CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Chun-Qian Zhang,Xia Chen,Kan Ding
Dioscorea opposita Thunb. is widely used as functional foods and traditional Chinese medicine in China for its activity of regulating function of spleen and stomach. Polysaccharides may contribute to the function of regulation. To investigate structure features and bioactivities of polysaccharides from D. opposita, the rhizome of D. opposita was extracted with boiling water, yielding crude polysaccharides DOP. A novel polysaccharide named DOP0.1-S-1 was isolated from DOP by further purification. The average molecular weight of DOP0.1-S-1 was 10,000 Da and the range was around 12,000 –1,200 Da. The carbohydrate content of DOP0.1-S-1 was 100% and no protein was detected. The monosaccharide analysis showed that DOP0.1-S-1 was mostly composed of galactose. Methylation and NMR spectra analysis indicated that DOP0.1-S-1 was a 1,4-β-galactan. Bioactivity test showed that DOP0.1-S-1 could promote the growth of B. thetaiotaomicron and B. ovatus and produce the short-chain fatty acids during the utilization of the polysaccharide.
研究领域:
食品科学
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微生物学
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植物化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Xin Zhang,Lizhu Fu,Yujiao Hou,Nan Long,Guangqiang Zhu,Xiaofang Liao,Lidong Zhou,Jinghua Lu,Weijun Kong
Trace detection of Fe 3+ ions in complex matrices requires the development of highly sensitive sensors. Nitrogen-doped carbon dots (N-CDs) with admirable fluorescence efficiency, high photostability, and good biocompatibility have drawn special interest in fluorescent sensing of metal ions. In this work, a facile and economical microwave-assisted strategy was introduced for rapid synthesis of green fluorescence N-CDs, which were comprehensively characterized to display excitation-dependent fluorescent behavior with a high quantum yield of 18.1%, as well as good water solubility and high selectivity to Fe 3+ ions. Then, a N-CDs based fluorescent “on-off” nanosensor was successfully fabricated for sensitive assay of Fe 3+ ions. In the presence of Fe 3+ ions, fluorescence of N-CDs was remarkably quenched due to the formation of N-CDs/Fe 3+ complex through static quenching mechanism. Under the optimized synthetic and sensing conditions, the newly-developed nanosensor was free from other interfering metal ions for selective detection Fe 3+ ions ranging from 1.0 to 80.0 μM with a low limit of detection of 1.0 μM, which are comparable with other methods. Practical application in Dioscorea opposita Thunb. samples exhibited fascinating recoveries of 98.5%−109.2% with relative standard deviations lower than 4.7%. The N-CDs based fluorescent nanosensor with simple construction, high selectivity and extremely sensitivity contributed a promising tool for rapid and environmental-friendly assay of Fe 3+ ions in a large number of actual samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
JSFA Reports
·
2023
·Vol. 3
·期 3
·137-145
·
IF 0
Ruize Jiao,Yingyin Wu,Fangcheng Xu,Yan Li,Linchun Mao
Background Nuoshanyao (NSY) is a special species of Chinese yam ( Dioscorea opposita Thunb.) and shows rich functionality and superior nutritional value. The chemical components and structural properties of Nuoshanyao polysaccharide (NSYP) were investigated in his study. Results The molecular weight (Mw) of the major component in the polysaccharide was 23 kDa. NSYP was mainly composed of glucose, mannose, and galactose, and the molar ratio was 36.5:1.63:1. There might be an ordered helical conformation in NSYP, and the surface of NSYP was granular. NSYP exhibited strong scavenging capacity on the DPPH radical and chelating capacity on the ferrous ion in vitro with a rate of 94.94% and 82.49% at 4 mg/mL. Conclusion NSYP with the appropriate molecular weight range and helix structure could possess great antioxidant activity, while monosaccharide compositions may affect the activity. This work supplied important information for the potential application of NSYP in the nutritional and food industries.
