TALANTA
·
2023
·
IF 6.1
Zixuan Ding,Peilin Wang,Zhenrun Li,Yupeng Guo,Qiang Ma
The combination of highly sensitive electrochemiluminescence (ECL) techniques with localized surface plasmon resonance (LSPR) effect can achieve the highly sensitive and specific detection in the analytical and biosensing applications. However, how to effectively improve the electromagnetic field intensity is an unresolved issue. Herein, we have developed an ECL biosensor based on sulfur dots and [email protected] nanorod array architecture. Firstly, the high luminescent sulfur dots with ionic liquid capping (S dots (IL) have been prepared as the new ECL emitter. The ionic liquid greatly improved the conductivity of sulfur dots in the sensing process. Furthermore, [email protected] nanorods array structure was constructed on the electrode surface by the evaporation induced self-assembly. On the one hand, the LSPR of [email protected] nanorods was more significant than that of good nanomaterial due to the plasma hybridization and the competition between free electrons and oscillating electrons. On the other hand, nanorods array structure had strong electromagnetic field intensity as hot spots due to the surface plasmon coupling ECL effect (SPC-ECL) effect. Therefore, the Au @Ag nanorods array architecture not only greatly enhanced the ECL intensity of sulfur dots, but also changed the ECL signals into polarized emission. Finally, the constructed polarized ECL sensing system was used to detect the mutated BRAF DNA in the eluent of thyroid tumor tissue. The biosensor showed the linear range from 100 fM to 10 nM with a detection limit of 20 fM. The satisfactory results demonstrated that the developed sensing strategy had great potential in the clinical diagnosis of BRAF DNA mutation in thyroid cancer.
研究领域:
分析化学
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生物传感
·
纳米技术
·
癌症诊断
·
生物医学工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Wenrong Cai,Yanjing Shi,Datong Wu,Junyao Li,Laidi Xu,Yong Kong
This work proposes a novel dual-signal electrochemiluminescence ( ECL) immunosensor based on energy resonance transfer (RET) for the detection of β2-Microglobulin (β2-MG). Introducing carbon vacancy into g-C 3 N 4 (V C -g-C 3 N 4 ) can lead to the narrowing of the band gap and the reduction of LUMO, thereby significantly enhancing the ECL efficiency of the material. Meanwhile, Zr-MOF can effectively load Ru(bpy) 3 2+ (Ru-Zr-MOF), enabling its stable existence on the electrode. The ECL emission spectrum of V C -g-C 3 N 4 overlaps well with the fluorescence excitation spectrum of Ru-Zr-MOF, indicating an effective ECL-RET effect between them. As the concentration of β2-MG increases, the intensity of anodic ECL signal gradually increases, while the intensity of cathodic ECL signal gradually decreases. The difference between the cathodic and anodic ECL signals has a good linear relationship within the concentration range from 0.2 pg/mL to 200 ng/mL, with a detection limit of 27.73 fg/mL. The constructed immunosensor can be used for practical analysis in human serum. The proposed sensor has great potential in clinical detection of β2-MG, providing new insights for biomolecular detection and disease diagnosis.
研究领域:
生物化学
·
分析化学
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生物医学工程
·
临床诊断
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DOI
高影响因子
ELECTROANALYSIS
·
2020
·Vol. 32
·期 6
·1155-1159
·
IF 2.544
Shuang Han,Yuan Gao,Lin Li,Beibei Lu,Yongxing Zou,Ling Zhang,Jiaheng Zhang
In this study, we found that glucose enhance electrochemiluminescence (ECL) intensity of both Au nanoclusters (Au NCs) and carbon quantum dots (CQDs) with K 2 S 2 O 8 as the co-reactants. The enhancing effects by Au NCs and CQDs were overlapped, enabling the detection of glucose. The increased ECL intensity of glucose was linear with the logarithm of concentrations of glucose in the range of 50 μM–3.0 mM, and the limit of detection is 20 μM. Anti-interruption ability was achieved, and ascorbic acid, urea, and uric acid had little influence to glucose detection. This method realized the direct detection of glucose by enhancing ECL of Au NCs and CQDs, which was fast and economic, possessing potential applications for glucose detection in human serum.
