Frontiers in Plant Science
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2023
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IF 5.6
Guohui Li,Muhammad Aamir Manzoor,Guoyu Wang,Cunwu Chen,Cheng Song
Introduction KNOX plays a pivotal role in governing plant growth, development, and responses to diverse abiotic and biotic stresses. However, information on the relationship between the KNOX gene family and expression levels under different treatments in Dendrobium is still limited. Methods To address this problem, we first used bioinformatics methods and revealed the presence of 19 KNOX genes distributed among 13 chromosomes in the Dendrobium huoshanense genome. Through an analysis of phylogenetic relationships, these genes were classified into three distinct clades: class I, class II, and class M. Our investigation included promoter analysis, revealing various cis -acting elements associated with hormones, growth and development, and abiotic stress responses. Additionally, qRT-PCR experiments were conducted to assess the expression patterns of DhKNOX genes under different treatments, including ABA, MeJA, SA, and drought. Results The results demonstrated differential expression of DhKNOX genes in response to these treatments, thereby highlighting their potential roles in stress adaptation. Discussion Overall, our results contribute important insights for further investigations into the functional characterization of the Dendrobium KNOX gene family, shedding light on their roles in plant development and stress responses.
研究领域:
植物学
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分子生物学
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基因组学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Sheng-Xiang Wei,Jie Wang,Qi-Jin Wang,Xiao-Yan Gao,Jun Chen,Ku-Ku Hao,Nai-Dong Chen,Xuan-Xuan Fan,Ya-Feng Dai
One previously undescribed C 22 -diterpenoid alkaloid (DHS-DA-1) was isolated from Dendrobium huoshanense C. Z. Tang et S. J. Cheng. The structure of the new alkaloid was elucidated using extensive spectroscopic analyses and NMR calculations. Griess reaction results showed that the new compound could reduce the creation of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, and the ELISA experiments suggested that DHS-DA-1 could efficaciously decrease LPS-induced inflammation by inhibiting tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expressions. In addition, DHS-DA-1 could effectively inhibit the survival and proliferation of AGS cells and promote their apoptosis in a concentration-dependent manner. The stability and feasibility of binding the DHS-DA-1 to the target protein are further verified by molecular docking and molecular dynamics simulation validation. In addition, DHS-DA-1 exhibits favorable ADMET properties. Graphical Due to the limited space in the graphical abstract, only a portion of the article's content is presented. A diterpenoid alkaloid with 22 carbon atoms was isolated from the stems of Dendrobium huoshanense , exhibiting certain inhibitory effects on the inflammatory factor IL-6 and human gastric adenocarcinoma cells (AGS). The stability and feasibility of the binding between DHS-DA-1 and the target protein (5FUC) were further validated through molecular docking and molecular dynamics simulations.
研究领域:
药理学
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植物化学
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计算生物学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2018
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IF 5.158
Ji-Chun Ge,Xue-Qiang Zha,Chun-Yan Nie,Nian-Jun Yu,Qiang-Ming Li,Dai-Yin Peng,Jun Duan,Li-Hua Pan,Jian-Ping Luo
The present work investigated the inhibitory activity of the polysaccharide from cultivated Dendrobium huoshanense (cDHP) on lung inflammation in cigarette smoke (CS)-induced mouse model. cDHP was mainly composed of mannose and glucose in a molar ratio of 1.89: 1.00, and had a backbone with linkages of 1,4-Man p , 1,4-Glc p , 1,4,6-Man p and 1-Glc p . Hematoxylin and Eosin (HE) staining and immunohistochemistry analysis showed that cDHP can increase alveolar number, thicken alveolar wall, inhibit pulmonary bulla formation and decrease inflammatory cell infiltration as compared to the model group. ELISA determination revealed that cDHP can inhibit CS-induced enhancement in TNF-α and IL-1β secretion in serum and lung. These results suggested that cDHP can resist CS-induced lung inflammation. Further, the phosphorylation analysis of p65, IκB, p38 and JNK as well as the DNA binding activity analysis of NF-κB and AP-1 implied that the anti-inflammation function of cDHP is mediated via regulating NF-κB and MAPK signaling.