研究领域:
食品科学
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生物化学
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营养学
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DOI
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 16
·9081
·
IF 6.208
Wei Liu,Xiaoge Wang,Danyang Zhou,Xiangze Fan,Jinhua Zhu,Xiuhua Liu
A novel hydrogel (DOP/PEI-PBA) based on the “three-component” reaction of 2-formylphenylboric acid (2-FPBA), the primary amine group of polyethyleneimine (PEI) and the cis-o-dihydroxy groups ofDioscorea oppositaThunb polysaccharide (DOP) was designed in this work. The hydrogel can be easily prepared by simply mixing the three reactants at room temperature. The hydrogel had dual responsiveness to glucose and pH, and can realize the controllable release of insulin. Moreover, the hydrogel combining insulin and DOP can inhibit the reactive oxygen species (ROS) level and malondialdehyde (MDA) content, and promote glucose consumption as well as the level of superoxide dismutase (SOD), in high-glucose-induced injury in HL-7702 cells, which reflects the synergistic effect of insulin and DOP to protect hepatocytes from oxidative stress at the same time. Further in vitro cytotoxicity studies showed that the hydrogel had good biocompatibility and no obvious toxicity to cells. These indicate that the prepared hydrogel (DOP/PEI-PBA) can be expected to be applied in the clinical treatment of insulin deficiency in diabetes.Keywords:Dioscorea oppositaThunb polysaccharide;hydrogel;insulin;drug delivery
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DOI
PubMed
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-11
·
IF 7.8
Zhang Yun,Sun Xinru,Lu Ang’ang,Lai Jianxia,Yang Yuran,Zhang Jie,Jaouhari Abdelhadi E. L.,Zhu Jinhua,Ma Fanyi
This study compared the effects of cooking processes (including steaming, boiling, hot-air roasting and microwaving) on the nutrients of Dioscorea opposita planting in sandy soil (SSCY) and loessial soil (LSCY). The contents of crude fiber and reducing sugar increased significantly during cooking for SSCY, whereas those of LSCY remained unchanged. A significant decline in allantoin content was observed in boiled yams (approximately 6.0% for SSCY and 14.1% for LSCY, respectively). Moreover, the differences in chemical compositions between SSCY and LSCY were compared using non-targeted metabolomics. Totally, 656 differential metabolites of LSCY were identified, including lipids, nucleotides, amino acids and derivatives, and organic acids, which might be the key reason why LSCY exhibits higher nutritional values than SSCY. This research contributed to understanding the systematic metabolic changes during planting and the molecular mechanisms influencing the quality formation, providing the theoretical references for the more scientific consumption of Chinese yam.
研究领域:
植物学
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食品科学
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土壤科学
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营养学
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DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 16
·2973
·
IF 3.06
Haiming Chen,Zhen Hu,Dongli Liu,Congfa Li,Sixin Liu
The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze–thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea oppositaThunb.) starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China. Our results show that all the CYYS gave a typical C-type X-ray diffraction pattern. The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84–4.55%. The freeze–thaw stability of each CYYS showed a contrary tendency with its amylose content. In addition, CYYS-3 showed the highestTo(81.1 °C),Tp(84.8 °C),Tc(91.2 °C), andΔH(14.1 J/g). The pasting temperature of CYYS-1 increased significantly with sucrose addition. NaCl could inhibit the swelling power of CYYS. There were significant decreases in pasting temperature and pasting time of CYYS when pH decreased.Keywords:Dioscorea oppositaplants;Chinese yam starch;pasting property;freeze–thaw stability
研究领域:
食品科学
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植物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Gang Wang,Dan Wang,Chuan Qing,Li Chen,Peng Gao,Min Huang
In this study, Chinese yam ( Dioscorea opposita Thunb. ) flour was irradiated at doses of 1.79, 6.06, 10.07 and 14.97 kGy of the electron beam, to analyze the effects of electron beam irradiated (EBI) on the solubility, transmittance, swelling, paste properties, and the microstructure of starch in yam flour. Results showed that EBI degraded the starch component of yam flour, and presented an increase in solubility from 15.59 to 30.05%, transmittance from 8.11 to 11.63%, and a decrease in swelling from 9.59 to 7.26 g/g. Also, we observed that there was a significant decrease in the paste properties of yam flour with the elevation of EBI. The scanning electron micrographs and Fourier transforms infrared spectra further illustrated the effect of EBI on the molecular structure, but not the surface structure of starch in yam flour. C -type crystalline structure in yam starch was also observed in both control and treatment, while irradiated yam starch had a lower crystallinity than that of unirradiated treatments. Yam starch exhibited a decrease trend in their thermal property values with irradiation treatment. This study concludes that the physical properties of yam flour instructed by EBI may be used in a wide application in the food and pharmaceutical industrie.
