SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Xiaoxia Liang,Jiakang Duan,Yu Tian,Yue Han,Zewan Qin,Huixia Zhu,Yating Lu,Dongyu Nie,Yingna Guo,Xia Yang
AgCl/Ag 0 shows great promise for the removal of environmental pollutants, while its photocatalytic performance is still a great challenge on account of the agglomeration. Herein, orientational deposited and octahedral AgCl/Ag 0 were obtained by etching the UiO-66-NH 2 template for the first time. The 0.35:1–AgCl/Ag 0 exhibited outstanding efficiency in the degradation of Diazinon (DZN), which was closely 300 times than that of UiO-66-NH 2 and 0.35:1–AgCl@Ag 0 prepared without a template. Its bigger surface area provided more active sites to promote mass transfer efficiency and large amounts of •OH and 1 O 2 were fabricated to offense specific positions of DZN determined through Fukui function. Meanwhile, a high removal rate of 91.0 % was obtained after 5 recycles. Finally, the results of HPLC-MS, T.E.S.T., TOC and IC indicated all intermediates had less toxicity than DZN and were rapidly mineralized. Our work developed a new strategy for designing superb performance AgCl/Ag 0 photocatalysts.
研究领域:
环境科学
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材料科学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
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IF 5.6
Wenhao Zhang,Zhichong Qi,Pengcheng Yan,Ruya Ma,Erjin Zhang,Song Zhang,Keqiang Xu,Li Xu,Henan Li
As a widely used insecticide, diazinon (DIA) is easily hydrolyzed into highly toxic tetraethyl thiophosphate in water, posing significant long-term risks to both aquatic ecosystems and public health. A sensitive photoelectrochemical (PEC) aptasensor was developed by integrating Mo 2 N nanoparticles with g-C 3 N 4 (Mo 2 N/g-C 3 N 4 ) to function as an electron acceptor for DIA detection. The combination of high conductivity and wide spectral response in Mo 2 N enables enhanced photogenerated charge collection. Driven by an internal electric field (IEF), recombination with g-C 3 N 4 can form Schottky junctions, promote directional transfer of electrons, and substantially inhibit electron-hole pair recombination. Thus, g-C 3 N 4 exhibited significantly boosted photocurrent under visible light. Through π-π conjugation effect, an aptamer specifically recognizing DIA was introduced at the electrode interface, establishing the PEC sensing interface. The PEC aptasensor constructed based on this achieves highly sensitive and specific detection of DIA. The sensor showed a broad linear range (5 ×10 −3 -1 ×10 3 pM) and an ultra-low detection limit (1.67 ×10 −3 pM) for DIA. Moreover, it exhibited remarkable accuracy, selectivity, and applicability when applied to real water samples. This work extends the application of Schottky junctions to the sensitive detection of pesticide residues and achieves effective amplification of sensing signals, providing profound insights for constructing excellent PEC aptasensors.
研究领域:
分析化学
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环境毒理学
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生物传感器
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农药残留检测
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光电器件
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分子识别
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DOI
PubMed
高影响因子
Analytical Methods
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2021
·Vol. 13
·期 16
·1911-1918
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IF 2.896
Hui-Ling Wu,Bing-Zhi Wang,Yu Wang,Zhi-Li Xiao,Lin Luo,Zi-Jian Chen,Yu-Dong Shen,Zhen-Lin Xu
Diazinon (DAZ) is an organophosphorus pesticide (OP), which is commonly used to prevent and control harmful pests that endanger agricultural products. In this study, we developed a novel heterology coating strategy for the immunoassay of DAZ. The DAZ coating hapten can be directly conjugated to the carrier protein without requiring a spacer arm. This proposed hapten coating strategy is time-saving and significantly improves the sensitivity of the immunoassay due to the lack of a spacer arm. The as-synthesized coating antigen was used to screen the monoclonal antibody (mAb). Finally, the developed indirect competitive enzyme-linked immunoassay (icELISA) showed IC50 and limit of detection (LOD) values of 0.58 ng mL−1 and 8 pg mL−1, respectively. This method exhibited negligible cross-reactivity towards other analogues, and the recoveries of samples (cucumber, cabbage, and lettuce) ranged from 92.6% to 125.4%, with coefficients of variance (CV) below 12%. Good correlation between icELISA and high-performance liquid chromatography mass spectrometry (HPLC-MS/MS) was obtained. The proposed icELISA was an ideal tool for monitoring DAZ residues in food samples.
