JOURNAL OF APPLIED ENTOMOLOGY
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2022
·Vol. 146
·期 6
·760-772
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IF 2.183
Ya Xing,Fang Niu,Xiao-Mei Wang,Huan-Wen Chen,De-Fu Chi
Dioryctria abietella is a major pest of conifer cones that can initiate specific behaviours (locating hosts, finding mates and selecting oviposition sites) through sensitive olfactory systems. General odorant-binding proteins are involved in the first step in odorant perception in insects, but their specific functional roles in D . abietella chemoreception have not been fully elucidated. In this study, quantitative real-time polymerase chain reaction (PCR) examinations showed that DabiGOBP2 is predominantly expressed in the antennae of female adults. Western blot experiments further confirmed that these proteins show biased expression in the tissues. Binding property analysis demonstrated that DabiGOBP2 exhibited ligand specificity to terpenes, especially myrcene ( K i = 8.19 ± 0.05). Electroantennogram (EAG) and Y-tube assays showed that three plant volatiles elicited high EAG responses, but only dodecanal showed a strong attraction to females. However, myrcene and isobutyl benzoate were significantly repellent to males at high doses in the behaviour test. In conclusion, exploring the functions of DabiGOBP2 can help in better understanding the mechanism of the olfactory system of D . abietella and developing novel strategies to control this pest.
研究领域:
昆虫学
·
分子生物学
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高影响因子
Insects
·
2022
·Vol. 13
·期 12
·1145
·
IF 3.139
Chun Wu,Ningna Yin,Yuruo Guo,Zhengquan Wang,Naiyong Liu
Simple SummaryDioryctria abietella(Lepidoptera: Pyralidae) is a destructive forest pest that feeds on the shoots and cones of Pinaceae plants. The reception of host volatiles and toxic compounds can be mediated via odorant binding proteins (OBPs) expressed in olfactory organs. Here, a number of DabiOBPs may be involved in reproduction, as indicated by the expression profiles. Further, DabiOBP5 and DabiOBP14 enriched in adult antennae could strongly interact with two plant volatiles and four insecticides. Of these, β-ionone derived from plant flowers possibly served as an feeding attractant of female moths, while a pear ester ethyl-(2E,4Z)-decadienoate identified as a non-host volatile may repelD. abietella. In addition, two DabiOBPs were possibly associated with insecticide resistance. Molecular simulations identified several key residues involved in ligand-binding, revealing different binding mechanisms of DabiOBP5 and/or DabiOBP14 with the best ligands. These findings provide potentially active compounds for the control ofD. abietellaand characterize the interaction mechanisms of two antennal DabiOBPs with plant odorants and insecticides.AbstractThe management of forest pests has become a significant challenge, particularly for wood borers, because they spend most of the time in the trunks or cones. The coneworm,Dioryctria abietella, is a representative of cone borers as its larvae feed on the cones of Pinaceae plants. The molecular mechanisms underlying the interactions between this species and host plants or habitats can assist in developing strategies for pest control. In this study, we extended the expression profiles of 32 odorant binding proteins (OBPs) in the reproductive tissues ofD. abietella, revealing the detectable transcription of 29 genes. Using two DabiOBPs highly expressed in antennae (DabiOBP5 and DabiOBP14) as targets, six compounds with high affinities (dissociation constants < 13 μM) were identified through a reverse chemical ecology strategy, including insecticides widely used for the control of lepidopteran pests. Of these compounds, a floral volatile β-ionone and a pear-produced ester ethyl-(2E,4Z)-decadienoate may serve as behaviorally active compounds inD. abietella. The strong binding of DabiOBPs to insecticides suggested their involvement in insecticide resistance, reflecting sophisticated detoxification mechanisms of this moth. In the molecular simulations, DabiOBP14 possessed stronger interactions with the six ligands compared to DabiOBP5, in which a few key residues within the binding pockets were involved in the formation of hydrogen bonds. This study provides some valuable reference active compounds for the development of lures or repellents inD. abietellaand unravels the putative roles of two antenna-dominant DabiOBPs in the perception of plant-derived odorants and insecticides.Keywords:Dioryctria abietella;odorant binding protein;reproduction;ligand-binding assay;plant odorant;insecticide resistance;molecular simulation