研究领域:
食品科学
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植物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2021
·
IF 7.514
Wei Tang,Dan Liu,Jun-Qiao Wang,Xiao-Jun Huang,Jun-Yi Yin,Fang Geng,Shao-Ping Nie
A low methyl-esterified pectin (33.2% methyl-esterification degree) was isolated from the tuber of Dioscorea opposita Thunb., which was an edible and medicinal material in China. This pectin ( M w of 1.3 × 10 4 g/mol) contained the ~59.1% homogalacturonan (HG) and ~38.1% highly branched rhamnogalacturonan I (RG-I) region with possible side chains embracing arabinogalactan II, arabinan or arabinogalactan I. The fragments including HG backbone consisting of → 4)-α-Gal p A-(1 → and → 4)-α-Gal p A-6- O -methyl-(1 → with molar ratio of ~2:1, and repeating unit of arabinogalactan II side chain composed of α-Ara f -(1 → and → 3,6)-β-Gal p -(1→, were speculated through methylation analysis and NMR spectra. However, the linkage pattern for RG-I backbone and side chains were indiscernible due to limited resolution of NMR spectra. Besides, the pectin adopted a flexible chain conformation in 0.1 M NaNO 3 solution. These results provided a structural basis for study on polysaccharide from D. opposite , which was benefit for development of functional food of yam.
研究领域:
食品科学
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植物化学
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多糖结构
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DOI
PubMed
高影响因子
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2018
·
IF 3.638
Songsong Zhang,Beibei Qiu,Jinhua Zhu,Weiping Hu,Fanyi Ma,M. Z. H. Khan,Xiuhua Liu
Present study aimed to immobilise the α-glucosidase on suitable supports to construct enzymatic microreactors and their subsequent applicability in efficient inhibitor screening from the Chinese Yam (Dioscorea opposita Thunb.) peel. A type of lamellar and porous composites (rGO@Fe3O4) were synthesised with a facile one-step solvothermal method and employed as carriers to construct enzymatic microreactors for screening α-glucosidase ligand from the Chinese Yam peel in league with the high performance liquid chromatography and mass spectrometry (HPLC-MS). The immobilisation amount of α-glucosidase on rGO@Fe3O4 under the optimised conditions was about 40 μg α-glucosidase/mg carriers. Furthermore, the binding capacities of screened inhibitors, 2,4-dimethoxy-6,7-dihydroxyphenanthrene and batatasin I, were 35.6 and 68.2%, respectively. Hence, considering their high screening efficiency and excellent magnetic separation ability, these as-prepared nanocomposite consisting of rGO and Fe3O4 may be potential supports for the enzyme (such as α-glucosidase) immobilisation for rapid α-glucosidase inhibitors screening from the diverse nature resources.
