ENVIRONMENTAL TECHNOLOGY
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2021
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IF 3.247
Yangyang Wang,Qiang Ren,Wenhao Zhan,Kaixuan Zheng,Qi Liao,Zhihui Yang,Yansong Wang,Xinling Ruan
A previous isolated Gordonia sp. (Lff) was used to degrade di-n-octyl phthalate (DOP) contamination in both aqueous solution and soil. The influence of temperature, pH, inoculum size, salt content and initial concentration of DOP on DOP degradation by Lff were analysed. The response of soil bacterial community to DOP and Lff was also analysed by Illumina MiSeq sequence method. Results showed that the optimal temperature, pH, inoculum size and salt content were 35oC, 8.0, 5% and <5%, respectively. Under the optimal condition, more than 91.25% of DOP with different initial concentrations (100–2000 mg/L) could be degraded by Lff. Kinetics analysis indicated that biodegradation of DOP by Lff could be described by first-order kinetics (R2 > 0.917) with the half-life (t1/2) changing irregularly between 0.58 and 0.83 d. In addition, Lff enhanced the removal of DOP in soil and alleviated the toxicity of DOP on soil microorganisms. Furthermore, its influence on soil bacterial community is not obvious. These results suggested that Lff was effective in remediating DOP contamination in different environments.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
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2025
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IF 3.3
Zhuang Haoran,Chu Yiwen,Wang Menglin,Liu Bo,Chen Bihua,Lin Ziyu,Li Zhenxia
The mechanisms underlying phytohormonal disruptions caused by microplastic/plasticizer contamination in agricultural systems remain poorly understood. This study systematically investigates how polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC), and di- n -octyl phthalate (DOP) alter endogenous hormone networks in agricultural systems. Integrated transcriptomic-metabolomic analyses were conducted on cucumber plants grown in hydroponic and soil systems exposed to microplastics (PE, PS, PVC) and DOP. Hormonal pathways were deciphered via gene expression profiling of key biosynthesis regulators. Microplastics and DOP primarily affected the tryptophan-dependent auxin biosynthesis pathway. Differentially expressed genes (DEGs) related to cytokinin biosynthesis were involved in the de novo synthesis pathway. All treatments primarily regulated the expression of DEGs encoding the enzymes gibberellin-20-oxidase (GA20ox), gibberellin-2-oxidase (GA2ox), and gibberellin-3-oxidase (GA3ox) to maintain the balance of active gibberellins. Changes in abscisic acid metabolite levels were linked to the expression of DEGs for abscisic aldehyde oxidase (ABA2). All treatments reduced the ethylene biosynthesis rate. Cucumber plants regulate the expression of DEGs related to phytochrome B (PHYB)-activated inhibitor 1 (CYP734A1) to maintain a balance of endogenous brassinosteroids. Jasmonic acid levels significantly increased after PE, PS, and PS + DOP treatments. Salicylic acid pathways remained unaffected despite phenylalanine ammonia lyase gene variations. PE and PS exhibit significantly stronger impacts on endogenous hormone biosynthesis in cucumber plants compared with PVC. PS with the co-presence of DOP synergistically enhancing jasmonic acid biosynthesis. Notably, smaller PS particle sizes facilitate their absorption by cucumber roots while simultaneously suppressing auxin synthesis efficiency.
研究领域:
植物科学
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环境毒理学
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生物信息学
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农业化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
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2021
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IF 3.247
Yangyang Wang,Wenhao Zhan,Yidan Liu,Shanshan Cheng,Chaosheng Zhang,Jianhua Ma,Runhua Chen
Di-n-octyl phthalate (DOP), a plasticizer used in many different industrial products, is a frequently observed pollutant in the environment. Biodegradation by microorganisms is considered to be a realistic choice for the remediation of DOP contamination. In the present research, the halotolerant bacterial consortium (LF) enriched in our previous research was used to degrade DOP. It was found that the optimal conditions for LF to degrade DOP was temperature 30oC, pH 6.0, inoculum size >5%, and salt content <3%. LF could degrade a high concentration of DOP (2000 mg/L) with the removal efficiency of 96.33%. Substrate inhibition analyses indicated that the inhibition constant, maximum specific degradation rate and half-saturation constant were 2544.6 mg/L, 0.7 d−1 and 59.1 mg/L, respectively. Based on the analysis of the gas chromatography-mass spectrometry (GC-MS), the biodegradation pathway for DOP by LF was proposed. Furthermore, LF could degrade DOP in soil (100 mg/kg) with the highest removal efficiency of 89.3%. This study is the first report on DOP biodegradation by bacterial consortium. These results suggest that LF can be used to remediate DOP-contaminated environment.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Haoran Zhuang,Zhenxia Li,Menglin Wang,Bo Liu,Yiwen Chu,Ziyu Lin
Photosynthesis provides carbon sources and energy for crop growth and development , and the widespread presence of microplastics and plastic plasticisers in agricultural soils affects crop photosynthesis, but the mechanism of the effect is not clear. This study aims to investigate the effects of different microplastics and plasticizers on cucumber photosynthesis. Using polyvinyl chloride (PVC), polyethylene (PE), polystyrene (PS), and di- n -octyl phthalate (DOP) as representative microplastics and plasticizers, we assessed their impact on cucumber photosynthesis. Our results reveal significant alterations in key parameters: intercellular CO 2 concentration (Ci) and transpiration rate (Tr) increased across all treatments, whereas stomatal limit value (Ls) and water use efficiency (WUE) decreased. Notably, PS + DOP treatment led to a significant reduction in the maximum efficiency of photosystem II ( F v / F m ) and ATP accumulation. Furthermore, PE and PS + DOP treatments decreased lycopene and ɛ-carotene synthesis rates, as well as abscisic acid (ABA) accumulation. All treatments inhibited the conversion of β-carotene into strigolactone (SL) and decreased chlorophyll synthesis rates, with PS + DOP exhibiting the most severe impact. Regarding chlorophyll degradation pathways, PVC and PE treatments reduced chlorophyll decomposition rates, whereas DOP with PS promoted degradation. PE and PS treatments also impaired light energy capture, electron transport , and the structural stability of photosystems I and II, as well as photosynthetic capacity and NADPH and ATP synthesis rates. Our findings underscore the differential impacts of microplastics and plasticizers on cucumber photosynthesis, with PS + DOP having the most detrimental effect. These results shed light on the complex interactions between microplastics and plant physiology, highlighting the urgent need for mitigation strategies in agricultural practices to safeguard crop productivity and environmental sustainability.
