ACS Omega
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2025
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IF 4.3
Mengfan Duan,Zhuo Wang,Weiping Zhu,Yufang Xu,Xuhong Qian
The recent discovery of heavy metal pollution in Chinese herbs has raised serious concerns for both human health and the environment. Researchers’ attention has been drawn to the toxicity and bioaccumulation of heavy metal ions. In this paper, a new technical method for preparing ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid (EGTA)-functionalized cotton fiber was innovatively proposed, and on this basis, heavy metal ions were successfully removed. First, the surface of cotton fiber (FC) was modified with amino group by reactive dye dyeing method to obtain FN (amino-modified cotton fiber). Subsequently, EGTA anhydride was grafted onto FN to obtain FNE (EGTA-modified cotton fiber). The interaction between materials and metal ions was systematically studied by isothermal titration calorimetry and other methods. The results showed that the adsorption capacity of FNE for Cd2+, Pb2+, and Hg2+ could reach 18.87, 25.84, and 6.69 mg/g, respectively. More importantly, FNE can be reused at least five times in an acidic environment. Furthermore, it is possible to efficiently eliminate heavy metal ions from Chinese herb extracts without compromising the active ingredients. This research provides a brand-new idea and method for solving the problem of removing heavy metal ions from Chinese herb extracts.
研究领域:
环境科学
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材料科学
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生物学
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DOI
PubMed
高影响因子
Environmental Technology & Innovation
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2020
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IF 3.356
Jiahong Wang,Saleem Atif,Dan Zhang
EGTA functionalized magnetic microsphere (Fe 3 O 4 @SiO 2 @EGTA) was prepared for the removal of Cr(III) from highly saline organic wastewater environment. The synthesized adsorbent was characterized with Fourier transform infrared (FTIR), powder x-ray diffraction (XRD), thermogravimetric analysis (TGA) and x-ray photoelectron spectrometry (XPS). The characterization techniques depicted the successful surface modification of magnetic particles (Fe 3 O 4 @SiO 2 ) with the functional groups of EGTA without changing its crystal structure. The maximum adsorption amount of Cr(III) was 25.25 mg ⋅ g −1 at pH 4.0. The Langmuir isotherm was well fitted on the adsorption data, while adsorption equilibrium was achieved in first 15 min, and the equilibrium data well explained by pseudo second order model. The smaller concentrations of inorganic cations (Na + , Ca 2＋ , Mg 2 + ), organic acids (EDTA, Citric acid , Formic acid) and complexing agent with high salt system reduce the adsorption capacity to some extent, but after that the higher concentrations of these competing species have no apparent effect on the adsorption performance. According to the XPS analysis, the carboxyl and amino groups were responsible for the adsorption of Cr(III), while the main mechanism of adsorption were coordination complex and electrostatic interaction. The prepared adsorbent can be readily isolated by external magnetic field and regenerated for several times and still possess higher adsorption performance.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2014
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IF 3.552
Zhichao Sun,Yunguo Liu,Yuanqing Huang,Xiaofei Tan,Guangming Zeng,Xinjiang Hu,Zhongzhu Yang
In this study, the removal of Cd 2+ and Pb 2+ from aqueous solutions was investigated using a novel chelating material. The first part described the synthesis of ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid dianhydride (EGTAD), mercerization treatment of ramie fiber (MRF), and the MRF was then reacted with EGTAD to prepare the new material (ERF). The obtained material was characterized by weight gain, SEM, FTIR, and elemental analysis. The results of FTIR and elemental analysis confirmed that ester bond, carboxyl and amine groups were introduced onto ERF. The adsorption capacity of metals on ERF was evaluated at different contact times, pH values, initial metal concentrations, and temperatures in the second part. The adsorption equilibrium was reached within 5 min for Cd 2+ and Pb 2+ . Adsorption isotherm could be well fitted by the Langmuir model, and the maximum adsorption capacities were 159.11 and 273.78 mg g −1 for Cd 2+ and Pb 2+ at 298 K, respectively. Thermodynamic analysis showed that the adsorption process was spontaneous and endothermic. The molar ratio of adsorbed cation to grafted EGTA is close to 1.8:1, which confirmed that the adsorption was chemical process involving both surface chelation reaction and ion exchange. In addition, the absorbent was successfully regenerated using HCl and ultrasonic treatment.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2016
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IF 3.15
Pengfei Xu,Zhiwei Shen,Baolin Zhang,Jun Wang,Renhua Wu
Superparamagnetic iron oxide nanoparticles (SPIONs) as T2 contrast agents have great potential to sense calcium ion (Ca 2+ ) using magnetic resonance imaging (MRI). Here we prepared calcium-responsive SPIONs for MRI, formed by combining poly(ethylene glycol) (PEG) and polyethylenimine (PEI) coated iron oxide nanoparticle (PEI/PEG-SPIONs) contrast agents with the straightforward calcium-sensing compound EGTA (ethylene glycol tetraacetic acid). EGTA was conjugated onto PEI/PEG-SPIONs using EDC/sulfo-NHS method. EGTA-SPIONs were characterized using TEM, XPS, DSL, TGA and SQUIID. DSL results show that the SPIONs aggregate in the presence of Ca 2+ . MRI analyses indicate that the water proton T2 relaxation rates in HEPES suspensions of the EGTA-SPIONs significantly increase with the calcium concentration because the SPIONs aggregate in the presence of Ca 2+ . The T2 values decreased 25% when Ca 2+ concentration decreased from 1.2 to 0.8 mM. The aggregation of EGTA-SPIONs could be reversed by EDTA. EGTA-SPIONs have potential as smart contrast agents for Ca 2+ -sensitive MRI.