研究领域:
生物化学
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药物开发
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2025
·
IF 2.8
Zhen-Xing Wang,Li-Li Zhang,Xin-Huai Zhao
Yam (Dioscorea opposita Thunb.) is cultivated as one of the staple food crops, and its edible tubers are found to contain various bioactive substances, especially the so-called soluble and nondigestible yam polysaccharides (YPs). However, whether covalent Se-grafting of YP could lead to changed antioxidant and hypoglycemic activities remains unknown. YPs were thus separated from fresh Chinese yam tubers using water extraction and enzymatic treatment and then chemically selenized to yield two products, namely, YPSe-I and YPSe-II. These products acquired covalent Se-grafting in the form of selenite and had different Se contents (0.795 and 1.48 versus 0.035 g/kg) or selenization extents. The results obtained from the determination of antioxidant capacity indicated that the two Se-grafted products possessed a higher antioxidant activity compared to YP because they were more effective in scavenging both OH and ABTS radicals or reducing Fe3+ to Fe2+. Meanwhile, the two Se-grafted products, especially YPSe-II, had a higher capacity than YP to inhibit the activities of two digestive enzymes, namely, α-amylase and α-glucosidase. The results derived from cell experiments indicated that YP, YPSe-I, and YPSe-II were not cytotoxic to the normal BRL-3A liver cells and established insulin-resistant BRL-3A cells. However, YPSe-I and particularly YPSe-II exhibited a hypoglycemic effect in the model cells that was higher than that of YP, resulting in increased glucose consumption and enhanced oxidase activities in superoxide dismutase, catalase, and glutathione peroxidase. Data comparison results also manifested that YPSe-II consistently demonstrated higher antioxidant and hypoglycemic activities than YPSe-I. It was thus concluded that the covalent Se-grafting and high selenization extent endowed the Se-grafted YP products with enhanced beneficial functions, such as antioxidant and hypoglycemic effect. Meanwhile, this covalent Se-grafting could potentially serve as a suitable chemical approach to modify the two beneficial functions of natural polysaccharides.
研究领域:
食品科学
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营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
GLYCOCONJUGATE JOURNAL
·
2022
·Vol. 39
·期 1
·131-141
·
IF 3.009
Pan Hao-Yu,Zhang Chun-Qian,Zhang Xiu-Qi,Zeng Hui,Dong Chun-hong,Chen Xia,Ding Kan
Abstract Antibiotic-associated diarrhea (AAD) is a common side-effect of antibiotic treatment resulting from an imbalance in the colonic bacteria. The hypothesis of this study is to ask whether polysaccharide from the rhizome of Dioscorea opposita which is recorded as conventional herbs and food for diarrhea treatment in Southeast Asia, may be an active compound against diarrhea induced by antibiotics. To address, firstly, a homogenous polysaccharide, DOP0.2-S-3 was characterized as a homogalacturonan containing linear repeating units of → 4)-α- D -GalA p (1 → 4)-α- D -GalA p (1 → with the average molecular weight of 14 kDa. DOP0.2-S-3 significantly reduced the water content and defecation times caused by AAD in mice, while it also remarkably attenuated the cytokines of IL-1β and IL-6 expression in mice colon tissues. DOP0.2-S-3 decreased potential pathogen and increased Bacteroidetes in the mice gut. These results suggested DOP0.2-S-3 might be a new leading compound for the functional foods or drug candidate development against AAD partially through regulating gut flora. Graphical abstract
研究领域:
药理学
·
微生物学
·
免疫学
·
营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CARBOHYDRATE CHEMISTRY
·
2021
·
IF 1.667
Xuewei Jia,Xuanjing Wang,Yuanshang Liu,Yiyan Sun,Bingjie Ma,Zhenjie Li,Chunping Xu
An alkali-extracted polysaccharide (HY-B) was isolated and purified from Dioscorea opposita Thunb. (Huai Yam). Its chemical structure was analyzed with FT-IR, GC-MS and 1 D, 2 D-NMR spectrometry. It was proved that HY-B is mainly composed of α-1,4-linked glucose, which is similar to starch, but the starch-iodine color test proved that it was not starch. The chain conformation of HY-B was analyzed by SEC-MALLS-RI and TEM, indicated that HY-B exists in a random coil conformation. Its molecular weight is 18.7 kDa, and the molecular size is about 100 nm. MTT assays indicated that HY-B was nontoxic to RAW 264.7 cells in vitro. The results of anti-inflammatory studies showed that HY-B could inhibit the production of NO and TNF-α in RAW 264.7 macrophage stimulated by lipopolysaccharides (LPSs). This work provided the important information about the anti-inflammatory active component of Huai Yam and its potential applications in the food and health industries.
研究领域:
药理学
·
生物化学
·
分子生物学
·
食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）