ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2016
·Vol. 408
·期 25
·7095-7103
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IF 3.125
Yu Linpo,Liu Yang,Zhou Ming
Ruthenium(II) complexes with carboxylic acid as a bioconjugatable group, i.e., [Ru(bathophenanthroline disulfonate)(2,2′-bipyridine)(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)] 0 , (C 49 H 38 N 6 O 8 S 2 Ru), and [Ru(bathophenanthroline disulfonate) 2 (4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)] 2− · 2Na + , (C 63 H 44 N 6 O 14 S 4 RuNa 2 ) were characterized spectroscopically and electrochemically. As potential labels for electrochemiluminescence (ECL) immunoassays, the ECL intensities of the free labels in homogenous aqueous buffer solutions were compared under a condition that is similar to the one employed by a commercial clinical immunoassay system. The two labels were found to be more emissive and, thus, can be detected at 10 - 12 pM compared with 5× 10 −12 pM of the label currently used in the commercial ECL system. Furthermore, the improved ECL emission of the free labels in homogenous solutions was proven to be translated into more intense ECL signal in heterogeneous sandwich immunoassay and, thus, leading to a lower limit of detection in immunoassay. The data obtained from these ECL labels shed light on the further development of ECL-based clinical immunoassay technology.
Electrochemiluminescence immunoassays were carried out with three different ruthenium(II) complex labels. It was proved that the higher signal intensities found with the novel labels in homogeneous solutions were maintained in heterogeneous sandwich format.
研究领域:
分析化学
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生物技术
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临床诊断
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PubMed
高影响因子
Analytical Methods
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2014
·Vol. 7
·期 4
·1395-1400
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IF 1.938
Li Wang,Di Luo,Dongdong Qin,Duoliang Shan,Xiaoquan Lu
This paper reports the electrochemiluminescence (ECL) behavior of CdSe/ZnS with K2S2O8 as the coreactant. The effects of pH, K2S2O8 concentration, the duration of the reaction of 4-ATP with GCE and the duration of the reaction of 4-ATP/GCE with QDs on ECL intensity were studied in detail. A possible ECL reaction mechanism was then proposed. In addition, it was observed that the ECL intensity was efficiently quenched by trace amounts of heavy metal ion. Based on the quenching effect of Pb2+ upon the ECL of CdSe/ZnS QDs, a QDs-ECL sensor was constructed to detect Pb2+ in preserved duck egg. The sensor showed good reproducibility and stability.
研究领域:
分析化学
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电化学
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环境科学
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材料科学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Yingjie Li,Yuqin Li,Ning Xu,Jiahong Pan,Tufeng Chen,Yaowen Chen,Wenhua Gao
In the present study, the authors report a novel sandwich biosensor for detection of thrombin using electrochemiluminescence method based on dual-signal amplification strategy. Firstly, the gold nanoparticles graphene composite (GNPs-graphene) was obtained by simultaneously depositing the chloroauric (III) acid tetrahydrate [HAuCl 4 ·4H 2 O] and graphene solution with a cyclic voltammetry. Secondly, one thiol-modified thrombin aptamer (TBA1) was immobilized the glassy carbon electrode (GCE) via Au-S bonding. And then the other thiol-modified biotinylated thrombin aptamer (TBA2) that were modified with Ru(bpy) 3 2+ were anchored on the surface of the gold nanoparticles in solution. This proposed assay took dual signal amplification strategies. On the one hand, a single gold nanoparticle can connect many TBA2 to come into being a signal cluster (Ru-TBA2-AuNPs), thus when a little thrombin were present the system the ECL intensity will have a strong signal response; On the other hand, the GNPs-graphene can promote the electron transfer efficiency and increase the signal response. The ECL signal had a good linear relationship with thrombin concentration in range of 0.01–10 nM, and the detection limit for thrombin was determined to be as low as 6.3 pM. This biosensor also showed good selectivity for thrombin without being affected by some other proteins, such as BSA and lysozyme and so on. Moreover, this proposed method was successfully applied to thrombin analysis in diluted human serum samples.
研究领域:
生物化学
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分析化学
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纳米技术
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生物医学工程
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高影响因子
RSC Advances
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2016
·Vol. 6
·期 16
·13217-13223
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IF 3.289
Xiaomin Fu,Zhengcong Gu,Qiyi Lu,Jiayao Liao,Shihong Chen
C60/graphite-like carbon nitride nanosheet (C60/g-C3N4 NS) hybrids were prepared by a simple π–π interaction, and used to construct a solid state electrochemiluminescence (ECL) sensor. Based on the fact that melamine could efficiently quench the ECL intensity of g-C3N4 NS, such a sensor achieved detection of melamine. The possible quenching mechanisms were discussed. Due to the combination of the excellent ECL behavior of g-C3N4 NS with the outstanding electronic conductivity of C60, the prepared sensor exhibited a response for melamine with a wide linear range of 5.0 × 10−13 to 2.7 × 10−11 M and 2.7 × 10−11 to 1.9 × 10−8 M. The detection limit was 1.3 × 10−13 M. In this work, the linear range of melamine was improved by six orders of magnitude and the detection limit as low as sub-picomolar. Such a construction strategy provided a simple, sensitive, low-cost and green detection method for melamine.