研究领域:
药理学
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免疫学
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呼吸病学
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分子生物学
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DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1632
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IF 4.8
Khan Hanif,Zeb Umar,Haider Imran,Rao Weiyi,Wang Chun,Guan Junjie,Alanzi Abdullah R.,Herqash Rashed N.,Abbasov Mehraj A.,Xing Shihai
Extreme environmental conditions have significantly affected the natural habitats of Dendrobium huoshanense , a valuable medicinal orchid highly sensitive to abiotic stress. NAC transcription factors are key plants regulators, contributing to growth, development, and responses to environmental stresses. Despite their importance, information on the NAC gene family in D. huoshanense remains limited. In this study, a genome-wide analysis was conducted to identify and characterize D. huoshanense NAC genes, using integrated bioinformatics, phylogenetic, and expression profiles. A total of 77 DhNAC genes were identified, unevenly distributed across 19 chromosomes, with chromosome 4 containing the highest number of 16 genes. Phylogenetic and structural analysis grouped the DhNAC genes into 20 groups, with conserved NAM domains and diverse exon–intron structures, indicating both evolutionary conservation and functional specialization. Promoter region analysis identified the presence of diverse stress-responsive cis-regulatory elements, such as drought, cold, abscisic acid, methyl jasmonate, and salicylic acid signaling pathways, indicating their roles in stress adaptation. Comparative genomic studies with Arabidopsis thaliana , Dendrobium nobile , Oryza sativa , and Zea mays highlighted evolutionary relationships and revealed significant gene duplication, including tandem and segmental events. Finally, expression analysis of selected DhNAC gene using qRT-PCR showed differential expression under cold stress, methyl jasmonate, abscisic acid, and salicylic acid treatment. Under stress treatments, DhNAC genes showed distinct temporal expression patterns, with ABA inducing early, intermediate, and late responders, MeJA driving strong early-to-mid responses, and SA promoting sustained late responses. Key genes including DhNAC9 , DhNAC19 , DhNAC48 , DhNAC51 , DhNAC53 , DhNAC55 , DhNAC58 , and DhNAC73 were consistently responsive, highlighting their central roles in stress adaptation. These findings provide valuable insights into the role of NAC genes in D. huoshanense in response to environmental stresses.
研究领域:
植物学
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分子生物学
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遗传学
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植物逆境生理学
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生物信息学
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DOI
PubMed
高影响因子
Bioactive Carbohydrates and Dietary Fibre
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2023
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IF 0
Yuhui Pan,Mengnan Wang,Zhiqiang Wang,Xiaojun Huang,Xiaobo Hu,Qin Wang,Shaoping Nie
Dendrobium huoshanense polysaccharide possesses a variety of biologic activities, but their high viscosity leads to poor water solubility which limit their industrialized production and practical application. In this study, two granulation techniques, spray drying and wet granulation were used to prepare the powders containing high Dendrobium huoshanense polysaccharide. The yield, polysaccharides content and solubility were investigated. The results showed that the powders containing high Dendrobium huoshanense polysaccharide prepared by wet granulation (DHP-WG) were more suitable for industrialized production and practical application. The optimal product was DHP-WG-70 with a granulation yield of 78.54 ± 1.02%, polysaccharides content of 70.85 ± 1.67% and dissolution time of 60.05 ± 5.02s. The hypoglycemic activity of DHP-WG and DHP was compared by in vitro experiments including inhibition assay for α-glucosidase, kinetics of inhibition on α-glucosidase, glucose adsorption capacity, glucose dialysis retardation index and inhibition assay for α-amylase activity. In general, DHP had better hypoglycemic activity than DHP-WG. In DHP-WGs, DHP-WG-50 and DHP-WG-70 showed better hypoglycemic activity than DHP-WG-30 and DHP-WG-90. This study provided a theoretical basis for the development and production of powders with high polysaccharides content of Dendrobium huoshanense and hypoglycemic effect in the food industry.
研究领域:
食品工业
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药用植物学
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生物活性
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药物制剂
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DOI
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
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IF 3.1
Baojie Zhu,Cunwu Chen,Jiaxian He,Shaoping Li,Jing Zhao
The interpretation of the chemical quality differences during Dendrobium huoshanense ( D. huoshanense ) traditional processing into ‘Fengdou’ is critical for scientifically evaluating the medicinal value, optimizing the processing methods, and ensuring the stability and predictability of its efficacy. In this study, the quality differences of polysaccharides during the processing of D. huoshanense were conducted utilizing high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), nuclear magnetic resonance (NMR) and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and high-performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD). The results showed that the concentration of non-starch D. huoshanense polysaccharides (NDHP) rose and exhibited greater stability during the processing. The molecular weight (Mw), molecular weight distribution, cumulative molar mass, radius of gyration (Rz), and monosaccharide composition molar ratio of NDHP did not have significant changes during the processing, and these parameters would be affected by the presence of starch. The NMR investigation indicated that the primary glycosidic linkages of β -1,4-Man p and β -1,4-Glc p , together with the acetyl group substitutions at O-2 and O-3 of 1,4-linked Man p , were unaltered. Additionally, the PACE and HPAEC-PAD profiles of acidic and enzymatic hydrolysates of NDHP were similar. These findings contribute to a deeper understanding of the changes in bioactive polysaccharides during the processing of D. huoshanense into ‘Fengdou’, providing theoretical support for improving its processing techniques.