研究领域:
农业化学
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分析化学
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食品安全
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DOI
PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2021
·
IF 6.158
Boqian Li,Shu Li,Ludong Yi,Haosheng Sun,Jun Qin,Jun Wang,Dawei Fang
Diazinon (DZN), a broad-spectrum and highly effective organophosphorus pesticide, is widely being used in agricultural production and household environments. However, massive DZN effluent can be generated during the DZN production process, which will cause devastating effects on the ecosystem and damage human health due to the improper dispose of pesticide effluent before discharging. In this work, hydrodynamic cavitation (HC), a new advanced oxidation technology, was used to degrade DZN in wastewater for the first time. The influences of DZN initial concentration (5.0–15 mg/L), solution pH (4.0–10), temperature (30–50 °C) and some orifice plate geometric parameters (orifice numbers and orifice half angles) on the degradation effect of DZN were investigated. It can be observed that the DZN in solution can be effectively degraded in orifice plate HC system based on three orifices with 45° convergent half angle and the degradation ratio can reach 50.52 % under the operation conditions of 150 min treatment time, 30 °C temperature and pH = 4.0 acidity. Subsequently, the studies of intensifying DZN degradation were also carried out through adding different inorganic oxidants such as potassium peroxodisulfate (K 2 S 2 O 8 ), potassium periodate (KIO 4 ) and sodium perchlorate (NaClO 4 ). The maximum DZN HC degradation extent (81.92 %) can be achieved in the presence of K 2 S 2 O 8 under the optimized conditions. The generated intermediate products in DZN HC degradation process are some low-toxic and low-active substances. And that, these intermediate products can be further mineralized to H 2 O, CO 2 and inorganic ions. On the basis of cavitational yield, the energy consumption analysis has been performed. Overall, this work demonstrates that the HC combined with oxidant K 2 S 2 O 8 may be a promising strategy for the large-scale treatment of organophosphorus pesticide wastewater.
研究领域:
环境科学
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农药化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
·
IF 3.8
Yang Xiaoying,Liu Qian,Luo Longhui,Tian Wei,Kang Chao,Yang Wanliang,Li Tianxiang,Chen Dongmei,Yan Xiufang
A novel biosensing strategy based on the synergy of aptamer molecular recognition and surface-enhanced Raman spectroscopy (SERS) has been developed to address the growing problem of groundwater and soil contamination by the organophosphorus pesticide diazinon (DZN). By combining a high-affinity aptamer with a plasmonic resonance-enhanced substrate, a SERS biosensing interface with single-molecule detection capability was successfully constructed, and the electrochemical surface-enhanced Raman spectroscopy (EC-SERS) method using AgNPs-modified screen-printed electrodes (SPEs) was also used for sensitive detection of DZN. The results showed that the constructed SERS aptamer sensor was able to specifically identify diazinon in the system where multiple interfering pesticides coexisted, and the detection limit of the sensor for diazinon was up to 5.33 × 10 −10 M. The intensity of the characteristic peaks (602 cm −1 ) showed a good linear response with the concentration of DZN, which demonstrated very high detection sensitivity and specific identification function. The proposed EC-SERS method significantly improves the SERS signals of pesticides by potentiometrically modulating the plasmonic resonance coupling effect at the interface of AgNPs-modified electrodes, inducing an orientationally regularised arrangement of DZN molecules. The SERS aptasensor was used to detect diazinon in wastewater and subsurface soil with good recoveries (83.20%–117.78%), which were in good agreement with the results obtained by high-performance liquid chromatography. The results demonstrated that the SERS aptasensor has good sensitivity, stability, simplicity of operation, and specific identification, which provides a solution with ultra-sensitive response, rapid analysis, and strong anti-interference for the detection of trace pesticide.
