zebrafish by increasing oxidative stress, apoptosis, and disrupting amino acid synthesis and lipid metabolism. Therefore, it is essential to consider the potential risks caused by DBP and nanomaterials co-existence in the aquatic environment.
研究领域:
环境毒理学
·
水生生物学
·
分子生物学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Feilong Dong,Qiufeng Lin,Cong Li,Liping Wang,Andreina García
The disinfection of algal-impacted surface waters results in high concentrations of disinfection byproducts (DBPs). Here, the formation of DBPs when water containing Pseudanabaena limnetica ( P. limnetica ) was disinfected combining chlorination and ultraviolet irradiation (UV) was investigated. Because of UV/chlorination to inhibit algal growth and reduce DBP formation effectively, the effects of UV doses, free chlorine doses, reaction time and pH on the formation of DBPs during UV/chlorination were studied. The results showed that higher UV dose, longer oxidation time, neutral and acidic pH were beneficial in controlling DBPs. Moreover, UV/chlorination could enhance the formation of brominated DBPs, and reduce the formation of chlorinated DBPs in algal solutions containing bromide. In addition, the DBP formation from intracellular and extracellular organic matter (IOM and EOM, respectively) derived from algae was compared. Finally, the economic efficiency of our system using the electrical energy per order (EE/O) was evaluated, which was calculated to be 18.68 kWh/m 3 during the reaction. This information indicated that UV/chlorination was a feasible treatment method for algal-rich water.
研究领域:
环境科学
·
水处理
·
消毒工艺
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产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2018
·
IF 4.005
Yu-Hong Huang,Xue-Jing Huang,Xiao-Hong Chen,Quan-Ying Cai,Shaohua Chen,Ce-Hui Mo,Huixiong Lü,Ming-Hung Wong
Di-butyl phthalate (DBP) is a widely used plasticizer , recalcitrant and hazardous organic compound with high detection frequencies and concentrations in water and soil that pose a great threat to human health. A novel endphytic bacterium strain N-1 capable of efficiently degrading DBP and utilizing it as sole carbon source was isolated from Ageratum conyzoides. This bacterium was identified as Bacillus subtilis based on its morphological characteristics and 16S rDNA sequence analysis. Under the optimal culture conditions (pH 7.0, 30 °C), degradation percentage of DBP (12.5–100 mg/L) was up to 95% within five days, and its biodegradation half-life was less than 7.23 h. Degradation percentage of high DBP concentration (200 mg/L) was relatively lower (89%) with half-life of 56.8 h. DBP was degraded by Bacillus subtilis N-1 into mono-butyl phthalate and phthalic acid as evidenced by GC-MS analysis. Bioaugmentation of Youngia japonica plant slurry with strain N-1 greatly accelerated DBP dissipation with 97.5% removal percentage (higher by 47% than non-inoculation). The results highlighted that strain N-1 has great potential for bioremediation by plant-endophyte partnerships and for lowering PAE accumulation in crops.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Ting-Kai Zhu,Pei-Pei Du,Li-Juan Zeng,Huixiong Lü,Hai-Ming Zhao,Yan-Wen Li,Ce-Hui Mo,Quan-Ying Cai
Crops can take up and accumulate di- n -butyl phthalate (DBP), an extensively used plasticizer with endocrine disrupting effect, which poses potential risk to human health. Our previous study found the genotype variation in accumulation of DBP by different cultivars of rice ( Oryza sativa L.). Nevertheless, the effect of DBP metabolism in vivo on the accumulation variation among different plant cultivars remains unknown. In this study, metabolism variation of DBP by low ( Fengyousimiao ) and high ( Peizataifeng ) DBP-accumulating cultivars of rice and the key enzymes involving in DBP metabolism in rice plants were investigated using in vivo exposure of rice plants and in vitro exposure of root crude enzyme extracts. Both mono- n -butyl phthalate (MBP) and phthalic acid (PA) were detected as DBP metabolites in all rice tissues ( i.e. , roots, stems, leaves) and crude enzyme extracts with MBP predominance. DBP metabolism occurred simultaneously when DBP uptake with the highest metabolism in roots in vivo . Degradation of DBP in root crude enzyme extracts fitted well with the first order kinetics ( R 2 = 0.49–0.76, P < 0.05). The activity of carboxylesterase (CXE) in root crude enzyme extracts was significantly positively correlated with DBP degradation rates. CXE played an important role in DBP metabolism of rice plants, confirming by the fact that triphenyl phosphate of CXE inhibitor could inhibit DBP metabolism of in vivo and in vitro exposure. This result was further confirmed by in vitro degradation of DBP with the commercial pure CXE. The crude enzyme solution from roots of Fengyousimiao with higher CXE activity had significantly higher DBP degradation rates than that of Peizataifeng . However, Fengyousimiao with lower tolerance to DBP stress and higher inhibition by triphenyl phosphate displayed lower DBP metabolism ability in vivo than Peizataifeng .
研究领域:
植物学
·
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）