研究领域:
中药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
NATURAL PRODUCT RESEARCH
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2017
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IF 1.828
Hua-Yang Si,Nai-Fu Chen,Nai-Dong Chen,Cheng Huang,Jun Li,Hui Wang
A water-soluble polysaccharide TC-DHPA4 with a molecular weight of 8.0 × 105 Da was isolated from tissue-cultured Dendrobium huoshanense by anion exchange and gel permeation chromatography. Monosaccharide analysis revealed that the homogeneous polysaccharide was made up of rhamnose, arabinose, mannose, glucose, galactose and glucuronic acid with a molar ratio of 1.28:1:1.67:4.71:10.43:1.42. The sugar residue sequence analysis based on the GC-MS files and NMR spectra indicated that the backbone of TC-DHPA4 consisted of the repeated units:→6)-β-Galp-(1→6)-β-Galp-(1→4)-β-GlcpA-(1→6)-β-Glcp-(1→6)-β-Glcp-(→. The sugar residue sequences β-Glcp-(1→)-α-Rhap-(1→3)-β-Galp-(1→, β-Glcp-(1→4)-α-Rhap-(1→3)-β-Galp-(1→, β-Galp-(1→6)-β-Manp-(1→3)-β-Galp-(1→, and α-l-Araf-(1→2)-β-Manp-(1→3)-β-Galp-(1→ were identified as the branches attached to the C-3 position of (1→6)-linked galactose in the backbone.
研究领域:
药用植物学
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化学
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植物学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 16
·5284
·
IF 4.927
Peipei Yang,Mengya Lu,Jing Zhao,Emelda Rosseleena Rohani,Rongchun Han,Nianjun Yu
By applying the hydrophilic ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4mim]Cl), and inorganic salts (K3PO4), an ionic liquid aqueous two-phase system (ILATPS) was established for the separation ofDendrobium huoshanensepolysaccharides (DhPs) and proteins. The effects of inorganic salt concentration, IL quantity, crude DhPs concentration, pH value and temperature were studied to achieve the optimal condition. With the best combination of ILATPS (1.75 g K3PO4, 1.25 g [C4mim]Cl, 10 mg crude DhPs and 5.0 mL ddH2O at pH 7.0 under 25 °C), the extraction efficiency rates for DhPs and proteins were 93.4% and 90.2%, respectively. The processed DhPs retrieved from the lower salt-rich phase comprised mannose, glucose, galactose, arabinose, and galacturonic acid with a molar ratio of 185:71:1.5:1:1 and the molecular weight was 2.14 × 105Da. This approach is fast, simple and environmentally friendly. It provides a new insight into purifying functional polysaccharides of plant origin.Keywords:Dendrobium huoshanense;ionic liquid;polysaccharides;aqueous two-phase system
研究领域:
化学工程
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生物技术
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药学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
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IF 1.5
Jun Dai,Peipei Wei,Yujuan Wang
The addition of heteroatoms changes the structure and optical properties of carbon quantum dots (CQDs). In the paper, nitrogen (N)-doped CQDs are prepared via one-step hydrothermal method from Auricularia auricular and ethylenediamine. Transmission Electron Microscope (TEM) analysis reveals that the average size of carbon quantum dots is 5±2 nm, whereas the chemical study shows the existence of C-N bond. The produced N-doped CQDs have fluorescence quantum yields of 28.4%. Moreover, the prepared CQDs showed high sensitivity and accuracy and had a good proportional band of 0-50 μM for detection Hg 2+ (R 2 =0.9919) and Cd 2+ (R 2 =0.9964). The low detection limits were 77.21 nM and 101.55 nM for Hg 2+ and Cd 2+ , respectively. The CQDs was triumphantly used to track metal ions in environmental samples. So, it was a promising method for environmental pollutant detection.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Qiang-Ming Li,Qiao-Qiao Zhang-Yang,Ya-Yi Tan,Xiang-Ling Wu,Xue-Qiang Zha,Zhen-Zi Shang,Jian-Ping Luo,Feng-Yun Zhang
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder. The stem of Dendrobium huoshanense is a traditional medicinal food. This study aimed to investigate the effect of cultivated D. huoshanense stem polysaccharide (cDHPS) on AD and elucidate its mechanisms using an Aβ 1–42 -induced AD mouse model. Results showed that cDHPS significantly improved the cognitive dysfunction and ameliorated the hippocampal neuronal injury of AD mice. Meanwhile, cDHPS effectively alleviated the neuroinflammation of AD mice not only by decreasing the expression of caspase-1, NOD-like receptor family pyrin domain-containing 3 (NLRP3), and apoptosis-associated speck-like protein containing a CARD (ASC), as well as their downstream inflammatory cytokines, but also by inhibiting the activation of microglia and astrocytes. Furthermore, the levels of glucagon-like peptide-1 (GLP-1) in the hippocampus, plasma, and intestine of AD mice were markedly elevated by cDHPS, along with an increase in intestinal short-chain fatty acids (SCFAs) levels. However, the treatment of exendin 9–39, a GLP-1 receptor antagonist, weakened the protective effect of cDHPS against AD, and the depletion of SCFAs reversed the beneficial effects of cDHPS on cognitive dysfunction, hippocampal neuronal injury, neuroinflammation, and GLP-1 secretion in AD mice. These results suggest that cDHPS could protect against AD by modulating the SCFAs/GLP-1 axis-mediated neuroinflammation suppression.