研究领域:
纳米技术
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分析化学
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生物医学工程
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yanxue Xu,Lei Zhang,Yuan Liu,Zhaoyu Jin,Qian Zhao,Feng Yang,Dan Xiao
Electrochemiluminescence (ECL) material Ru-dpq (Ru(bpy) 2 dpq 2+ , dpq=1,10-phenanthroline-5,6-dione; bpy=2,2′-bipyridine) is found to be produced strong and stable anodic ECL signal, which could be quenched by reduced glutathione (GSH) and exhibits high sensitivity and selectivity simultaneously. According to the mass spectra of Ru-SG (product of Ru-dpq reacted with GSH), and single crystal structure of the final oxidized product Ru-dcbpy ((Ru(bpy) 2 dcbpy 2+ , dcbpy=3,3-dicarboxy-2,2-bipyridine), we propose a new interacted mechanism between Ru-dpq and GSH. A good linear relation is estimated to be from 0.1 pM to 50 μM in the presence of calcium ion and the detection limit is as low as 0.087 pM (with the signal-to-noise ratio of 3). The relative standard deviation is 2.3% (for three repeated measurements). Furthermore, the ECL signal of Ru-dpq under a constant potential (1.2 V) is extremely stable and the intensity could be maintained over 600 s, which promotes us to determine the concentration of GSH via chronoamperometry .
研究领域:
分析化学
·
电化学
·
生物分析
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-10
·
IF 6.408
Wang Yuexin,Chen Rui,Shen Bo,Li Cai,Chen Junman,Wang Yanshuang,Tian Shen,Li Xuemiao,Luo Nini,Liu Rui,Ding Shijia,Zhu Chuanlong,Xia Qianfeng
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal–organic frameworks (Co-MOF), gold nanoparticles (AuNP), and N -(4-aminobutyl)- N -(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay of Burkholderia pseudomallei with the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination of B. pseudomallei in serum sample was as low as 9.0 CFU mL −1 . The relative standard deviations (RSD) of ECL intensity for the detection of five B. pseudomallei -spiked serum samples were 4.02%, 0.84%, 0.84%, 1.55%, and 0.21%, respectively. The recoveries of the ECL biosensor for the detection of B. pseudomallei DNA-spiked serum samples were 93.63 ~ 107.83%. Therefore, this work demonstrated that the developed multifunctional catalytic nanomaterial with synergistic and zero-distance catalytic properties can be used as excellent ECL signal reporter to improve the detection sensitivity of ECL biosensor. Graphical abstract
研究领域:
生物传感器
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分析化学
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纳米技术
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微生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Peilin Wang,Zizhun Wang,Zhenrun Li,Yuan Wang,Qiang Ma
In this work, a novel electrochemiluminescence (ECL) biosensor was fabricated to detect the mutant KRAS gene in the tumor tissues. Firstly, a new kind of chitosan-based dots was prepared and regulated by phenylboronic acid (PBA) as ECL nanoprobe. Furthermore, the electrochemically deposited Ag structures with controllable morphology have been developed on the electrode. The detailed electrochemical deposition condition of Ag structure has been investigated deeply. Due to the synergistic effect of the large active surface area and the high conductivity , the layered Ag flower-like structure with branches can significantly enhance the ECL intensity of CS-PBA dots by 12.1 times. Finally, the toehold mediated strand displacement strategy was employed in the ECL biosensor to quantitatively detect mutant KRAS gene with the range of 0.01 pM to 10 nM. The limit of detection was 3.3 fM. This biosensor has been used successfully to analyze the target DNA in the tumor tissues of actual cancer patients . The results showed the novel sensing platform possessed great potential for clinical analysis.
