SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Amin Zhang,Chongchong Miao,Hongyuan Shi,Hongkun Xiang,Chusen Huang,Nengqin Jia
Recent years have witnessed the great development of electrochemiluminescence (ECL) as a platform for the analytical processes in many fields, which includes pharmaceutical analysis, immunoassay, DNA analysis as well as clinical analysis. Herein, a novel electrochemiluminescence (ECL) sensor was fabricated for ultrasensitive detection of atropine based on Ru(bpy) 3 2+ /carbon nanospheres/Nafion composite film. The carbon nanospheres (CNPs) in composite film were obtained through a hydrothermal method. Then, electrochemical and ECL behaviors of the proposed sensor were investigated. Under the optimal conditions, the constructed atropine sensor showed a wide linear range from 0.5 to 250 μM with a detection limit of 0.17 μM (at signal-to-noise ratio of 3). Moreover, the results indicated that the CNPs-based ECL sensor exhibited excellent sensitivity and favorable stability, which might be ascribed to the high surface area and the good electrical conductivity of CNPs. Furthermore, the ECL sensor was applied for the determination of atropine in atropine sulfate injection samples and displayed a desirable result. The proposed ECL sensor could be easily obtained and be promising used for drug assay.
研究领域:
分析化学
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药学
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电化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
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2022
·Vol. 34
·期 10
·1555-1563
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IF 3.077
Ruirui Dong,Yanhui Zhang,Jiali Huang,Marhaba Habibul,Guixin Li
A novel electrochemiluminescence biosensor was presented for the assay of hepatitis B virus (HBV) DNA. Coralloid poly(aniline-luminol)-MWCNTs was prepared on an electrode surface, which was followed by the attachment of capture DNA via amide bond formation. The HBV-DNA and ferrocene labelled DNA hybridized with capture DNA to form a sandwich structure on electrode surface. Ferrocene can effectively increase the signal output of the ECL sensor due to the catalysis of ferrocene on electrochemiluminescence of luminol. The biosensor rendered a linear dependence over the range from 1.0×10 −16 to 1.0×10 −7 M with a low detection limit of 2.4×10 −17 M.
研究领域:
分析化学
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生物传感器
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病毒学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1395-1400
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IF 1.938
Li Wang,Di Luo,Dongdong Qin,Duoliang Shan,Xiaoquan Lu
This paper reports the electrochemiluminescence (ECL) behavior of CdSe/ZnS with K2S2O8 as the coreactant. The effects of pH, K2S2O8 concentration, the duration of the reaction of 4-ATP with GCE and the duration of the reaction of 4-ATP/GCE with QDs on ECL intensity were studied in detail. A possible ECL reaction mechanism was then proposed. In addition, it was observed that the ECL intensity was efficiently quenched by trace amounts of heavy metal ion. Based on the quenching effect of Pb2+ upon the ECL of CdSe/ZnS QDs, a QDs-ECL sensor was constructed to detect Pb2+ in preserved duck egg. The sensor showed good reproducibility and stability.
研究领域:
分析化学
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电化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-8
·
IF 5.3
Yang Shuning,Tian Li,Song Guanying,Li Huiling,Li Chao,Wu Qian,Shan Xiangyu,Zhao Lun
An electrochemiluminescence (ECL) sensor for determining bisphenol A (BPA) was prepared based on titanium dioxide (TiO 2 ) and Co-MOF. TiO 2 is a co-reaction promoter that amplifies the ECL signal in the Ru(bpy) 3 2+ -trinpropylamine (TPrA) system. When the electrode is modified with Co-MOF the ECL signal is significantly enhanced. This is because Co-MOF can not only be used as a co-reaction accelerator but also as a carrier to adsorb more luminescent substances. Possible mechanisms for amplifying the original signal through the synergistic action of the two substances are investigated. The ECL strength decreases with increasing concentrations of BPA, and the amount of BPA can be determined by the change in ECL signal strength (Δ I ). Under optimal experimental conditions, the linear range of BPA was 2.0 × 10 −10 to 2.0 × 10 −5 M, with a determination limit of 6.7 × 10 −11 M (3 σ / m ). The relative standard deviation (RSD) of the signal for ten consecutive measurements was 1.5%. The sensor can be used to detect BPA in bottled samples with recoveries of 96 to 105%. Graphical
研究领域:
分析化学
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环境科学
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材料科学
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电化学
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传感器技术
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Peijin Wang,Qian Wu,Caixia Wang,Xue Yang,Min Wei,Yuxin Pu,Mao Zhou,Mingxiao Zhang
In this work, Prussian blue nanocubes functionalized partial reduced graphene oxide (PBs-PrGO) was first employed as a new electrochemiluminescence (ECL) signal amplification strategy of perylenetetracarboxylic acid (PTCA), and the ECL signal of PBs-PrGO-PTCA composite presented 3-fold enhancement compared with PTCA. The Fe III and Fe II active site of Prussian blue nanocubes (PBs) could promote the reduction of K 2 S 2 O 8 to generate more sulfate radical anion (SO 4 − ), which enhanced the ECL signal of PTCA. The partial reduced graphene oxide (PrGO) not only used as the substrate for in-situ growing PBs, but also could assemble PTCA through π–π stacking. Besides, folic acid (FA) could effectively quench the ECL signal of PTCA. Based upon above, a rapid and effectual FA sensor was constructed. Under the optimum conditions, the ECL sensor exhibited an excellent linear relationship for FA determination with the limit of detection low as 0.719 nM. The constructed sensor applied in the determination of FA in tablets and human urine with satisfactory results, which could realize rapid and accurate FA analysis.