研究领域:
分析化学
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材料科学
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食品安全检测
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高影响因子
ANALYTICAL SCIENCES
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2019
·Vol. 35
·期 8
·875-882
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IF 1.618
Wang Xia,Zhai Suyan,Liu Chao,Wang Xiaoshu,Yang Ya,Tu Yifeng
An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.
研究领域:
分析化学
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生物医学工程
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法医学
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药物成瘾研究
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高影响因子
LUMINESCENCE
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2021
·Vol. 37
·期 1
·58-71
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IF 2.464
Zheng Chang,Chao Zhang,Binghua Yao
A novel dual-sensitization electrochemiluminescence (ECL) immunosensor for the detection of tumour protein prostate specific antigen (PSA) at trace level using Ru(bpy) 3 2+ -doped chitosan/SiO 2 nanoparticles (Ru(bpy) 3 2+ /chitosan/SiO 2 NPs) as the first signal enhancers was fabricated. Due to chitosan with excellent pore forming capacity, these nanoparticles possess porous structures and better photopermeability, and therefore have higher luminescence efficiencies compared with Ru(bpy) 3 2+ /SiO 2 NPs reported in previous publications. Conversely, chitosan with good biocompatibility and high hydrophilicity was electrochemically decorated onto a Nafion/multiwall carbon nanotubes (Nafion/MWNTs) modified electrode surface and used as the second sensitizing matrix to seize large amounts of prostate specific capture antibody (Ab 1 ). The chitosan-decorated Nafion/MWNTs composites exhibited a 5.5-times higher ECL intensity than the unadorned Nafion/MWNTs films. Also, without additional reagents, such as (3-aminopropyl)triethoxysilane (APTS), the one-step functionalized Ru(bpy) 3 2+ /chitosan/SiO 2 NPs provided a large number of active arms to connect with PSA-detected antibodies (Ab 2 ) through the amino groups in chitosan. After a sandwich immunoreaction, the PSA antigen and Ru(bpy) 3 2+ /chitosan/SiO 2 NPs-labelled Ab 2 were sequentially captured onto the Ab 1 /chitosan/Nafion/MWNTs-modified electrode surface. The ECL signal increases were linearly related to the PSA antigen concentrations and ranged from 0.01 pg·mLl −1 to 10.0 pg·mLl −1 . Under the optimized experimental conditions, the immunosensor displayed excellent sensitivity and selectivity. The detection limit was as low as 3.4 fg·mLl −1 , equivalent to, or better than, those of the reported ECL immunosensors for PSA.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物医学工程
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PubMed
高影响因子
BIOELECTROCHEMISTRY
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2022
·
IF 5.76
Shuting Cheng,Rui Xu,Fengzhen Yang,Jingcheng Huang,Xia Sun,Xue Huang,He Li,Falan Li,Yemin Guo,Mohammad Hasanzadeh,Yelong Zhu
A novel sandwich electrochemiluminescence (ECL) aptasensor was developed for highly sensitive detection of kanamycin using luminol-functionalized aptamer as a signal probe. The aptasensor used polyethyleneimine (PAMAM), molybdenum disulfide, and multi-walled carbon nanotubes as the substrate, which provided enough binding sites for aptamer1 (the aptamer which modified NH 2 ) coupling. We found that kanamycin could be detected using the aptamer1 containing the same base sequence as aptamer2 (the aptamer which modified SH) on the electrode self-assembly. In addition, PAMAM nanocomposites can be used to effectively improve the ECL intensity by loading a high volume of luminol molecules and silver nanoparticles. In the presence of kanamycin, the sandwiched aptasensor was formed between aptamer1 and the probe of aptamer2 connecting silver nanoparticles, luminol, and PAMAM, resulting in a proportional increase of ECL intensity. Since the significantly enhanced loading of luminol by PAMAM accelerated the electron transfer, the sensitive aptasensor exhibited a wide linear range of detection from 1 × 10 -3 to 1 × 10 3 ng/mL and a low detection limit of 0.21 pg/mL (S/N) for kanamycin. The fabricated aptasensor was successfully applied in quantitative analysis of kanamycin in milk samples.
研究领域:
分析化学
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电化学
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生物传感器
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食品安全
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PubMed
高影响因子
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