研究领域:
生物医学工程
·
分析化学
·
分子生物学
·
癌症研究
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yameng Li,Peilin Wang,Huan Wang,Zhenrun Li,Zihui Liang,Qiang Ma
MiRNA-214 can regulate the expression of their downstream target genes after post-transcriptional and are involved in the biological processes of triple negative breast cancer (TNBC). In this work, the small-sized luminescent Nb 2 C nanosheet-based whispering gallery mode-enhanced electrochemiluminescence (ECL) strategy was successfully constructed to detect miRNA-214 in TNBC. Firstly, we have synthesized small-sized luminescent Nb 2 C nanosheets from Nb 2 AlC MXene. The Nb 2 C nanosheets not only exhibited more stable chemical properties and reduced the defects of the large sheet structures, but also possessed the quantum confinement effect with the discrete energy level. As a result, the prepared small-sized Nb 2 C nanosheets had unique luminescent and electrochemical properties. Furthermore, in order to improve the ECL performance of Nb 2 C nanosheets, SiO 2 microspheres were self-assembled on the electrode surface by gas-liquid interface method to form whispering gallery mode structure. Because the light was continuously reflected at the interface of the microcavity in the whispering gallery mode, the ECL signal of Nb 2 C luminescent nanosheets was amplified largely. Finally, the whispering gallery mode-based ECL sensing platform was established. The results showed that the biosensor had a good linear correlation between the ECL intensity and the logarithm of concentration of miRNA-214 in the range of 10 fM to 100 nM with a limit of detection of 2.5 fM. The actual detection of miRNA-214 content in clinical TNBC tissue samples was realized successfully.
研究领域:
材料科学
·
分析化学
·
生物医学工程
·
癌症生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Binghui Li,Xin Wang,Xiaocui Yin,Huiting Hu,Guobing Wei,Jing Zhang,Hao Fan
A dual-mode electrochemiluminescence (ECL) and fluorescence (FL) probe was developed based on functionalized metal-organic framework nanosheets (RuMOFNSs) and (3-((2,4-dinitrophenyl)thio)phenyl)methanol (DNB) for the highly sensitive detection of hydrogen sulfide (H₂S) in homogeneous systems. Compared to conventional heterogeneous detection strategies, the homogeneous nature of this probe eliminates signal loss associated with solid-phase immobilization, thereby enhancing the interaction efficiency between the probe and target molecules. The developed sensing platform exhibited exceptional sensitivity, achieving detection limits of 0.18 pM in ECL and 0.4 nM in FL, with recovery rates ranging from 96.1 % to 101.7 %. Moreover, the probe demonstrated high selectivity against potential interfering metal ions and proved effective for monitoring food spoilage and dynamic H₂S fluctuations in rat brain tissue. Compared to existing detection approaches, this dual-mode probe offers superior sensitivity, selectivity, and broader applicability, underscoring its potential for environmental and biomedical analyse
研究领域:
分析化学
·
环境科学
·
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Xinli Wang,Hongyan Liu,Shaoqiang Zhang,Zhongcui Jing,Changkai Zhang,Songxia Yan,Hong Zhou,Haiyan Wang
Background Recently, various techniques have been developed to accurately and sensitively detect tumor biomarkers for the early diagnosis and effective therapy of cancer. The electrochemiluminescence (ECL) method holding outstanding features including high sensitivity, ease of operation, and spatiotemporal controllability exhibited great potential for DNA/RNA detection, immunoassay, cancer cell detection, and environmental analysis. However, a glaring problem of ECL approaches is that the layer-by-layer modification on the electrode leads to poor stability and sensitivity of the sensors. Therefore, new simple and efficient methods for electrode modification which can effectively improve the ECL signal have attracted more and more research interests. Results Based on the dual amplification strategy of target-induced CHA and nanocomposite probes leading to self-generated co-reactant (H 2 O 2 ), we proposed a highly sensitive miRNA-ECL detection system. The introduction of the target miRNA-21 triggers the CHA cycle amplification of DNA1 and biotin-modified DNA2, releasing the target miRNA-21 sequence for the target cycle reaction. After the reaction, the newly introduced DNA2 was combined with Au NPs modified with SA and Glucose oxidase (GOD). In the presence of oxygen, glucose was decomposed by GOD to produce H 2 O 2 , and then H 2 O 2 was immediately catalyzed by the Hemin/G-quadruplex at the double-stranded end of the CHA product to produce a large amount of O 2 −• . As a co-reactant of luminol, the ECL signal was significantly enhanced, thereby achieving highly sensitive detection of miRNA-21 content and obtaining a low detection limit of 0.65 fM. The high specificity of the ECL biosensor was also proved by base mismatch. Significance Compared with other current detection methods, this sensor can achieve quantitative analysis of other target analytes by flexibly changing the probe DNA sequence, and provide a new feasible solution for the detection of tumor-associated markers. Benefiting from the improved sensitivity and selectivity, the proposed biosensing platform is expected to provide a new strategy for biomarkers analysis and outstanding prospect for further clinical application.