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PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Wang Guangli,Li Xiang,Zheng Jiaxin,Li Xuedong,Bai Lingling,Yue Wenlong,Jiang Li
Diazinon is one of the most widely used organophosphate insecticides, one that is frequently detected in the environment. In this study, a diazinon-degrading bacterium, DI-6, previously isolated from diazinon-contaminated soil in China has been subsequently identified as Sphingobium sp. on the basis of its physiological and biochemical characteristics, as well as by virtue of a comparative analysis of 16S rRNA gene sequences. This strain is capable of using diazinon as its sole carbon source for growth and was able to degrade 91.8% of 100 mg L–1 diazinon over a 60-h interval. During the degradation of diazinon, the following seven metabolites were captured and identified by gas chromatography/mass spectrometry (GC–MS) analysis: diazoxon, diazinon aldehyde, isopropenyl derivative of diazinon, hydroxyethyl derivative of diazinon, diazinon methyl ketone, O-[2-(1-hydroxyethyl)-6-methylpyrimidin-4-yl] O-methyl O-hydrogen phosphorothioate, and O-(6-methyl pyrimidin-4-yl) O,O-dihydrogen phosphorothioate. Based on these metabolites, a novel microbial biodegradation pathway of diazinon by Sphingobium sp. DI-6 is proposed. This research provides potentially useful information for the application of the DI-6 strain in bioremediation of diazinon-contaminated environments.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
COMPUTERS IN BIOLOGY AND MEDICINE
·
2024
·
IF 7
Jianan Li,Yan Sun,Haoran Bi
Diazinon (DZN), a persistent organophosphate insecticide, has been associated with neurotoxic effects, particularly in the hippocampus. However, the specific molecular mechanisms of DZN-induced hippocampal toxicity remain unknown. In this study, we analyzed the mRNA and miRNA expression patterns of HT22 cells following exposure to DZN (125 μM), and the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted subsequently. The integration of miRNA sequencing (miRNA-seq) and mRNA sequencing (mRNA-seq) data identified 33 differentially expressed miRNAs (DEMIs, 15 up-regulated and 18 down-regulated) and 271 differentially expressed mRNAs (DEMs, 69 up-regulated and 202 down-regulated) targeted by the DEMIs. Moreover, the 3 most central mRNAs (ITGAV, FN1, and EGFR) and 7 associated miRNAs (mmu-miR-700-5p, mmu-miR-26a-2-3p, mmu-miR-452-3p, mmu-miR-25-3p, mmu-miR-582-5p, mmu-miR-467a-5p, and mmu-miR-467b-5p) were screened and validated using quantitative real-time PCR. Furthermore, the GO analysis revealed that the identified DEMs were enriched in biological adhesion extracellular matrix, and growth factor binding, while the KEGG analysis suggested that the enriched DEMs were involved in ECM-receptor interaction, mTOR signaling pathway, MAPK signaling pathway, and AMPK signaling pathway. Our results may aid in elucidating the underlying mechanisms associated with DZN-induced hippocampal toxicity and provide valuable insights into the pathogenesis of neurotoxicity triggered by other organophosphorus pesticides.