研究领域:
分析化学
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纳米材料
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 13
·1560-1564
·
IF 2.073
Yang Li,Liu Yang,Zhewei Peng,Chengzhi Huang,Yuanfang Li
In this contribution, an ultra-sensitive electrochemiluminescence (ECL) sensor was proposed for dopamine (DA) detection based on ruthenium(II) complex (tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate, Ru(bpy)32+) functionalized Zn(II) metal–organic frameworks (Ru-MOFs). The Ru-MOFs, with large internal surface areas and abundant Ru(bpy)32+ encapsulated effectively, could act as a suitable ECL emitter in a DA detection system. The leakage of Ru molecules in the encapsulation system could be effectively prevented, which resulted in stable ECL signals and significantly improved the ECL performance. On the basis of the novel Ru-MOFs as efficient ECL tags, the as-prepared simple and label-free ECL sensor was employed to quantify dopamine, which showed an excellent linear response from 0.1 pM to 10 nM and a detection limit of 0.024 pM. The simple and sensitive strategy might be expanded to other applications in analysis and detection fields based on functional metal–organic frameworks.
研究领域:
分析化学
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材料科学
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电化学
·
传感器
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Peipei Li,Luo Luo,Dan Cheng,Yan Sun,Youyu Zhang,Meiling Liu,Shouzhuo Yao
An electrochemiluminescence (ECL) sensor provides a sensitive and convenient method for early diagnosis of diseases; however, it is still a challenge to develop simple and sensitive sensing platforms based on efficient ECL signals and luminophore groups. Porphyrin-based metal–organic frameworks (MOFs) show great potential in ECL sensing; however, the mechanism and structure–activity relationship, as well as application, are rarely reported. Herein, hydrothermal reactions obtained porphyrin Zr-MOFs (PCN-222) with different specific surface areas, pore sizes, structures, and surface charge states by tuning the reaction time were developed, which served both as the ECL luminophore, coreaction promoter for S2O82–, and a connection in the ECL immunoassay. By progressively controlling the condition of the hydrothermal reaction, PCN-222 with large surface area-abundant micropores can be obtained, which has good conductivity and positively charged surfaces, obtaining excellent ECL performance. The ECL performance and the enhancement mechanism were investigated in detail. Using PCN-222-6h with the best ECL intensity as the immobilization matrix for the aptamer, a highly sensitive and selective assay for thrombin was developed. The decrease of the ECL signal was logarithmically linear with the concentration of thrombin in the range from 50 fg mL–1 to 100 pg mL–1 with a low detection limit of 2.48 fg/mL. This proposed strategy provides a brand new approach for tuning of the structures of MOFs as effective ECL signal probes, thus providing wider possibilities for effective ECL immunoassays in the detection of other biomarkers in diagnosis of diseases.
研究领域:
分析化学
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材料科学
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生物传感器
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生物医学工程
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yuanhong Xu,Tao Chen,Jing Sun,Jingquan Liu
Based on the flexible molecular engineering technique of reversible addition-fragmentation chain transfer (RAFT) polymerization, various polymers carrying positive or negative charges and different terminal groups such as pyrene or tertiary amine were synthesized for fabricating multifunctional solid-state electrochemiluminescent (ECL) sensors. Accordingly, the chemosensors immobilizing the ECL probe or co-immobilizing the ECL probe and the coreactant were realized for the quantification of small molecules (e.g., tripropylamine , tetracycline), and an aptasensor was developed for the specific and sensitive lysozyme assay (limit of detection: 0.1 ng/mL). All of the sensors were realized via a simple design exploiting the π−π stacking and electrostatic interactions . It was confirmed that the proposed strategy is simple but universal for the fabrication of versatile ECL sensors that showed simplicity, cost-effectiveness, high sensitivity, long-term stability, and excellent reproducibility.
研究领域:
材料科学
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分析化学
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生物传感器
·
聚合物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zirui Wang,Jilong Zheng,Min Li,Shujia Wang,Zhida Gao,Yan-Yan Song,Chenxi Zhao
Rapid and accurate detection of trace microbial pathogens is essential for human health, environmental monitoring, and food safety. Here, we report the development of a solid-state electrochemiluminescence (ECL) bacterial sensor based on a β-glycosidase (β-Gal)-triggered coreactant generation strategy. Leveraging a three-dimensional TiO2 nanotube array integrated with peroxidase-like MIL-53(Fe), glucose oxidase-like Au nanoparticles, and luminol, this sensor efficiently generates coreactants on-site, enabling sensitive glucose detection. As glucose is the primary product of β-Gal-catalyzed lactose hydrolysis, its concentration directly correlates with β-Gal enzymatic activity, thus allowing for the quantification of β-Gal via ECL signal intensity. Using enterohemorrhagic Escherichia coli (EHEC) as a model of food-borne pathogens, the sensor demonstrates rapid detection (30 min) with an ultralow LOD (0.8 CFU mL–1), representing the most sensitive label-free bacterial ECL sensor reported to date. Furthermore, the ECL electrode was applied for the detection of EHEC on raw salmon slices, underscoring its potential for food safety monitoring.
研究领域:
分析化学
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生物传感器
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食品安全
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纳米技术
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微生物学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）