研究领域:
生物医学工程
·
分析化学
·
分子生物学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 7
·2359-2366
·
IF 4.019
Xia Xu,Xiangxiang Qin,Ling Wang,Xiaofang Wang,Jing Lu,Xia Qiu,Yinggui Zhu
In this study, a new lanthanide terbium complex, Tb(pzda)3(NO3)3·nH2O, was synthesized by a hydrothermal method and characterized by Fourier transform infrared spectroscopy (FT-IR) and energy-dispersive X-ray spectroscopy (EDS). It was found that the as-synthesized Tb-complex exhibited good electrochemiluminescence (ECL) behavior in the presence of triethanolamine (TEOA) in a HAc-NaAc buffer solution on a glassy carbon electrode. The possible reaction mechanism has been discussed based on the fluorescence spectra and ECL spectra. For sensing applications, it was found that protocatechuic acid (PCA) had an obvious quenching effect on the ECL signal of the Tb-complex, and this resulted in a decreased ECL signal associated with the concentration of PCA. Therefore, a highly sensitive method for the detection of PCA was established with a linear range of 1.283 × 10−10 M to 3.845 × 10−4 M and a detection limit of 0.085 nM at an S/N ratio of 3. This novel ECL assay strategy with an outstanding ECL efficiency offers great potential for pharmaceutical analyses.
研究领域:
分析化学
·
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Yiting Shao,Xuemei Wang,Hanxi Liu,Jianfei Xia,Feifei Zhang,Zonghua Wang
The electrochemiluminescence (ECL) system of Luminol is frequently constrained by its sluggish reaction kinetics and strong dependence on dissolved oxygen in practical applications. Herein, we report an innovative strategy for enhanced oxygen evolution reaction (OER) catalysis mediated by defect engineering, which provides a new insight into boosting the kinetic process of ECL performance in the Luminol/H 2 O system. In this study, Ferrocene monocarboxylic acid (FcCA) was coordinatively linked to the topological framework of nickel-based metal-organic frameworks (NiBDC MOFs) to construct Fc-NiBDC MOFs, achieving a significant improvement in the OER performance, leading to a 4-fold increase in the ECL intensity of Luminol. This is mainly because FcCA induces coordination defects at adjacent Ni sites, creating a high density of oxygen vacancies to facilitate the conversion of reactive oxygen species (ROS). At the same time, by utilizing its strong adsorption ability for hydroxyl groups, it can significantly increase the local concentration of ROS at the active sites. Furthermore, the introduced Fc/Fc + redox cycle continuously accelerates the generation and transformation of ROS, thereby constructing an efficient ROS cyclic regeneration mechanism. This triple synergistic enhancement effect can effectively promote the sustained generation and efficient enrichment of ROS, thereby significantly improving the ECL efficiency of Luminol immobilized on NiBDC MOFs through amide bonds. The biosensor constructed on this basis was applied for the highly sensitive detection of E.coli , with a limit of detection (LOD) as low as 0.33 CFU mL −1 . This strategy pioneers a new pathway for the defect engineering in MOF-based ECL systems, offering a versatile platform for pathogen diagnostics.
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1395-1400
·
IF 1.938
Li Wang,Di Luo,Dongdong Qin,Duoliang Shan,Xiaoquan Lu
This paper reports the electrochemiluminescence (ECL) behavior of CdSe/ZnS with K2S2O8 as the coreactant. The effects of pH, K2S2O8 concentration, the duration of the reaction of 4-ATP with GCE and the duration of the reaction of 4-ATP/GCE with QDs on ECL intensity were studied in detail. A possible ECL reaction mechanism was then proposed. In addition, it was observed that the ECL intensity was efficiently quenched by trace amounts of heavy metal ion. Based on the quenching effect of Pb2+ upon the ECL of CdSe/ZnS QDs, a QDs-ECL sensor was constructed to detect Pb2+ in preserved duck egg. The sensor showed good reproducibility and stability.
研究领域:
分析化学
·
电化学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
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