Treg cells are crucial for maintaining immune homeostasis in CP/CPPS, but the molecular mechanisms underlying the modulation of the function of Treg in CP/CPPS remain unclear. The main purpose of this study is to investigate the relationship between immunosuppressive function of Treg and the methylation level of Foxp3 promoter in experimental autoimmune prostatitis (EAP) mouse model. EAP model was induced by subcutaneous injecting prostate-steroid-binding protein (PSBP) and complete Freund’s adjuvant with NOD mice. Histological analysis revealed that EAP model was successfully induced. The expression of IFN-γ was increased, and TGF-β was decreased in the serum of EAP, respectively. The percentage of Tregs in splenic lymphocyte was increased in EAP. The suppressive ability of Tregs on Teffs was impaired in EAP. The methylation level of Foxp3 promoter was increased, and the expression of Foxp3 was decreased in EAP. By injection AZA which was DNA-methylation inhibitor into EAP mice, prostate inflammation was alleviated, expressions of TGF-β and Foxp3 were increased, and the suppressive function of Tregs was improved in vitro and in vivo . Thus, we concluded that aberrant increased methylation of Foxp3 promoter in Treg cells leads to the impaired suppressive function of Treg cells, exacerbating autoimmune inflammatory injury in EAP.
Technological advances within the area of wireless sensor technology allow WSNs to be used in a increasing number of measurement scenarios. As new application areas are emerging, such as infrastructure monitoring and smart cities, the need for sensor mobility handling requires efficient and secure authentication protocols. This paper presents EAP-Swift, a novel EAP based authentication protocol with a focus on lightweight processing and faster response. It supports end-to-end session encryption key generation and mutual authentication. By utilizing lightweight hashing algorithms, the challenge-response authentication mechanism uses only two round trips to the AAA server for the complete authentication procedure leading to the reduction of latency by 33% compared to the baseline protocols. Further, using extensive experimentation, we validate that the authentication time can be kept below 250 ms and the power consumption can be kept below 15 mJ. Furthermore, we show that a battery lifetime of more than four years can be achieved when running the system on a regular button cell battery. Finally, the protocol was verified in terms of security using the AVISPA tool.
危险品化学品经营许可证（带存储）