研究领域:
神经毒理学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Mei Zhang,Qianqian Kong,Jingcheng Huang,Yaodong Xiang,Guanjie Wang,Jie Han,Yemin Guo,Shancang Zhao,Xia Sun
Within this work, an electrochemiluminescence (ECL) aptasensor was constructed for the sensitive detection of diazinon . Primarily, AuNPs not only provided a platform for luminol fixation due to their favorable bioactivity and conductivity , but they facilitated the improvement of ECL signals. Furthermore, three-dimensional (3D) flower-like ZnONPs were obtained by hydrothermal synthesis , which could be an efficacious catalyst for the luminol-hydrogen peroxide (luminol-H 2 O 2 ) system, whereby the ECL signal of the sensor could be amplified. Given the large specific surface area of 3D flower-like ZnONPs, modification of positive charges on their surface could well immobilized the electronegative aptamer chains on the electrode surface , and when diazinon (DZN) was added, the aptamer combined with DZN to form a complex to hinder the ECL intensity, and the detection of DZN was achieved by the change of ECL signal intensity. After evaluated the experimental parameters, the detection limit of DZN was 0.0109 nM (S/N = 3), and the linear range was 0.0328–3.28 × 10 4 nM. The proposed ECL aptasensor had comparatively satisfactory detection limits, which provided a reasonable reference for other contaminant residues detection.
研究领域:
分析化学
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环境科学
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食品安全
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纳米技术
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生物化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Chaojie Yu,Jie Yang,Yuxin Sang,Congmin Liu,Juan Wang,Zuwei Song,Fansheng Cheng,Weina Li
Biomass-derived blue CDs were immobilized in DZN-imprinted chitosan gel beads. • An IFE-governed fluorescence decrease enabled dual-mode readout by fluorometry and standardized smartphone imaging. • Rapid on-site determination was achieved within ∼10 min over 40–2000 μg/L. • Low detection limits were obtained (8.8 nM by fluorometry; 18.7 nM by smartphone readout). • The method showed good selectivity, matrix robustness, and agreement with an LC-MS reference method in real waters. A portable and environmentally benign sensing method was developed for on-site determination of diazinon (DZN) in environmental waters based on blue fluorescent carbon-dot-embedded molecularly imprinted chitosan gel beads (B-CDs@MIPGB). Biomass-derived blue carbon dots (B-CDs) were synthesized from yellow rose extract and immobilized within a DZN-imprinted chitosan network via a one-pot imprinting–crosslinking strategy followed by coagulation-bath bead formation. DZN binding in the imprinted cavities induced a concentration-dependent fluorescence decrease mainly governed by an inner filter effect (IFE), enabling dual-mode quantification by both fluorometry and standardized smartphone imaging. The method provided a linear response from 40 to 2000 μg/L with detection limits of 8.8 ± 0.09 nM (fluorometry) and 18.7 ± 0.12 nM (smartphone readout). A low-cost imaging box with fixed acquisition settings was employed to minimize variability in smartphone analysis, yielding repeatable G/B ratios with RSD below 3.59%. The beads exhibited high selectivity over common coexisting pesticides and metal ions, and maintained reliable performance under representative matrix interferences including humic acid, turbidity, and mixed major ions. Benchmarked against a reference LC-MS method using identical spiked samples, the developed approach achieved recovery rates of 97.2%–104.4% (fluorescence) and 98.4%–104.8% (smartphone), confirming its accuracy. This bead-based platform integrates selective recognition, rapid operation (∼10 min), and field-deployable readout, offering a practical tool for routine environmental surveillance of organophosphorus pesticide residues. One-pot imprinting embeds yellow-rose-derived B-CDs into chitosan gel beads using DZN as a template. After cross-linking and template removal, DZN-selective cavities enable rebinding/enrichment, producing IFE-dominated fluorescence quenching. Dual readout (fluorometer/smartphone) supports on-site analysis, validated in real waters by LC-MS. Download: Download high-res image (259KB) Download: Download full-size image
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Pingyue Wang,Huanhuan Li,Md Mehedi Hassan,Zhiming Guo,Zheng-Zhu Zhang,Quansheng Chen
In this study, a highly sensitive upconversion fluorescence (FL) biosensor was developed for the detection of organophosphorus pesticides (OPs) based on an acetylcholinesterase (AChE) modulated FL “off–on–off” strategy. The luminescence of synthesized UCNPs could be quenched strongly by Cu2+ due to an energy transfer effect. Upon addition of AChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) could seize Cu2+ from UCNPs-Cu2+ mixture, resulting in the quenched FL triggered on. OPs could irreversibly impede the activity of AChE, which caused the formation of thiocholine to decrease, thus, reduced the recovery of FL. Under the optimum conditions, a linear detection range from 0.1 to 50 ng/mL was achieved for the representative OPs (diazinon) with LOD of 0.05 ng/mL. Furthermore, the ability of the biosensor to detect OPs was also confirmed in adulterated environmental and agricultural samples. In validation analysis, the proposed sensor showed satisfactory results (p > 0.05) with GC–MS.