研究领域:
医学影像学
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纳米医学
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生物材料学
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DOI
高影响因子
Materials Today Bio
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2025
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IF 10.2
Jianxin Li,Yingchen Ni,Yanjie Tao,Hao Cai,Anqi Wu,Fengyuan Zhang,Shijie Meng,Yuyang Luo,Weidong Zhang,Youhua Wang
Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by progressive joint degradation, with mitochondrial dysfunction significantly contributing to its pathogenesis. Despite extensive research into therapeutic strategies, successfully addressing mitochondrial dysfunction in RA poses a significant challenge. This paper presents an innovative functionalization method, deploying hydrocaffeic acid-modified chitosan, in conjunction with the selective calcium chelator ethylene glycol bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA), and incorporating stromal cell-derived factor 1 alpha (SDF-1α). In vitro , this functionalized hydrogel exhibited a significant decrease in intracellular reactive oxygen species (ROS) levels, stabilization of mitochondrial membrane potential, mitigation of calcium overload, inhibition of mitochondrial dysfunction-induced cellular senescence, and a reduction in the release of senescence-associated secretory phenotype components. In vivo , this hydrogel effectively modulated immune responses and aided cartilage repair in a collagen-induced arthritis rat model. From a mechanistic perspective, high-throughput sequencing suggests that the therapeutic efficacy of this hydrogel may be associated with its ability to modulate mitochondrial function and inflammatory pathways. In summary, the hydrocaffeic acid- and EGTA-based functionalization strategy provides an innovative and straightforward process for integrating multiple functionalities into a single delivery platform, demonstrating the potential for tissue regeneration applications extending beyond RA.
研究领域:
生物医学工程
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风湿病学
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免疫学
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组织工程
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药学
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线粒体生物学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2023
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IF 8
Xiao Lei,Guodong Tan,Yiming Wang,Li Chen,Yuan Cao,Bingxin Si,Zhen Zhen,Bei Li,Yan Jin,Wei Wang,Fang Jin
Introduction Osteoarthritis (OA) is a chronic degenerative joint disease accompanied by an elevated macrophage proinflammatory phenotype, which is triggered by persistent pathologically elevated calcium ion levels in mitochondria. However, existing pharmacological compounds targeting the inhibition of mitochondrial calcium ion (m[Ca2+]) influx are currently limited in terms of plasma membrane permeability and low specificity for ion channels and transporters. In the present study, we synthesized mesoporous silica nanoparticle-amidated (MSN)-ethylenebis (oxyethylenenitrilo)tetraacetic acid (EGTA)/triphenylphosphine (TPP)-polyethylene glycol (PEG) [METP] nanoparticles (NPs), which specifically target mitochondria and block excess calcium ion influx.Methods m[Ca2+] overload in OA mouse bone marrow-derived macrophages (BMDMs) was detected by a fluorescence probe. A tissue in situ fluorescence colocalization assay was used to evaluate METP NP uptake by macrophages. BMDMs from healthy mice were pretreated with a concentration gradient of METP NPs followed by lipopolysaccharide (LPS) stimulation and detection of m[Ca2+] levels in vitro. The optimal METP NP concentration was further applied, and the endoplasmic reticulum (ER) and cytoplasm calcium levels were detected. The inflammatory phenotype was measured by surface markers, cytokine secretion and intracellular inflammatory gene/protein expression. A Seahorse cell energy metabolism assay was performed to elucidate the mechanism by which METP NPs reverse the BMDM proinflammatory phenotype.Results The present study identified calcium overload in BMDM mitochondria of OA mice. We demonstrated that METP NPs reversed the increased m[Ca2+] levels in mitochondria and the proinflammatory phenotype of BMDMs, with both in vivo and in vitro experiments, via the inhibition of the mitochondrial aspartate-arginosuccinate shunt and ROS production.Conclusion We demonstrated that METP NPs are effective and highly specific regulators of m[Ca2+] overload. In addition, we demonstrated that these METP NPs reverse the macrophage proinflammatory phenotype by restoring m[Ca2+] homeostasis, thereby inhibiting the tissue inflammatory response and achieving a therapeutic effect for OA.
研究领域:
药理学
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纳米技术
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免疫学
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骨科
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细胞生物学
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DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
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2018
·Vol. 24
·期 24
·6458-6463
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IF 5.16
Xinyue Song,Zihong Yue,Jiayu Zhang,Yanxialei Jiang,Zonghua Wang,Shusheng Zhang
Graphical By taking advantage of a lack of spectral overlapping and single NIR excitation wavelengths, sandwich-structured multicolor upconversion nanoparticles (SWUCNPs) were used to establish a dual luminescence resonance energy transfer (LRET) nanoprobe with special recognition ligands. The quenched fluorescence would be recovered after the detachment and/or absorbance change of the attached ligands (see scheme) to investigate the interplaying roles of [Ca 2+ ] i and pH i . Intracellular [Ca 2+ ] i and pH i have a close relationship, and their abnormal levels can result in cell dysfunction and accompanying diseases. Thus, simultaneous determination of [Ca 2+ ] i and pH i can more accurately investigate complex biological processes in an integrated platform. Herein, multicolor upconversion nanoparticles (UCNPs) were prepared with the advantages of no spectral overlapping, single NIR excitation wavelengths, and greater tissue penetration depth. The upconversion nanoprobes were easily prepared by the attachment of two fluorescent dyes, Fluo-4 and SNARF-4F. Based on the dual luminescence resonance energy transfer (LRET) process, the blue and green fluorescence of the UCNPs were specially quenched and selectively recovered after the detachment and/or absorbance change of the attached fluorescent dyes, enabling dual detection. Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca 2+ ] i and pH i change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells.
研究领域:
生物化学
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分子生物学
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纳米技术
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细胞生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）