SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Qilu Cheng,Qifa Zhou,Zhuo Jin,Ying Jiang,Ligen Xu,Hui Jiang,Yuhua Zhao
Algal cultures of Dictyosphaerium sp. were treated with a wide range (0–8 mg/L) of nonylphenol (NP) applications to investigate the species' potential to perform bioremediation of NP-contaminated wastewater and explore the mechanism of NP toxicity in algal cell. The algal species exhibited a high tolerance for NP and efficiently removed even high concentrations of NP. The accumulation of NP in algal cells tended to increase and the percentage of NP adhered to the cell walls tended to decrease as the NP concentration increased. The thylakoid density and the pyrenoid size also tended to decrease as the NP concentration rose. The key genes involved in the antioxidative pathways and photosynthetic pathways were down-regulated in the NP-treated algal cells. Algal ribosome genes were especially sensitive to NP. The results indicated that NP could induce oxidative stress in algal cells and that Dictyosphaerium sp. showed potential as a bioremediation agent.
研究领域:
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Zhuo Jin,Linna Du,Qilu Cheng,Ying Jiang,Cai Hui,Ligen Xu,Yuhua Zhao,Hui Jiang
Microplastics (MPs) and nonylphenol (NP) are typical pollutants that are frequently detected in aquatic environments and can pose a risk to aquatic organisms. However, the responses of algae, the producers in aquatic ecosystems , to MP and NP co-exposure have not been extensively investigated. In this study, polystyrene (PS, 50 mg/L) was selected as a representative MP to evaluate its short-term effects on algae treated with NP (4 mg/L). The results showed that PS mitigated the toxicity of NP to algae after 96 h of exposure, as illustrated by the higher cell densities and pigment concentrations, as well as lower extracellular protein contents and better integrity of intracellular structures, in algae subjected to PS + NP treatment compared with those subjected to NP treatment. Moreover, the upregulated expression of genes involved in photosynthesis and downregulated expression of ribosomal genes as well as genes encoding ATPase and antioxidase, analyzed through RNA-sequencing analysis, further indicated the potential repair and defense mechanisms of PS in NP-treated algae.
研究领域:
环境科学
·
水生生态学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Qilu Cheng,Linna Du,Ligen Xu,Yuhua Zhao,Junwei Ma,Hui Lin
Nonylphenol (NP) toxicity limits the improvements in its algal remediation efficiency. This study comprehensively investigated the performance and mechanism of NaHCO 3 -driving effects on NP-exposed algae. The results showed that NaHCO 3 enhanced algal resistance to NP and the corresponding EC 50 values increased 1.31–4.25 times. Further, the toxicological effects of NP reduced with increasing pyrenoid volume and chlorophyll and carotenoids production, and decreasing cellular damage degree. Moreover, the concentration of extracellular polymeric substances was enhanced and more NP adsorption sites were formed. Consistently, RNA-seq demonstrated significant expression alterations in genes related to energy metabolism, cellular synthesis, photosynthesis, and carbon fixation. Besides, NP biodegradation rate was increased by 15.2 % and 11.1 % in the 1, and 4 mg/L NP treatments, respectively. Identification of degradation intermediates and their toxicity via Ecological Structure Activity Relationship program showed that NaHCO 3 accelerated sequential α-C removal from NP in algae with faster generation of less toxic metabolites, namely, 4-ethylphenol, 4-cresol and 4-hydroxybenzoic acid. This study provides new insights into the role of NaHCO 3 in toxicity alleviation and metabolism enhancement of NP in algae and can assist NP bioremediation efforts in aquatic environment.
研究领域:
环境科学
·
水生毒理学
·
生物修复
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water
·
2022
·Vol. 14
·期 5
·718
·
IF 3.53
Wan Liu,Shan Chen,Han Zhou,Xianyun Wang,Houtao Xu,Liqing Wang,Wei Zhang,Lijing Chen
Sulfonamides (SAs) are the most common and bio-refractory antibiotics detected in surface water systems, which cause long-term toxic effects on aquatic organisms. This study used the combination of a BiVO4photocatalyst and freshwater micro-green alga (Dictyosphaeriumsp.) to remove sulfadiazine (SD) and sulfamethazine (SM2) at an initial concentration of 5 mg/L (1:1v/v) for 7 days. We set up three gradient concentrations of BiVO4(0.5, 1 and 2 g/L) combined with the same concentration (80 mg/L) ofDictyosphaeriumsp. and then prepared corresponding concentrations of pure BiVO4and pure microalgae as controls. We evaluated the ability of BiVO4andDictyosphaeriumsp. combined technology to remove SAs by observing the removal efficiency of antibiotics and explained the degradation mechanism of antibiotics and the key role of microalgae by studying the changes of reactive oxygen species (ROS) and inorganic ions (nitrogen, sulfur). The results showed that the degradation rate of these two SAs in the 0.5 g/L BiVO4–algae group could reach >96% within 7 d, which was higher than that in the 2 g/L BiVO4group (93%) and the algae group (28%). The increased degradation efficiency of SAs in BiVO4and microalgae systems was mainly due to the increased amount of ROS. Meanwhile, more SAs were degraded to inorganic compounds such as NH4+-N, NO3−-N and SO42−-S under ROS stress. It was found that microalgae can absorb the degradation products of antibiotics such as NH4+-N for their own growth, thereby reducing the toxicity of antibiotic by-products. In addition, BiVO4had no damaging effect on the autofluorescence intensity of the microalgae. Our study provides an efficient and eco-economic approach to remove antibiotics using visible-light irradiation in aquatic environments and provides new insights into the biological removal of other antibiotic contaminants in aquatic environments.Keywords:Dictyosphaeriumsp.;BiVO4;sulfamethazine;sulfadiazine;reactive oxygen species;NH4+-N
研究领域:
环境科学
·
水生生态学
·
光催化
·
微生物学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 14
·20711-20720
·
IF 4.223
Jiang Ying,Du Linna,Cheng Qilu,Jin Zhuo,Hui Cai,Zhao Yuhua,Jiang Hui,Xu Ligen
Nanoparticles and organic pollutants are two major contaminants found in aquatic environments. Algae are regarded as the model organism for the risk assessment of pollutants in water. In our previous study, we investigated the toxic effects of nonylphenol (NP), a typical organic water pollutant, on algae; however, it remains unclear how algae respond to the coexistence of NP and nanoparticles. In this study, a concentration gradient of nanoscale zero-valent iron (nZVI; 10, 50, 100, and 200 mg/L) was added to NP-exposed Dictyosphaerium sp. to investigate both the toxic effects of this combination and the potential for NP removal. nZVI had a dose-dependent effect on NP-exposed algae, with high nZVI concentrations significantly decreasing algal biomass and pigment content, as well as severely damaging algal cellular ultrastructure. In addition, genes involved in antioxidant response, photosynthesis, and ribosome synthesis were significantly altered when NP-exposed algae were incubated with nZVI. In contrast to high nZVI concentrations, adding a small concentration of nZVI led to reduced toxicity in NP-exposed algae, while significantly enhancing the NP removal rate. This study improves our understanding of algal responses to various pollutants and suggests that nZVI may assist in the remediation of NP in aquatic ecosystems.
研究领域:
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）