研究领域:
生物传感技术
·
食品安全检测
·
分析化学
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产品关联
DOI
PubMed
高影响因子
NEUROTOXICOLOGY
·
2022
·
IF 4.398
Jianan Li,Haoran Bi
Diazinon (DZN) is a commonly used organophosphorus pesticide that was recently found to cause hippocampal degeneration in rodents. In this study, we elucidated the underlying molecular mechanisms through integrated network pharmacology and in vitro toxicity screening. 37 potential molecular targets of DZN-induced hippocampal neurotoxicity were predicted. Identified targets were then included in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A preliminary protein-protein network (PPI) was constructed using STRING, and the top 10 network hub target genes (Akt1, Mapk3, Tnf , Il6 , Ptgs2 , Il10, Il2, Il4, Creb1 , and Fgf2) were screened for expression changes under DZN treatment. Cell counting kit-8 (CCK8) and lactate dehydrogenase (LDH) assays revealed time- and dose-dependent toxicity of DZN against mouse hippocampus-derived HT22 cells. Acetylcholinesterase (AChE) activity assay suggested that DZN inhibited the AChE activity, and TUNEL staining revealed that DZN increased the apoptotic rate. The mRNA expression levels of 9 hub targets (all except Il10) showed significant changes during DZN treatment, and AChE activity inhibition correlated strongly with Akt1, Mapk3, Il6, Il2, and Fgf2 . DZN-induced hippocampal neurotoxicity was associated with the altered activity of multiple signaling pathways (including PI3K-Akt, TNF, and apoptosis signaling). These results provided a theoretical basis for more precise elucidation of DZN neurotoxic mechanisms.
研究领域:
药理学
·
毒理学
·
分子生物学
·
神经科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Tang,Tingting Cai,Zexi Chen,Na Li,Jintong Liu,Hong Yang
The pervasive application of organophosphorus pesticides (OPs) in agriculture led to a persistent accumulation and causing environmental pollution and safety problems. In response, this study presented a Cu(II)-blocked aggregation-induced emission (AIE)-based metal organic framework (MOF) nanosystem for sensing diazinon. Based on the inhibition capability of diazinon on glutathione peroxidases, the oxidation and consumption of glutathione (GSH) was restricted. Owing to redox reaction between Cu(II) and abundant GSH relived by diazinon, the aggregation-induced emission luminogens (AIEgens) sealed in MOF could be released in aqueous solution and reassembled into emissive aggregates with significantly enhanced signals. The phase transformed-AIE fluorescence recovery provided sensitive diazinon detection method with a detection limit of 0.14 ng mL −1 , which was also reliable in food samples and bioaccumulation monitoring in rice. Thus, this work introduced a highly sensitive AIE-MOF-based fluorescence sensing approach for analyzing diazinon in crops, which was of significant importance for food safety and environmental monitoring.
研究领域:
分析化学
·
环境科学
·
食品科学
·
纳米技术
·
农药检测
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产品关联
DOI
PubMed
高影响因子
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