BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yanxue Xu,Lei Zhang,Yuan Liu,Zhaoyu Jin,Qian Zhao,Feng Yang,Dan Xiao
Electrochemiluminescence (ECL) material Ru-dpq (Ru(bpy) 2 dpq 2+ , dpq=1,10-phenanthroline-5,6-dione; bpy=2,2′-bipyridine) is found to be produced strong and stable anodic ECL signal, which could be quenched by reduced glutathione (GSH) and exhibits high sensitivity and selectivity simultaneously. According to the mass spectra of Ru-SG (product of Ru-dpq reacted with GSH), and single crystal structure of the final oxidized product Ru-dcbpy ((Ru(bpy) 2 dcbpy 2+ , dcbpy=3,3-dicarboxy-2,2-bipyridine), we propose a new interacted mechanism between Ru-dpq and GSH. A good linear relation is estimated to be from 0.1 pM to 50 μM in the presence of calcium ion and the detection limit is as low as 0.087 pM (with the signal-to-noise ratio of 3). The relative standard deviation is 2.3% (for three repeated measurements). Furthermore, the ECL signal of Ru-dpq under a constant potential (1.2 V) is extremely stable and the intensity could be maintained over 600 s, which promotes us to determine the concentration of GSH via chronoamperometry .
研究领域:
分析化学
·
电化学
·
生物分析
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 15
·3702-3707
·
IF 3.864
Jun-Tao Cao,Fu-Rao Liu,Fang Hou,Juan Peng,Shu-Wei Ren,Yan-Ming Liu
The cathodic electrochemiluminescence (ECL) behaviour of nontoxic MoS2 quantum dots (QDs) was studied for the first time using potassium peroxydisulfate as the co-reactant. Ag-PAMAM NCs, serving as difunctional tags for quenching and enhancing ECL of MoS2-reduced graphene oxide composites, were introduced into the ECL detection system for signal amplification. By modulating the interparticle distance between MoS2 QDs and Ag-PAMAM NCs, the ECL quenching from resonance energy transfer and the ECL enhancement from surface plasma resonance were realized. Coupling the good ECL performance of MoS2 QDs with the excellent ECL quenching and enhancement effects of Ag-PAMAM NCs, a novel MoS2 QDs-based ECL biosensing platform for sensitive detection of microRNA-21 was achieved with a detection limit of 0.20 fmol L−1 (S/N = 3). This method was successfully applied to the determination of microRNA-21 in human serum samples with recoveries of 90.0–110.0%, suggesting great potential for its applications in biological and chemical analysis.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-10
·
IF 6.232
Huang Bo,Liu Xing-Pei,Chen Jing-Shuai,Mao Chang-jie,Niu He-Lin,Jin Bao-Kang
An electrochemiluminescence (ECL) biosensor was fabricated for the evaluation of prostate specific antigen (PSA). The sensor was developed by successively modifying glassy carbon electrode (GCE) electrodes with CdS/Chito/g-C 3 N 4 nanocomposites and DNA1 was labeled at the 5′ end with thiol. The aptamer DNA was labeled at the 3′ end with a quencher ferrocene (Fc) was ligated to DNA1 by the principle of complementary base pairing. In the absence of PSA, the ECL intensity signal is effectively quenches through the energy transfer and photoexcitation electron transfer between CdS/Chito/g-C 3 N 4 emitter and quencher Fc. After incubation with target PSA, the aptamer DNA interacts with PSA and then moved away from the electrode surface together, which will recover the ECL intensity. Under the optimal conditions, the ECL intensity increases linearly with the logarithm of PSA concentration in the range of 1 pg·mL −1 to 100 ng·mL −1 , and the detection limit is 0.14 pg·mL −1 (S/ N = 3). The biosensor has been successfully applied to the determination of PSA in serum sample.
Schematic representation of the electrochemiluminescence sensor based on a CdS/chitosan/g-C 3 N 4 nanocomposite, which can be applied to the determination of prostate specific antigen in serum.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaomin Fu,Yan Yang,Na Wang,Shihong Chen
Here, the cathodic electrochemiluminescence (ECL) behavior of 3,4,9,10-perylenetetracar-boxylic acid (PTCA) coreacted with hydrogen peroxide was firstly reported. The quenching effect of Fe-MIL-88 MOFs (iron based metal-organic framework) on the ECL of PTCA was found, and the ECL quenching mechanism was investigated, which was speculated to be via the resonance energy transfer from PTCA to Fe-MIL-88 MOFs and steric hindrance effect from Fe-MIL-88 MOFs. Combining with dual molecular recognition strategy and the quenching effect of Fe-MIL-88 MOFs on the ECL of PTCA-H 2 O 2 system, an “on-off” ECL sensor was designed to detect dopamine (DA) with a broad linear range (five orders of magnitude). The detection limit was low to 2.9 × 10 −13 M. The PTCA-H 2 O 2 system would provide a new ECL platform, especially for constructing enzyme based ECL sensor with H 2 O 2 as product in enzymatic reaction. Furthermore, the electrochemiluminescence resonance energy transfer between Fe-MIL-88 metal–organic framework and PTCA would expand a new application of Fe-MIL-88 MOFs in ECL field. The dual molecular recognition strategy ensured the selectivity of the sensor for DA detection.
研究领域:
分析化学
·
材料科学
·
电化学
·
传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）