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产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Shao-Jun Zheng,Xiao-Yan Gao,Xiao-Han Diao,Nai-Dong Chen
Background Cardiovascular diseases, particularly atherosclerosis (AS), remain leading causes of mortality, with limited effective treatments available. Dendrobium huoshanense , a traditional medicinal herb, has shown promising anti-inflammatory and antioxidant effects, but its cardiovascular protective potential remains underexplored. Purpose This study aimed to explore the protective effects of Dendrobium huoshanense polysaccharides (DHP) against AS and elucidate the underlying mechanisms involved. Methods An ApoE(−/−) mice model of AS was established, and DHP was administered at different concentrations via gavage. After 14 weeks, serum and fecal samples were collected. The effects of DHP on lipid profiles, aortic plaques, matrix metalloproteinases (MMP-2 and MMP-9), and the Nrf2/HO-1 pathway were assessed. Additionally, metagenomic sequencing of fecal samples and untargeted metabolomics of serum were conducted and correlations between these findings were explored. Results DHP improved lipid profiles, reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and enhanced superoxide dismutase (SOD) activity. It stabilized aortic plaques, suppressed MMP-2 and MMP-9 overexpression, and activated the Nrf2/HO-1 pathway. DHP also promoted gut microbiota balance, increasing Mucispirillum, Bifidobacterium , and Faecalibaculum while decreasing Desulfovibrionaceae and Eubacterium . Metabolomics revealed alterations in metabolites, including taurochenodeoxycholic acid and ursocolic acid, influencing amino acid, glycerophospholipid, and bile acid metabolism. Conclusions DHP effectively lowers lipid levels, stabilizes aortic plaques, restores gut microbiota balance, and corrects metabolic disturbances, thereby inhibiting the progression of atherosclerosis. These findings provide a scientific basis for the clinical use of DHP in AS prevention and treatment.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Jing-Wen Hao,Nai-Dong Chen,Cun-Wu Chen,Fu-Cheng Zhu,De-Liang Qiao,Yong-Jun Zang,Jun Dai,Xiang-Wen Song,Han Chen
A rapid, green, low cost and nondestructive attenuated total reflection near infrared (ATR NIR) method was developed to quantify the total polysaccharide and the main monosaccharides mannose and glucose in Dendrobium huoshanense. Total 100 D. huoshanense samples from different places were analyzed using ATR NIR method. Potential outlying samples were initially removed from the collected NIR data using the PCA-Mahalanobis distance method. Spectral data preprocessing was studied in the construction of a partial least squares (PLS) model and six different signal pretreatment methods, including multiplicative scattering correction (MSC), standard normal transformation (SNV), first and second derivatives, the combination of MSC with the first derivative, and the combination of SNV with the first derivative, were compared. The results showed that the best signal pretreatment method was the spectral data pretreated by SNV combined with the first derivative due to it showed the lowest root-mean-square error of cross-validation (RMSECV), highest R 2 for both the polysaccharide and its main monosaccharides . In order to improve the performance of the model, the pretreated full spectrum was calculated by different wavelength selection method. The results showed that the optional wavelength selection model was the one simultaneously selecting the NIR wavelength ranges 7500–5750 cm −1 , 5250–4700 cm −1 , 4450–4300 cm −1 and 4200–4100 cm −1 because of the lowest RMSECV and the highest R 2 among the ten wavelength selection models. The external validation and the complete external validation confirmed the robustness and reliability of the developed NIR model. The contents of the total polysaccharide and the main monosaccharides are the essential quality assessment criterion for plant medicines while their traditional quantification methods involved sample destruction, tedious sample processing and non-environmentally friendly pretreatment, therefore, our study might provide an efficient technique tool for the rapid, green and nondestructive quantification of the total polysaccharide and the main monosaccharides for D. huoshanense and other rich-in-polysaccharide plant medicines.
研究领域:
植物学
·
分析化学
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药学
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DOI
高影响因子
危险品化学品经营许可证（带存储）