研究领域:
植物生理学
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环境科学
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农业科学
✓ 已验证
DOI
PubMed
高影响因子
Molecular Catalysis
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2024
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IF 3.9
Shengnan Hu,Ronghui Wu,Ming Guo
Metal-organic framework (MOF)-based immobilized enzymes with green catalytic and reusable features have been widely used in various fields. The pore structure modulation of MOF is the key to the application of MOF materials. The traditional MOF is only microporous, which affects the immobilization rate and catalytic activity of the enzyme. Here, ions that can interact with MOF coordination groups are used to induce the lattice structure of MOF to expand, forming porous carriers with high activity, which not only provides a stable microenvironment for pepsin, but also enables heterogeneous catalysis of pepsin. Pepsin was immobilized onto MOF carriers to form heterogeneous pepsin using immobilized enzyme technology, and the results showed that the immobilization rate of pepsin on MOF carriers modified with pore structure was increased from 49.25 % to 84.68 %. The prepared heterogeneous pepsin showed excellent removal performance of microplastic additive di-n-octyl phthalate (DNOP) contaminant with a removal rate of 95.68 % in 180 min, which is a completely innovative method for the removal of DNOP. In addition, the molecular models of heterogeneous pepsin and DNOP were established, the theoretical results were in agreement with the experimental results, and the degradation pathway of DNOP was analyzed by gas chromatography-mass spectrometry (GC–MS) technique. Our results demonstrate the potential of heterogeneous pepsin as a green catalyst, which can provide new ideas for the construction of heterogeneous enzyme catalysts and the removal of organic pollutants in the future.
研究领域:
催化
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环境科学
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材料科学
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生物技术
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产品关联
DOI
高影响因子
Materials Today Nano
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2026
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IF 7.6
Liangyun Yu,Lingling Huang,Rui Na,Meng Yuan,Zhaoyong Luo,Qi Zhang,Baocai Xu,Qin Xu
Developed crystal phase-tunable Cu-TiO 2 @g-C 3 N 4 with excellent photoelectric property. • Synergistic MIP-heterojunction system enabled efficient recognition of DOP. • The DOP sensor exhibited a very wide linear range and a femtomolar detection limit. • The new sensor has been successfully applied to detect DOP in the real samples. Di-n-octyl phthalate (DOP), a pervasive endocrine-disrupting contaminant in food packaging, plastic products, and aquatic systems, poses severe threats to ecosystem stability and human health. This work proposed a novel molecularly imprinted polymer (MIP)-integrated photoelectrochemical (PEC) sensing strategy for ultra-selective detection of DOP based on a rationally designed crystal phase tunable Cu 2+ -doped TiO 2 @g-C 3 N 4 composite. By lattice-doping TiO 2 with Cu 2+ to generate impurity energy levels, it was possible to precisely tune its band structure and narrow its bandgap. Subsequently, the obtained Cu-TiO 2 was hybridized with two-dimensional g-C 3 N 4 through energy-level matching, forming a tightly coupled structure at the heterojunction, which had strong interactions and thus opened up directional charge separation and transport channels. The precisely controlled heterogeneous and interfacial properties of the Cu-TiO 2 @g-C 3 N 4 matrix were confirmed through characterization methods such as SEM, TEM, HRTEM, XPS and XRD. The fabricated sensor achieved an extremely wide linear detection range of 10 fM to 1 nM, with an ultra-low limit of detection down to 5.30 fM, alongside exceptional anti-interference capability and practical reliability in real sample assays. This study delivered a low-cost, easily miniaturized PEC-MIP platform for trace DOP monitoring, opening up a new high-performance approach for on-site food safety screening and environmental water quality assessment.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）