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高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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2023
·
IF 2.2
Yu-Ruo Guo,Ning-Na Yin,Chun Wu,Zi-Xuan Yang,Zheng-Quan Wang,Nai-Yong Liu
In the forest ecosystem dominated by the Pinaceae plants, this boring pest Dioryctria abietella is subject to a variety of odorants derived from host and nonhost plants, in which olfactory-related proteins enriched in antennae are key behavioral modulators for the orientation of feeding and ovipositing hosts. Here, we addressed the odorant binding protein (OBP) gene family in D. abietella . Expression profiles revealed that the majority of OBPs were abundantly expressed in the antennae at a female-biased level. A male-antenna-biased DabiPBP1 was a strong candidate for detecting type I and type II pheromones of D. abitella female moths. Using a prokaryotic expression system combined with affinity chromatography, we harvested two antenna-dominant DabiOBPs. In the ligand-binding assays, the two DabiOBPs exhibited different odorant response spectra, as DabiOBP17 was tuned to most odorants with higher affinities compared to DabiOBP4. Of these, DabiOBP4 could strongly bind syringaldehyde and citral (dissociation constants (K i ) < 14 μM). A floral volatile, benzyl benzoate (K i = 4.72 ± 0.20 μM), was the best ligand for DabiOBP17. Remarkably, several green leaf volatiles were found to strongly interact with DabiOBP17 (K i < 8.5 μM), including Z 3-hexenyl acetate, E 2-hexenol, Z 2-hexenal and E 2-hexenal that may mediate a repellent response to D. abietella . Structural analyses of ligands revealed that the binding of the two DabiOBPs to odorants was associated with carbon-chain lengths and functional groups. Molecular simulations identified several key residues involved in the interactions of DabiOBPs and ligands, suggesting specific binding mechanisms. This study highlights olfactory roles of two antennal DabiOBPs in D. abietella , helping the identification of potentially behavioral compounds for the population control of this pest.
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高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
·
IF 4.7
Ning-Na Yin,Yu-Juan Yao,Yin-Lan Liang,Zheng-Quan Wang,Yong-He Li,Nai-Yong Liu
The orientation of the oligophagous cone-feeding moth Dioryctria abietella (Lepidoptera: Pyralidae) to host plants primarily relies on olfactory-related proteins, particularly those candidates highly expressed in antennae. Here, through a combination of expression profile, ligand-binding assay, molecular docking and site-directed mutagenesis strategies, we characterized the chemosensory protein (CSP) gene family in D. abietella . Quantitative real-time PCR (qPCR) analyses revealed the detectable expression of all 22 DabiCSPs in the antennae, of which seven genes were significantly enriched in this tissue. In addition, the majority of the genes (19/22 relatives) had the expression in at least one reproductive tissue. In the interactions of four antenna-dominant DabiCSPs and different chemical classes, DabiCSP1 was broadly tuned to 27 plant-derived odors, three man-made insecticides and one herbicide with high affinities (K i < 6.60 μM). By contrast, three other DabiCSPs (DabiCSP4, CSP6 and CSP17) exhibited a narrow odor binding spectrum, in response to six compounds for each protein. Our mutation analyses combined with molecular docking simulations and binding assays further identified four key residues (Tyr25, Thr26, Ile65 and Val69) in the interactions of DabiCSP1 and ligands, of which binding abilities of this protein to 12, 15, 16 and three compounds were significantly decreased compared to the wildtype protein, respectively. Our study reveals different odor binding spectra of four DabiCSPs enriched in antennae and identifies key residues responsible for the binding of DabiCSP1 and potentially active compounds for the control of this pest.
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高影响因子
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